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The map label for this gene is pcpB [H]

Identifier: 146342083

GI number: 146342083

Start: 5438612

End: 5440540

Strand: Reverse

Name: pcpB [H]

Synonym: BRADO5224

Alternate gene names: 146342083

Gene position: 5440540-5438612 (Counterclockwise)

Preceding gene: 146342085

Following gene: 146342082

Centisome position: 72.96

GC content: 67.08

Gene sequence:

>1929_bases
GTGCAATTTCATCTCAACGGCTTTCAGCCGGGCGACCCTGAGATTTTCGATACGGCGGAGCGCGTGGCAAGCTCCGGCGC
GGCAGGCGCGCTGCCCTCGGAGGTCGACGTCCTCATCGTCGGCTGCGGCCCGGCGGGATTGAATCTCGCGGCCCAGCTGT
CGGCTTTTCCGGACATCACAACCGCTATCGTCGAGCAGAAGCCGGGCCGCCTGCTGCTCGGCCAGGCCGACGGCATCGCC
TGCCGGACGATGGAGATGTTCCAGGCCTACGGCTTCGCCGAGAGGGTGCTGAAGGAGGCCTGCTGGATCAACGAGACCAC
GTTCTGGAAGCCCGCTGAGGCGAACACCGCGCAGATCGCGCGCAGCGGCCGCGTGCAGGACGTCGAGGATGGCCTGTCCG
AGATGCCGCATGTCGTGCTCAACCAGGCCCGCGTGCACGATTTTTTCCTCGACGTCATGCGACACTCGGCGGCGAAGATG
CAGCCGTACTACGCGCGGCGCCTGCGCGATCTCACGATTGATGAAGCCGCGCCGGCCGGCGCGCACGGTGTCAGCGTGAG
CTTCGACCGCATCGATGCCGCGCATGAGGGGCAGGTCGAAACGGTGCGCGCGCGCTACGTCGTCGGCTGCGACGGCGCCC
GCAGCGTGGTGCGCAAGGCGATCGGCCGCGAGCTGCATGGCGATTCCGCCAACCACGCCTGGGGCGTGATGGACGTGCTC
GCGGTCACCGATTTCCCCGACATCCGTTTCAAGTCGCTGATCCAGTCGCCCAAGGACGGCAGCCTGCTGGTGATCCCGCG
CGAGGGCGGCTATCTGGTGCGGCTGTATGTCGAGCTCGCCAAGCTCGAGGTCGGCGAGCGTGTCGCCGGCCGCAACATCA
CCGTCGAGGAGGTGATCGCCAAGGCGCAGCGCATTCTCGCGCCTTATACGCTGGAGGTGAAGGAGGTCGCGTGGTGGTCG
GTCTACGAGATCGGGCAGCGCATCACCGACAAGTTCGACGACGTGCCGGAGGCGGAGGTCGCCACAAGGCTGCCGCGCGT
GTTCATCGCCGGCGACGCCTGCCACACCCACAGCCCCAAGGCGGGGCAGGGCATGAACGTGTCGATGCAGGACACCTTCA
ATCTCGGCTGGAAGCTCGCCGCCGTGCTGGGCAAGCGCTGCGCGCCCGAGCTGCTGCACAGCTACTCGGCCGAACGCAAG
GCGATCGCGCAGGAGCTGATCGATTTCGATCGTGAATGGTCGGGGATCCTCGCCTCCGCCGCCAAGCAGGGCGGTGCGGA
TGCGGCCAAGACGCAGGACTATTTCGTCCGCCACGGCCGCTACACCGCCGGTACCGCCACGCAGTACCGGCCCTCGCTGC
TGACCGGCGACGACACCTTCCAGCAGCTCGCCGCCGGCTTCGTCATCGGCAAGCGGTTCCACTCAGCACCCGTGATTCGC
CTCGGCGACGCCAAGCGGGTGCAACTCGGTCACGTGGTCCAGGCCGACGGCCGCTTCCGCCTGTTCGCCTTCGCCGGCGC
CGAGGACCCCACGGCTCAAGTGTCCGCCATCCGCCGCCTGTGTGATTTCCTCAGTGACAACGCGGCCTCGCCGCTGCGGC
GCTACACGCCTGCCGGCGCCGACATCGATTCCGTGATCGACCTTCGCGCCGTATTCCAGCAGGGCCATCGCGAGCTCGCT
CTGGAGGAGATGCCGTCGCTGCTGCTGCCGCGGAAGGGCCGCTACGGCCTCGTCGATTACGAGAAGATGTTCTGCTCCGA
TCTGAAGAGCGGGCAGGACATCTTTGCGATGCGCGGCATCGATCGTGAGGGCGGCTGCCTCGTGATCGTGCGGCCTGACC
AGTATGTCGCCGACATCATGCCGCTCGACGATGTCGGGCGGCTGTCGGCCTATTTCGACGGTTTCTTGCTGGCGGCCGGC
TCAAGGTAA

Upstream 100 bases:

>100_bases
TCCTCCATTTCGGTCACCACGAGTCTCCTTCGGTCTGCTTGACAAGCATACTGATGATCAGCATACTGATCAATAAGGAA
GCGCAAGGGAGGGTGGTCAC

Downstream 100 bases:

>100_bases
ACGAAATCCGAATCGCAGCCCCGGAATCCCCGACGTCACGCTAGCCAGATTCCAATGTTTCGCCGTTACAGATGATCGTC
CAGGATGGACGATTGAGTGG

Product: phenol 2-monooxygenase

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 642; Mature: 642

Protein sequence:

>642_residues
MQFHLNGFQPGDPEIFDTAERVASSGAAGALPSEVDVLIVGCGPAGLNLAAQLSAFPDITTAIVEQKPGRLLLGQADGIA
CRTMEMFQAYGFAERVLKEACWINETTFWKPAEANTAQIARSGRVQDVEDGLSEMPHVVLNQARVHDFFLDVMRHSAAKM
QPYYARRLRDLTIDEAAPAGAHGVSVSFDRIDAAHEGQVETVRARYVVGCDGARSVVRKAIGRELHGDSANHAWGVMDVL
AVTDFPDIRFKSLIQSPKDGSLLVIPREGGYLVRLYVELAKLEVGERVAGRNITVEEVIAKAQRILAPYTLEVKEVAWWS
VYEIGQRITDKFDDVPEAEVATRLPRVFIAGDACHTHSPKAGQGMNVSMQDTFNLGWKLAAVLGKRCAPELLHSYSAERK
AIAQELIDFDREWSGILASAAKQGGADAAKTQDYFVRHGRYTAGTATQYRPSLLTGDDTFQQLAAGFVIGKRFHSAPVIR
LGDAKRVQLGHVVQADGRFRLFAFAGAEDPTAQVSAIRRLCDFLSDNAASPLRRYTPAGADIDSVIDLRAVFQQGHRELA
LEEMPSLLLPRKGRYGLVDYEKMFCSDLKSGQDIFAMRGIDREGGCLVIVRPDQYVADIMPLDDVGRLSAYFDGFLLAAG
SR

Sequences:

>Translated_642_residues
MQFHLNGFQPGDPEIFDTAERVASSGAAGALPSEVDVLIVGCGPAGLNLAAQLSAFPDITTAIVEQKPGRLLLGQADGIA
CRTMEMFQAYGFAERVLKEACWINETTFWKPAEANTAQIARSGRVQDVEDGLSEMPHVVLNQARVHDFFLDVMRHSAAKM
QPYYARRLRDLTIDEAAPAGAHGVSVSFDRIDAAHEGQVETVRARYVVGCDGARSVVRKAIGRELHGDSANHAWGVMDVL
AVTDFPDIRFKSLIQSPKDGSLLVIPREGGYLVRLYVELAKLEVGERVAGRNITVEEVIAKAQRILAPYTLEVKEVAWWS
VYEIGQRITDKFDDVPEAEVATRLPRVFIAGDACHTHSPKAGQGMNVSMQDTFNLGWKLAAVLGKRCAPELLHSYSAERK
AIAQELIDFDREWSGILASAAKQGGADAAKTQDYFVRHGRYTAGTATQYRPSLLTGDDTFQQLAAGFVIGKRFHSAPVIR
LGDAKRVQLGHVVQADGRFRLFAFAGAEDPTAQVSAIRRLCDFLSDNAASPLRRYTPAGADIDSVIDLRAVFQQGHRELA
LEEMPSLLLPRKGRYGLVDYEKMFCSDLKSGQDIFAMRGIDREGGCLVIVRPDQYVADIMPLDDVGRLSAYFDGFLLAAG
SR
>Mature_642_residues
MQFHLNGFQPGDPEIFDTAERVASSGAAGALPSEVDVLIVGCGPAGLNLAAQLSAFPDITTAIVEQKPGRLLLGQADGIA
CRTMEMFQAYGFAERVLKEACWINETTFWKPAEANTAQIARSGRVQDVEDGLSEMPHVVLNQARVHDFFLDVMRHSAAKM
QPYYARRLRDLTIDEAAPAGAHGVSVSFDRIDAAHEGQVETVRARYVVGCDGARSVVRKAIGRELHGDSANHAWGVMDVL
AVTDFPDIRFKSLIQSPKDGSLLVIPREGGYLVRLYVELAKLEVGERVAGRNITVEEVIAKAQRILAPYTLEVKEVAWWS
VYEIGQRITDKFDDVPEAEVATRLPRVFIAGDACHTHSPKAGQGMNVSMQDTFNLGWKLAAVLGKRCAPELLHSYSAERK
AIAQELIDFDREWSGILASAAKQGGADAAKTQDYFVRHGRYTAGTATQYRPSLLTGDDTFQQLAAGFVIGKRFHSAPVIR
LGDAKRVQLGHVVQADGRFRLFAFAGAEDPTAQVSAIRRLCDFLSDNAASPLRRYTPAGADIDSVIDLRAVFQQGHRELA
LEEMPSLLLPRKGRYGLVDYEKMFCSDLKSGQDIFAMRGIDREGGCLVIVRPDQYVADIMPLDDVGRLSAYFDGFLLAAG
SR

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 70329; Mature: 70329

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFHLNGFQPGDPEIFDTAERVASSGAAGALPSEVDVLIVGCGPAGLNLAAQLSAFPDIT
CEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCHH
TAIVEQKPGRLLLGQADGIACRTMEMFQAYGFAERVLKEACWINETTFWKPAEANTAQIA
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
RSGRVQDVEDGLSEMPHVVLNQARVHDFFLDVMRHSAAKMQPYYARRLRDLTIDEAAPAG
HCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCC
AHGVSVSFDRIDAAHEGQVETVRARYVVGCDGARSVVRKAIGRELHGDSANHAWGVMDVL
CCCCEEEHHHCCCCCCCCCEEEEEEEEEECCHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AVTDFPDIRFKSLIQSPKDGSLLVIPREGGYLVRLYVELAKLEVGERVAGRNITVEEVIA
HHCCCCCHHHHHHHCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHH
KAQRILAPYTLEVKEVAWWSVYEIGQRITDKFDDVPEAEVATRLPRVFIAGDACHTHSPK
HHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCEEEEECCCCCCCCCC
AGQGMNVSMQDTFNLGWKLAAVLGKRCAPELLHSYSAERKAIAQELIDFDREWSGILASA
CCCCCCEEHHHHHCCHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHH
AKQGGADAAKTQDYFVRHGRYTAGTATQYRPSLLTGDDTFQQLAAGFVIGKRFHSAPVIR
HHCCCCCCHHHHHHHHHCCCEECCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCEEE
LGDAKRVQLGHVVQADGRFRLFAFAGAEDPTAQVSAIRRLCDFLSDNAASPLRRYTPAGA
ECCCCEEEECCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCC
DIDSVIDLRAVFQQGHRELALEEMPSLLLPRKGRYGLVDYEKMFCSDLKSGQDIFAMRGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCC
DREGGCLVIVRPDQYVADIMPLDDVGRLSAYFDGFLLAAGSR
CCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHCCEEEECCCC
>Mature Secondary Structure
MQFHLNGFQPGDPEIFDTAERVASSGAAGALPSEVDVLIVGCGPAGLNLAAQLSAFPDIT
CEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCHH
TAIVEQKPGRLLLGQADGIACRTMEMFQAYGFAERVLKEACWINETTFWKPAEANTAQIA
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
RSGRVQDVEDGLSEMPHVVLNQARVHDFFLDVMRHSAAKMQPYYARRLRDLTIDEAAPAG
HCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCC
AHGVSVSFDRIDAAHEGQVETVRARYVVGCDGARSVVRKAIGRELHGDSANHAWGVMDVL
CCCCEEEHHHCCCCCCCCCEEEEEEEEEECCHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AVTDFPDIRFKSLIQSPKDGSLLVIPREGGYLVRLYVELAKLEVGERVAGRNITVEEVIA
HHCCCCCHHHHHHHCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHH
KAQRILAPYTLEVKEVAWWSVYEIGQRITDKFDDVPEAEVATRLPRVFIAGDACHTHSPK
HHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCEEEEECCCCCCCCCC
AGQGMNVSMQDTFNLGWKLAAVLGKRCAPELLHSYSAERKAIAQELIDFDREWSGILASA
CCCCCCEEHHHHHCCHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHH
AKQGGADAAKTQDYFVRHGRYTAGTATQYRPSLLTGDDTFQQLAAGFVIGKRFHSAPVIR
HHCCCCCCHHHHHHHHHCCCEECCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCEEE
LGDAKRVQLGHVVQADGRFRLFAFAGAEDPTAQVSAIRRLCDFLSDNAASPLRRYTPAGA
ECCCCEEEECCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCC
DIDSVIDLRAVFQQGHRELALEEMPSLLLPRKGRYGLVDYEKMFCSDLKSGQDIFAMRGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCC
DREGGCLVIVRPDQYVADIMPLDDVGRLSAYFDGFLLAAGSR
CCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]