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The map label for this gene is phbC [H]

Identifier: 146342160

GI number: 146342160

Start: 5524672

End: 5526432

Strand: Reverse

Name: phbC [H]

Synonym: BRADO5310

Alternate gene names: 146342160

Gene position: 5526432-5524672 (Counterclockwise)

Preceding gene: 146342161

Following gene: 146342157

Centisome position: 74.11

GC content: 60.48

Gene sequence:

>1761_bases
GTGACTGATGTCAGGCTGCTCGCTCAGGACGATGTCGGCGGAGAGTTTGGGCGCGAGGATCACGAACTCGACAAGGCCTT
CAGCGCGGTTCTTGCCCGCGTGACCGGCGGAATCTCCCCGGCAGCGTTGTCGATGGCTTACCTGGACTGGGCGTGCCATC
TGGCGGCCGCCCCCCAGCGGCAGCTCGACAATGTGCGTCGCGCGTGGGATGGCGTAACGCAACTCGCCGAACGGTCGCTC
CATTTCGCCGATCCCAACCGTGGTCCCTGGGACCTGATCAAGCCGCAGGCCAATGATCACCGATTTTCCAAGCCGCAATG
GGGTATGCAGCCGTTCAACCTGTTTGCCCAGGCCTTCCTGCTCGGCGAAGATTGGTGGCACAAGGCCACGACGAACATTC
GCGGTGTCAATCCGGCCAACGAAGCCGTGGTCGACTTCTCGGTCCGTCAGCTGCTCGACATGTTCGCCCCGTCCAACTTT
GCAGTGACGAACCCCGAGGTCGTGGAAAAAACTTTCCAGACCGGCGGCGAAAACCTCGTCTTCGGCTGGCAGAATTGGCT
CGCCGATCTCATGCAGGTCTTGCAGCCGGGAAGAACGTCGGAAAAAGCCGAGTTCTTGCCCGGTCAGACGGTTGCGACGG
CGCCCGGCAAGATCGTGCTCCGCAATCGGTTGATCGAGCTGATACAATACGCGCCGACGACCCGGCAGGTGCGTCCCGAG
CCGATCCTGATCGTGCCGGCCTGGATCATGAAATACTACATTCTCGACCTGTCGCACTACAACTCGTTGGTCAGGTATCT
GACGGACCAGGGCTTCACGGTGTTCATGATCTCCTGGCGCAATCCGGGCGCCAGAGATCGCGATATAGCCTTCGACGACT
ATCGCTCGACCGGTGTCATGGCGGCCTTGGACGAAATCGGGAAAATCGTTCCGAATGCGCCGGTTCATGCGGCCGGCTAC
TGCCTCGGAGGCACGCTGCTCTCGATCACTGCGGCTGCGATGGCGCGCGAGGGTGATACCCGTTTGAAGACGATCACGTT
GTTTGCCGCACAGACCGATTTCACCGAAGCGGGAGAGCTCACTCTCTTCATCAACGAAAGTCAGGTCGCCTTCCTTGAAG
ACATGATGTGGGAGCGCGGCTATCTCGATACGACGCAGATGGCTGGAGCGTTCCAGTTGCTGCGATCCAACGATCTGATC
TGGTCACGCGTTTCGCGCGACTATCTGCTCGGCGAGCGGGCTCATCCGAGCGATCTGATGGCATGGAATGCCGACGCGAC
ACGGCTTCCGTATCGGATGCACTCTGAATACCTGCGCAAGCTGTTTCTGAACAACGATCTGGCCGAGGGACGGTATCGCG
TTCAGGGGAAGCCGGTGTCTCTCTCCGACATCCACACGCCGATGTTCGTCGTCGGAACGCTGCGCGACCACGTCGCGCCG
TGGAAATCGACGTACAAGATCCATTACGAGGTGGATGCCAACGTCACGTTCGTCCTGGCAAGTGGCGGTCACAATGCAGG
CATCGTCGCGCCGCCCGATGAGCAGGGACATTCGCATCAGGTACGCACCAAGGCGGCCGATGAGCCCTATCTCGGGCCCG
ACGAATGGCAGAGCACATCCCCGCACATAGAGGGATCCTGGTGGCCGACATGGATCAGCTGGCTTGCTCAACATTCGGAA
GCGCTGACTATTCCGCCGCGACTTGGAACGGATGACGGACATGAGCTTGGGGACGCGCCAGGCGAGTATGTGCACACGTG
A

Upstream 100 bases:

>100_bases
GAAGAGTACGAGTGTATCTCGTTCGGATCGCGAAGAACAGAATCAGCGCGGGCAGACGAGCATCGTCGAAATATCTCCGG
CAGCCGGCGGTCACCTCGGC

Downstream 100 bases:

>100_bases
GGTCGTCCTTCGGCACAGTTCGCCACGGGTTTGGCCATGAGTCCAGAAGCCTCAGTCTGACCTGCCGATCCGCAACACTG
ACAGGCTTTTGTCCAGCGTG

Product: putative poly-beta-hydroxybutyrate polymerase

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MTDVRLLAQDDVGGEFGREDHELDKAFSAVLARVTGGISPAALSMAYLDWACHLAAAPQRQLDNVRRAWDGVTQLAERSL
HFADPNRGPWDLIKPQANDHRFSKPQWGMQPFNLFAQAFLLGEDWWHKATTNIRGVNPANEAVVDFSVRQLLDMFAPSNF
AVTNPEVVEKTFQTGGENLVFGWQNWLADLMQVLQPGRTSEKAEFLPGQTVATAPGKIVLRNRLIELIQYAPTTRQVRPE
PILIVPAWIMKYYILDLSHYNSLVRYLTDQGFTVFMISWRNPGARDRDIAFDDYRSTGVMAALDEIGKIVPNAPVHAAGY
CLGGTLLSITAAAMAREGDTRLKTITLFAAQTDFTEAGELTLFINESQVAFLEDMMWERGYLDTTQMAGAFQLLRSNDLI
WSRVSRDYLLGERAHPSDLMAWNADATRLPYRMHSEYLRKLFLNNDLAEGRYRVQGKPVSLSDIHTPMFVVGTLRDHVAP
WKSTYKIHYEVDANVTFVLASGGHNAGIVAPPDEQGHSHQVRTKAADEPYLGPDEWQSTSPHIEGSWWPTWISWLAQHSE
ALTIPPRLGTDDGHELGDAPGEYVHT

Sequences:

>Translated_586_residues
MTDVRLLAQDDVGGEFGREDHELDKAFSAVLARVTGGISPAALSMAYLDWACHLAAAPQRQLDNVRRAWDGVTQLAERSL
HFADPNRGPWDLIKPQANDHRFSKPQWGMQPFNLFAQAFLLGEDWWHKATTNIRGVNPANEAVVDFSVRQLLDMFAPSNF
AVTNPEVVEKTFQTGGENLVFGWQNWLADLMQVLQPGRTSEKAEFLPGQTVATAPGKIVLRNRLIELIQYAPTTRQVRPE
PILIVPAWIMKYYILDLSHYNSLVRYLTDQGFTVFMISWRNPGARDRDIAFDDYRSTGVMAALDEIGKIVPNAPVHAAGY
CLGGTLLSITAAAMAREGDTRLKTITLFAAQTDFTEAGELTLFINESQVAFLEDMMWERGYLDTTQMAGAFQLLRSNDLI
WSRVSRDYLLGERAHPSDLMAWNADATRLPYRMHSEYLRKLFLNNDLAEGRYRVQGKPVSLSDIHTPMFVVGTLRDHVAP
WKSTYKIHYEVDANVTFVLASGGHNAGIVAPPDEQGHSHQVRTKAADEPYLGPDEWQSTSPHIEGSWWPTWISWLAQHSE
ALTIPPRLGTDDGHELGDAPGEYVHT
>Mature_585_residues
TDVRLLAQDDVGGEFGREDHELDKAFSAVLARVTGGISPAALSMAYLDWACHLAAAPQRQLDNVRRAWDGVTQLAERSLH
FADPNRGPWDLIKPQANDHRFSKPQWGMQPFNLFAQAFLLGEDWWHKATTNIRGVNPANEAVVDFSVRQLLDMFAPSNFA
VTNPEVVEKTFQTGGENLVFGWQNWLADLMQVLQPGRTSEKAEFLPGQTVATAPGKIVLRNRLIELIQYAPTTRQVRPEP
ILIVPAWIMKYYILDLSHYNSLVRYLTDQGFTVFMISWRNPGARDRDIAFDDYRSTGVMAALDEIGKIVPNAPVHAAGYC
LGGTLLSITAAAMAREGDTRLKTITLFAAQTDFTEAGELTLFINESQVAFLEDMMWERGYLDTTQMAGAFQLLRSNDLIW
SRVSRDYLLGERAHPSDLMAWNADATRLPYRMHSEYLRKLFLNNDLAEGRYRVQGKPVSLSDIHTPMFVVGTLRDHVAPW
KSTYKIHYEVDANVTFVLASGGHNAGIVAPPDEQGHSHQVRTKAADEPYLGPDEWQSTSPHIEGSWWPTWISWLAQHSEA
LTIPPRLGTDDGHELGDAPGEYVHT

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 65755; Mature: 65624

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVRLLAQDDVGGEFGREDHELDKAFSAVLARVTGGISPAALSMAYLDWACHLAAAPQR
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHH
QLDNVRRAWDGVTQLAERSLHFADPNRGPWDLIKPQANDHRFSKPQWGMQPFNLFAQAFL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LGEDWWHKATTNIRGVNPANEAVVDFSVRQLLDMFAPSNFAVTNPEVVEKTFQTGGENLV
HCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEE
FGWQNWLADLMQVLQPGRTSEKAEFLPGQTVATAPGKIVLRNRLIELIQYAPTTRQVRPE
ECHHHHHHHHHHHHCCCCCCCCHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCC
PILIVPAWIMKYYILDLSHYNSLVRYLTDQGFTVFMISWRNPGARDRDIAFDDYRSTGVM
CEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHH
AALDEIGKIVPNAPVHAAGYCLGGTLLSITAAAMAREGDTRLKTITLFAAQTDFTEAGEL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCE
TLFINESQVAFLEDMMWERGYLDTTQMAGAFQLLRSNDLIWSRVSRDYLLGERAHPSDLM
EEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCE
AWNADATRLPYRMHSEYLRKLFLNNDLAEGRYRVQGKPVSLSDIHTPMFVVGTLRDHVAP
EECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHCCCCHHEEHHHHHHCCC
WKSTYKIHYEVDANVTFVLASGGHNAGIVAPPDEQGHSHQVRTKAADEPYLGPDEWQSTS
CCCEEEEEEEECCCEEEEEECCCCCCCEECCCCCCCCCCEEHHCCCCCCCCCCCCCCCCC
PHIEGSWWPTWISWLAQHSEALTIPPRLGTDDGHELGDAPGEYVHT
CCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDVRLLAQDDVGGEFGREDHELDKAFSAVLARVTGGISPAALSMAYLDWACHLAAAPQR
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHH
QLDNVRRAWDGVTQLAERSLHFADPNRGPWDLIKPQANDHRFSKPQWGMQPFNLFAQAFL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LGEDWWHKATTNIRGVNPANEAVVDFSVRQLLDMFAPSNFAVTNPEVVEKTFQTGGENLV
HCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEE
FGWQNWLADLMQVLQPGRTSEKAEFLPGQTVATAPGKIVLRNRLIELIQYAPTTRQVRPE
ECHHHHHHHHHHHHCCCCCCCCHHCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCC
PILIVPAWIMKYYILDLSHYNSLVRYLTDQGFTVFMISWRNPGARDRDIAFDDYRSTGVM
CEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHH
AALDEIGKIVPNAPVHAAGYCLGGTLLSITAAAMAREGDTRLKTITLFAAQTDFTEAGEL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCE
TLFINESQVAFLEDMMWERGYLDTTQMAGAFQLLRSNDLIWSRVSRDYLLGERAHPSDLM
EEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCE
AWNADATRLPYRMHSEYLRKLFLNNDLAEGRYRVQGKPVSLSDIHTPMFVVGTLRDHVAP
EECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHCCCCHHEEHHHHHHCCC
WKSTYKIHYEVDANVTFVLASGGHNAGIVAPPDEQGHSHQVRTKAADEPYLGPDEWQSTS
CCCEEEEEEEECCCEEEEEECCCCCCCEECCCCCCCCCCEEHHCCCCCCCCCCCCCCCCC
PHIEGSWWPTWISWLAQHSEALTIPPRLGTDDGHELGDAPGEYVHT
CCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438 [H]