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The map label for this gene is algI [H]

Identifier: 146342024

GI number: 146342024

Start: 5371405

End: 5372910

Strand: Direct

Name: algI [H]

Synonym: BRADO5161

Alternate gene names: 146342024

Gene position: 5371405-5372910 (Clockwise)

Preceding gene: 146342022

Following gene: 146342025

Centisome position: 72.04

GC content: 59.43

Gene sequence:

>1506_bases
ATGCTCTTCACCCAATTCGAGTTCATCTTCCTGTTCTTGCCGGTGACCTTCGCCGGCTATTTTCTTATCGGGCGTCGTTT
TGCCGATCCGACTCCGCGTCTCGTCTGGCTGGCTGCGGCCTCGCTCGTGTTCTATGCCAATTGGGACATCGGCTTTATTC
CGATCATCTTGACGTCAATCGTCTTCAATTATGCCCTTGGACTTCTGATCGCCGCTCAGACATCCAAGCCCGTGCGAACC
AGGCTGTTCGCCGTCGCAGTGGTCGCAAACCTTCTCGCTCTCGCTTTCTTCAAGTACACGAATTTCGGCATCGACATCTT
CGATGCAATCATGCGGAACCGGCATCCACATCTCGACATCGCGCTGCCGCTCGGGATCTCGTTCTTCACCTTCACGCAGC
TGGCCTATCTTGCTGACGTCTACGCCGGCGTCGCAACCGAGCGCAGCTTCGTCAAATACACCCTGTTCGTAACCTATTTC
CCGCATCTGATCGCAGGACCAATCTTGCATCACCGGGAGATGATGCCGCAATTCGGCTCGGATGAAAGCAAGAGCGTTTC
GGTCGAGCGCGTCGCCACCGGGATCACCATCTTCATCATCGGCTTGTTCAAGAAGGCGGTGATCGCGGACGGGTTCGCGC
TGATCGCAAATCCCGTTTTCCAGACCGCAAGCCGGTTTCATCTTCACGCCATCGATGCCTGGGGCGGTGCGCTGGCCTAC
TCGCTGCAGATCTATTTCGACTTCTCCGGCTATTCGGACATGGCGATCGGACTGTCGCTGCTGTTCGGTATCCGTCTTCC
GTTCAATTTCGATGCGCCCTACAAATCCCGCAGCATCATCGAATTCTGGCGCCGCTGGCACATCACCCTGTCGCGCTTCC
TCAGAGATTATCTGTACATCCCTCTCGGCGGCAACCGACACGGCAGCAGCCGGCGCCGCCTCAACCTGTTTCTGACCATG
GTGATCGGTGGCCTCTGGCACGGCGCAGGCTTCACCTTCGTGATTTGGGGCGCGCTTCACGGTGGCTACCTGATCGTCAA
TCACGTGTGGCGGGAGACCGTCGGTCGCTGGCCAGCGCTGCAGCGGCACTTGAACAGCTTCTGGTACCTCGCATCTACGC
TGGTGCTGACGCAGCTGGCTGTGGTGATCGCATGGGTGTTCTTCCGCGCCGAGACTCCCAGGATCGCCGGTCGTGTGCTC
CGCGCCATGGTTGGAGCCTCGCTGAACGATCAGATCGCACCCAGTAACACGGTGACCTGGCTGTCGCTCGCGGCCGTGAT
CGCCGGCTACGCCGCCTGCCTGCTGCTCACGAACGTCAACGCCATGTTCGATGTCTGGAACGTCGGCTTGAAGAGCTACG
ACAATCCGCGGGGCTGGAGCCTCGTCGAATTGCAATGGCGACCGACCACCGGCTGGGCCGTTGCGGCATCCCTGGTCTGC
TCGGCAGCGCTGCTCGTGAGCCTGCTCGCCGGCGACGGAGCGCAGTTTCTCTATTTCCAGTTCTGA

Upstream 100 bases:

>100_bases
CGCCTTCACCGGGTCCATGTCCGGGCAACGGGGGGCTAGCTTGGCAGGCCCGGTCAGAGTTGATACGATCCCCGGCTCAG
TGCTGCCTTGGCCTTTCGAC

Downstream 100 bases:

>100_bases
AATCCGCCGGAGCACGACGTGTTGGAACATCGAAGAGCTGACGACGGCACGTCGGAGACCGCGGACGCGTCACGCCGGCC
CGAGGAGCTGAAATCCTCCG

Product: putative membrane bound O-acyl transferase (MBOAT) family protein alginate O-acetyltransferase

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MLFTQFEFIFLFLPVTFAGYFLIGRRFADPTPRLVWLAAASLVFYANWDIGFIPIILTSIVFNYALGLLIAAQTSKPVRT
RLFAVAVVANLLALAFFKYTNFGIDIFDAIMRNRHPHLDIALPLGISFFTFTQLAYLADVYAGVATERSFVKYTLFVTYF
PHLIAGPILHHREMMPQFGSDESKSVSVERVATGITIFIIGLFKKAVIADGFALIANPVFQTASRFHLHAIDAWGGALAY
SLQIYFDFSGYSDMAIGLSLLFGIRLPFNFDAPYKSRSIIEFWRRWHITLSRFLRDYLYIPLGGNRHGSSRRRLNLFLTM
VIGGLWHGAGFTFVIWGALHGGYLIVNHVWRETVGRWPALQRHLNSFWYLASTLVLTQLAVVIAWVFFRAETPRIAGRVL
RAMVGASLNDQIAPSNTVTWLSLAAVIAGYAACLLLTNVNAMFDVWNVGLKSYDNPRGWSLVELQWRPTTGWAVAASLVC
SAALLVSLLAGDGAQFLYFQF

Sequences:

>Translated_501_residues
MLFTQFEFIFLFLPVTFAGYFLIGRRFADPTPRLVWLAAASLVFYANWDIGFIPIILTSIVFNYALGLLIAAQTSKPVRT
RLFAVAVVANLLALAFFKYTNFGIDIFDAIMRNRHPHLDIALPLGISFFTFTQLAYLADVYAGVATERSFVKYTLFVTYF
PHLIAGPILHHREMMPQFGSDESKSVSVERVATGITIFIIGLFKKAVIADGFALIANPVFQTASRFHLHAIDAWGGALAY
SLQIYFDFSGYSDMAIGLSLLFGIRLPFNFDAPYKSRSIIEFWRRWHITLSRFLRDYLYIPLGGNRHGSSRRRLNLFLTM
VIGGLWHGAGFTFVIWGALHGGYLIVNHVWRETVGRWPALQRHLNSFWYLASTLVLTQLAVVIAWVFFRAETPRIAGRVL
RAMVGASLNDQIAPSNTVTWLSLAAVIAGYAACLLLTNVNAMFDVWNVGLKSYDNPRGWSLVELQWRPTTGWAVAASLVC
SAALLVSLLAGDGAQFLYFQF
>Mature_501_residues
MLFTQFEFIFLFLPVTFAGYFLIGRRFADPTPRLVWLAAASLVFYANWDIGFIPIILTSIVFNYALGLLIAAQTSKPVRT
RLFAVAVVANLLALAFFKYTNFGIDIFDAIMRNRHPHLDIALPLGISFFTFTQLAYLADVYAGVATERSFVKYTLFVTYF
PHLIAGPILHHREMMPQFGSDESKSVSVERVATGITIFIIGLFKKAVIADGFALIANPVFQTASRFHLHAIDAWGGALAY
SLQIYFDFSGYSDMAIGLSLLFGIRLPFNFDAPYKSRSIIEFWRRWHITLSRFLRDYLYIPLGGNRHGSSRRRLNLFLTM
VIGGLWHGAGFTFVIWGALHGGYLIVNHVWRETVGRWPALQRHLNSFWYLASTLVLTQLAVVIAWVFFRAETPRIAGRVL
RAMVGASLNDQIAPSNTVTWLSLAAVIAGYAACLLLTNVNAMFDVWNVGLKSYDNPRGWSLVELQWRPTTGWAVAASLVC
SAALLVSLLAGDGAQFLYFQF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 56256; Mature: 56256

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFTQFEFIFLFLPVTFAGYFLIGRRFADPTPRLVWLAAASLVFYANWDIGFIPIILTSI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHH
VFNYALGLLIAAQTSKPVRTRLFAVAVVANLLALAFFKYTNFGIDIFDAIMRNRHPHLDI
HHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEE
ALPLGISFFTFTQLAYLADVYAGVATERSFVKYTLFVTYFPHLIAGPILHHREMMPQFGS
EECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DESKSVSVERVATGITIFIIGLFKKAVIADGFALIANPVFQTASRFHLHAIDAWGGALAY
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHEEEEEECCCCEEEE
SLQIYFDFSGYSDMAIGLSLLFGIRLPFNFDAPYKSRSIIEFWRRWHITLSRFLRDYLYI
EEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEE
PLGGNRHGSSRRRLNLFLTMVIGGLWHGAGFTFVIWGALHGGYLIVNHVWRETVGRWPAL
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEEEHHCCCCHHHHHHHHHHHCCCHHH
QRHLNSFWYLASTLVLTQLAVVIAWVFFRAETPRIAGRVLRAMVGASLNDQIAPSNTVTW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHH
LSLAAVIAGYAACLLLTNVNAMFDVWNVGLKSYDNPRGWSLVELQWRPTTGWAVAASLVC
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHH
SAALLVSLLAGDGAQFLYFQF
HHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MLFTQFEFIFLFLPVTFAGYFLIGRRFADPTPRLVWLAAASLVFYANWDIGFIPIILTSI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHH
VFNYALGLLIAAQTSKPVRTRLFAVAVVANLLALAFFKYTNFGIDIFDAIMRNRHPHLDI
HHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEE
ALPLGISFFTFTQLAYLADVYAGVATERSFVKYTLFVTYFPHLIAGPILHHREMMPQFGS
EECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DESKSVSVERVATGITIFIIGLFKKAVIADGFALIANPVFQTASRFHLHAIDAWGGALAY
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHEEEEEECCCCEEEE
SLQIYFDFSGYSDMAIGLSLLFGIRLPFNFDAPYKSRSIIEFWRRWHITLSRFLRDYLYI
EEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEE
PLGGNRHGSSRRRLNLFLTMVIGGLWHGAGFTFVIWGALHGGYLIVNHVWRETVGRWPAL
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEEEHHCCCCHHHHHHHHHHHCCCHHH
QRHLNSFWYLASTLVLTQLAVVIAWVFFRAETPRIAGRVLRAMVGASLNDQIAPSNTVTW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHH
LSLAAVIAGYAACLLLTNVNAMFDVWNVGLKSYDNPRGWSLVELQWRPTTGWAVAASLVC
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHH
SAALLVSLLAGDGAQFLYFQF
HHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]