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The map label for this gene is pyrB [H]

Identifier: 146341412

GI number: 146341412

Start: 4701291

End: 4702244

Strand: Direct

Name: pyrB [H]

Synonym: BRADO4502

Alternate gene names: 146341412

Gene position: 4701291-4702244 (Clockwise)

Preceding gene: 146341408

Following gene: 146341413

Centisome position: 63.05

GC content: 65.51

Gene sequence:

>954_bases
ATGGCAACCTCTCCGAAATCGACGTTTGTCCTCGGGCACCGGCATTTGCTGGGAATCGAGGGCCTTTCCACCGCCGACAT
CACCGGTCTCCTCGATCTGTCCGAGGAGTATGTCGAACTGAACCGTCAGGTCGACAAGAAGCGTACCGTGCTGCGCGGCC
GGACCCAGGTCAACCTGTTCTTCGAGGCCTCGACGCGGACGCAATCCTCGTTCGAGCTGGCCGGCAAGCGGCTTGGCGCC
GACGTCATGAACATGTCGGTGTCGTCCTCGTCGATCAAGAAGGGCGAGACCCTGATCGACACCGCGGTCACGCTCAACGC
CATGCATCCGGACATCCTGGTGGTCCGTCACCACGCCTCCGGCGCGGTCGAGCTCTTGGCCCGCAAGGTCGACGGCTCCG
TGATCAATGCCGGCGACGGCGCGCATGAGCATCCGACGCAGGCGCTGCTGGATGCGCTCACCATCCGCCGCAACAAGGGC
CGCATCGAGGGCCTGGTCATCGCGATCTGCGGCGACGTGCTGCATTCGCGCGTCGCGCGCTCCAACATCCTCCTGCTGAA
CACAATGGGCGCCCGCGTCCGCGTCGTCGCGCCCTCCACTTTGCTGCCGCCGGGCATCGAGCGGATGGGCGTCGAGGTCG
CGCGCGACATGCGCGAAGGCTTGAACGGCGCCGACATCGTCATGATGCTCCGCCTGCAGCGCGAGCGCATGAACGGCTCC
TTCGTGCCGTCGTCGTCGGAATATTTCCAGTATTTCGGCCTCGACCAGAAGAAGCTCGCTTACGCCAAGCCGGACGCGCT
CGTCATGCATCCGGGACCGATGAACCGCGGCGTCGAGATCGACTCCATCGTCGCCGACGGCGCGCAGTCGCTGATCCGGG
AACAGGTCGAAATGGGCGTCGCCGTGCGCATGGCCGTGCTCGAGGCGCTGGCCCGCAACCTGCCCAACGCTTGA

Upstream 100 bases:

>100_bases
GGGCCGTGCCCACGCCTGGACAGACGGAACGGGGGATAACTCGGAACCCCGGCTTTCGGCCTTGCCCGCCATGGAAACCG
CGCCTATAGCTCTGGCTTTA

Downstream 100 bases:

>100_bases
GATCAAAGTCGGGACGAAACGACATGCTGACCGATCGCCGCCCGATCCTGCTCGCCAATGCGCGCGTCATCGATCCCTCC
CGCGACATCGACGGTCCCGG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MATSPKSTFVLGHRHLLGIEGLSTADITGLLDLSEEYVELNRQVDKKRTVLRGRTQVNLFFEASTRTQSSFELAGKRLGA
DVMNMSVSSSSIKKGETLIDTAVTLNAMHPDILVVRHHASGAVELLARKVDGSVINAGDGAHEHPTQALLDALTIRRNKG
RIEGLVIAICGDVLHSRVARSNILLLNTMGARVRVVAPSTLLPPGIERMGVEVARDMREGLNGADIVMMLRLQRERMNGS
FVPSSSEYFQYFGLDQKKLAYAKPDALVMHPGPMNRGVEIDSIVADGAQSLIREQVEMGVAVRMAVLEALARNLPNA

Sequences:

>Translated_317_residues
MATSPKSTFVLGHRHLLGIEGLSTADITGLLDLSEEYVELNRQVDKKRTVLRGRTQVNLFFEASTRTQSSFELAGKRLGA
DVMNMSVSSSSIKKGETLIDTAVTLNAMHPDILVVRHHASGAVELLARKVDGSVINAGDGAHEHPTQALLDALTIRRNKG
RIEGLVIAICGDVLHSRVARSNILLLNTMGARVRVVAPSTLLPPGIERMGVEVARDMREGLNGADIVMMLRLQRERMNGS
FVPSSSEYFQYFGLDQKKLAYAKPDALVMHPGPMNRGVEIDSIVADGAQSLIREQVEMGVAVRMAVLEALARNLPNA
>Mature_316_residues
ATSPKSTFVLGHRHLLGIEGLSTADITGLLDLSEEYVELNRQVDKKRTVLRGRTQVNLFFEASTRTQSSFELAGKRLGAD
VMNMSVSSSSIKKGETLIDTAVTLNAMHPDILVVRHHASGAVELLARKVDGSVINAGDGAHEHPTQALLDALTIRRNKGR
IEGLVIAICGDVLHSRVARSNILLLNTMGARVRVVAPSTLLPPGIERMGVEVARDMREGLNGADIVMMLRLQRERMNGSF
VPSSSEYFQYFGLDQKKLAYAKPDALVMHPGPMNRGVEIDSIVADGAQSLIREQVEMGVAVRMAVLEALARNLPNA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=319, Percent_Identity=36.9905956112853, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=33.0128205128205, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1790703, Length=235, Percent_Identity=28.936170212766, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1786469, Length=237, Percent_Identity=29.1139240506329, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=326, Percent_Identity=34.9693251533742, Blast_Score=137, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6322331, Length=307, Percent_Identity=35.1791530944625, Blast_Score=153, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24642586, Length=305, Percent_Identity=34.0983606557377, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34452; Mature: 34321

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSPKSTFVLGHRHLLGIEGLSTADITGLLDLSEEYVELNRQVDKKRTVLRGRTQVNLF
CCCCCCCEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
FEASTRTQSSFELAGKRLGADVMNMSVSSSSIKKGETLIDTAVTLNAMHPDILVVRHHAS
EEECCCCCHHHHHHHHHHCHHHHHCCCCCCHHHCCCHHHHHHHEEECCCCCEEEEEECCC
GAVELLARKVDGSVINAGDGAHEHPTQALLDALTIRRNKGRIEGLVIAICGDVLHSRVAR
CHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHH
SNILLLNTMGARVRVVAPSTLLPPGIERMGVEVARDMREGLNGADIVMMLRLQRERMNGS
CCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
FVPSSSEYFQYFGLDQKKLAYAKPDALVMHPGPMNRGVEIDSIVADGAQSLIREQVEMGV
CCCCCHHHHHHHCCCHHHHEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVRMAVLEALARNLPNA
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATSPKSTFVLGHRHLLGIEGLSTADITGLLDLSEEYVELNRQVDKKRTVLRGRTQVNLF
CCCCCCEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
FEASTRTQSSFELAGKRLGADVMNMSVSSSSIKKGETLIDTAVTLNAMHPDILVVRHHAS
EEECCCCCHHHHHHHHHHCHHHHHCCCCCCHHHCCCHHHHHHHEEECCCCCEEEEEECCC
GAVELLARKVDGSVINAGDGAHEHPTQALLDALTIRRNKGRIEGLVIAICGDVLHSRVAR
CHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHH
SNILLLNTMGARVRVVAPSTLLPPGIERMGVEVARDMREGLNGADIVMMLRLQRERMNGS
CCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
FVPSSSEYFQYFGLDQKKLAYAKPDALVMHPGPMNRGVEIDSIVADGAQSLIREQVEMGV
CCCCCHHHHHHHCCCHHHHEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVRMAVLEALARNLPNA
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA