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The map label for this gene is rfbF [H]

Identifier: 146341730

GI number: 146341730

Start: 5040488

End: 5041285

Strand: Reverse

Name: rfbF [H]

Synonym: BRADO4835

Alternate gene names: 146341730

Gene position: 5041285-5040488 (Counterclockwise)

Preceding gene: 146341732

Following gene: 146341729

Centisome position: 67.61

GC content: 61.78

Gene sequence:

>798_bases
ATGAAGGTCGTCTTGTTCTGTGGGGGACTGGGGACGCGGATCCGGGAATATTCCGAGAGCGTTCCGAAGCCGATGATTCC
GATCGGCCACCAGCCGATCCTTTGGCACGTGATGCAGTATTACAGCCAGTACGGCCACCGCGACTTCGTGCTCTGCCTCG
GCTACAAGGCCAACGTCGTCAAGGACTACTTCCTGAATTACAATCAGGCCGCTACGACCGACTGCACGATCTCGCAATTC
GGCAAGAAGATCGAGCTCCTGGGCGAGCGCCCGGCCGACTGGCGGGTGTCGCTGGTCGACACTGGGATCTGGCGCAACAT
CGGTCAGCGCCTGAGGGCCGTCCGGCATCTCGTCGAGGGCGAGGAGATCTTTCTCGCCAATTACAGCGACGGCCTGACCG
ATGCGCCGCTGCCCGACATGATCGACCGCTTCAAGCGCAGCGGCAAGATCGGCTGCTTCATCGCCATCCATCCGCCGATC
AGCTTCCATCTGGCCGAGTTCGACGACGACGGCACGGTCGGCCGCATGGCCGCGAGCAATGATTCCAACATCTGGATCAA
CGGCGGCTACTTCATCTTCCGCAAGGAGATCTTCGATTACATCGAGGAGGGCGACGAGCTCGTGGTGCAGCCGTTCAAGC
GGCTGATCGATCGTGGCCTGCTGATGGCCTATCGCTACGAAGGCTTCTGGCGGGCCATGGATACGCTCCGGGACCGCCAG
GTGCTCGAGGAGATGATCGAGCGCGGCGATACGCCCTGGCATCTCGGCGCGAACCTTCCCGCCGTGGTGAACGGATGA

Upstream 100 bases:

>100_bases
CTTGCCGCCAAGGGCGCGGCCAGTCTTCCGGGCGGCCGTGGGGTTGGACGAAAATGATTGCTGATTTCTGAGTTTTAATG
AAGCCGAAGGACGTCGAGCC

Downstream 100 bases:

>100_bases
GGGGATTGCAGCTCGCCGGTCGCGGCGACCGTTTGTCGGTGCTTTGTCTCGGCGCGCATTCCGACGACATCGAGATCGGC
GCGGGCGGCACGCTTCTGAC

Product: putative sugar-phosphate nucleotidyltransferase glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKVVLFCGGLGTRIREYSESVPKPMIPIGHQPILWHVMQYYSQYGHRDFVLCLGYKANVVKDYFLNYNQAATTDCTISQF
GKKIELLGERPADWRVSLVDTGIWRNIGQRLRAVRHLVEGEEIFLANYSDGLTDAPLPDMIDRFKRSGKIGCFIAIHPPI
SFHLAEFDDDGTVGRMAASNDSNIWINGGYFIFRKEIFDYIEEGDELVVQPFKRLIDRGLLMAYRYEGFWRAMDTLRDRQ
VLEEMIERGDTPWHLGANLPAVVNG

Sequences:

>Translated_265_residues
MKVVLFCGGLGTRIREYSESVPKPMIPIGHQPILWHVMQYYSQYGHRDFVLCLGYKANVVKDYFLNYNQAATTDCTISQF
GKKIELLGERPADWRVSLVDTGIWRNIGQRLRAVRHLVEGEEIFLANYSDGLTDAPLPDMIDRFKRSGKIGCFIAIHPPI
SFHLAEFDDDGTVGRMAASNDSNIWINGGYFIFRKEIFDYIEEGDELVVQPFKRLIDRGLLMAYRYEGFWRAMDTLRDRQ
VLEEMIERGDTPWHLGANLPAVVNG
>Mature_265_residues
MKVVLFCGGLGTRIREYSESVPKPMIPIGHQPILWHVMQYYSQYGHRDFVLCLGYKANVVKDYFLNYNQAATTDCTISQF
GKKIELLGERPADWRVSLVDTGIWRNIGQRLRAVRHLVEGEEIFLANYSDGLTDAPLPDMIDRFKRSGKIGCFIAIHPPI
SFHLAEFDDDGTVGRMAASNDSNIWINGGYFIFRKEIFDYIEEGDELVVQPFKRLIDRGLLMAYRYEGFWRAMDTLRDRQ
VLEEMIERGDTPWHLGANLPAVVNG

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320148, Length=245, Percent_Identity=23.265306122449, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 30446; Mature: 30446

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVLFCGGLGTRIREYSESVPKPMIPIGHQPILWHVMQYYSQYGHRDFVLCLGYKANVV
CEEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHH
KDYFLNYNQAATTDCTISQFGKKIELLGERPADWRVSLVDTGIWRNIGQRLRAVRHLVEG
HHHHHCCCCCCCCCCCHHHHCCCEEHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCC
EEIFLANYSDGLTDAPLPDMIDRFKRSGKIGCFIAIHPPISFHLAEFDDDGTVGRMAASN
CEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCEEEEEECC
DSNIWINGGYFIFRKEIFDYIEEGDELVVQPFKRLIDRGLLMAYRYEGFWRAMDTLRDRQ
CCEEEEECCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLEEMIERGDTPWHLGANLPAVVNG
HHHHHHHCCCCCEEECCCCCEEECC
>Mature Secondary Structure
MKVVLFCGGLGTRIREYSESVPKPMIPIGHQPILWHVMQYYSQYGHRDFVLCLGYKANVV
CEEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHH
KDYFLNYNQAATTDCTISQFGKKIELLGERPADWRVSLVDTGIWRNIGQRLRAVRHLVEG
HHHHHCCCCCCCCCCCHHHHCCCEEHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCC
EEIFLANYSDGLTDAPLPDMIDRFKRSGKIGCFIAIHPPISFHLAEFDDDGTVGRMAASN
CEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCEEEEEECC
DSNIWINGGYFIFRKEIFDYIEEGDELVVQPFKRLIDRGLLMAYRYEGFWRAMDTLRDRQ
CCEEEEECCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLEEMIERGDTPWHLGANLPAVVNG
HHHHHHHCCCCCEEECCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]