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The map label for this gene is mmoX [H]

Identifier: 146341273

GI number: 146341273

Start: 4541119

End: 4542789

Strand: Reverse

Name: mmoX [H]

Synonym: BRADO4350

Alternate gene names: 146341273

Gene position: 4542789-4541119 (Counterclockwise)

Preceding gene: 146341274

Following gene: 146341272

Centisome position: 60.92

GC content: 62.12

Gene sequence:

>1671_bases
ATGTCTGCAAGTCTGACGCTCAACAAGATTACGGCACAGAAGGGCATTACGATCGCCGAGGCAGCTAGTCGCGTCGCTGA
TCTCGGCTGGACGCCGAGCTATGTCCAGGAAGCGATGACGTTCCCGACGGACTACAAGATTTCCAAGACTCCCCGCGATC
CGATGAAGCAGGTCCTGCGCTCCTATTTCCCGATGCAGGAGGAGAAGGACAACCGCGTCTACGGCGCGCTCGACGCCGCC
TTGCGCGGCGACATGTTCCGCAATGTCGAGCCGCGCTGGGTCGAATGGATGAAGCTGTTCCTGGCCATCATTCCGTTCCC
GGAGATCTCGGCGGCGCGATCGATGGCGATGGTCGGCCGGCTGGCGCCGGGCGAAGAGCTGCGCACCGGCTTCACAATGC
AGATGGTCGACGAGTTCCGGCACTCGACGATCCAGATGAACCTCAAGAAGTGGTACATGGAGAATTACATCGATCCGGCC
GGGTTCGACATCACCGAGGCCGCGTTCGGCAAGTGCTACGCCACCACGATCGGGCGGCAGTTCGCCGAGGGCTTTCTCAC
CGGCGACGCGATCACCGCGGCCAACGTGTACCTGACGGTGGTGGCCGAGACCGCGTTCACCAACACGCTGTTCGTGGCGA
TGCCGTCGGAGGCCGCGCGCAACGGCGACTATGCGCTGCCGACGGTGTTCCTGTCGGTGCAGTCGGATGAATCGCGTCAC
ATCGGCAACGGCCATTCGATGCTGATGGCGATGATCAACGATCCGTCGAACCACCAGCTGCTCGAGCGCGACCTTAAATA
TGCGTTCTGGCAGAACCATGCCATCGTCGACGCCGCGATCGGTACCTTCATCGAATACGGCACCACCAACCGCGACAAGA
ACAAGGAGTCCTATGCGGAGCTGTGGCACCGCTGGATCTATGAAGACTACTACCGGACCTACATGCTGCCGCTGGAGAAG
TACGGCATCAAGATCCACCATGACGATGTCGCCGCCGCCTGGGACCGAATCGTCAAGAAGAACTACGTCCACAAGACCGC
CCAGTTCTTCGCGGTCGGCTGGTCCGCGAACTTCTGGCGAATCGAGGCCCAGACCGAGAAGGACTTCGAGTGGTTCGAGC
ACAAGTACCCCGGCTGGTACGCCGAGTTCGGTGACTTCTGGAAGTGGTATGCGAAGCTGAGCGTGCCGGGCGAGACCAAC
ATCCTGTTCAACAGCGACGTGGGTTACGTGTACCCGCATCGGTGCTGGAGCTGCATGGTGCCTTGCCTGATCCGCGAGGA
CTTCGTCTGCGACGAGGTCGACGGCAAGATCTACACCTACTGCTCGGAAGGCTGCCGCTGGACCCACAAGGTGGCGTTCG
CCGCCGAGTATGAGGGCCGACCCACGCCTGCGATGGGCCGCTTCAGCGGTCGCCGCGAGTGGGAGGACTGCTACCACGGC
TGGGACATCGCCGACGCCATCAAGGATCTCGGCTTCGTCCGTCCGGATGGCAAGACCCTGACCGCCCAGCCGCATCTGCG
GTTCGACGCCAAGGACATGTGGACGCTCGATCACGTGCGCGGCCATCAGCTCGGCAGCCCGCTGCGGGGCTTCCGCGCGC
TCTCGCCGATCGAGCGCGAGATCGCAGTCGCCGATTATCGCAAGGGCTTCACCATCAATCCTTGCCATTGA

Upstream 100 bases:

>100_bases
CAGGAGACGTCGCGGGAGCCGTCCTTGATCGGAGGCTCCTCCGACCGAACGATCAAGGCCTGACGACAACAAGCTGATGC
AACGTTCAAAGGAGAAGGAA

Downstream 100 bases:

>100_bases
CGCTGCGAGAGCAGGGGGCCGGTTCGCCGGCTCCCTGCGACCATCTGATCAAGGGTGCCGGGGAGGATGTCGATGTCGGT
TGGGACCAAGGCTGCCGTGA

Product: putative methane/phenol/toluene monooxygenase subunit alpha

Products: NA

Alternate protein names: Methane hydroxylase [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSASLTLNKITAQKGITIAEAASRVADLGWTPSYVQEAMTFPTDYKISKTPRDPMKQVLRSYFPMQEEKDNRVYGALDAA
LRGDMFRNVEPRWVEWMKLFLAIIPFPEISAARSMAMVGRLAPGEELRTGFTMQMVDEFRHSTIQMNLKKWYMENYIDPA
GFDITEAAFGKCYATTIGRQFAEGFLTGDAITAANVYLTVVAETAFTNTLFVAMPSEAARNGDYALPTVFLSVQSDESRH
IGNGHSMLMAMINDPSNHQLLERDLKYAFWQNHAIVDAAIGTFIEYGTTNRDKNKESYAELWHRWIYEDYYRTYMLPLEK
YGIKIHHDDVAAAWDRIVKKNYVHKTAQFFAVGWSANFWRIEAQTEKDFEWFEHKYPGWYAEFGDFWKWYAKLSVPGETN
ILFNSDVGYVYPHRCWSCMVPCLIREDFVCDEVDGKIYTYCSEGCRWTHKVAFAAEYEGRPTPAMGRFSGRREWEDCYHG
WDIADAIKDLGFVRPDGKTLTAQPHLRFDAKDMWTLDHVRGHQLGSPLRGFRALSPIEREIAVADYRKGFTINPCH

Sequences:

>Translated_556_residues
MSASLTLNKITAQKGITIAEAASRVADLGWTPSYVQEAMTFPTDYKISKTPRDPMKQVLRSYFPMQEEKDNRVYGALDAA
LRGDMFRNVEPRWVEWMKLFLAIIPFPEISAARSMAMVGRLAPGEELRTGFTMQMVDEFRHSTIQMNLKKWYMENYIDPA
GFDITEAAFGKCYATTIGRQFAEGFLTGDAITAANVYLTVVAETAFTNTLFVAMPSEAARNGDYALPTVFLSVQSDESRH
IGNGHSMLMAMINDPSNHQLLERDLKYAFWQNHAIVDAAIGTFIEYGTTNRDKNKESYAELWHRWIYEDYYRTYMLPLEK
YGIKIHHDDVAAAWDRIVKKNYVHKTAQFFAVGWSANFWRIEAQTEKDFEWFEHKYPGWYAEFGDFWKWYAKLSVPGETN
ILFNSDVGYVYPHRCWSCMVPCLIREDFVCDEVDGKIYTYCSEGCRWTHKVAFAAEYEGRPTPAMGRFSGRREWEDCYHG
WDIADAIKDLGFVRPDGKTLTAQPHLRFDAKDMWTLDHVRGHQLGSPLRGFRALSPIEREIAVADYRKGFTINPCH
>Mature_555_residues
SASLTLNKITAQKGITIAEAASRVADLGWTPSYVQEAMTFPTDYKISKTPRDPMKQVLRSYFPMQEEKDNRVYGALDAAL
RGDMFRNVEPRWVEWMKLFLAIIPFPEISAARSMAMVGRLAPGEELRTGFTMQMVDEFRHSTIQMNLKKWYMENYIDPAG
FDITEAAFGKCYATTIGRQFAEGFLTGDAITAANVYLTVVAETAFTNTLFVAMPSEAARNGDYALPTVFLSVQSDESRHI
GNGHSMLMAMINDPSNHQLLERDLKYAFWQNHAIVDAAIGTFIEYGTTNRDKNKESYAELWHRWIYEDYYRTYMLPLEKY
GIKIHHDDVAAAWDRIVKKNYVHKTAQFFAVGWSANFWRIEAQTEKDFEWFEHKYPGWYAEFGDFWKWYAKLSVPGETNI
LFNSDVGYVYPHRCWSCMVPCLIREDFVCDEVDGKIYTYCSEGCRWTHKVAFAAEYEGRPTPAMGRFSGRREWEDCYHGW
DIADAIKDLGFVRPDGKTLTAQPHLRFDAKDMWTLDHVRGHQLGSPLRGFRALSPIEREIAVADYRKGFTINPCH

Specific function: Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR003430
- InterPro:   IPR012348 [H]

Pfam domain/function: PF02332 Phenol_Hydrox [H]

EC number: =1.14.13.25 [H]

Molecular weight: Translated: 64103; Mature: 63972

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASLTLNKITAQKGITIAEAASRVADLGWTPSYVQEAMTFPTDYKISKTPRDPMKQVLR
CCCCEEEHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCCCHHHHHHHHH
SYFPMQEEKDNRVYGALDAALRGDMFRNVEPRWVEWMKLFLAIIPFPEISAARSMAMVGR
HHCCCCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
LAPGEELRTGFTMQMVDEFRHSTIQMNLKKWYMENYIDPAGFDITEAAFGKCYATTIGRQ
CCCCHHHHCCCHHHHHHHHHHHHEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
FAEGFLTGDAITAANVYLTVVAETAFTNTLFVAMPSEAARNGDYALPTVFLSVQSDESRH
HHHHHCCCCCEECCEEEEEEEEHHHCCCEEEEEECCHHHCCCCEECEEEEEEEECCCCCC
IGNGHSMLMAMINDPSNHQLLERDLKYAFWQNHAIVDAAIGTFIEYGTTNRDKNKESYAE
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
LWHRWIYEDYYRTYMLPLEKYGIKIHHDDVAAAWDRIVKKNYVHKTAQFFAVGWSANFWR
HHHHHHHHHHHHHHEEEHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEE
IEAQTEKDFEWFEHKYPGWYAEFGDFWKWYAKLSVPGETNILFNSDVGYVYPHRCWSCMV
EEECCCCCHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCCEEEECCCCCCCHHHHHHHHH
PCLIREDFVCDEVDGKIYTYCSEGCRWTHKVAFAAEYEGRPTPAMGRFSGRREWEDCYHG
HHHHHCCCCCCCCCCCEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHCC
WDIADAIKDLGFVRPDGKTLTAQPHLRFDAKDMWTLDHVRGHQLGSPLRGFRALSPIERE
CCHHHHHHHCCCCCCCCCEEECCCCEEECCCHHEEHHHHCCCCCCCHHHHHHHCCCHHHH
IAVADYRKGFTINPCH
HHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SASLTLNKITAQKGITIAEAASRVADLGWTPSYVQEAMTFPTDYKISKTPRDPMKQVLR
CCCEEEHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCCCHHHHHHHHH
SYFPMQEEKDNRVYGALDAALRGDMFRNVEPRWVEWMKLFLAIIPFPEISAARSMAMVGR
HHCCCCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
LAPGEELRTGFTMQMVDEFRHSTIQMNLKKWYMENYIDPAGFDITEAAFGKCYATTIGRQ
CCCCHHHHCCCHHHHHHHHHHHHEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
FAEGFLTGDAITAANVYLTVVAETAFTNTLFVAMPSEAARNGDYALPTVFLSVQSDESRH
HHHHHCCCCCEECCEEEEEEEEHHHCCCEEEEEECCHHHCCCCEECEEEEEEEECCCCCC
IGNGHSMLMAMINDPSNHQLLERDLKYAFWQNHAIVDAAIGTFIEYGTTNRDKNKESYAE
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
LWHRWIYEDYYRTYMLPLEKYGIKIHHDDVAAAWDRIVKKNYVHKTAQFFAVGWSANFWR
HHHHHHHHHHHHHHEEEHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEE
IEAQTEKDFEWFEHKYPGWYAEFGDFWKWYAKLSVPGETNILFNSDVGYVYPHRCWSCMV
EEECCCCCHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCCEEEECCCCCCCHHHHHHHHH
PCLIREDFVCDEVDGKIYTYCSEGCRWTHKVAFAAEYEGRPTPAMGRFSGRREWEDCYHG
HHHHHCCCCCCCCCCCEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHCC
WDIADAIKDLGFVRPDGKTLTAQPHLRFDAKDMWTLDHVRGHQLGSPLRGFRALSPIERE
CCHHHHHHHCCCCCCCCCEEECCCCEEECCCHHEEHHHHCCCCCCCHHHHHHHCCCHHHH
IAVADYRKGFTINPCH
HHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2205538; 8255292; 9432288; 9329079; 11456616 [H]