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The map label for this gene is fliM [H]

Identifier: 146341901

GI number: 146341901

Start: 5225487

End: 5226689

Strand: Reverse

Name: fliM [H]

Synonym: BRADO5035

Alternate gene names: 146341901

Gene position: 5226689-5225487 (Counterclockwise)

Preceding gene: 146341902

Following gene: 146341900

Centisome position: 70.09

GC content: 64.42

Gene sequence:

>1203_bases
ATGGCAGGCAACGATCCAGTCGACCAAGATGCGATAGCCGCGCAATGGGAAGCCTCGCTCGATTCGGAGGACCCCGCAGA
GGCCGCGGAAGCCGCAGCAGCTAACGAACTCACGGAATCGATGGCCCTGCAATGGGCCGCGATGGTCGAGGACGGCAGCC
GCGATTTCGGCGCCACCAAGAACGGCGGCGAGCGGGTGCTGTCGCAGGAGGAGATCGACAATCTCCTCGGCTTCACCGCG
GGCGAGGTCAATCTCGACGACCATTCCGGCATTCGCGCCATCATCGATTCGGCGATGGTGTCCTACGAGCGTCTGCCGAT
GCTCGAAATCGTCTTCGACCGCCTGGTGCGGCTGATGACGACCAGCTTGCGCAACTTCACCTCCGACAACGTCGAAGTCT
CGCTCGACCGCATCACCTCGGTGCGCTTCGGCGACTACATGAACTCGATCCCGCTGCCCGCCGTGCTCTCGGTGTTCAAG
GCCGAGGAGTGGGAGAATTTCGGCCTCGCGATGGTCGATTCGACGCTGATCTATTCCATCATCGACGTGCTGCTCGGCGG
TCGCCGCGGCAACACCCAGCTCCGCGTCGAGGGCCGCCCCTACACGACGATCGAGACCAATCTCGTCAAGCGCCTGATCG
AGGTGGTGCTGGCCGACGCCGAGCAGGCGTTCCGGCCGCTGTCGCCGGTGCGCTTCACCATCGACCGGCTCGAGACCAAT
CCGCGCTTCGCGGCGATCAGCCGGCCCGCCAATGCGGCCATCCTGGTGAAGCTGCGGATCGACATGGAAGACCGCGGCGG
CAACATCGAGCTGCTGCTGCCCTATGCGACCATCGAGCCGATCCGGCCGGTGCTGTTGCAGATGTTCATGGGCGAGAAGT
TCGGCCGCGATCCGGTCTGGGAAGGCCATTTCGCCACCGAGATCACCAGGGCCGAAGTCGCCGTCGACGCGGTGCTCTAC
GAGGCCGACGTGCCGCTGAAGCAATTGATGCGGCTGAAGGTGGGCGACACCCTGCCGCTCGACATTCGTCCGGATGCGCT
GGTCGCCGTCCGCTGCGGCAACACGATGCTGACGGAAGGCCGCATGGGCCGGGTCGGCGACCGCGTCGCGATCCGTGTCA
CCAAGAATTTGCGCAAGCCGCATACGACGCTTGCGATGTTCGAAGGCGCCGAAAGCCAGAACAAGTTGATGGAGGCCCAA
TGA

Upstream 100 bases:

>100_bases
GAGCTCACCAAGCGGGTCAACGTCGCGCTGGCGCCCAACCAGGTCAACGCGGTGTTGTTCAAGGAAATCGTGATTCAGTA
AGGCACGGACGCGCAAGATC

Downstream 100 bases:

>100_bases
ACCACTCCCTCGGATTGATCATCGAAAGCCTGGTGGCCGTCCTTCTGGTGCTGACCATCGGCTATTGCATGCTGCTGAAC
TCCCGGCTGAAGCGGCTGAA

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MAGNDPVDQDAIAAQWEASLDSEDPAEAAEAAAANELTESMALQWAAMVEDGSRDFGATKNGGERVLSQEEIDNLLGFTA
GEVNLDDHSGIRAIIDSAMVSYERLPMLEIVFDRLVRLMTTSLRNFTSDNVEVSLDRITSVRFGDYMNSIPLPAVLSVFK
AEEWENFGLAMVDSTLIYSIIDVLLGGRRGNTQLRVEGRPYTTIETNLVKRLIEVVLADAEQAFRPLSPVRFTIDRLETN
PRFAAISRPANAAILVKLRIDMEDRGGNIELLLPYATIEPIRPVLLQMFMGEKFGRDPVWEGHFATEITRAEVAVDAVLY
EADVPLKQLMRLKVGDTLPLDIRPDALVAVRCGNTMLTEGRMGRVGDRVAIRVTKNLRKPHTTLAMFEGAESQNKLMEAQ

Sequences:

>Translated_400_residues
MAGNDPVDQDAIAAQWEASLDSEDPAEAAEAAAANELTESMALQWAAMVEDGSRDFGATKNGGERVLSQEEIDNLLGFTA
GEVNLDDHSGIRAIIDSAMVSYERLPMLEIVFDRLVRLMTTSLRNFTSDNVEVSLDRITSVRFGDYMNSIPLPAVLSVFK
AEEWENFGLAMVDSTLIYSIIDVLLGGRRGNTQLRVEGRPYTTIETNLVKRLIEVVLADAEQAFRPLSPVRFTIDRLETN
PRFAAISRPANAAILVKLRIDMEDRGGNIELLLPYATIEPIRPVLLQMFMGEKFGRDPVWEGHFATEITRAEVAVDAVLY
EADVPLKQLMRLKVGDTLPLDIRPDALVAVRCGNTMLTEGRMGRVGDRVAIRVTKNLRKPHTTLAMFEGAESQNKLMEAQ
>Mature_399_residues
AGNDPVDQDAIAAQWEASLDSEDPAEAAEAAAANELTESMALQWAAMVEDGSRDFGATKNGGERVLSQEEIDNLLGFTAG
EVNLDDHSGIRAIIDSAMVSYERLPMLEIVFDRLVRLMTTSLRNFTSDNVEVSLDRITSVRFGDYMNSIPLPAVLSVFKA
EEWENFGLAMVDSTLIYSIIDVLLGGRRGNTQLRVEGRPYTTIETNLVKRLIEVVLADAEQAFRPLSPVRFTIDRLETNP
RFAAISRPANAAILVKLRIDMEDRGGNIELLLPYATIEPIRPVLLQMFMGEKFGRDPVWEGHFATEITRAEVAVDAVLYE
ADVPLKQLMRLKVGDTLPLDIRPDALVAVRCGNTMLTEGRMGRVGDRVAIRVTKNLRKPHTTLAMFEGAESQNKLMEAQ

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=322, Percent_Identity=23.9130434782609, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 44310; Mature: 44179

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGNDPVDQDAIAAQWEASLDSEDPAEAAEAAAANELTESMALQWAAMVEDGSRDFGATK
CCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NGGERVLSQEEIDNLLGFTAGEVNLDDHSGIRAIIDSAMVSYERLPMLEIVFDRLVRLMT
CCHHHHHCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TSLRNFTSDNVEVSLDRITSVRFGDYMNSIPLPAVLSVFKAEEWENFGLAMVDSTLIYSI
HHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
IDVLLGGRRGNTQLRVEGRPYTTIETNLVKRLIEVVLADAEQAFRPLSPVRFTIDRLETN
HHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHCCC
PRFAAISRPANAAILVKLRIDMEDRGGNIELLLPYATIEPIRPVLLQMFMGEKFGRDPVW
CCEEEECCCCCEEEEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCC
EGHFATEITRAEVAVDAVLYEADVPLKQLMRLKVGDTLPLDIRPDALVAVRCGNTMLTEG
CCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCEEECCC
RMGRVGDRVAIRVTKNLRKPHTTLAMFEGAESQNKLMEAQ
CCCCCCCEEEEEEEHHCCCCCCEEEEECCCCHHHHHCCCC
>Mature Secondary Structure 
AGNDPVDQDAIAAQWEASLDSEDPAEAAEAAAANELTESMALQWAAMVEDGSRDFGATK
CCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NGGERVLSQEEIDNLLGFTAGEVNLDDHSGIRAIIDSAMVSYERLPMLEIVFDRLVRLMT
CCHHHHHCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TSLRNFTSDNVEVSLDRITSVRFGDYMNSIPLPAVLSVFKAEEWENFGLAMVDSTLIYSI
HHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
IDVLLGGRRGNTQLRVEGRPYTTIETNLVKRLIEVVLADAEQAFRPLSPVRFTIDRLETN
HHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHCCC
PRFAAISRPANAAILVKLRIDMEDRGGNIELLLPYATIEPIRPVLLQMFMGEKFGRDPVW
CCEEEECCCCCEEEEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCC
EGHFATEITRAEVAVDAVLYEADVPLKQLMRLKVGDTLPLDIRPDALVAVRCGNTMLTEG
CCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCEEECCC
RMGRVGDRVAIRVTKNLRKPHTTLAMFEGAESQNKLMEAQ
CCCCCCCEEEEEEEHHCCCCCCEEEEECCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1315735 [H]