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The map label for this gene is recA [H]

Identifier: 146341855

GI number: 146341855

Start: 5183567

End: 5184655

Strand: Reverse

Name: recA [H]

Synonym: BRADO4985

Alternate gene names: 146341855

Gene position: 5184655-5183567 (Counterclockwise)

Preceding gene: 146341859

Following gene: 146341851

Centisome position: 69.53

GC content: 64.37

Gene sequence:

>1089_bases
ATGTCCGCCACCGCCCTGCGTATCGTCGAAGGTTCCTCCATGGATAAGAGTAAGGCGCTCTCGGCAGCGCTGTCCCAGAT
CGAGCGTCAGTTCGGCAAGGGCTCGGTGATGAAGCTCGGCAAGAACGACCGCTCGATGGACGTCGAGACAGTGTCGACGG
GCTCTCTCGGGCTGGACATCGCGCTGGGGATCGGCGGCCTGCCGAAGGGGCGCATCGTCGAGATCTACGGGCCTGAATCG
TCGGGCAAGACGACGCTGGCACTGCATACAGTGGCGGAGGCGCAGAAGAAGGGCGGCATCTGCGCGTTCATCGATGCCGA
GCATGCGCTCGATCCGGTCTATGCGCGCAAGCTGGGGGTCAACATCGATGAGCTCCTGATCTCGCAGCCGGACACCGGCG
AGCAGGCGCTGGAGATCTGCGACACGCTGGTCCGCTCCGGTGCCGTTGACGTGCTGGTCGTCGACTCGGTGGCGGCGCTG
GTCCCGAAGGCCGAGCTCGAGGGTGAGATGGGCGAGTCGCTGCCCGGCCTGCAGGCGCGATTGATGAGCCAGGCGCTGCG
CAAGCTGACGGCCTCGATCAACAAGTCCAACACGATGGTCATCTTCATCAACCAGATCCGCATGAAGATCGGCGTGATGT
ACGGCTCGCCCGAGACCACCACGGGCGGCAACGCGCTGAAGTTCTATGCTTCGGTGCGCCTCGACATCCGCCGCATCGGC
GCGATCAAGGAGCGCGACGAGGTGATCGGCAACACCACCCGCGTCAAGGTCGTCAAGAACAAGCTGGCGCCGCCGTTCAA
GCAGGTCGAGTTCGACATCATGTATGGCGAGGGCGTGTCCAAGATGGGCGAGATCCTCGATCTCGGCGTCAAGGCCGGGA
TCGTCGAGAAGTCGGGTGCCTGGTTCTCTTATGACAGCCAGCGGCTCGGGCAGGGGCGTGAGAATTCGAAATCGTTCCTG
AAGGCGAACCCCGACATGACCGCCAAGATCGAGGCGGCCATTCGCCAGAATTCCGGCCTGATCGCCGAGCAGATCCTGGC
CGGCGCCGCCGAGCGCGACGCCGACGGAGAGGAGCCGGCGGAGGAGTAG

Upstream 100 bases:

>100_bases
TTGCGAACAAAGAACAGATCAGGTACATTCACCTCAAGATTGTTGCCCGCCCTCGTTAACCATTTGTCCATCCCGCGAAT
CCCCGCCATAAGGAGCGCCA

Downstream 100 bases:

>100_bases
GGCTCGCGGGCGATCCGAAAGCCAAAGCGGAGCGGGCGCCGGTCGGTTGACTGCGGCGCCCGTTTTCGTTGGAGCGACTG
CGGGACGGATCTTTTCAGCA

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSATALRIVEGSSMDKSKALSAALSQIERQFGKGSVMKLGKNDRSMDVETVSTGSLGLDIALGIGGLPKGRIVEIYGPES
SGKTTLALHTVAEAQKKGGICAFIDAEHALDPVYARKLGVNIDELLISQPDTGEQALEICDTLVRSGAVDVLVVDSVAAL
VPKAELEGEMGESLPGLQARLMSQALRKLTASINKSNTMVIFINQIRMKIGVMYGSPETTTGGNALKFYASVRLDIRRIG
AIKERDEVIGNTTRVKVVKNKLAPPFKQVEFDIMYGEGVSKMGEILDLGVKAGIVEKSGAWFSYDSQRLGQGRENSKSFL
KANPDMTAKIEAAIRQNSGLIAEQILAGAAERDADGEEPAEE

Sequences:

>Translated_362_residues
MSATALRIVEGSSMDKSKALSAALSQIERQFGKGSVMKLGKNDRSMDVETVSTGSLGLDIALGIGGLPKGRIVEIYGPES
SGKTTLALHTVAEAQKKGGICAFIDAEHALDPVYARKLGVNIDELLISQPDTGEQALEICDTLVRSGAVDVLVVDSVAAL
VPKAELEGEMGESLPGLQARLMSQALRKLTASINKSNTMVIFINQIRMKIGVMYGSPETTTGGNALKFYASVRLDIRRIG
AIKERDEVIGNTTRVKVVKNKLAPPFKQVEFDIMYGEGVSKMGEILDLGVKAGIVEKSGAWFSYDSQRLGQGRENSKSFL
KANPDMTAKIEAAIRQNSGLIAEQILAGAAERDADGEEPAEE
>Mature_361_residues
SATALRIVEGSSMDKSKALSAALSQIERQFGKGSVMKLGKNDRSMDVETVSTGSLGLDIALGIGGLPKGRIVEIYGPESS
GKTTLALHTVAEAQKKGGICAFIDAEHALDPVYARKLGVNIDELLISQPDTGEQALEICDTLVRSGAVDVLVVDSVAALV
PKAELEGEMGESLPGLQARLMSQALRKLTASINKSNTMVIFINQIRMKIGVMYGSPETTTGGNALKFYASVRLDIRRIGA
IKERDEVIGNTTRVKVVKNKLAPPFKQVEFDIMYGEGVSKMGEILDLGVKAGIVEKSGAWFSYDSQRLGQGRENSKSFLK
ANPDMTAKIEAAIRQNSGLIAEQILAGAAERDADGEEPAEE

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family [H]

Homologues:

Organism=Escherichia coli, GI1789051, Length=322, Percent_Identity=71.1180124223602, Blast_Score=486, Evalue=1e-138,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587 [H]

Pfam domain/function: PF00154 RecA [H]

EC number: NA

Molecular weight: Translated: 38644; Mature: 38513

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00321 RECA_1 ; PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATALRIVEGSSMDKSKALSAALSQIERQFGKGSVMKLGKNDRSMDVETVSTGSLGLDI
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCCEEE
ALGIGGLPKGRIVEIYGPESSGKTTLALHTVAEAQKKGGICAFIDAEHALDPVYARKLGV
EECCCCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHCCCEEEEECCHHCCCHHHHHHHCC
NIDELLISQPDTGEQALEICDTLVRSGAVDVLVVDSVAALVPKAELEGEMGESLPGLQAR
CHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECHHHHHCCCHHHCCCCCCCCCHHHHH
LMSQALRKLTASINKSNTMVIFINQIRMKIGVMYGSPETTTGGNALKFYASVRLDIRRIG
HHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEEHHHHHHHHC
AIKERDEVIGNTTRVKVVKNKLAPPFKQVEFDIMYGEGVSKMGEILDLGVKAGIVEKSGA
CHHHHHHHHCCCEEEEEEHHHCCCCHHHEEEEEEECCCHHHHHHHHHHHHHCCEEECCCC
WFSYDSQRLGQGRENSKSFLKANPDMTAKIEAAIRQNSGLIAEQILAGAAERDADGEEPA
EEECCHHHHCCCCCCCCHHEECCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCC
EE
CC
>Mature Secondary Structure 
SATALRIVEGSSMDKSKALSAALSQIERQFGKGSVMKLGKNDRSMDVETVSTGSLGLDI
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCCEEE
ALGIGGLPKGRIVEIYGPESSGKTTLALHTVAEAQKKGGICAFIDAEHALDPVYARKLGV
EECCCCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHCCCEEEEECCHHCCCHHHHHHHCC
NIDELLISQPDTGEQALEICDTLVRSGAVDVLVVDSVAALVPKAELEGEMGESLPGLQAR
CHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECHHHHHCCCHHHCCCCCCCCCHHHHH
LMSQALRKLTASINKSNTMVIFINQIRMKIGVMYGSPETTTGGNALKFYASVRLDIRRIG
HHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEEHHHHHHHHC
AIKERDEVIGNTTRVKVVKNKLAPPFKQVEFDIMYGEGVSKMGEILDLGVKAGIVEKSGA
CHHHHHHHHCCCEEEEEEHHHCCCCHHHEEEEEEECCCHHHHHHHHHHHHHCCEEECCCC
WFSYDSQRLGQGRENSKSFLKANPDMTAKIEAAIRQNSGLIAEQILAGAAERDADGEEPA
EEECCHHHHCCCCCCCCHHEECCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCC
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]