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The map label for this gene is fdsB [H]

Identifier: 146341846

GI number: 146341846

Start: 5168841

End: 5170397

Strand: Direct

Name: fdsB [H]

Synonym: BRADO4976

Alternate gene names: 146341846

Gene position: 5168841-5170397 (Clockwise)

Preceding gene: 146341845

Following gene: 146341847

Centisome position: 69.32

GC content: 66.8

Gene sequence:

>1557_bases
ATGACGTTGCGTCTTTTTGTCCCGTGCGATGCTGGCGCGCTCGCCGTCGGCGCCGATGAGGTCGCCCACGCGCTGCACAA
GGCGGCGCTGGAGCGCAAGCTCGATGTCGAGATCGTGCGCAACGGCTCGCGTGGGTTGTACTGGCTTGAGCCACTGGTCG
AGCTGGAGACGGCGCAGGGCCGGTTCGGCTTCGGTCCCGTCACCGCCGATGACGTGCCGTCGCTGCTCGACGCCATCATC
GACGACAAGTCGCACAAGCTCTCGCTCGGGCTCACCGAGGAGATCCCGTGGCTGAAGCGGCAGACCCGACTCACCTTCGC
CCGGTGCGGCATCATCGATCCGCTGTCGCTCGAGGACTACCGCGCGCATGGCGGCTACAAGGGCCTCACGCGGGCGCTGA
CCTTGTCGCCGGATGCGGTCATCGCCGAGGTCACCACGTCGGGCCTGCGCGGCCGCGGCGGCGCGGGCTTCCCGACCGGC
ATCAAGTGGAAGACGGTGTCGCAGACCGCGGCCGACCGCAAGTACGTCATCTGCAACGCCGACGAGGGTGACAGCGGCAC
CTTCGCCGACCGCATGCTGATGGAAGGCGATCCGTTCGTGCTGATCGAAGGCATGACCATCTGCGGCATCGCGGTGGGCG
CCACCAAGGGCTACATCTACACGCGCTCGGAATATCCGCACGCGATCGCGACCATGACCAAGGCGATCGACATCGCCAAG
AAGGCCGGTCTGCTCGGCGACAAGATCTCGGGCTCGAACTACACGTTCGAGCTGGAGGTCCGCACCGGCGCCGGCGCCTA
TGTCTGCGGCGAAGAGACCGCGCTGATGGAGAGCCTCGAGGGCAAGCGCGGCATCGTCCGCGCCAAGCCGCCGCTGCCCG
CGCATCACGGCCTGTTCGGCAAGCCGACGGTCGTCAACAACCTGCTGTCGTTCGCGGCGATCCCTTTCATCCTCGATGAA
GGCGCCAAGGCCTATGCCGATTTCGGCATGGGGCGCTCGCGCGGCACGATGCCGATCCAGATCGCCGGCAATGTGAAATT
CGGCGGCCTGTTCGAGACCGCGTTCGGCATCACGCTCGGCGAACTCGTCGACGACATCGGCGGCGGCACCTTCACCGGCC
GCCCCGTTCGTGCGGTGCAGGTCGGCGGACCGCTGGGGGCCTACTTCCCCCGCGCGCTGTTCGATACGCCGTTCGACTAC
GAGGCGTTCGCCGCCAAGGAGGGCCTGATCGGTCATGGCGGCATCGTCGTGTTCGACGACACCGTCGACATGTCGAAGCA
GGCGCGCTTCGCGATGGAGTTCTGCGCCATCGAATCCTGCGGCAAGTGCACGCCCTGCCGCATCGGCTCGACGCGCGGCG
CCGAGACCATCGACAAGATCCGCCGCGGCGAGCGCGTAGCCGAGAACATCGCTCTCGTCGAGGACCTCTGCGGTACCATG
AAGCTCGGATCGCTGTGTGCACTCGGCGGCTTCACGCCCTACCCTGTGCTCTCGGCACTGAAACATTTCCGCGAGGATTT
CGGTCCTCAGCAACCGCGCCTGCAGGCTGCGGAATAG

Upstream 100 bases:

>100_bases
TCGGCCTGTGCGCGACCGCGCCGTCTGCGATGATCGACGGCCGGCTGGTCGGCCGGCTCGATGAGGCCCGGATCGATGCT
CTCGTCTCGGAGGCCCAGCG

Downstream 100 bases:

>100_bases
ACATTCGGTAATAAGGACACGGCCATGTCGCTCATCCACGAAACCGATTACGGCACCCCGCTCTCCAAGTCCGAGAAGAT
GGTGACGCTGACCATCGACG

Product: putative formate dehydrogenase subunit beta

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MTLRLFVPCDAGALAVGADEVAHALHKAALERKLDVEIVRNGSRGLYWLEPLVELETAQGRFGFGPVTADDVPSLLDAII
DDKSHKLSLGLTEEIPWLKRQTRLTFARCGIIDPLSLEDYRAHGGYKGLTRALTLSPDAVIAEVTTSGLRGRGGAGFPTG
IKWKTVSQTAADRKYVICNADEGDSGTFADRMLMEGDPFVLIEGMTICGIAVGATKGYIYTRSEYPHAIATMTKAIDIAK
KAGLLGDKISGSNYTFELEVRTGAGAYVCGEETALMESLEGKRGIVRAKPPLPAHHGLFGKPTVVNNLLSFAAIPFILDE
GAKAYADFGMGRSRGTMPIQIAGNVKFGGLFETAFGITLGELVDDIGGGTFTGRPVRAVQVGGPLGAYFPRALFDTPFDY
EAFAAKEGLIGHGGIVVFDDTVDMSKQARFAMEFCAIESCGKCTPCRIGSTRGAETIDKIRRGERVAENIALVEDLCGTM
KLGSLCALGGFTPYPVLSALKHFREDFGPQQPRLQAAE

Sequences:

>Translated_518_residues
MTLRLFVPCDAGALAVGADEVAHALHKAALERKLDVEIVRNGSRGLYWLEPLVELETAQGRFGFGPVTADDVPSLLDAII
DDKSHKLSLGLTEEIPWLKRQTRLTFARCGIIDPLSLEDYRAHGGYKGLTRALTLSPDAVIAEVTTSGLRGRGGAGFPTG
IKWKTVSQTAADRKYVICNADEGDSGTFADRMLMEGDPFVLIEGMTICGIAVGATKGYIYTRSEYPHAIATMTKAIDIAK
KAGLLGDKISGSNYTFELEVRTGAGAYVCGEETALMESLEGKRGIVRAKPPLPAHHGLFGKPTVVNNLLSFAAIPFILDE
GAKAYADFGMGRSRGTMPIQIAGNVKFGGLFETAFGITLGELVDDIGGGTFTGRPVRAVQVGGPLGAYFPRALFDTPFDY
EAFAAKEGLIGHGGIVVFDDTVDMSKQARFAMEFCAIESCGKCTPCRIGSTRGAETIDKIRRGERVAENIALVEDLCGTM
KLGSLCALGGFTPYPVLSALKHFREDFGPQQPRLQAAE
>Mature_517_residues
TLRLFVPCDAGALAVGADEVAHALHKAALERKLDVEIVRNGSRGLYWLEPLVELETAQGRFGFGPVTADDVPSLLDAIID
DKSHKLSLGLTEEIPWLKRQTRLTFARCGIIDPLSLEDYRAHGGYKGLTRALTLSPDAVIAEVTTSGLRGRGGAGFPTGI
KWKTVSQTAADRKYVICNADEGDSGTFADRMLMEGDPFVLIEGMTICGIAVGATKGYIYTRSEYPHAIATMTKAIDIAKK
AGLLGDKISGSNYTFELEVRTGAGAYVCGEETALMESLEGKRGIVRAKPPLPAHHGLFGKPTVVNNLLSFAAIPFILDEG
AKAYADFGMGRSRGTMPIQIAGNVKFGGLFETAFGITLGELVDDIGGGTFTGRPVRAVQVGGPLGAYFPRALFDTPFDYE
AFAAKEGLIGHGGIVVFDDTVDMSKQARFAMEFCAIESCGKCTPCRIGSTRGAETIDKIRRGERVAENIALVEDLCGTMK
LGSLCALGGFTPYPVLSALKHFREDFGPQQPRLQAAE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=396, Percent_Identity=37.6262626262626, Blast_Score=262, Evalue=6e-70,
Organism=Homo sapiens, GI260656005, Length=396, Percent_Identity=37.6262626262626, Blast_Score=262, Evalue=6e-70,
Organism=Escherichia coli, GI1788620, Length=405, Percent_Identity=41.4814814814815, Blast_Score=308, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI17531727, Length=399, Percent_Identity=38.8471177944862, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI19920794, Length=406, Percent_Identity=37.6847290640394, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI45550452, Length=368, Percent_Identity=37.2282608695652, Blast_Score=232, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24653819, Length=381, Percent_Identity=29.6587926509186, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24653817, Length=332, Percent_Identity=28.0120481927711, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55415; Mature: 55284

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRLFVPCDAGALAVGADEVAHALHKAALERKLDVEIVRNGSRGLYWLEPLVELETAQG
CEEEEEEECCCCEEEECHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECHHHHHHCCCC
RFGFGPVTADDVPSLLDAIIDDKSHKLSLGLTEEIPWLKRQTRLTFARCGIIDPLSLEDY
CCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
RAHGGYKGLTRALTLSPDAVIAEVTTSGLRGRGGAGFPTGIKWKTVSQTAADRKYVICNA
HHCCCCCCHHEEEEECCCCEEEEEHHCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEC
DEGDSGTFADRMLMEGDPFVLIEGMTICGIAVGATKGYIYTRSEYPHAIATMTKAIDIAK
CCCCCCCHHHHHEECCCCEEEEECCEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHH
KAGLLGDKISGSNYTFELEVRTGAGAYVCGEETALMESLEGKRGIVRAKPPLPAHHGLFG
HCCCCCCCCCCCCEEEEEEEECCCCEEEECCHHHHHHHCCCCCCEEECCCCCCCCCCCCC
KPTVVNNLLSFAAIPFILDEGAKAYADFGMGRSRGTMPIQIAGNVKFGGLFETAFGITLG
CCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEEECCCEECCHHHHHHHCCHH
ELVDDIGGGTFTGRPVRAVQVGGPLGAYFPRALFDTPFDYEAFAAKEGLIGHGGIVVFDD
HHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHCCCCEECCCEEEEEC
TVDMSKQARFAMEFCAIESCGKCTPCRIGSTRGAETIDKIRRGERVAENIALVEDLCGTM
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLGSLCALGGFTPYPVLSALKHFREDFGPQQPRLQAAE
HHCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TLRLFVPCDAGALAVGADEVAHALHKAALERKLDVEIVRNGSRGLYWLEPLVELETAQG
EEEEEEECCCCEEEECHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECHHHHHHCCCC
RFGFGPVTADDVPSLLDAIIDDKSHKLSLGLTEEIPWLKRQTRLTFARCGIIDPLSLEDY
CCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
RAHGGYKGLTRALTLSPDAVIAEVTTSGLRGRGGAGFPTGIKWKTVSQTAADRKYVICNA
HHCCCCCCHHEEEEECCCCEEEEEHHCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEC
DEGDSGTFADRMLMEGDPFVLIEGMTICGIAVGATKGYIYTRSEYPHAIATMTKAIDIAK
CCCCCCCHHHHHEECCCCEEEEECCEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHH
KAGLLGDKISGSNYTFELEVRTGAGAYVCGEETALMESLEGKRGIVRAKPPLPAHHGLFG
HCCCCCCCCCCCCEEEEEEEECCCCEEEECCHHHHHHHCCCCCCEEECCCCCCCCCCCCC
KPTVVNNLLSFAAIPFILDEGAKAYADFGMGRSRGTMPIQIAGNVKFGGLFETAFGITLG
CCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEEECCCEECCHHHHHHHCCHH
ELVDDIGGGTFTGRPVRAVQVGGPLGAYFPRALFDTPFDYEAFAAKEGLIGHGGIVVFDD
HHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHCCCCEECCCEEEEEC
TVDMSKQARFAMEFCAIESCGKCTPCRIGSTRGAETIDKIRRGERVAENIALVEDLCGTM
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLGSLCALGGFTPYPVLSALKHFREDFGPQQPRLQAAE
HHCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]