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The map label for this gene is degA [H]

Identifier: 146341315

GI number: 146341315

Start: 4595333

End: 4596316

Strand: Direct

Name: degA [H]

Synonym: BRADO4397

Alternate gene names: 146341315

Gene position: 4595333-4596316 (Clockwise)

Preceding gene: 146341290

Following gene: 146341316

Centisome position: 61.63

GC content: 69.61

Gene sequence:

>984_bases
ATGCCCGACGACGTCTCACTTGCCACCGTTGCCGTCAAAGCCGGCGTGTCGGTTTCGACCGTGTCGCGGATCGTCAACGG
GCAGACGCACCGCGCCTCGGCGGCGACGGTGATGCGGGTGCGCGAGGCGATCGCGGCGGTCGGCTATCGGCCGAACCCGG
TCGGTCGGGCGCTCAAGCGCCGCCAGAGCCAGCTGGTGGCGATGATCGCGGCCAATCTCGACAACCCTGCGATGGCCACC
ATCGCGAGCTCCACGGAAGCCGCGCTGCGCGAGGCCGGCTATGTCATGGTGCTGTGCGACACCCATGACCGGCCGGAGCT
GCAGGACGAATATCTCGCGGCAATGCGAGCGCAGATGGTGAGCGGCTACGTCCTGGTTGCGGCAGTGCCGAGCCCCGGCC
TCGCCGAGCTGTCGGCATCGGGCGCGCCGGCCGTGTTCGTGAACCGCAGGAATCCGCAGGGCGCCGGCCCGTTCGTCGGC
ATCGACGATCGCGCTGCGGGCGCCGCGGTCGCCGATCATTTCGCCGATCGCGGCCTGCGGAGGCCGGGCGTGGTGTTTCC
GCGGCTGGGCTCTTCGGCCTCGCGCGACCGCGTCGCCGGCTTCCTCGCGCGGCTGCAGGCGCGCGGCTATGGCGATGACG
CGATCATCCGCGAAGGCGGCGACGGACGGTCGCATCTCGAAATCGGCTACGATGCCGCCGCGCGCGTGCTCGCGCGGAGC
CAGAGGCCGGACGCCCTGCTCTGCGTCAGCGACCAGATCGCCTATGGCGTGAACCGCCGCGCCCGCGAGCGCGGCCTGAC
CATTCCTGCGGATTGCGATCTTGTCAGTATCGACGGCACGCCGCTCAACCGATGGGTCGCGCCGTGGCTGACATCTGTCG
AGATTCCTTACGCGATCTACGGCGCCGAAATCGTCGCCGGCCTGCAAATGCTATGGCAGGGCGGAACGTTCGGCGAGCGC
CTGCTTGCCTACAGGTTCGCGTAA

Upstream 100 bases:

>100_bases
GTCAAGCCTGGTCTAGCCTGCGCCCGAGAGCTGATGCCGGCGCGTCGAGCGCCCGACGGCTGACGCCAATGACAGGCGAA
ACCGGAGCTGACGGAGAGCG

Downstream 100 bases:

>100_bases
GGCGCGGCCGGCCGCCGCAAAGGTTCGCCCTGCCTCTGCGATTGTTCACATCTGACGCGCGCCGGCATCGACGCGGAGAT
CGGCGGTGCTGGCATGATAT

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPDDVSLATVAVKAGVSVSTVSRIVNGQTHRASAATVMRVREAIAAVGYRPNPVGRALKRRQSQLVAMIAANLDNPAMAT
IASSTEAALREAGYVMVLCDTHDRPELQDEYLAAMRAQMVSGYVLVAAVPSPGLAELSASGAPAVFVNRRNPQGAGPFVG
IDDRAAGAAVADHFADRGLRRPGVVFPRLGSSASRDRVAGFLARLQARGYGDDAIIREGGDGRSHLEIGYDAAARVLARS
QRPDALLCVSDQIAYGVNRRARERGLTIPADCDLVSIDGTPLNRWVAPWLTSVEIPYAIYGAEIVAGLQMLWQGGTFGER
LLAYRFA

Sequences:

>Translated_327_residues
MPDDVSLATVAVKAGVSVSTVSRIVNGQTHRASAATVMRVREAIAAVGYRPNPVGRALKRRQSQLVAMIAANLDNPAMAT
IASSTEAALREAGYVMVLCDTHDRPELQDEYLAAMRAQMVSGYVLVAAVPSPGLAELSASGAPAVFVNRRNPQGAGPFVG
IDDRAAGAAVADHFADRGLRRPGVVFPRLGSSASRDRVAGFLARLQARGYGDDAIIREGGDGRSHLEIGYDAAARVLARS
QRPDALLCVSDQIAYGVNRRARERGLTIPADCDLVSIDGTPLNRWVAPWLTSVEIPYAIYGAEIVAGLQMLWQGGTFGER
LLAYRFA
>Mature_326_residues
PDDVSLATVAVKAGVSVSTVSRIVNGQTHRASAATVMRVREAIAAVGYRPNPVGRALKRRQSQLVAMIAANLDNPAMATI
ASSTEAALREAGYVMVLCDTHDRPELQDEYLAAMRAQMVSGYVLVAAVPSPGLAELSASGAPAVFVNRRNPQGAGPFVGI
DDRAAGAAVADHFADRGLRRPGVVFPRLGSSASRDRVAGFLARLQARGYGDDAIIREGGDGRSHLEIGYDAAARVLARSQ
RPDALLCVSDQIAYGVNRRARERGLTIPADCDLVSIDGTPLNRWVAPWLTSVEIPYAIYGAEIVAGLQMLWQGGTFGERL
LAYRFA

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=320, Percent_Identity=27.5, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787948, Length=298, Percent_Identity=28.1879194630872, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=24.9146757679181, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790194, Length=296, Percent_Identity=25.3378378378378, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1789456, Length=320, Percent_Identity=27.1875, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1789068, Length=319, Percent_Identity=25.0783699059561, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787580, Length=313, Percent_Identity=24.9201277955272, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788474, Length=297, Percent_Identity=24.9158249158249, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786540, Length=327, Percent_Identity=28.7461773700306, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790689, Length=315, Percent_Identity=24.7619047619048, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI48994940, Length=308, Percent_Identity=23.7012987012987, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787906, Length=256, Percent_Identity=26.953125, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790715, Length=293, Percent_Identity=20.4778156996587, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34753; Mature: 34622

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDDVSLATVAVKAGVSVSTVSRIVNGQTHRASAATVMRVREAIAAVGYRPNPVGRALKR
CCCCCCEEEEHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RQSQLVAMIAANLDNPAMATIASSTEAALREAGYVMVLCDTHDRPELQDEYLAAMRAQMV
HHHHHHHHHHHCCCCCHHEEECCHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHH
SGYVLVAAVPSPGLAELSASGAPAVFVNRRNPQGAGPFVGIDDRAAGAAVADHFADRGLR
CCEEEEEECCCCCHHHHCCCCCCEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHCCCC
RPGVVFPRLGSSASRDRVAGFLARLQARGYGDDAIIREGGDGRSHLEIGYDAAARVLARS
CCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEECHHHHHHHHHHC
QRPDALLCVSDQIAYGVNRRARERGLTIPADCDLVSIDGTPLNRWVAPWLTSVEIPYAIY
CCCCEEEEECCHHHHCCCHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCHHHH
GAEIVAGLQMLWQGGTFGERLLAYRFA
HHHHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure 
PDDVSLATVAVKAGVSVSTVSRIVNGQTHRASAATVMRVREAIAAVGYRPNPVGRALKR
CCCCCEEEEHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RQSQLVAMIAANLDNPAMATIASSTEAALREAGYVMVLCDTHDRPELQDEYLAAMRAQMV
HHHHHHHHHHHCCCCCHHEEECCHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHH
SGYVLVAAVPSPGLAELSASGAPAVFVNRRNPQGAGPFVGIDDRAAGAAVADHFADRGLR
CCEEEEEECCCCCHHHHCCCCCCEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHCCCC
RPGVVFPRLGSSASRDRVAGFLARLQARGYGDDAIIREGGDGRSHLEIGYDAAARVLARS
CCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEECHHHHHHHHHHC
QRPDALLCVSDQIAYGVNRRARERGLTIPADCDLVSIDGTPLNRWVAPWLTSVEIPYAIY
CCCCEEEEECCHHHHCCCHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCHHHH
GAEIVAGLQMLWQGGTFGERLLAYRFA
HHHHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]