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The map label for this gene is alaS [H]

Identifier: 146342087

GI number: 146342087

Start: 5443797

End: 5444528

Strand: Direct

Name: alaS [H]

Synonym: BRADO5229

Alternate gene names: 146342087

Gene position: 5443797-5444528 (Clockwise)

Preceding gene: 146342086

Following gene: 146342089

Centisome position: 73.01

GC content: 67.9

Gene sequence:

>732_bases
ATGACCAATGTCCTGGCCCGCCCGTTCGCCGTCGATCATCCCGATGTGCTCGAGCTTGATACGACGGCATTGAACGTCCG
CCATGGCGCCGTGCTGCTGTCACGCTCGCCGTTCTATCCCGGAGGCGGCGGCCAATTGCCGGACCGCGGGACCATCGCGT
GGAACGGGATCGTACGCGACGTCACTGGCTTCGAGCCGACGTCGGACGGCCTCTGGCACCAGTTCGGTGACGACGCGGTG
CCGTGGGGGACAATCACGCTCCGCGTCGACGCGGCCTTCCGCCAGCGCATGTGCGAGCAGCACACTGTGGCCCATCTCGT
CAACAGCGCGGTCTACACGCTGTTCGACGGCGCGCTGCTGACCGGCGTGCGCATGACCGACACCGACGAGTTCAGCGTCG
ACTTCGACCTGCCGAAGGTGGCGCCGGAGCAGTTGCGCGCAGTGGAGGACGCGGTGAACGCCGCCATCTCCGACGGCCGC
CCGGTGCGCGCCTTCGACATGCCTTATGACGACGCGCAGGCCACCGCCGGGCTGTTTCGCGCCAAATCTGTTGCCCCGCC
GCGCCAGGCCGACGGCATGATCCGCATCGTCGACATCGACGGCCTCGACCGCCAGGCCTGCGGCGGCACGCATCTGGTGT
CGACGGCGCAGGCGCGGCGCATTCGGATTCTGAAGGTGGACAACAAGGGGCGGCAGAACCGGCGGTTCAGGATCGGGCTG
GGGAGTGATTAG

Upstream 100 bases:

>100_bases
TAGCCCGCATGAGCGCAGCGATATGCGGGTTCTCGCGCACCCTCTCGATGAACCCGGATGTCGCTTCGCTCATCCGGGCT
ACGACAGCAAGCCGTTTTGC

Downstream 100 bases:

>100_bases
CAACGGCCGCGTAGCCCGGATGAGCGCAGCGACATCCGGGGTCTCACGCGCTCTCTCGATGATCCCGGATGTCGCTGTGC
TCATCCGGGCTACCAGGCTG

Product: putative alanyl-tRNA synthetase-like protein

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTNVLARPFAVDHPDVLELDTTALNVRHGAVLLSRSPFYPGGGGQLPDRGTIAWNGIVRDVTGFEPTSDGLWHQFGDDAV
PWGTITLRVDAAFRQRMCEQHTVAHLVNSAVYTLFDGALLTGVRMTDTDEFSVDFDLPKVAPEQLRAVEDAVNAAISDGR
PVRAFDMPYDDAQATAGLFRAKSVAPPRQADGMIRIVDIDGLDRQACGGTHLVSTAQARRIRILKVDNKGRQNRRFRIGL
GSD

Sequences:

>Translated_243_residues
MTNVLARPFAVDHPDVLELDTTALNVRHGAVLLSRSPFYPGGGGQLPDRGTIAWNGIVRDVTGFEPTSDGLWHQFGDDAV
PWGTITLRVDAAFRQRMCEQHTVAHLVNSAVYTLFDGALLTGVRMTDTDEFSVDFDLPKVAPEQLRAVEDAVNAAISDGR
PVRAFDMPYDDAQATAGLFRAKSVAPPRQADGMIRIVDIDGLDRQACGGTHLVSTAQARRIRILKVDNKGRQNRRFRIGL
GSD
>Mature_242_residues
TNVLARPFAVDHPDVLELDTTALNVRHGAVLLSRSPFYPGGGGQLPDRGTIAWNGIVRDVTGFEPTSDGLWHQFGDDAVP
WGTITLRVDAAFRQRMCEQHTVAHLVNSAVYTLFDGALLTGVRMTDTDEFSVDFDLPKVAPEQLRAVEDAVNAAISDGRP
VRAFDMPYDDAQATAGLFRAKSVAPPRQADGMIRIVDIDGLDRQACGGTHLVSTAQARRIRILKVDNKGRQNRRFRIGLG
SD

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=213, Percent_Identity=30.5164319248826, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI217416402, Length=213, Percent_Identity=30.5164319248826, Blast_Score=81, Evalue=7e-16,
Organism=Escherichia coli, GI1789048, Length=204, Percent_Identity=24.5098039215686, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR022429
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 26550; Mature: 26419

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVLARPFAVDHPDVLELDTTALNVRHGAVLLSRSPFYPGGGGQLPDRGTIAWNGIVRD
CCCCCCCCEECCCCCEEEECCEEEEEECCEEEEECCCCCCCCCCCCCCCCEEEECCEEEC
VTGFEPTSDGLWHQFGDDAVPWGTITLRVDAAFRQRMCEQHTVAHLVNSAVYTLFDGALL
CCCCCCCCCCCHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
TGVRMTDTDEFSVDFDLPKVAPEQLRAVEDAVNAAISDGRPVRAFDMPYDDAQATAGLFR
CCEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHH
AKSVAPPRQADGMIRIVDIDGLDRQACGGTHLVSTAQARRIRILKVDNKGRQNRRFRIGL
HCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCEEEEEC
GSD
CCC
>Mature Secondary Structure 
TNVLARPFAVDHPDVLELDTTALNVRHGAVLLSRSPFYPGGGGQLPDRGTIAWNGIVRD
CCCCCCCEECCCCCEEEECCEEEEEECCEEEEECCCCCCCCCCCCCCCCEEEECCEEEC
VTGFEPTSDGLWHQFGDDAVPWGTITLRVDAAFRQRMCEQHTVAHLVNSAVYTLFDGALL
CCCCCCCCCCCHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
TGVRMTDTDEFSVDFDLPKVAPEQLRAVEDAVNAAISDGRPVRAFDMPYDDAQATAGLFR
CCEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHH
AKSVAPPRQADGMIRIVDIDGLDRQACGGTHLVSTAQARRIRILKVDNKGRQNRRFRIGL
HCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCEEEEEC
GSD
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA