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The map label for this gene is alkJ [H]

Identifier: 146342597

GI number: 146342597

Start: 5963455

End: 5965089

Strand: Reverse

Name: alkJ [H]

Synonym: BRADO5760

Alternate gene names: 146342597

Gene position: 5965089-5963455 (Counterclockwise)

Preceding gene: 146342598

Following gene: 146342595

Centisome position: 80.0

GC content: 67.52

Gene sequence:

>1635_bases
ATGGATACGTTCGATTATGTGATCGTGGGCGCCGGCTCCGCCGGCTGCGTGATCGCCAGCCGCCTCAGCGAGGACCCGTC
GGTGACGGTGTGCGTGCTGGAAGCGGGTCCGCGCGACTGGCATCCCTACATCCATCTGCCGGCCGGCTTCATCAAGACAT
TCTACATGAAGAGCATCAATTGGGGCTACCAGCAGGAGCCGGGGCCCTACACAGCCGGCCGCAGCATCTACGCGCCGCGC
GGCAAGACGCTCGGCGGCTCGTCCTCGATCAACGGCCACGTCTACAACCGCGGCCAGAGCCAGGATTTCGACACCTGGGC
ACAGCTGGGCAATCGCGGCTGGAGCTATTCCGACGTGCTGCCGCACTTCCGGCGCATGGAGAAGCGCATCGGCGCCGGCG
ACGAGCAGTATCGCGGCCGCGACGGCAGTCTTCATGTCACCACCATGGAGTGGAAGGACACGCTGTGCGAAGCCTTCATG
GACGGCGCCGTGTCGCTCGGCATTCCGCGCAATCCCGACTACAACGGCGCCATCCAGGAAGGCGTCTCCTACGTCCAGCG
CACCATCCAGAACGGCCGCCGCGTGTCCTCGGCGACGGCGTTCCTGAAGCCCGCCAGCAAGCGTCCGAACGTCGAGATCC
GAACCCACGCGCATGCCACGAGCATCATGCTCGAAGGCAAGCGCGCTGTCGGCGTGCGCTATCACCGCGGCGGACGCGGC
GGCGTGCCCGTCGAGGTGCGCGCGCGGAAAGAGGTCATCCTGTCCGGCGGCACCTACAACTCGCCGCAGCTGCTGCAGCT
CTCCGGCATCGGCGCGCCCGAGCTGCTGCAGGAGCACGGCATCGAAGTGCGGCACGCGTTGAAGAGCGTCGGCGAGGGCC
TGCAGGATCACTACGCGCCGCGCACGGTGGCGCGCGTCAAGAACATCAAGACCATCAACGAGCTCGCGCGCGGCCTCAAT
CTGTGGGGCGAGGCGCTGAAATGGGCGGTGACGCGCAAGGGCATCCTGTCGCTGTCGCCGACCATGGTCTACTGCTTCTG
GCATTCCGGCGAGACCGCCGAGAGCTCCGATCTGCAGCTCACCTTCACGCCGGCGAGCTACAAGGAGGGCGTGCAGGGCC
AACTCGAGGACGAGCCGGGCATGACGGTGGCGTCCTGGCAGCAGCGCCCGGAGAGCCGCGGCTACGTGCGCATCCGCTCC
GCCGATCCGTTCGCGCCGCCGCTGATTCAGACCAACTACCTTACCGCCGAGCTCGACCGCCGCGTCATCGTCGCCGGTAT
GAAGCTGGCGCGCCGGCTGCTCGCCTCGGCCCCGCTCGCGCCATACTACGCGTATGAGGACTTCCCAGGTCCGAAGGTGA
ACAGCGACGACGAATTCCTCGCCGCCGCCACCCAACGCGCCACCACCACTTTCCATCCCGGCTGCACCTGCCGCATGGGA
CCGGCCGAGTCCACCTGGGCCACCGTCGACGACCAGCTCCGCGTCCACGGCCTGCAGGGCCTCCGCATCGCCGACGCCTC
GATCATGCCGCGCATGATCTCGGCGAACCTCAACGCCGCCACGCTGATGATCGGCGACAGGGCGGCGGATCTGATTCTGG
GCAAGGTGCAGGCGGGGGAGAGGTTGTCGGCCTAA

Upstream 100 bases:

>100_bases
GCATCTCGATTATCAACTGCATGACAAGCCGCGCTGATCGCGCTATGGCCGCCGTGCCGCGCCACGCGGCCAGCATGACG
AGACCGGGACGACAGACGAG

Downstream 100 bases:

>100_bases
CCCCGGCCGTTGCCGCTTATCGTTCGAGCCCGCGTCATGCCGCACGACCAGTGCGCCCCCCTCCCCCTTGCGGGGAGGGG
TCGGGGGTGGGGGTCCCCGA

Product: putative choline (or alcohol) dehydrogenase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MDTFDYVIVGAGSAGCVIASRLSEDPSVTVCVLEAGPRDWHPYIHLPAGFIKTFYMKSINWGYQQEPGPYTAGRSIYAPR
GKTLGGSSSINGHVYNRGQSQDFDTWAQLGNRGWSYSDVLPHFRRMEKRIGAGDEQYRGRDGSLHVTTMEWKDTLCEAFM
DGAVSLGIPRNPDYNGAIQEGVSYVQRTIQNGRRVSSATAFLKPASKRPNVEIRTHAHATSIMLEGKRAVGVRYHRGGRG
GVPVEVRARKEVILSGGTYNSPQLLQLSGIGAPELLQEHGIEVRHALKSVGEGLQDHYAPRTVARVKNIKTINELARGLN
LWGEALKWAVTRKGILSLSPTMVYCFWHSGETAESSDLQLTFTPASYKEGVQGQLEDEPGMTVASWQQRPESRGYVRIRS
ADPFAPPLIQTNYLTAELDRRVIVAGMKLARRLLASAPLAPYYAYEDFPGPKVNSDDEFLAAATQRATTTFHPGCTCRMG
PAESTWATVDDQLRVHGLQGLRIADASIMPRMISANLNAATLMIGDRAADLILGKVQAGERLSA

Sequences:

>Translated_544_residues
MDTFDYVIVGAGSAGCVIASRLSEDPSVTVCVLEAGPRDWHPYIHLPAGFIKTFYMKSINWGYQQEPGPYTAGRSIYAPR
GKTLGGSSSINGHVYNRGQSQDFDTWAQLGNRGWSYSDVLPHFRRMEKRIGAGDEQYRGRDGSLHVTTMEWKDTLCEAFM
DGAVSLGIPRNPDYNGAIQEGVSYVQRTIQNGRRVSSATAFLKPASKRPNVEIRTHAHATSIMLEGKRAVGVRYHRGGRG
GVPVEVRARKEVILSGGTYNSPQLLQLSGIGAPELLQEHGIEVRHALKSVGEGLQDHYAPRTVARVKNIKTINELARGLN
LWGEALKWAVTRKGILSLSPTMVYCFWHSGETAESSDLQLTFTPASYKEGVQGQLEDEPGMTVASWQQRPESRGYVRIRS
ADPFAPPLIQTNYLTAELDRRVIVAGMKLARRLLASAPLAPYYAYEDFPGPKVNSDDEFLAAATQRATTTFHPGCTCRMG
PAESTWATVDDQLRVHGLQGLRIADASIMPRMISANLNAATLMIGDRAADLILGKVQAGERLSA
>Mature_544_residues
MDTFDYVIVGAGSAGCVIASRLSEDPSVTVCVLEAGPRDWHPYIHLPAGFIKTFYMKSINWGYQQEPGPYTAGRSIYAPR
GKTLGGSSSINGHVYNRGQSQDFDTWAQLGNRGWSYSDVLPHFRRMEKRIGAGDEQYRGRDGSLHVTTMEWKDTLCEAFM
DGAVSLGIPRNPDYNGAIQEGVSYVQRTIQNGRRVSSATAFLKPASKRPNVEIRTHAHATSIMLEGKRAVGVRYHRGGRG
GVPVEVRARKEVILSGGTYNSPQLLQLSGIGAPELLQEHGIEVRHALKSVGEGLQDHYAPRTVARVKNIKTINELARGLN
LWGEALKWAVTRKGILSLSPTMVYCFWHSGETAESSDLQLTFTPASYKEGVQGQLEDEPGMTVASWQQRPESRGYVRIRS
ADPFAPPLIQTNYLTAELDRRVIVAGMKLARRLLASAPLAPYYAYEDFPGPKVNSDDEFLAAATQRATTTFHPGCTCRMG
PAESTWATVDDQLRVHGLQGLRIADASIMPRMISANLNAATLMIGDRAADLILGKVQAGERLSA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=546, Percent_Identity=39.3772893772894, Blast_Score=356, Evalue=3e-98,
Organism=Escherichia coli, GI1786503, Length=537, Percent_Identity=39.4785847299814, Blast_Score=348, Evalue=7e-97,
Organism=Caenorhabditis elegans, GI17532301, Length=550, Percent_Identity=33.0909090909091, Blast_Score=317, Evalue=9e-87,
Organism=Drosophila melanogaster, GI24642048, Length=571, Percent_Identity=34.5008756567426, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI24642059, Length=577, Percent_Identity=33.9688041594454, Blast_Score=291, Evalue=9e-79,
Organism=Drosophila melanogaster, GI17137792, Length=551, Percent_Identity=34.8457350272232, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24642042, Length=566, Percent_Identity=34.2756183745583, Blast_Score=289, Evalue=4e-78,
Organism=Drosophila melanogaster, GI45551458, Length=577, Percent_Identity=34.4887348353553, Blast_Score=289, Evalue=4e-78,
Organism=Drosophila melanogaster, GI45549471, Length=577, Percent_Identity=34.4887348353553, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI24650267, Length=567, Percent_Identity=34.2151675485009, Blast_Score=288, Evalue=7e-78,
Organism=Drosophila melanogaster, GI24642035, Length=576, Percent_Identity=35.0694444444444, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24642055, Length=572, Percent_Identity=33.9160839160839, Blast_Score=281, Evalue=8e-76,
Organism=Drosophila melanogaster, GI24642039, Length=563, Percent_Identity=34.2806394316163, Blast_Score=281, Evalue=1e-75,
Organism=Drosophila melanogaster, GI18859995, Length=568, Percent_Identity=34.1549295774648, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24642037, Length=579, Percent_Identity=33.8514680483592, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI24642051, Length=582, Percent_Identity=32.8178694158076, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI18859993, Length=572, Percent_Identity=30.7692307692308, Blast_Score=223, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24642057, Length=603, Percent_Identity=27.1973466003317, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24645930, Length=585, Percent_Identity=28.3760683760684, Blast_Score=142, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 59692; Mature: 59692

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTFDYVIVGAGSAGCVIASRLSEDPSVTVCVLEAGPRDWHPYIHLPAGFIKTFYMKSIN
CCCCEEEEEECCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCHHHHHHHHHHHCC
WGYQQEPGPYTAGRSIYAPRGKTLGGSSSINGHVYNRGQSQDFDTWAQLGNRGWSYSDVL
CCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCCCCHHHHH
PHFRRMEKRIGAGDEQYRGRDGSLHVTTMEWKDTLCEAFMDGAVSLGIPRNPDYNGAIQE
HHHHHHHHHHCCCCHHHCCCCCCEEEEEEHHHHHHHHHHHCCCEEECCCCCCCCCCHHHH
GVSYVQRTIQNGRRVSSATAFLKPASKRPNVEIRTHAHATSIMLEGKRAVGVRYHRGGRG
HHHHHHHHHHCCCEEHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCCEEEEEEECCCCC
GVPVEVRARKEVILSGGTYNSPQLLQLSGIGAPELLQEHGIEVRHALKSVGEGLQDHYAP
CCCEEEEECEEEEEECCCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHCCHHHHCCC
RTVARVKNIKTINELARGLNLWGEALKWAVTRKGILSLSPTMVYCFWHSGETAESSDLQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEEECCCCCCCCCEEE
TFTPASYKEGVQGQLEDEPGMTVASWQQRPESRGYVRIRSADPFAPPLIQTNYLTAELDR
EECCHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCCEECCEEEHHHCC
RVIVAGMKLARRLLASAPLAPYYAYEDFPGPKVNSDDEFLAAATQRATTTFHPGCTCRMG
CHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCEEECCCCEEECC
PAESTWATVDDQLRVHGLQGLRIADASIMPRMISANLNAATLMIGDRAADLILGKVQAGE
CCCCCCCCCCCHHEEECCCCEEEECHHHHHHHHHCCCCEEEEEECCCHHHHEEECCCCCC
RLSA
CCCC
>Mature Secondary Structure
MDTFDYVIVGAGSAGCVIASRLSEDPSVTVCVLEAGPRDWHPYIHLPAGFIKTFYMKSIN
CCCCEEEEEECCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCHHHHHHHHHHHCC
WGYQQEPGPYTAGRSIYAPRGKTLGGSSSINGHVYNRGQSQDFDTWAQLGNRGWSYSDVL
CCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCCCCHHHHH
PHFRRMEKRIGAGDEQYRGRDGSLHVTTMEWKDTLCEAFMDGAVSLGIPRNPDYNGAIQE
HHHHHHHHHHCCCCHHHCCCCCCEEEEEEHHHHHHHHHHHCCCEEECCCCCCCCCCHHHH
GVSYVQRTIQNGRRVSSATAFLKPASKRPNVEIRTHAHATSIMLEGKRAVGVRYHRGGRG
HHHHHHHHHHCCCEEHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCCEEEEEEECCCCC
GVPVEVRARKEVILSGGTYNSPQLLQLSGIGAPELLQEHGIEVRHALKSVGEGLQDHYAP
CCCEEEEECEEEEEECCCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHCCHHHHCCC
RTVARVKNIKTINELARGLNLWGEALKWAVTRKGILSLSPTMVYCFWHSGETAESSDLQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEEECCCCCCCCCEEE
TFTPASYKEGVQGQLEDEPGMTVASWQQRPESRGYVRIRSADPFAPPLIQTNYLTAELDR
EECCHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCCEECCEEEHHHCC
RVIVAGMKLARRLLASAPLAPYYAYEDFPGPKVNSDDEFLAAATQRATTTFHPGCTCRMG
CHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCEEECCCCEEECC
PAESTWATVDDQLRVHGLQGLRIADASIMPRMISANLNAATLMIGDRAADLILGKVQAGE
CCCCCCCCCCCHHEEECCCCEEEECHHHHHHHHHCCCCEEEEEECCCHHHHEEECCCCCC
RLSA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]