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The map label for this gene is fixK [H]

Identifier: 146342150

GI number: 146342150

Start: 5513930

End: 5514514

Strand: Reverse

Name: fixK [H]

Synonym: BRADO5299

Alternate gene names: 146342150

Gene position: 5514514-5513930 (Counterclockwise)

Preceding gene: 146342152

Following gene: 146342149

Centisome position: 73.95

GC content: 60.85

Gene sequence:

>585_bases
GTGAACTTTGCATCCTGCGAGACGGTTTTCGCTCAGGAGGACACGTCGTCTTTCTTTTACAATCTGCTCGACGGAGTCAT
GAGGCTCTACAAGCTCCTCCCCGATGGCCGCAGGCAGATCGTCGGGTTCGTGCTGCCCGGCGATTTCGTCGGCATGACGA
CTGAGGAGCGGCATGGGTTTTCGGCCGATGCGATCGGCCCGGTCACGGTCTGTCAATTTGCCAAGGTGCCGTTTGGCCGG
TTTGCCGAGGACAAGCCAAACCTTCTGCGGCGGATGAATGAACTGGCCGTGCAGGAGCTCAACCAGGCGCGCGATCACAT
GGTGCTGCTGGGCCGCCGCTCAGCCGAAGAGAAGGTTGCAACCTTCCTCGTCGGCTGGAGAGATCGCCTGGTCGAACTGC
GGGGACCCTCTAATATAGTACCGCTGCCGATGAGTCGGCAGGACATCGCTGATTATCTTGGCCTGACCATCGAGACGGTC
AGCCGGACCTTCACCAAACTGGAGCGGGATGGGGTTATTGCGATCCTCCCCGGAGGGGTGTCGCTGCGGGATTCGGCGCG
TGCGGAGGCATTGGCAGCAGCCTGA

Upstream 100 bases:

>100_bases
GCATGGGCAGGGCTGTGCGGATTGTGCCGTACGAGGATTCAGCGTCTGCGCGTCCCTGGATCAGTTCGAGATGCGGGAAT
TCGAGCATCTGAGCCGCCGC

Downstream 100 bases:

>100_bases
TACGGTGCTCGCCTGGGATCCTCGGCCTGCATTGATCCTGGTCAAAGACTGAAGAGCTGCCGCCGGGAACAATGTCTCAA
CTCTAAAAAGGGGAGAGAGA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MNFASCETVFAQEDTSSFFYNLLDGVMRLYKLLPDGRRQIVGFVLPGDFVGMTTEERHGFSADAIGPVTVCQFAKVPFGR
FAEDKPNLLRRMNELAVQELNQARDHMVLLGRRSAEEKVATFLVGWRDRLVELRGPSNIVPLPMSRQDIADYLGLTIETV
SRTFTKLERDGVIAILPGGVSLRDSARAEALAAA

Sequences:

>Translated_194_residues
MNFASCETVFAQEDTSSFFYNLLDGVMRLYKLLPDGRRQIVGFVLPGDFVGMTTEERHGFSADAIGPVTVCQFAKVPFGR
FAEDKPNLLRRMNELAVQELNQARDHMVLLGRRSAEEKVATFLVGWRDRLVELRGPSNIVPLPMSRQDIADYLGLTIETV
SRTFTKLERDGVIAILPGGVSLRDSARAEALAAA
>Mature_194_residues
MNFASCETVFAQEDTSSFFYNLLDGVMRLYKLLPDGRRQIVGFVLPGDFVGMTTEERHGFSADAIGPVTVCQFAKVPFGR
FAEDKPNLLRRMNELAVQELNQARDHMVLLGRRSAEEKVATFLVGWRDRLVELRGPSNIVPLPMSRQDIADYLGLTIETV
SRTFTKLERDGVIAILPGGVSLRDSARAEALAAA

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=189, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 21551; Mature: 21551

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFASCETVFAQEDTSSFFYNLLDGVMRLYKLLPDGRRQIVGFVLPGDFVGMTTEERHGF
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEECCCCHHCCCC
SADAIGPVTVCQFAKVPFGRFAEDKPNLLRRMNELAVQELNQARDHMVLLGRRSAEEKVA
CCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHH
TFLVGWRDRLVELRGPSNIVPLPMSRQDIADYLGLTIETVSRTFTKLERDGVIAILPGGV
HHHHHHHHHEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
SLRDSARAEALAAA
CCCHHHHHHHHHCC
>Mature Secondary Structure
MNFASCETVFAQEDTSSFFYNLLDGVMRLYKLLPDGRRQIVGFVLPGDFVGMTTEERHGF
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEECCCCHHCCCC
SADAIGPVTVCQFAKVPFGRFAEDKPNLLRRMNELAVQELNQARDHMVLLGRRSAEEKVA
CCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHH
TFLVGWRDRLVELRGPSNIVPLPMSRQDIADYLGLTIETVSRTFTKLERDGVIAILPGGV
HHHHHHHHHEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCC
SLRDSARAEALAAA
CCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]