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The map label for this gene is thiD [H]

Identifier: 146341997

GI number: 146341997

Start: 5331475

End: 5332275

Strand: Reverse

Name: thiD [H]

Synonym: BRADO5133

Alternate gene names: 146341997

Gene position: 5332275-5331475 (Counterclockwise)

Preceding gene: 146342000

Following gene: 146341996

Centisome position: 71.51

GC content: 70.16

Gene sequence:

>801_bases
ATGAGCACGCCCATAGCCCTCACCATCGCCGGCTCGGATTCCTCGGGAGGAGCCGGCATTCAGGCCGATCTGAAGACCTT
CGCCGCGCTGTCGGTTTATGGTGCCTCGGTGCTGACGGCGCTGACCGCGCAGAACACCCGGGGTGTCACCGGCGTGCATC
TGGTGCCGCCCGATTTCGTCGCCGCGCAGATCGACGCCGTGTTCGACGATCTCGCCGTCGGCGCGGTCAAGATCGGCATG
GTGGCGCAGGGGCCGATTGCCGACGCCATCGCCGCCAGCCTGGCGCGCTGGCGCCCCGCGCACATCGTGCTCGATCCGGT
GATGGTGGCAACCTCCGGGGCGCGGCTGTTGCCGGAGGACGCGATCGAGGCGCTGCGCAGCCGGCTCATCCCGCAGGCGT
CGCTGATCACGCCAAACCTGCCGGAAGCGGCCGCGCTGCTCGGCGAGCCGGTTGCCGAGACCGACGAAGCGATTGCCGCG
CAGGGCCGCCGCTTGCTCGCGCTCGGCTGTCCGGCGGTGCTGATCAAGGGCGGACATGGGCAGGGCGCCGAAAGCACCGA
TCACCTGATCACGTCCGATAGGAGCATCCCGCTGCCGGCGCCGCGCGTCGGCACGAAGAACACGCACGGCACCGGCTGCT
CGCTGTCATCGGCGGTCGCCGCGGGGCTCGCGAAGGGCGAGGATCTGGAGACCGCCGTGCGCAACGCCAAGGCCTTCATC
TCCGGCGCGATTGCGGCCGCCGACCGCTTCACGGTGGGCCGCGGCCACGGCCCGGTGCATCATCTCTATCGGTTCTATTG
A

Upstream 100 bases:

>100_bases
GTTTCAAGCCTCAATATAGCGTTTTCGGGCGAAGTCGAGCCGCATTCTGGGGGCCCGGGTCTGCTTGCCGGGCGCGGCCG
CCCCGCGCTAGGAATGGCGG

Downstream 100 bases:

>100_bases
GGTCACACGGTTTTGAGGCCGCGGCGCGCGGTCGATCCGCGTGAGAGTGGCCGGTCATGCTAGGTGAGACGTGCCGTCGA
TCATCACGGGCGGCAATTGG

Product: bifunctional hydroxy-methylpyrimidine kinase (HMP kinase)/hydroxy-phosphomethylpyrimidine kinase (HMP-P kinase)

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSTPIALTIAGSDSSGGAGIQADLKTFAALSVYGASVLTALTAQNTRGVTGVHLVPPDFVAAQIDAVFDDLAVGAVKIGM
VAQGPIADAIAASLARWRPAHIVLDPVMVATSGARLLPEDAIEALRSRLIPQASLITPNLPEAAALLGEPVAETDEAIAA
QGRRLLALGCPAVLIKGGHGQGAESTDHLITSDRSIPLPAPRVGTKNTHGTGCSLSSAVAAGLAKGEDLETAVRNAKAFI
SGAIAAADRFTVGRGHGPVHHLYRFY

Sequences:

>Translated_266_residues
MSTPIALTIAGSDSSGGAGIQADLKTFAALSVYGASVLTALTAQNTRGVTGVHLVPPDFVAAQIDAVFDDLAVGAVKIGM
VAQGPIADAIAASLARWRPAHIVLDPVMVATSGARLLPEDAIEALRSRLIPQASLITPNLPEAAALLGEPVAETDEAIAA
QGRRLLALGCPAVLIKGGHGQGAESTDHLITSDRSIPLPAPRVGTKNTHGTGCSLSSAVAAGLAKGEDLETAVRNAKAFI
SGAIAAADRFTVGRGHGPVHHLYRFY
>Mature_265_residues
STPIALTIAGSDSSGGAGIQADLKTFAALSVYGASVLTALTAQNTRGVTGVHLVPPDFVAAQIDAVFDDLAVGAVKIGMV
AQGPIADAIAASLARWRPAHIVLDPVMVATSGARLLPEDAIEALRSRLIPQASLITPNLPEAAALLGEPVAETDEAIAAQ
GRRLLALGCPAVLIKGGHGQGAESTDHLITSDRSIPLPAPRVGTKNTHGTGCSLSSAVAAGLAKGEDLETAVRNAKAFIS
GAIAAADRFTVGRGHGPVHHLYRFY

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=261, Percent_Identity=55.5555555555556, Blast_Score=283, Evalue=6e-78,
Organism=Saccharomyces cerevisiae, GI6325378, Length=276, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6324997, Length=273, Percent_Identity=34.0659340659341, Blast_Score=126, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324517, Length=273, Percent_Identity=33.6996336996337, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27023; Mature: 26892

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPIALTIAGSDSSGGAGIQADLKTFAALSVYGASVLTALTAQNTRGVTGVHLVPPDFV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHH
AAQIDAVFDDLAVGAVKIGMVAQGPIADAIAASLARWRPAHIVLDPVMVATSGARLLPED
HHHHHHHHHHHHHHHHEECEEECCCHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCCHH
AIEALRSRLIPQASLITPNLPEAAALLGEPVAETDEAIAAQGRRLLALGCPAVLIKGGHG
HHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCEEEEECCCEEEEECCCC
QGAESTDHLITSDRSIPLPAPRVGTKNTHGTGCSLSSAVAAGLAKGEDLETAVRNAKAFI
CCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
SGAIAAADRFTVGRGHGPVHHLYRFY
HHHHHHHCCEEECCCCCHHHHHHHCC
>Mature Secondary Structure 
STPIALTIAGSDSSGGAGIQADLKTFAALSVYGASVLTALTAQNTRGVTGVHLVPPDFV
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHH
AAQIDAVFDDLAVGAVKIGMVAQGPIADAIAASLARWRPAHIVLDPVMVATSGARLLPED
HHHHHHHHHHHHHHHHEECEEECCCHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCCHH
AIEALRSRLIPQASLITPNLPEAAALLGEPVAETDEAIAAQGRRLLALGCPAVLIKGGHG
HHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCEEEEECCCEEEEECCCC
QGAESTDHLITSDRSIPLPAPRVGTKNTHGTGCSLSSAVAAGLAKGEDLETAVRNAKAFI
CCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
SGAIAAADRFTVGRGHGPVHHLYRFY
HHHHHHHCCEEECCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]