Click here to switch to the map view.

The map label for this gene is fieF [H]

Identifier: 146341838

GI number: 146341838

Start: 5158848

End: 5160236

Strand: Direct

Name: fieF [H]

Synonym: BRADO4967

Alternate gene names: 146341838

Gene position: 5158848-5160236 (Clockwise)

Preceding gene: 146341837

Following gene: 146341839

Centisome position: 69.19

GC content: 69.33

Gene sequence:

>1389_bases
ATGAGCGACGCCGCCTCCCCCATCGAGTCCGGGAAAGCCCGTGTCGCCGCGATCTCGATCGCGGCGAGCGCCAGCATGGC
GGCGGCGAAGTTCATCGTCGGTGTTCTGATCGGCAGCCTCGCTTTGGTCTCGGAAGCGCTGCACAGCGCAGTCGACGTCG
TCGCCACCATCATCACCTGGATGGTGGTTCGGGTATCGGACAGGCCCGCGGACGACGAGCATCACTACGGCCATGGCAAG
CTGGAGAGCCTGTCCGCGCTCGGCGTCATCGCGCTGCTCTACGTGCTCGCCGGCGGCGTGCTGGTGGAGTCGGTGAGCCG
GCTGCGCGAAGCGGCGCCGCCGCCATCGCTGTCGGTCATTCCGTTCGTGGTGCTGCTGATCGACATCGCCGTCAACTTCT
GGCGCGCCCGCGCGCTGCATCGCGCCGCGCACGAGACCCACAGCCAGGCGCTCGCCGCCGACGCGCTGCATTTCGCGTCC
GACGTGCTCGGCTCGATCGCGGTCATCGCCGGCCTCGTGCTGACCGCGCTTGGCTTCTGGTGGGGGGACTCCGCCGCCGC
CATCGCGGTGGCCGCGATGATCTCGGTGCTCGGCCTCCGGCTCGCGCGCTCGACCGTCGAGACCCTGCTCGACCGCGCGC
CTGATGGCGCCGCCGAGAAAGCCGACGCCGCGATCCGTTCGGTCGCCGGCGTCGTCGACGTCGAGCGGCTGCGCGTGCGC
CTCGTCGGCGCCACGCATTTCGTCGACGCCACCGTGCAGGTGCCCCGCACCTATCCGATCGACCGCGTCGACGAGATCAA
GCGCCAGGCCCAGGCCGCCGTCGGCAAGAGCCTGTCGGAGGCCGACGTCACCTTCACCACGGTGCCGGTCGCGCTCAGCA
ATGAGAGCGTGCGCGAGCGCATCATGGTGATCGCGCGGAATTCCGGCCTCGCGATCCACCACGTCACCGTCCACGATATC
GGCGGCCGGTTGATCGTGGCTATCGACCTCGAGGTCGATGGCGACATGAGCCTCGTGGCGGCGCATGCGATCGCCCACGA
TCTGGAGCGCTCGATCCGCGAGGAGTTCGGCGCCGATGTCGAGGTCGACACCCATATAGAGCCGCTCGAGCCCGAGCTGC
CGTTCGGCATCGACGCGCCGGACGAGCGCGTCCGGCACATCGCCGACGCGCTGCAGCGCCTCGCCGCCGAGACGGACATG
TACGACGTCCACAATGTCCGCGTCCGCAACACCGAGGCCGGCGAGATCGTCAACTTCCATTGCCGCGCCGCGCCGTCGAT
GAGCGTGCTGAAGGTGCACGACAATGTCGACCACATCGAGCGCGGGTTACGTCGCGCCTTCCCGACCGTGAAGCGCGTCA
TCAGTCACGCCGAGCCCCCGCACGGCTGA

Upstream 100 bases:

>100_bases
AACGGCGCGCGATCCTGCAGCAGGTCATGCAGGCTGTTATGCATCTGGGCCGCGCGATCCCGGCTTTCACGGCGCGGCGT
CGGCTGCTACATCCGCGGCT

Downstream 100 bases:

>100_bases
AGCCCAGACGTCACGCTGCGTTCTCGCTTCGGACTCATCTCGCCGGCTTTTCGCCATGGCAAATCCGCATTGGATAAGAT
TTATTAGCTTTTGCCAAATC

Product: putative cation-efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSDAASPIESGKARVAAISIAASASMAAAKFIVGVLIGSLALVSEALHSAVDVVATIITWMVVRVSDRPADDEHHYGHGK
LESLSALGVIALLYVLAGGVLVESVSRLREAAPPPSLSVIPFVVLLIDIAVNFWRARALHRAAHETHSQALAADALHFAS
DVLGSIAVIAGLVLTALGFWWGDSAAAIAVAAMISVLGLRLARSTVETLLDRAPDGAAEKADAAIRSVAGVVDVERLRVR
LVGATHFVDATVQVPRTYPIDRVDEIKRQAQAAVGKSLSEADVTFTTVPVALSNESVRERIMVIARNSGLAIHHVTVHDI
GGRLIVAIDLEVDGDMSLVAAHAIAHDLERSIREEFGADVEVDTHIEPLEPELPFGIDAPDERVRHIADALQRLAAETDM
YDVHNVRVRNTEAGEIVNFHCRAAPSMSVLKVHDNVDHIERGLRRAFPTVKRVISHAEPPHG

Sequences:

>Translated_462_residues
MSDAASPIESGKARVAAISIAASASMAAAKFIVGVLIGSLALVSEALHSAVDVVATIITWMVVRVSDRPADDEHHYGHGK
LESLSALGVIALLYVLAGGVLVESVSRLREAAPPPSLSVIPFVVLLIDIAVNFWRARALHRAAHETHSQALAADALHFAS
DVLGSIAVIAGLVLTALGFWWGDSAAAIAVAAMISVLGLRLARSTVETLLDRAPDGAAEKADAAIRSVAGVVDVERLRVR
LVGATHFVDATVQVPRTYPIDRVDEIKRQAQAAVGKSLSEADVTFTTVPVALSNESVRERIMVIARNSGLAIHHVTVHDI
GGRLIVAIDLEVDGDMSLVAAHAIAHDLERSIREEFGADVEVDTHIEPLEPELPFGIDAPDERVRHIADALQRLAAETDM
YDVHNVRVRNTEAGEIVNFHCRAAPSMSVLKVHDNVDHIERGLRRAFPTVKRVISHAEPPHG
>Mature_461_residues
SDAASPIESGKARVAAISIAASASMAAAKFIVGVLIGSLALVSEALHSAVDVVATIITWMVVRVSDRPADDEHHYGHGKL
ESLSALGVIALLYVLAGGVLVESVSRLREAAPPPSLSVIPFVVLLIDIAVNFWRARALHRAAHETHSQALAADALHFASD
VLGSIAVIAGLVLTALGFWWGDSAAAIAVAAMISVLGLRLARSTVETLLDRAPDGAAEKADAAIRSVAGVVDVERLRVRL
VGATHFVDATVQVPRTYPIDRVDEIKRQAQAAVGKSLSEADVTFTTVPVALSNESVRERIMVIARNSGLAIHHVTVHDIG
GRLIVAIDLEVDGDMSLVAAHAIAHDLERSIREEFGADVEVDTHIEPLEPELPFGIDAPDERVRHIADALQRLAAETDMY
DVHNVRVRNTEAGEIVNFHCRAAPSMSVLKVHDNVDHIERGLRRAFPTVKRVISHAEPPHG

Specific function: Cation-efflux transporter that may have a role in detoxification [H]

COG id: COG0053

COG function: function code P; Predicted Co/Zn/Cd cation transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. FieF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790349, Length=259, Percent_Identity=30.8880308880309, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002524 [H]

Pfam domain/function: PF01545 Cation_efflux [H]

EC number: NA

Molecular weight: Translated: 49299; Mature: 49168

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAASPIESGKARVAAISIAASASMAAAKFIVGVLIGSLALVSEALHSAVDVVATIITW
CCCCCCCHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVVRVSDRPADDEHHYGHGKLESLSALGVIALLYVLAGGVLVESVSRLREAAPPPSLSVI
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
PFVVLLIDIAVNFWRARALHRAAHETHSQALAADALHFASDVLGSIAVIAGLVLTALGFW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGDSAAAIAVAAMISVLGLRLARSTVETLLDRAPDGAAEKADAAIRSVAGVVDVERLRVR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LVGATHFVDATVQVPRTYPIDRVDEIKRQAQAAVGKSLSEADVTFTTVPVALSNESVRER
HCCHHHHEECEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCHHHHHH
IMVIARNSGLAIHHVTVHDIGGRLIVAIDLEVDGDMSLVAAHAIAHDLERSIREEFGADV
EEEEEECCCEEEEEEEEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
EVDTHIEPLEPELPFGIDAPDERVRHIADALQRLAAETDMYDVHNVRVRNTEAGEIVNFH
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCEEEEE
CRAAPSMSVLKVHDNVDHIERGLRRAFPTVKRVISHAEPPHG
ECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDAASPIESGKARVAAISIAASASMAAAKFIVGVLIGSLALVSEALHSAVDVVATIITW
CCCCCCHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVVRVSDRPADDEHHYGHGKLESLSALGVIALLYVLAGGVLVESVSRLREAAPPPSLSVI
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
PFVVLLIDIAVNFWRARALHRAAHETHSQALAADALHFASDVLGSIAVIAGLVLTALGFW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGDSAAAIAVAAMISVLGLRLARSTVETLLDRAPDGAAEKADAAIRSVAGVVDVERLRVR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LVGATHFVDATVQVPRTYPIDRVDEIKRQAQAAVGKSLSEADVTFTTVPVALSNESVRER
HCCHHHHEECEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCHHHHHH
IMVIARNSGLAIHHVTVHDIGGRLIVAIDLEVDGDMSLVAAHAIAHDLERSIREEFGADV
EEEEEECCCEEEEEEEEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
EVDTHIEPLEPELPFGIDAPDERVRHIADALQRLAAETDMYDVHNVRVRNTEAGEIVNFH
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCEEEEE
CRAAPSMSVLKVHDNVDHIERGLRRAFPTVKRVISHAEPPHG
ECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA