Click here to switch to the map view.

The map label for this gene is cysU [H]

Identifier: 146342209

GI number: 146342209

Start: 5567873

End: 5568688

Strand: Reverse

Name: cysU [H]

Synonym: BRADO5361

Alternate gene names: 146342209

Gene position: 5568688-5567873 (Counterclockwise)

Preceding gene: 146342210

Following gene: 146342208

Centisome position: 74.68

GC content: 67.89

Gene sequence:

>816_bases
ATGTCGCACAAATCCTTCGTCTGGGCGTGTCTGTTGCCATTGGCGGCCGTGACGGCCGCCTTCTTCGTTCTGCCGGTGGT
GCATCTGCTGGTCACCGGCGCGGAGGGGCTGCGCGGACCGGCGGAGTATCTCGCCATCATCGCCGAGCCGCGCTATCGCG
CCACGCTCCTGAGCACGGTGCTGCTGGCGGCCGCGACCACGGTCGCGACGCTGATCGTCGCGACGATCGCCGGCCTGTTC
CTGCAGCGTCATCGCTTTCCGGGCCGTGCCGTTCTGGTGGCGATCCTGACCTTTCCGCTCGCGTTCCCCGGCGTCGTGAT
CGGCTTCATGATCATCCTGCTCGCCGGCCGCCAGGGCCTGATCGGCGACGTCTCCTCGCGTCTGCTCGGCGACAAGATCG
TATTCGCCTATTCGATCTACGGCCTGTTCCTGGGCTATCTCTACTTCTCGATTCCCAGGGTCATTCTCACCATCATGGCG
GCGGCGCAAAAGCTTGATCCGAGCCTGGAGGAGGCGGCCCGCTCGCTCGGCGCGAGCCCGTGGGCCGTGCTGCGCGACGT
CGTCCTGCCGGCGCTGGCGCCTGCGCTGGTCGCCTCGGGCGCCATCGCCTTTGCAACCGCAATGGGCGCCTTCGGCACGG
CGTTCACGCTCGCGACCAACATCGACGTGCTGCCGATGCTGATCTACACGGAGTTCACGCTGGCCGGAAACCTGGCGACC
TCGGCGGCGCTGTCGGTCGGCCTGGGCGCGATGACGTGGCTGATCCTGGCGCTGGCGCGCAGCTTCACCGGCAACTCCGT
GGCAGCGGCGGGCTAG

Upstream 100 bases:

>100_bases
AGGCCGCGCAGAAGGGCTTCGTCGACCGCTATCTGTCCGAAGTCCGCTAAGGGAGTCGCGTGCCCCGCTGGCCAACAGCC
GACGGGGCACGCTCGAGATC

Downstream 100 bases:

>100_bases
CACGTGCGCAACCGGATTCTCTTCACGCTTCAATTTCTGTTCACGCTGCTCGTCGCTGCGTTTCTGGCGGTGCCTGCCGG
ACTCTCGATTGCCGCGGGCG

Product: putative permease membrane protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSHKSFVWACLLPLAAVTAAFFVLPVVHLLVTGAEGLRGPAEYLAIIAEPRYRATLLSTVLLAAATTVATLIVATIAGLF
LQRHRFPGRAVLVAILTFPLAFPGVVIGFMIILLAGRQGLIGDVSSRLLGDKIVFAYSIYGLFLGYLYFSIPRVILTIMA
AAQKLDPSLEEAARSLGASPWAVLRDVVLPALAPALVASGAIAFATAMGAFGTAFTLATNIDVLPMLIYTEFTLAGNLAT
SAALSVGLGAMTWLILALARSFTGNSVAAAG

Sequences:

>Translated_271_residues
MSHKSFVWACLLPLAAVTAAFFVLPVVHLLVTGAEGLRGPAEYLAIIAEPRYRATLLSTVLLAAATTVATLIVATIAGLF
LQRHRFPGRAVLVAILTFPLAFPGVVIGFMIILLAGRQGLIGDVSSRLLGDKIVFAYSIYGLFLGYLYFSIPRVILTIMA
AAQKLDPSLEEAARSLGASPWAVLRDVVLPALAPALVASGAIAFATAMGAFGTAFTLATNIDVLPMLIYTEFTLAGNLAT
SAALSVGLGAMTWLILALARSFTGNSVAAAG
>Mature_270_residues
SHKSFVWACLLPLAAVTAAFFVLPVVHLLVTGAEGLRGPAEYLAIIAEPRYRATLLSTVLLAAATTVATLIVATIAGLFL
QRHRFPGRAVLVAILTFPLAFPGVVIGFMIILLAGRQGLIGDVSSRLLGDKIVFAYSIYGLFLGYLYFSIPRVILTIMAA
AQKLDPSLEEAARSLGASPWAVLRDVVLPALAPALVASGAIAFATAMGAFGTAFTLATNIDVLPMLIYTEFTLAGNLATS
AALSVGLGAMTWLILALARSFTGNSVAAAG

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=214, Percent_Identity=34.1121495327103, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786980, Length=205, Percent_Identity=32.1951219512195, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082099, Length=237, Percent_Identity=25.3164556962025, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787368, Length=167, Percent_Identity=26.9461077844311, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28235; Mature: 28104

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKSFVWACLLPLAAVTAAFFVLPVVHLLVTGAEGLRGPAEYLAIIAEPRYRATLLSTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHH
LLAAATTVATLIVATIAGLFLQRHRFPGRAVLVAILTFPLAFPGVVIGFMIILLAGRQGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IGDVSSRLLGDKIVFAYSIYGLFLGYLYFSIPRVILTIMAAAQKLDPSLEEAARSLGASP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCH
WAVLRDVVLPALAPALVASGAIAFATAMGAFGTAFTLATNIDVLPMLIYTEFTLAGNLAT
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
SAALSVGLGAMTWLILALARSFTGNSVAAAG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SHKSFVWACLLPLAAVTAAFFVLPVVHLLVTGAEGLRGPAEYLAIIAEPRYRATLLSTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHH
LLAAATTVATLIVATIAGLFLQRHRFPGRAVLVAILTFPLAFPGVVIGFMIILLAGRQGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IGDVSSRLLGDKIVFAYSIYGLFLGYLYFSIPRVILTIMAAAQKLDPSLEEAARSLGASP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCH
WAVLRDVVLPALAPALVASGAIAFATAMGAFGTAFTLATNIDVLPMLIYTEFTLAGNLAT
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
SAALSVGLGAMTWLILALARSFTGNSVAAAG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]