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The map label for this gene is cynR [H]

Identifier: 146341844

GI number: 146341844

Start: 5167213

End: 5168169

Strand: Reverse

Name: cynR [H]

Synonym: BRADO4974

Alternate gene names: 146341844

Gene position: 5168169-5167213 (Counterclockwise)

Preceding gene: 146341850

Following gene: 146341843

Centisome position: 69.31

GC content: 66.14

Gene sequence:

>957_bases
GTGTGGCCGAACGTTTCCGAAAACGTGCGGAACCAGGCCATTCAACCGTTGGAAGGCCGACCTTTGATCGACAAGCTCGA
ACTGCTGCTGGCGCTGGCCAAGGAACGTCATTTCGGCCGCGCGGCCGAGGCCTGCGGCGTGACCCAGCCGACCATGTCGA
CCAGCATCAAGCAGCTCGAGGAGATCCTTGGCGTCATGCTGGTGCAGCGCGGTTCCAGGTTCCAGGGCTTCACGCCGGAG
GGCGAGCGGACGCTGGACTGGGCCCGCCGGATCGTCGGCGACTTCCGCGCGATGCGCCAGGAGATCAACGGCCTGAAGGA
CAAGCTGTCGGGCGAGATCCGCCTCGCGGCGGTGCCGACCGTGCTCGGCATGGTGGCGTCGCTGACGACCCCGTTTCGCG
CCAAGCATCCGGAGGTCCGGTTCCGCATCATGTCGTGCACGTCGGCCGACGTGCTGGGCCTCTTGGAGAACCTCGAGGTC
GATGCCGGGCTGACCTATATCGAGAACGAGCCGATCGGCCGCGTCCGCACCATTCCGCTCTACAACGAAAGCTACCGTCT
TCTGACGGCGCCGGACGCGATGTTCGGCGATCGCGACCAGGTGACCTGGCAGGAGGTGGGGCAGGTACCGCTGTGCCTGC
TGACGCCGGACATGCAGAACCGCCGCATCATCGACCGCGCGCTGAAGGCCGCCGGCAACGAGGTCACGCCGACCCTAACG
TCGAACTCGCTGCTGGTGCTGTACACCCATGTGAAGACCGGCCGCTGGGCGAGCGTCATGCCCGCCAAGCTCGCTGAGAC
GCTGGGTTTGGCTGACAAGGTGCGCTCGATCCCGATCATCGATCCCGTGATCAACTACCAGATCGGCCTCGTCATTCCGC
AGCGCGACCCGATGACCCCGCTGATCGCCTCGCTGGTCCAGATCGCCCGCGAGGTCGCGCCGAGCCTGCAGGCGTAG

Upstream 100 bases:

>100_bases
TCGTGAGATAGCTACAAGTTGACGAAAAACCGACCTTCGCGCGACCGAGACGCGGCGCGTTTTACCGTGTCCGGCACACC
CTTGCCGTGATAGGGTTTCC

Downstream 100 bases:

>100_bases
GCAGCGATTCTCGTCAGCGAATGTTAGCTCCGCTTGACGGCCTCCGAAGTGTAATTGTGTGCAAGCGGCCCTGGATTGCT
TCGCTGCGCTCGCAATGACG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MWPNVSENVRNQAIQPLEGRPLIDKLELLLALAKERHFGRAAEACGVTQPTMSTSIKQLEEILGVMLVQRGSRFQGFTPE
GERTLDWARRIVGDFRAMRQEINGLKDKLSGEIRLAAVPTVLGMVASLTTPFRAKHPEVRFRIMSCTSADVLGLLENLEV
DAGLTYIENEPIGRVRTIPLYNESYRLLTAPDAMFGDRDQVTWQEVGQVPLCLLTPDMQNRRIIDRALKAAGNEVTPTLT
SNSLLVLYTHVKTGRWASVMPAKLAETLGLADKVRSIPIIDPVINYQIGLVIPQRDPMTPLIASLVQIAREVAPSLQA

Sequences:

>Translated_318_residues
MWPNVSENVRNQAIQPLEGRPLIDKLELLLALAKERHFGRAAEACGVTQPTMSTSIKQLEEILGVMLVQRGSRFQGFTPE
GERTLDWARRIVGDFRAMRQEINGLKDKLSGEIRLAAVPTVLGMVASLTTPFRAKHPEVRFRIMSCTSADVLGLLENLEV
DAGLTYIENEPIGRVRTIPLYNESYRLLTAPDAMFGDRDQVTWQEVGQVPLCLLTPDMQNRRIIDRALKAAGNEVTPTLT
SNSLLVLYTHVKTGRWASVMPAKLAETLGLADKVRSIPIIDPVINYQIGLVIPQRDPMTPLIASLVQIAREVAPSLQA
>Mature_318_residues
MWPNVSENVRNQAIQPLEGRPLIDKLELLLALAKERHFGRAAEACGVTQPTMSTSIKQLEEILGVMLVQRGSRFQGFTPE
GERTLDWARRIVGDFRAMRQEINGLKDKLSGEIRLAAVPTVLGMVASLTTPFRAKHPEVRFRIMSCTSADVLGLLENLEV
DAGLTYIENEPIGRVRTIPLYNESYRLLTAPDAMFGDRDQVTWQEVGQVPLCLLTPDMQNRRIIDRALKAAGNEVTPTLT
SNSLLVLYTHVKTGRWASVMPAKLAETLGLADKVRSIPIIDPVINYQIGLVIPQRDPMTPLIASLVQIAREVAPSLQA

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790399, Length=292, Percent_Identity=27.7397260273973, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI157672245, Length=264, Percent_Identity=26.5151515151515, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788748, Length=297, Percent_Identity=24.5791245791246, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI145693105, Length=279, Percent_Identity=24.0143369175627, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786401, Length=173, Percent_Identity=30.635838150289, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI2367136, Length=168, Percent_Identity=30.3571428571429, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1790208, Length=155, Percent_Identity=30.3225806451613, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87081904, Length=171, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787879, Length=283, Percent_Identity=23.6749116607774, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788296, Length=228, Percent_Identity=25, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35282; Mature: 35282

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWPNVSENVRNQAIQPLEGRPLIDKLELLLALAKERHFGRAAEACGVTQPTMSTSIKQLE
CCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHH
EILGVMLVQRGSRFQGFTPEGERTLDWARRIVGDFRAMRQEINGLKDKLSGEIRLAAVPT
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHH
VLGMVASLTTPFRAKHPEVRFRIMSCTSADVLGLLENLEVDAGLTYIENEPIGRVRTIPL
HHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEE
YNESYRLLTAPDAMFGDRDQVTWQEVGQVPLCLLTPDMQNRRIIDRALKAAGNEVTPTLT
ECCCEEEEECCCHHCCCCCCCCHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEC
SNSLLVLYTHVKTGRWASVMPAKLAETLGLADKVRSIPIIDPVINYQIGLVIPQRDPMTP
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEEEECCCCCCHH
LIASLVQIAREVAPSLQA
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MWPNVSENVRNQAIQPLEGRPLIDKLELLLALAKERHFGRAAEACGVTQPTMSTSIKQLE
CCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHH
EILGVMLVQRGSRFQGFTPEGERTLDWARRIVGDFRAMRQEINGLKDKLSGEIRLAAVPT
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHH
VLGMVASLTTPFRAKHPEVRFRIMSCTSADVLGLLENLEVDAGLTYIENEPIGRVRTIPL
HHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEE
YNESYRLLTAPDAMFGDRDQVTWQEVGQVPLCLLTPDMQNRRIIDRALKAAGNEVTPTLT
ECCCEEEEECCCHHCCCCCCCCHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEC
SNSLLVLYTHVKTGRWASVMPAKLAETLGLADKVRSIPIIDPVINYQIGLVIPQRDPMTP
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEEEECCCCCCHH
LIASLVQIAREVAPSLQA
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]