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The map label for this gene is ttuE [H]

Identifier: 146341957

GI number: 146341957

Start: 5292063

End: 5293481

Strand: Reverse

Name: ttuE [H]

Synonym: BRADO5093

Alternate gene names: 146341957

Gene position: 5293481-5292063 (Counterclockwise)

Preceding gene: 146341958

Following gene: 146341956

Centisome position: 70.99

GC content: 65.05

Gene sequence:

>1419_bases
ATGCGTCGTCACCGTCGAGCCAAGATCGTCGCGACCGTCGGTCCCGCCAGCAATTCTCCTGACATGCTGAAGGAGCTGTT
CCTCGCCGGTGTCGATACGTTCCGTCTCAACTTCAGCCACGGCACGCAGGCTGATCACGCCAAGGTTCATGCCGCGATCC
GCGCGCTGGAAAAGGACGTGCAGCGTCCGATCGGCATCCTGATGGATCTGCAGGGACCGAAGATCCGCGTCGGTACCCTG
CGCGACAAGAAGATCCAGGTCGAAGCGGGCGAGACGATTCGATTTGTGTTGAATGGGACCGAGGGTGACAAGACGTCGAT
TCCGTTGCCGCATCCGGAGATCTTTGCGGCGGTCGCGCCCGGTCACGATCTTCTGATCGACGACGGCCGCGTGCGCGTGC
GTGTCACCGGGCTCGGCGACGACTGGATCGAAGCCAAGGTGATCGTCAGCGGCGCGATCTCCAATCACAAGGGCGTCAAC
CTGCCCGGCACGATCCTCGAACTGTCGCCGCTGACCGCGAAGGATCGTTCCGATCTTACGTTCGGCCTCGACCTCGGCGT
CGACTGGGTCGCGCTGTCCTTCGTGCAGAAGCCATCCGACGTGATCGAGGCGAGGGGGCTCATCGACGGCCGCGCCGGCA
TCATGTCCAAGATCGAGAAGCCTGCCGCGCTCGACCGCATCGACGACATCATCCAGCTGTCGGATGCGATCATGGTGGCG
CGCGGGGATCTCGGTGTCGAAATTCCGAATGAGGATGTGCCGGGCAAGCAGAAGGAGCTGGTGCGCGCCTGCCGGCTCGC
GGTGAAGCCGGTGATCGTCGCGACGCAGATGCTGGACTCGATGGTCGCCACACCCACGCCGACGCGCGCGGAAGTCTCCG
ACGTCGCGACCGCGATCTATGACGGCGCCGATGCGGTGATGCTGTCGGCGGAATCGGCCAGCGGCCAGTATCCGCGCGAG
GCCGTGGCGATGATGGACAGCATCATCAAGTCGACCGAGAAGCACAAGATGTACGCCTCGATTATCCAGGCGACGCAGCC
CGACGAGGAGCAGACGCCGCCGCATGCGGTGGCGACCGCCGCGGCCGATCTCGCCTCGGCGATCCATGCCAAGGCGATCG
TGGCCTACACGTCGAGCGGTACCTCGGCCTCGCGCGTCGCGCGCAAGCGGCCGAGCCTGCCGATCCTTGCGATCACGCCG
AACGAGGACACCTCGCGGCGGATGTGCCTGTTGTGGGGCGCGCACAGCGTGCTGTCGCAGGACGTGCAGAGCTATGAGGA
GATGGTCGAGCGCGCGACGTTTTTCGCGGTCCAGGAGGAGTTCGCCGACAAGGGCGATCTGCTGGTCGTCGTCGCCGGCA
TCCCGTTCGCGCAGGCCGGCACCACCAACAATCTACGCGTCGTCACCGTGCCCAGATAG

Upstream 100 bases:

>100_bases
CGCCGCGGGGCGTATACAGCCGCTTCCGCTGCGTCCACGCATGCTCAAGGCATGCGCGTCATAGAGACGCGATATCTGGG
TTGGGGAGAAAGGACACATC

Downstream 100 bases:

>100_bases
GAGGCAGCCGAATTCCCTGTATGCGCCGCCCGCTTGTCGCCTGATTGTCGCACCCCGCTGGTATCGCGGGGCAGTGGCGA
GGACATCGTGAGTCGTCATG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MRRHRRAKIVATVGPASNSPDMLKELFLAGVDTFRLNFSHGTQADHAKVHAAIRALEKDVQRPIGILMDLQGPKIRVGTL
RDKKIQVEAGETIRFVLNGTEGDKTSIPLPHPEIFAAVAPGHDLLIDDGRVRVRVTGLGDDWIEAKVIVSGAISNHKGVN
LPGTILELSPLTAKDRSDLTFGLDLGVDWVALSFVQKPSDVIEARGLIDGRAGIMSKIEKPAALDRIDDIIQLSDAIMVA
RGDLGVEIPNEDVPGKQKELVRACRLAVKPVIVATQMLDSMVATPTPTRAEVSDVATAIYDGADAVMLSAESASGQYPRE
AVAMMDSIIKSTEKHKMYASIIQATQPDEEQTPPHAVATAAADLASAIHAKAIVAYTSSGTSASRVARKRPSLPILAITP
NEDTSRRMCLLWGAHSVLSQDVQSYEEMVERATFFAVQEEFADKGDLLVVVAGIPFAQAGTTNNLRVVTVPR

Sequences:

>Translated_472_residues
MRRHRRAKIVATVGPASNSPDMLKELFLAGVDTFRLNFSHGTQADHAKVHAAIRALEKDVQRPIGILMDLQGPKIRVGTL
RDKKIQVEAGETIRFVLNGTEGDKTSIPLPHPEIFAAVAPGHDLLIDDGRVRVRVTGLGDDWIEAKVIVSGAISNHKGVN
LPGTILELSPLTAKDRSDLTFGLDLGVDWVALSFVQKPSDVIEARGLIDGRAGIMSKIEKPAALDRIDDIIQLSDAIMVA
RGDLGVEIPNEDVPGKQKELVRACRLAVKPVIVATQMLDSMVATPTPTRAEVSDVATAIYDGADAVMLSAESASGQYPRE
AVAMMDSIIKSTEKHKMYASIIQATQPDEEQTPPHAVATAAADLASAIHAKAIVAYTSSGTSASRVARKRPSLPILAITP
NEDTSRRMCLLWGAHSVLSQDVQSYEEMVERATFFAVQEEFADKGDLLVVVAGIPFAQAGTTNNLRVVTVPR
>Mature_472_residues
MRRHRRAKIVATVGPASNSPDMLKELFLAGVDTFRLNFSHGTQADHAKVHAAIRALEKDVQRPIGILMDLQGPKIRVGTL
RDKKIQVEAGETIRFVLNGTEGDKTSIPLPHPEIFAAVAPGHDLLIDDGRVRVRVTGLGDDWIEAKVIVSGAISNHKGVN
LPGTILELSPLTAKDRSDLTFGLDLGVDWVALSFVQKPSDVIEARGLIDGRAGIMSKIEKPAALDRIDDIIQLSDAIMVA
RGDLGVEIPNEDVPGKQKELVRACRLAVKPVIVATQMLDSMVATPTPTRAEVSDVATAIYDGADAVMLSAESASGQYPRE
AVAMMDSIIKSTEKHKMYASIIQATQPDEEQTPPHAVATAAADLASAIHAKAIVAYTSSGTSASRVARKRPSLPILAITP
NEDTSRRMCLLWGAHSVLSQDVQSYEEMVERATFFAVQEEFADKGDLLVVVAGIPFAQAGTTNNLRVVTVPR

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=493, Percent_Identity=38.5395537525355, Blast_Score=308, Evalue=5e-84,
Organism=Homo sapiens, GI33286420, Length=493, Percent_Identity=38.5395537525355, Blast_Score=308, Evalue=5e-84,
Organism=Homo sapiens, GI33286418, Length=491, Percent_Identity=38.9002036659878, Blast_Score=307, Evalue=2e-83,
Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=37.3469387755102, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=37.3469387755102, Blast_Score=288, Evalue=8e-78,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=38.1944444444444, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=38.1944444444444, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=34.453781512605, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=34.453781512605, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI310128738, Length=207, Percent_Identity=33.8164251207729, Blast_Score=112, Evalue=9e-25,
Organism=Escherichia coli, GI1787965, Length=469, Percent_Identity=39.2324093816631, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1788160, Length=482, Percent_Identity=38.5892116182573, Blast_Score=287, Evalue=9e-79,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=37.5, Blast_Score=288, Evalue=6e-78,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=38.0658436213992, Blast_Score=268, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=38.1930184804928, Blast_Score=268, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=38.1930184804928, Blast_Score=268, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=38.1930184804928, Blast_Score=268, Evalue=6e-72,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=38.1930184804928, Blast_Score=276, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6319279, Length=488, Percent_Identity=36.6803278688525, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24648964, Length=492, Percent_Identity=36.9918699186992, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI28571814, Length=492, Percent_Identity=36.9918699186992, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24648966, Length=416, Percent_Identity=39.1826923076923, Blast_Score=250, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24581235, Length=490, Percent_Identity=33.0612244897959, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24646914, Length=272, Percent_Identity=37.8676470588235, Blast_Score=158, Evalue=8e-39,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50768; Mature: 50768

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRHRRAKIVATVGPASNSPDMLKELFLAGVDTFRLNFSHGTQADHAKVHAAIRALEKDV
CCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
QRPIGILMDLQGPKIRVGTLRDKKIQVEAGETIRFVLNGTEGDKTSIPLPHPEIFAAVAP
HCCCCEEEECCCCEEEEEECCCCEEEEECCCEEEEEEECCCCCCCCCCCCCHHHEEEECC
GHDLLIDDGRVRVRVTGLGDDWIEAKVIVSGAISNHKGVNLPGTILELSPLTAKDRSDLT
CCEEEEECCEEEEEEEECCCCHHEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCE
FGLDLGVDWVALSFVQKPSDVIEARGLIDGRAGIMSKIEKPAALDRIDDIIQLSDAIMVA
EEECCCCHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCEEEEE
RGDLGVEIPNEDVPGKQKELVRACRLAVKPVIVATQMLDSMVATPTPTRAEVSDVATAIY
ECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
DGADAVMLSAESASGQYPREAVAMMDSIIKSTEKHKMYASIIQATQPDEEQTPPHAVATA
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
AADLASAIHAKAIVAYTSSGTSASRVARKRPSLPILAITPNEDTSRRMCLLWGAHSVLSQ
HHHHHHHHHHEEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECHHHHHHH
DVQSYEEMVERATFFAVQEEFADKGDLLVVVAGIPFAQAGTTNNLRVVTVPR
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MRRHRRAKIVATVGPASNSPDMLKELFLAGVDTFRLNFSHGTQADHAKVHAAIRALEKDV
CCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
QRPIGILMDLQGPKIRVGTLRDKKIQVEAGETIRFVLNGTEGDKTSIPLPHPEIFAAVAP
HCCCCEEEECCCCEEEEEECCCCEEEEECCCEEEEEEECCCCCCCCCCCCCHHHEEEECC
GHDLLIDDGRVRVRVTGLGDDWIEAKVIVSGAISNHKGVNLPGTILELSPLTAKDRSDLT
CCEEEEECCEEEEEEEECCCCHHEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCE
FGLDLGVDWVALSFVQKPSDVIEARGLIDGRAGIMSKIEKPAALDRIDDIIQLSDAIMVA
EEECCCCHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCEEEEE
RGDLGVEIPNEDVPGKQKELVRACRLAVKPVIVATQMLDSMVATPTPTRAEVSDVATAIY
ECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
DGADAVMLSAESASGQYPREAVAMMDSIIKSTEKHKMYASIIQATQPDEEQTPPHAVATA
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
AADLASAIHAKAIVAYTSSGTSASRVARKRPSLPILAITPNEDTSRRMCLLWGAHSVLSQ
HHHHHHHHHHEEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECHHHHHHH
DVQSYEEMVERATFFAVQEEFADKGDLLVVVAGIPFAQAGTTNNLRVVTVPR
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8672817 [H]