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The map label for this gene is slt [H]

Identifier: 146341389

GI number: 146341389

Start: 4674688

End: 4677033

Strand: Direct

Name: slt [H]

Synonym: BRADO4476

Alternate gene names: 146341389

Gene position: 4674688-4677033 (Clockwise)

Preceding gene: 146341384

Following gene: 146341397

Centisome position: 62.69

GC content: 67.77

Gene sequence:

>2346_bases
GTGTCTGCCCGCGCCGCCGTATTGCGATCGACCGCCCTGTCGATGTCTCTCTCGGCGTCACTGGCCGCCGGCCTGACGCT
GGGTCTCGCCCTTGGCGGAGATATCGCGCTGGCCAAGGACAAAGTTCCTCTCCCGAAGGCGCGCCCGATCGCGCGTTCCA
TGATTCCCGCAACGCCCGCAAGAGATACCGCCGAGTCCAGAAGCAAGGTTGACGCGCGGGCGGAAGCCAAGGCCGATGTG
AAGGCGGATCTCAGGGACACCAAGAGCATCGGCAAAGGCGCTGCCGAGCCAAGCCGCGGCGCCCCCAGGGATACTACGCC
AGGGGCGGTGCCAGGTTCGGCGCCGAGTGCAATGCCCAGCGCGCTGCCGCCGCGCCAGCACGCGGCCCTCCCCGCTCCCC
GCAAGCCCATCGCTACGGCCGCGATCGCCGCGACGGCCTCGACCTCGCAGTCGGACAAGGATGCGCTCGAGAACGTCATC
GAGCTGGTGCGCAAGCGCAAGCCGGATGACGCCACCCAGGTCGAGCAGGCCATCTCGGACCCCGTGGCCCGCAAGCTCGC
GGAATGGGTCATCCTGCGCAGCGACAACAACAACGCCACCGTCGAGCGCTACCGCGCCTTCATCACCGCCAATCCGAGCT
GGCCGTCGCAGAGCTTCCTGCGCCGCCGTCTCGAGGCCGCGCTGTGGGACGACAAGCGCGACGATTCGGTGGTGTGGTCG
TGGTTCGAGAACGAATCGCCGCTGTCGGCCAAGGGCAAGTTCGCGCTCGCCCGCGTCATGCTCAACCGCGGCGACCGCGC
CAATGCCGAGCGCCTGGTTCGCGAGGCCTGGCGCAACGATGCGATGTCGGAGGAGACCGAGAACAACATCCTCGACCAGT
TCGGCGCCCTGATCTCCGGCGGCGACCACAAGGCGCGGATGGACACGCTGCTGTATGGCAGCGAGCACGAGGCAGCGCTG
CGCGCGGCCAAGCGGCTCGGTTCCGGGCACATGGCGCTGGCCAAGGCGCGCATCGCGGCCTACCGTAAGGCCGCCAACAG
CCGCGCGCTACTCGACGCGGTGCCGCATGAGCTGCATGGCGACGCCGGCTACATCTTCAGCAAGATCCAGTTGCTCAGGC
GCGACGAGAATTTCACGGAAGCGGCGCAGCTGATGCTGAGCGCGCCGAAGGATTCCGGCCGCCTGCACAATCTCGACGAA
TGGTGGATCGAGCGCCGCCTTCTGGCGCGCAAGATGATCGATACCGGCGACTACCGCAGCGCCTATCTGATCGCTCGCGA
CGCTGCGCTGCCGAATCGCGACATCTACAAGACCGAGCAGGAATTCACCGCCGGCTGGATCGCGCTGCGCTTCCTCAAAG
ATCCGGCGACGGCCGCCCAGCACTTTGCCCGCATCGGCGTCGGCAGCGTCAACCCGACCACGCTGGCGCGCGCCGGCTAT
TGGCAGGGCCGCGCGGCAGAGGCGATGGGCCGGATGCAGGAGGCGCGCGCCGCCTACACGTCAGCGGCCGAGCAGTCGAC
GAGCTATTACGGCCAGCTGGCGCGGGCCAAGCTCGGGCTGCCGCAGCTCGGCCTCAACGCTGCGCCCGGCGGCCGTGGCC
GCGGTGTGGAGCGTCTCGAAGTGGTTCGCGCGGTGCAGCTGCTGTACGACCTCGACGAGGGCGAGGTCGCGATCCCGATC
TTCGCGGATATGGGTGACAATGGCGATCCAGACGTGCTGCTCGGGCTCGCCGAGGCCGCTGGCCGGCAGAGCGATGCGCG
GGCGATGCTGCTGGTCGGCAAGGCCGCGCTCAATCGCGGGCTGCCGTTCGATCATTACGCCTATCCCGTCAACGGCATCC
CGGCGTTCCGGTCGATCGGACCCGAGGTCGAGCAGAGCGTGATTTTCTCGATCGCCCGCCAGGAAAGCGCGTTCAACCAG
GCCGTGGTGTCTCCGGCGCAGGCCTACGGGCTGATGCAGGTCACGCCCGATGCGGGCCGCTATGTCTGCAAGCGCGCCGG
CGTCAGCTTCGATCTCAACCGGATGAAGACCGATCCTGTGTATAACGCCCAGCTCGGCGCGGCCGAGCTCGGCGGCCTGC
TCGAGGACTATCGCGGCTCCTACATCATGACGTTCGCGGCCTACAATGCCGGCCGGGGCAGCGTGAAGAAATGGATCGAG
CGATATGGCGATCCGCGCGATCCCAATGTCGATGCGGTCGACTGGGTGGAGCTGATCCCGTTCGCGGAAACCCGCAACTA
CGTGCAGCGCGTGATGGAAAATCTGCAGGTCTATCGCGCCCGCTTCGGCGGCGGGTCACGGCTGCAGATCGAGGCCGATC
TGCGCCGTGGGTCCGTCGTCGAGTAG

Upstream 100 bases:

>100_bases
GGTTGCAAACGATCCCGGTACATTTAGTATTTGTTCACCTTTTCGATAGACCAAGAGCGTCAGGGATCGAAGTCATCCGC
CGCAAAGGAACGCCGTACCC

Downstream 100 bases:

>100_bases
CGAGTCGCGACGCGAAAGATAGCTCCCGATCACGGCCCGTCTTGCTCGGGCTGGACCGCTTGGAGACCATGGGACTCGTC
CGTGAGCTTGCCGGGTCCCG

Product: putative soluble lytic transglycosylase (SLT) signal peptide

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 781; Mature: 780

Protein sequence:

>781_residues
MSARAAVLRSTALSMSLSASLAAGLTLGLALGGDIALAKDKVPLPKARPIARSMIPATPARDTAESRSKVDARAEAKADV
KADLRDTKSIGKGAAEPSRGAPRDTTPGAVPGSAPSAMPSALPPRQHAALPAPRKPIATAAIAATASTSQSDKDALENVI
ELVRKRKPDDATQVEQAISDPVARKLAEWVILRSDNNNATVERYRAFITANPSWPSQSFLRRRLEAALWDDKRDDSVVWS
WFENESPLSAKGKFALARVMLNRGDRANAERLVREAWRNDAMSEETENNILDQFGALISGGDHKARMDTLLYGSEHEAAL
RAAKRLGSGHMALAKARIAAYRKAANSRALLDAVPHELHGDAGYIFSKIQLLRRDENFTEAAQLMLSAPKDSGRLHNLDE
WWIERRLLARKMIDTGDYRSAYLIARDAALPNRDIYKTEQEFTAGWIALRFLKDPATAAQHFARIGVGSVNPTTLARAGY
WQGRAAEAMGRMQEARAAYTSAAEQSTSYYGQLARAKLGLPQLGLNAAPGGRGRGVERLEVVRAVQLLYDLDEGEVAIPI
FADMGDNGDPDVLLGLAEAAGRQSDARAMLLVGKAALNRGLPFDHYAYPVNGIPAFRSIGPEVEQSVIFSIARQESAFNQ
AVVSPAQAYGLMQVTPDAGRYVCKRAGVSFDLNRMKTDPVYNAQLGAAELGGLLEDYRGSYIMTFAAYNAGRGSVKKWIE
RYGDPRDPNVDAVDWVELIPFAETRNYVQRVMENLQVYRARFGGGSRLQIEADLRRGSVVE

Sequences:

>Translated_781_residues
MSARAAVLRSTALSMSLSASLAAGLTLGLALGGDIALAKDKVPLPKARPIARSMIPATPARDTAESRSKVDARAEAKADV
KADLRDTKSIGKGAAEPSRGAPRDTTPGAVPGSAPSAMPSALPPRQHAALPAPRKPIATAAIAATASTSQSDKDALENVI
ELVRKRKPDDATQVEQAISDPVARKLAEWVILRSDNNNATVERYRAFITANPSWPSQSFLRRRLEAALWDDKRDDSVVWS
WFENESPLSAKGKFALARVMLNRGDRANAERLVREAWRNDAMSEETENNILDQFGALISGGDHKARMDTLLYGSEHEAAL
RAAKRLGSGHMALAKARIAAYRKAANSRALLDAVPHELHGDAGYIFSKIQLLRRDENFTEAAQLMLSAPKDSGRLHNLDE
WWIERRLLARKMIDTGDYRSAYLIARDAALPNRDIYKTEQEFTAGWIALRFLKDPATAAQHFARIGVGSVNPTTLARAGY
WQGRAAEAMGRMQEARAAYTSAAEQSTSYYGQLARAKLGLPQLGLNAAPGGRGRGVERLEVVRAVQLLYDLDEGEVAIPI
FADMGDNGDPDVLLGLAEAAGRQSDARAMLLVGKAALNRGLPFDHYAYPVNGIPAFRSIGPEVEQSVIFSIARQESAFNQ
AVVSPAQAYGLMQVTPDAGRYVCKRAGVSFDLNRMKTDPVYNAQLGAAELGGLLEDYRGSYIMTFAAYNAGRGSVKKWIE
RYGDPRDPNVDAVDWVELIPFAETRNYVQRVMENLQVYRARFGGGSRLQIEADLRRGSVVE
>Mature_780_residues
SARAAVLRSTALSMSLSASLAAGLTLGLALGGDIALAKDKVPLPKARPIARSMIPATPARDTAESRSKVDARAEAKADVK
ADLRDTKSIGKGAAEPSRGAPRDTTPGAVPGSAPSAMPSALPPRQHAALPAPRKPIATAAIAATASTSQSDKDALENVIE
LVRKRKPDDATQVEQAISDPVARKLAEWVILRSDNNNATVERYRAFITANPSWPSQSFLRRRLEAALWDDKRDDSVVWSW
FENESPLSAKGKFALARVMLNRGDRANAERLVREAWRNDAMSEETENNILDQFGALISGGDHKARMDTLLYGSEHEAALR
AAKRLGSGHMALAKARIAAYRKAANSRALLDAVPHELHGDAGYIFSKIQLLRRDENFTEAAQLMLSAPKDSGRLHNLDEW
WIERRLLARKMIDTGDYRSAYLIARDAALPNRDIYKTEQEFTAGWIALRFLKDPATAAQHFARIGVGSVNPTTLARAGYW
QGRAAEAMGRMQEARAAYTSAAEQSTSYYGQLARAKLGLPQLGLNAAPGGRGRGVERLEVVRAVQLLYDLDEGEVAIPIF
ADMGDNGDPDVLLGLAEAAGRQSDARAMLLVGKAALNRGLPFDHYAYPVNGIPAFRSIGPEVEQSVIFSIARQESAFNQA
VVSPAQAYGLMQVTPDAGRYVCKRAGVSFDLNRMKTDPVYNAQLGAAELGGLLEDYRGSYIMTFAAYNAGRGSVKKWIER
YGDPRDPNVDAVDWVELIPFAETRNYVQRVMENLQVYRARFGGGSRLQIEADLRRGSVVE

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=145, Percent_Identity=37.2413793103448, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 85033; Mature: 84902

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARAAVLRSTALSMSLSASLAAGLTLGLALGGDIALAKDKVPLPKARPIARSMIPATPA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHCCCCCC
RDTAESRSKVDARAEAKADVKADLRDTKSIGKGAAEPSRGAPRDTTPGAVPGSAPSAMPS
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ALPPRQHAALPAPRKPIATAAIAATASTSQSDKDALENVIELVRKRKPDDATQVEQAISD
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
PVARKLAEWVILRSDNNNATVERYRAFITANPSWPSQSFLRRRLEAALWDDKRDDSVVWS
HHHHHHHHEEEEECCCCCCHHEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
WFENESPLSAKGKFALARVMLNRGDRANAERLVREAWRNDAMSEETENNILDQFGALISG
EECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
GDHKARMDTLLYGSEHEAALRAAKRLGSGHMALAKARIAAYRKAANSRALLDAVPHELHG
CCCHHHHHHHEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHCCHHHCC
DAGYIFSKIQLLRRDENFTEAAQLMLSAPKDSGRLHNLDEWWIERRLLARKMIDTGDYRS
CHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
AYLIARDAALPNRDIYKTEQEFTAGWIALRFLKDPATAAQHFARIGVGSVNPTTLARAGY
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHCCC
WQGRAAEAMGRMQEARAAYTSAAEQSTSYYGQLARAKLGLPQLGLNAAPGGRGRGVERLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHH
VVRAVQLLYDLDEGEVAIPIFADMGDNGDPDVLLGLAEAAGRQSDARAMLLVGKAALNRG
HHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHEEEEHHHCCCCCCCCEEEEEEHHHHHCC
LPFDHYAYPVNGIPAFRSIGPEVEQSVIFSIARQESAFNQAVVSPAQAYGLMQVTPDAGR
CCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCEEECCCHHH
YVCKRAGVSFDLNRMKTDPVYNAQLGAAELGGLLEDYRGSYIMTFAAYNAGRGSVKKWIE
HHHHHCCCCEEHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
RYGDPRDPNVDAVDWVELIPFAETRNYVQRVMENLQVYRARFGGGSRLQIEADLRRGSVV
HCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
E
C
>Mature Secondary Structure 
SARAAVLRSTALSMSLSASLAAGLTLGLALGGDIALAKDKVPLPKARPIARSMIPATPA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHCCCCCC
RDTAESRSKVDARAEAKADVKADLRDTKSIGKGAAEPSRGAPRDTTPGAVPGSAPSAMPS
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ALPPRQHAALPAPRKPIATAAIAATASTSQSDKDALENVIELVRKRKPDDATQVEQAISD
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
PVARKLAEWVILRSDNNNATVERYRAFITANPSWPSQSFLRRRLEAALWDDKRDDSVVWS
HHHHHHHHEEEEECCCCCCHHEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
WFENESPLSAKGKFALARVMLNRGDRANAERLVREAWRNDAMSEETENNILDQFGALISG
EECCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
GDHKARMDTLLYGSEHEAALRAAKRLGSGHMALAKARIAAYRKAANSRALLDAVPHELHG
CCCHHHHHHHEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHCCHHHCC
DAGYIFSKIQLLRRDENFTEAAQLMLSAPKDSGRLHNLDEWWIERRLLARKMIDTGDYRS
CHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
AYLIARDAALPNRDIYKTEQEFTAGWIALRFLKDPATAAQHFARIGVGSVNPTTLARAGY
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHCCC
WQGRAAEAMGRMQEARAAYTSAAEQSTSYYGQLARAKLGLPQLGLNAAPGGRGRGVERLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHH
VVRAVQLLYDLDEGEVAIPIFADMGDNGDPDVLLGLAEAAGRQSDARAMLLVGKAALNRG
HHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHEEEEHHHCCCCCCCCEEEEEEHHHHHCC
LPFDHYAYPVNGIPAFRSIGPEVEQSVIFSIARQESAFNQAVVSPAQAYGLMQVTPDAGR
CCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCEEECCCHHH
YVCKRAGVSFDLNRMKTDPVYNAQLGAAELGGLLEDYRGSYIMTFAAYNAGRGSVKKWIE
HHHHHCCCCEEHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
RYGDPRDPNVDAVDWVELIPFAETRNYVQRVMENLQVYRARFGGGSRLQIEADLRRGSVV
HCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]