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The map label for this gene is sseA [H]

Identifier: 146342581

GI number: 146342581

Start: 5940792

End: 5941649

Strand: Reverse

Name: sseA [H]

Synonym: BRADO5744

Alternate gene names: 146342581

Gene position: 5941649-5940792 (Counterclockwise)

Preceding gene: 146342582

Following gene: 146342579

Centisome position: 79.68

GC content: 68.3

Gene sequence:

>858_bases
ATGACTACCCCCAACGACCCGCTCGTCTCCACGGACTGGCTCGCAGCGCATCTCGACGATCCCAAGATCAAGGTGCTCGA
CGCCACCTTCAAGATGCCCGGCGTGCTGCCGCTGCCGAAGGACGACTATCTGAAGGCGCACATTCCCGGCGCGGTGTTCT
TCGACGTCGACGAGGTCTCCGATCATTCCAATCCGCTGCCGCACATGTATCCGAGCGCGGATCAGTTCGGCCGTGATGCG
GCACGGCTCGGCGTCAGCAACGATGACACCGTGGTGGTCTACGATGCCGGCGGCTGGGTCGCCGCGCCGCGCGCCTGGTG
GATGTTCCTGTCGTTCGGCCACAAGGACGTTCGCGTGCTCGACGGCGGCCTGAAGAAGTGGATCGCCGAGGGCAGGCCGG
TCGACAGCGGGCAGGTGACCCCTGCGGCCGGCACCTACATCGCAAGCTTAGACGCCGCGCGTGTGCGCAAGGTCGAGCAG
ATGGTCGCGAACCTCACCGGCGGCGCCGAGCAGGTGATCGACGCCCGGCAGGCGCCGCGCTTTGCCGGCGAGGTGGCCGA
GCCCCGGCCCGGCCTGCGCTCCGGCCACATTCCGGGCAGCCGCAATCTGCCTTATGCGGAACTGTTCGATGCCGCCACCG
GCGTCATGAAGCCGCTCGATGAGCTTCGCAAGGCATTCGGCGCAGCCGGCGTCGATCTCGGCAAGCCGATCGTGACCTCG
TGCGGGTCGGGTGTCTCGGCAGCGGTGCTGACGCTGGCGCTCTACCGGCTCGGTCTGCGCGACACCGCGCTCTACGACGG
CTCCTGGTCGGAATGGGGCGTCGAGAACGGGCCGCCGGTCGCAACCGGGCCAGCCTGA

Upstream 100 bases:

>100_bases
GTCACACGCGGGCTTGACCCGCGTGTCTATCGCTTCGACAAGATGGATCGCCGGATCAAGTCCGGCGATGACGAATGTGA
AGTGATGCTGGAGCCAGGCC

Downstream 100 bases:

>100_bases
TCATCGCCTCTATCGCGTGCGCGGTCCGGCGGCCTGCTGGCCGATCGCTGCCGGCTGAGCGAACTGCTGGCTGAACGGAT
CGGGGAAGGCTTGCTGTTGC

Product: 3-mercaptopyruvate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTTPNDPLVSTDWLAAHLDDPKIKVLDATFKMPGVLPLPKDDYLKAHIPGAVFFDVDEVSDHSNPLPHMYPSADQFGRDA
ARLGVSNDDTVVVYDAGGWVAAPRAWWMFLSFGHKDVRVLDGGLKKWIAEGRPVDSGQVTPAAGTYIASLDAARVRKVEQ
MVANLTGGAEQVIDARQAPRFAGEVAEPRPGLRSGHIPGSRNLPYAELFDAATGVMKPLDELRKAFGAAGVDLGKPIVTS
CGSGVSAAVLTLALYRLGLRDTALYDGSWSEWGVENGPPVATGPA

Sequences:

>Translated_285_residues
MTTPNDPLVSTDWLAAHLDDPKIKVLDATFKMPGVLPLPKDDYLKAHIPGAVFFDVDEVSDHSNPLPHMYPSADQFGRDA
ARLGVSNDDTVVVYDAGGWVAAPRAWWMFLSFGHKDVRVLDGGLKKWIAEGRPVDSGQVTPAAGTYIASLDAARVRKVEQ
MVANLTGGAEQVIDARQAPRFAGEVAEPRPGLRSGHIPGSRNLPYAELFDAATGVMKPLDELRKAFGAAGVDLGKPIVTS
CGSGVSAAVLTLALYRLGLRDTALYDGSWSEWGVENGPPVATGPA
>Mature_284_residues
TTPNDPLVSTDWLAAHLDDPKIKVLDATFKMPGVLPLPKDDYLKAHIPGAVFFDVDEVSDHSNPLPHMYPSADQFGRDAA
RLGVSNDDTVVVYDAGGWVAAPRAWWMFLSFGHKDVRVLDGGLKKWIAEGRPVDSGQVTPAAGTYIASLDAARVRKVEQM
VANLTGGAEQVIDARQAPRFAGEVAEPRPGLRSGHIPGSRNLPYAELFDAATGVMKPLDELRKAFGAAGVDLGKPIVTSC
GSGVSAAVLTLALYRLGLRDTALYDGSWSEWGVENGPPVATGPA

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=277, Percent_Identity=41.8772563176895, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI61835204, Length=277, Percent_Identity=41.8772563176895, Blast_Score=225, Evalue=3e-59,
Organism=Homo sapiens, GI194473681, Length=277, Percent_Identity=41.8772563176895, Blast_Score=225, Evalue=3e-59,
Organism=Homo sapiens, GI17402865, Length=281, Percent_Identity=42.7046263345196, Blast_Score=213, Evalue=2e-55,
Organism=Escherichia coli, GI87082121, Length=274, Percent_Identity=45.985401459854, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI87081967, Length=271, Percent_Identity=29.1512915129151, Blast_Score=94, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17561888, Length=295, Percent_Identity=30.5084745762712, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71997283, Length=294, Percent_Identity=28.9115646258503, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI115534702, Length=240, Percent_Identity=30.8333333333333, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17543836, Length=291, Percent_Identity=30.2405498281787, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17543838, Length=291, Percent_Identity=30.2405498281787, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17559150, Length=273, Percent_Identity=28.2051282051282, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324825, Length=276, Percent_Identity=34.4202898550725, Blast_Score=156, Evalue=4e-39,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 30355; Mature: 30223

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPNDPLVSTDWLAAHLDDPKIKVLDATFKMPGVLPLPKDDYLKAHIPGAVFFDVDEVS
CCCCCCCCCCCCHHEEECCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCEEEEEHHHHC
DHSNPLPHMYPSADQFGRDAARLGVSNDDTVVVYDAGGWVAAPRAWWMFLSFGHKDVRVL
CCCCCCCCCCCCHHHHCCHHHHCCCCCCCEEEEEECCCCEECCHHHHHHHHCCCCCHHHH
DGGLKKWIAEGRPVDSGQVTPAAGTYIASLDAARVRKVEQMVANLTGGAEQVIDARQAPR
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCH
FAGEVAEPRPGLRSGHIPGSRNLPYAELFDAATGVMKPLDELRKAFGAAGVDLGKPIVTS
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
CGSGVSAAVLTLALYRLGLRDTALYDGSWSEWGVENGPPVATGPA
CCCCHHHHHHHHHHHHHCCCHHEEECCCCHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTPNDPLVSTDWLAAHLDDPKIKVLDATFKMPGVLPLPKDDYLKAHIPGAVFFDVDEVS
CCCCCCCCCCCHHEEECCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCEEEEEHHHHC
DHSNPLPHMYPSADQFGRDAARLGVSNDDTVVVYDAGGWVAAPRAWWMFLSFGHKDVRVL
CCCCCCCCCCCCHHHHCCHHHHCCCCCCCEEEEEECCCCEECCHHHHHHHHCCCCCHHHH
DGGLKKWIAEGRPVDSGQVTPAAGTYIASLDAARVRKVEQMVANLTGGAEQVIDARQAPR
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCH
FAGEVAEPRPGLRSGHIPGSRNLPYAELFDAATGVMKPLDELRKAFGAAGVDLGKPIVTS
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
CGSGVSAAVLTLALYRLGLRDTALYDGSWSEWGVENGPPVATGPA
CCCCHHHHHHHHHHHHHCCCHHEEECCCCHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]