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The map label for this gene is hemB [H]

Identifier: 146341352

GI number: 146341352

Start: 4637439

End: 4638500

Strand: Direct

Name: hemB [H]

Synonym: BRADO4436

Alternate gene names: 146341352

Gene position: 4637439-4638500 (Clockwise)

Preceding gene: 146341338

Following gene: 146341353

Centisome position: 62.19

GC content: 66.29

Gene sequence:

>1062_bases
ATGGCGATCAAATTCGGGCGCCCGATCGATGTGCGCGACGTCGCGCGTAACCCCGCCGCGGGAACACCACCGGTGCTCGA
TTTGACCATCCGCCCCCGGCGAAACCGCAAGGCGGAATGGGCCCGCCGCCTGGTCCGCGAAAACGTCCTGACCACCGACG
ATCTGATCTGGCCGCTGTTCGTCGTACAAGGCGACCGGACCCGCAGCACCGTCGCGTCCATGCCCGGCGTCGAGCGGCTG
AGCGTCGACGAGATCGTGCGCGACGCCGAGCGCGCCGCCAAGCTCGATATTCCCTGTATCGCGCTGTTCCCCTACACCGA
GCCGTCGCTGCGCGACGAGCACGGCTCGGAGGCCTGCAATCCCGACAATCTGGTTTGCCAGGCGGTGCGCGCGATCAAGA
AGGAGTTCCCGGAGATCGGCGTGCTCTGCGACGTGGCGCTGGACCCGTTTACCAGCCATGGCCATGACGGGCTGATCAAG
GACGGCCGCATCCTGAATGACGAGACCGTCGCGGTGCTGGTGCGCCAGGCGCTGGTTCAGGCCGAGGCCGGCTGCGACAT
CATCGCGCCATCGGACATGATGGACGGCCGCGTTGGCGCGATCCGTCAGGCGCTGGACCAGTCGGGCTTCCTCGACGTGC
AGATCATGGCTTATGCGGCCAAATATGCCTCCGCCTTCTACGGCCCGTTCCGCGACGCGATCGGCTCGGCCAAGACGCTG
ACCGGCGACAAGCGCACCTACCAGATGGACAGCGCCAACACCGATGAGGCGCTGCGCGAGGTCGAGCTCGACATCGCCGA
AGGCGCCGACATGGTGATGGTGAAGCCGGGCATGCCCTATCTCGACGTCGTCCGCCGCGTGAAGGACACCTTCAGCATGC
CGACCTTCGCCTATCAGGTATCGGGCGAATACGCGATGATTGCCGCGGCCGCCAACAATGGCTGGATCGACGGTGACCGG
GCCATCATGGAGAGCCTGCTGGCTTTCAAGCGCGCCGGCGCAGATGGTGTCCTCACCTATTTCGCGCCGCAGGCCGCCGA
GCGGTTGCGCCGGCAGAGCTAA

Upstream 100 bases:

>100_bases
GCGTCAGGTCAAGTGACGTGCGGCGTCCAAAGTGCTACACATTGTTGGAATACCAACAAATCAATCCGGCTCACCGGCCA
GCTCAAGGAGCCAGCACCCC

Downstream 100 bases:

>100_bases
TCCCCGGACACGTCGCGGCGGACATTGACCCCGCCGCGAGGCTCGCCGCCGTCAGAAAATCGCGAGACCGCCGAAAATTT
GCCGCGGGCGTTGCAAGGAT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MAIKFGRPIDVRDVARNPAAGTPPVLDLTIRPRRNRKAEWARRLVRENVLTTDDLIWPLFVVQGDRTRSTVASMPGVERL
SVDEIVRDAERAAKLDIPCIALFPYTEPSLRDEHGSEACNPDNLVCQAVRAIKKEFPEIGVLCDVALDPFTSHGHDGLIK
DGRILNDETVAVLVRQALVQAEAGCDIIAPSDMMDGRVGAIRQALDQSGFLDVQIMAYAAKYASAFYGPFRDAIGSAKTL
TGDKRTYQMDSANTDEALREVELDIAEGADMVMVKPGMPYLDVVRRVKDTFSMPTFAYQVSGEYAMIAAAANNGWIDGDR
AIMESLLAFKRAGADGVLTYFAPQAAERLRRQS

Sequences:

>Translated_353_residues
MAIKFGRPIDVRDVARNPAAGTPPVLDLTIRPRRNRKAEWARRLVRENVLTTDDLIWPLFVVQGDRTRSTVASMPGVERL
SVDEIVRDAERAAKLDIPCIALFPYTEPSLRDEHGSEACNPDNLVCQAVRAIKKEFPEIGVLCDVALDPFTSHGHDGLIK
DGRILNDETVAVLVRQALVQAEAGCDIIAPSDMMDGRVGAIRQALDQSGFLDVQIMAYAAKYASAFYGPFRDAIGSAKTL
TGDKRTYQMDSANTDEALREVELDIAEGADMVMVKPGMPYLDVVRRVKDTFSMPTFAYQVSGEYAMIAAAANNGWIDGDR
AIMESLLAFKRAGADGVLTYFAPQAAERLRRQS
>Mature_352_residues
AIKFGRPIDVRDVARNPAAGTPPVLDLTIRPRRNRKAEWARRLVRENVLTTDDLIWPLFVVQGDRTRSTVASMPGVERLS
VDEIVRDAERAAKLDIPCIALFPYTEPSLRDEHGSEACNPDNLVCQAVRAIKKEFPEIGVLCDVALDPFTSHGHDGLIKD
GRILNDETVAVLVRQALVQAEAGCDIIAPSDMMDGRVGAIRQALDQSGFLDVQIMAYAAKYASAFYGPFRDAIGSAKTLT
GDKRTYQMDSANTDEALREVELDIAEGADMVMVKPGMPYLDVVRRVKDTFSMPTFAYQVSGEYAMIAAAANNGWIDGDRA
IMESLLAFKRAGADGVLTYFAPQAAERLRRQS

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=308, Percent_Identity=42.5324675324675, Blast_Score=244, Evalue=1e-64,
Organism=Escherichia coli, GI87081728, Length=322, Percent_Identity=46.583850931677, Blast_Score=264, Evalue=8e-72,
Organism=Saccharomyces cerevisiae, GI6321398, Length=317, Percent_Identity=36.9085173501577, Blast_Score=193, Evalue=4e-50,
Organism=Drosophila melanogaster, GI21358291, Length=295, Percent_Identity=41.0169491525424, Blast_Score=248, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 38810; Mature: 38679

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKFGRPIDVRDVARNPAAGTPPVLDLTIRPRRNRKAEWARRLVRENVLTTDDLIWPLF
CCCCCCCCCCHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHEEEE
VVQGDRTRSTVASMPGVERLSVDEIVRDAERAAKLDIPCIALFPYTEPSLRDEHGSEACN
EEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCC
PDNLVCQAVRAIKKEFPEIGVLCDVALDPFTSHGHDGLIKDGRILNDETVAVLVRQALVQ
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHH
AEAGCDIIAPSDMMDGRVGAIRQALDQSGFLDVQIMAYAAKYASAFYGPFRDAIGSAKTL
HHCCCCEECCCHHCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TGDKRTYQMDSANTDEALREVELDIAEGADMVMVKPGMPYLDVVRRVKDTFSMPTFAYQV
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEE
SGEYAMIAAAANNGWIDGDRAIMESLLAFKRAGADGVLTYFAPQAAERLRRQS
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHCHHHHHHHHCCC
>Mature Secondary Structure 
AIKFGRPIDVRDVARNPAAGTPPVLDLTIRPRRNRKAEWARRLVRENVLTTDDLIWPLF
CCCCCCCCCHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHEEEE
VVQGDRTRSTVASMPGVERLSVDEIVRDAERAAKLDIPCIALFPYTEPSLRDEHGSEACN
EEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCC
PDNLVCQAVRAIKKEFPEIGVLCDVALDPFTSHGHDGLIKDGRILNDETVAVLVRQALVQ
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHH
AEAGCDIIAPSDMMDGRVGAIRQALDQSGFLDVQIMAYAAKYASAFYGPFRDAIGSAKTL
HHCCCCEECCCHHCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TGDKRTYQMDSANTDEALREVELDIAEGADMVMVKPGMPYLDVVRRVKDTFSMPTFAYQV
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEE
SGEYAMIAAAANNGWIDGDRAIMESLLAFKRAGADGVLTYFAPQAAERLRRQS
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]