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The map label for this gene is amiD [H]

Identifier: 146341325

GI number: 146341325

Start: 4608641

End: 4610041

Strand: Direct

Name: amiD [H]

Synonym: BRADO4407

Alternate gene names: 146341325

Gene position: 4608641-4610041 (Clockwise)

Preceding gene: 146341316

Following gene: 146341337

Centisome position: 61.81

GC content: 66.02

Gene sequence:

>1401_bases
ATGCCAGCAACAGATCTCCACTATCTCGGACTCGTTGATGTCGGTCGGAAGATCCAGGCTAAGGAGTTGTCGCCGGTCGA
AGTGACCAAGGCCATGCTCGAGCGGATCGAGAAGCTCGATGGCCAACTCAAGAGCTTCGCCTATGTCATGGCCGACTCGG
CCGTCGCGGACGCTGCCGCCGCTGAGAAGGAGATTGCCTCCGGCAAGATCAGGGGCCCGTTGCACGGCGTGCCCGTCGCG
GTGAAGGATCTCTGCTGGGCCAAGGGCGCGCCAGCGGCGCACGGCATGACGATCCATCGTGACTTCCGTCCGAACGAGGA
CGCGACAGTGGTCGCGCGGCTGAAGGCCGCCGGCGCTGTGATCCTCGGCAAGCTGCAGCAGACCGAAGGGGCTTACGCCG
ATCATCATCCGAAGATCGATCCACCCAAGAATCCCTGGAACGCCGATCTTTGGCCTGGCGCCTCCTCGAGCGGCTCTGGC
GCCGCCACCGCGGCGGGCCTGTGCTTCGGCTCGCTCGGCACCGACACCGGCGGCTCGATCCGCTTTCCCTCCGCGGCCAA
TGGCGTCACCGGGCTTAAGCCGACCTGGGGACGGGTCAGCCGCTACGGCGCCTTCGAACTCGCGGCGACGCTGGACCACA
TCGGCCCCATGGCGCGCAGCGCGGCTGATTGCGGCGCCGTCCTCGGCGTGATCGCAGGGCAAGATCCCAGGGATACGACG
GCTGTCCCGCTGCCCGTGCCTGACTATCTGGCCAGTCTCTCCGGCGACCTCCGGGGCGTGACGATCGGGATCGACCGGCG
CTGGACCAGCGAAGGCACCGACAGCGATACAGCCAACGTCCTGACCGAGGCTCTGCGTGTGGCTGTCGATCTCGGCGCAC
AGATCAAGGAGATCAACTTTCCCGATTCCCAGGCGGTCATCGACGATTGGTTCCCGCTTTGCGGCATCGAGGCAGCGGTC
GCGCACGAAAACACCTACCCGGCGCGCAAGGACGAGTACGGTCCTGGGCTCGCCGGCCTGCTGGATCTCGGCCACCAACA
ATCAGGTATGGACTATCAGAAAATCGTGCTTCGGCGCGAGGCGTTCCGCGGGGCAGTCCGCGTACTGTTCGAGACGGTCG
ACCTCCTTGCGGTCCCGGCGCAGGCCTTTGCTGCACCGAGCCTCGCCAAGATGGCGACGCTTGGCGAGGACGCTTCGCTG
ATCGGCGGACTGCTTCGTTTCACCTGCCCGTTCGACATGACGGGAAGCCCGACCGTGACGTTGCCCGGAGGCTTTGCCGC
GAATGGCGGTCCCGTGGGCTTCCAGTTCGTCGGCCGTCATTTCGACGAGGCCGGCCTCGTGCGCGCCGGCGATGCGTTTC
AGCGCGCCACCGACTGGCACAAACGTCATCCCGGCATCTGA

Upstream 100 bases:

>100_bases
GTTTGCCGCCCCGGCGAGCTTGCGATGCGTCCGCGTCGCCGAAACCGTGGTGCATGACCGAGTGCAAATATGCACCATAT
GACGAGAGAGGATGACGAGG

Downstream 100 bases:

>100_bases
GTTGAGGGGCCGATCATCGATTCGCAGATTTGCGGAGAGCCGATCGTGGCTGCGCAATAGCCCGGGACGCCAGGACCACC
CCACTTCGCCCTCCCCTCAC

Product: putative amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MPATDLHYLGLVDVGRKIQAKELSPVEVTKAMLERIEKLDGQLKSFAYVMADSAVADAAAAEKEIASGKIRGPLHGVPVA
VKDLCWAKGAPAAHGMTIHRDFRPNEDATVVARLKAAGAVILGKLQQTEGAYADHHPKIDPPKNPWNADLWPGASSSGSG
AATAAGLCFGSLGTDTGGSIRFPSAANGVTGLKPTWGRVSRYGAFELAATLDHIGPMARSAADCGAVLGVIAGQDPRDTT
AVPLPVPDYLASLSGDLRGVTIGIDRRWTSEGTDSDTANVLTEALRVAVDLGAQIKEINFPDSQAVIDDWFPLCGIEAAV
AHENTYPARKDEYGPGLAGLLDLGHQQSGMDYQKIVLRREAFRGAVRVLFETVDLLAVPAQAFAAPSLAKMATLGEDASL
IGGLLRFTCPFDMTGSPTVTLPGGFAANGGPVGFQFVGRHFDEAGLVRAGDAFQRATDWHKRHPGI

Sequences:

>Translated_466_residues
MPATDLHYLGLVDVGRKIQAKELSPVEVTKAMLERIEKLDGQLKSFAYVMADSAVADAAAAEKEIASGKIRGPLHGVPVA
VKDLCWAKGAPAAHGMTIHRDFRPNEDATVVARLKAAGAVILGKLQQTEGAYADHHPKIDPPKNPWNADLWPGASSSGSG
AATAAGLCFGSLGTDTGGSIRFPSAANGVTGLKPTWGRVSRYGAFELAATLDHIGPMARSAADCGAVLGVIAGQDPRDTT
AVPLPVPDYLASLSGDLRGVTIGIDRRWTSEGTDSDTANVLTEALRVAVDLGAQIKEINFPDSQAVIDDWFPLCGIEAAV
AHENTYPARKDEYGPGLAGLLDLGHQQSGMDYQKIVLRREAFRGAVRVLFETVDLLAVPAQAFAAPSLAKMATLGEDASL
IGGLLRFTCPFDMTGSPTVTLPGGFAANGGPVGFQFVGRHFDEAGLVRAGDAFQRATDWHKRHPGI
>Mature_465_residues
PATDLHYLGLVDVGRKIQAKELSPVEVTKAMLERIEKLDGQLKSFAYVMADSAVADAAAAEKEIASGKIRGPLHGVPVAV
KDLCWAKGAPAAHGMTIHRDFRPNEDATVVARLKAAGAVILGKLQQTEGAYADHHPKIDPPKNPWNADLWPGASSSGSGA
ATAAGLCFGSLGTDTGGSIRFPSAANGVTGLKPTWGRVSRYGAFELAATLDHIGPMARSAADCGAVLGVIAGQDPRDTTA
VPLPVPDYLASLSGDLRGVTIGIDRRWTSEGTDSDTANVLTEALRVAVDLGAQIKEINFPDSQAVIDDWFPLCGIEAAVA
HENTYPARKDEYGPGLAGLLDLGHQQSGMDYQKIVLRREAFRGAVRVLFETVDLLAVPAQAFAAPSLAKMATLGEDASLI
GGLLRFTCPFDMTGSPTVTLPGGFAANGGPVGFQFVGRHFDEAGLVRAGDAFQRATDWHKRHPGI

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=502, Percent_Identity=29.2828685258964, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI195972892, Length=489, Percent_Identity=25.3578732106339, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI166795287, Length=251, Percent_Identity=30.6772908366534, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=411, Percent_Identity=27.2506082725061, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17537465, Length=249, Percent_Identity=28.9156626506024, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556264, Length=311, Percent_Identity=27.0096463022508, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=210, Percent_Identity=29.0476190476191, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=466, Percent_Identity=23.8197424892704, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319685, Length=401, Percent_Identity=26.4339152119701, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320448, Length=225, Percent_Identity=31.5555555555556, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648113, Length=477, Percent_Identity=31.6561844863732, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI161078093, Length=488, Percent_Identity=25, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24652985, Length=248, Percent_Identity=30.6451612903226, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922090, Length=248, Percent_Identity=30.6451612903226, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652981, Length=248, Percent_Identity=30.6451612903226, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652983, Length=248, Percent_Identity=30.6451612903226, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356731, Length=233, Percent_Identity=29.6137339055794, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24644968, Length=253, Percent_Identity=26.8774703557312, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI45550774, Length=263, Percent_Identity=27.7566539923954, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648435, Length=263, Percent_Identity=27.7566539923954, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648437, Length=263, Percent_Identity=27.7566539923954, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648441, Length=182, Percent_Identity=31.3186813186813, Blast_Score=65, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24648439, Length=182, Percent_Identity=31.3186813186813, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 48960; Mature: 48829

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATDLHYLGLVDVGRKIQAKELSPVEVTKAMLERIEKLDGQLKSFAYVMADSAVADAAA
CCCCCCEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKEIASGKIRGPLHGVPVAVKDLCWAKGAPAAHGMTIHRDFRPNEDATVVARLKAAGAV
HHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEHHHHCCHH
ILGKLQQTEGAYADHHPKIDPPKNPWNADLWPGASSSGSGAATAAGLCFGSLGTDTGGSI
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCE
RFPSAANGVTGLKPTWGRVSRYGAFELAATLDHIGPMARSAADCGAVLGVIAGQDPRDTT
ECCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AVPLPVPDYLASLSGDLRGVTIGIDRRWTSEGTDSDTANVLTEALRVAVDLGAQIKEINF
EECCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCC
PDSQAVIDDWFPLCGIEAAVAHENTYPARKDEYGPGLAGLLDLGHQQSGMDYQKIVLRRE
CCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHH
AFRGAVRVLFETVDLLAVPAQAFAAPSLAKMATLGEDASLIGGLLRFTCPFDMTGSPTVT
HHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEE
LPGGFAANGGPVGFQFVGRHFDEAGLVRAGDAFQRATDWHKRHPGI
ECCCCCCCCCCCCHHHHHHHCCCCCCEECCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PATDLHYLGLVDVGRKIQAKELSPVEVTKAMLERIEKLDGQLKSFAYVMADSAVADAAA
CCCCCEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKEIASGKIRGPLHGVPVAVKDLCWAKGAPAAHGMTIHRDFRPNEDATVVARLKAAGAV
HHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEHHHHCCHH
ILGKLQQTEGAYADHHPKIDPPKNPWNADLWPGASSSGSGAATAAGLCFGSLGTDTGGSI
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCE
RFPSAANGVTGLKPTWGRVSRYGAFELAATLDHIGPMARSAADCGAVLGVIAGQDPRDTT
ECCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AVPLPVPDYLASLSGDLRGVTIGIDRRWTSEGTDSDTANVLTEALRVAVDLGAQIKEINF
EECCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCC
PDSQAVIDDWFPLCGIEAAVAHENTYPARKDEYGPGLAGLLDLGHQQSGMDYQKIVLRRE
CCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHH
AFRGAVRVLFETVDLLAVPAQAFAAPSLAKMATLGEDASLIGGLLRFTCPFDMTGSPTVT
HHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEE
LPGGFAANGGPVGFQFVGRHFDEAGLVRAGDAFQRATDWHKRHPGI
ECCCCCCCCCCCCHHHHHHHCCCCCCEECCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]