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The map label for this gene is prpB [C]

Identifier: 146341246

GI number: 146341246

Start: 4516818

End: 4517633

Strand: Reverse

Name: prpB [C]

Synonym: BRADO4322

Alternate gene names: 146341246

Gene position: 4517633-4516818 (Counterclockwise)

Preceding gene: 146341247

Following gene: 146341243

Centisome position: 60.59

GC content: 66.18

Gene sequence:

>816_bases
ATGACGGTCACGACGGCGGACAAGCGCGCCGCATTCCGCAAGCTTCATGAGTCCGGCTGCTTCGTGCTGCCGAATCCGTT
CGATGCCGGCACGGCGAAGATGCTGCAGCATCTCGGCTTCAAGGCGATCGCTTCCACCAGCGCCGGCTTTGCCTGGTCGA
TCGGTAAGGCCGACACCCACACCATGCTGGACGAGATGCTCGCGCATCTGGCGGCGGTGTCGACGGCGGTCGACATTCCC
GTCAACGCCGATTTTGAGGGCGGCTTCGCCGAAGCGCCGGAGGGCGTGGCGGCCAATGTCGCGCGCGCGGTCGAGACCGG
CGTCGCGGGCCTGTCGATCGAGGATTCCACCGGTGACAAGGACCATCCGCTGTTCGCGCGCGATCTCGCAGTGGAGCGCA
TCCGCGCTGCGCGACAGGCGATCGATGCCAATGGCGGCGACGTGCTGCTGACCGGTCGATGCGAGGCGTTTCTCTGGGGC
AAGCCCGACCTCGGGCTCGTGACCGACCGTCTCGCGGCCTATGCCGAGGCCGGCGCGGATTGTCTGTATGCGCCAGGGAT
CAAGTCGGTCGAGGAGATCACGGCCGTCGTCAAAGCTGTTGCGCCGAAGCCGGTCAACCTGCTGATCGGGTCGTCAGGCC
TGTCGCTGATGCAGGCCGAAGATCTCGGTGTGCGACGCATCAGCGTCGGCGGCTCGCTGGCAAGGATAGCCTGGGCCGGC
TTCATGCGGGCCGCCAAGGAGATGGCCGAGCAGGGCACCTTCACCGAACTCGCCAACGGCTATCCCGGCGGTGAGTTGAA
CAAGATCTTCGGGTGA

Upstream 100 bases:

>100_bases
GCTCATTCTGGGCGAAGAGCGAACATTTCTGCGCAATGTCGTGGCCACGGCATGAGAGCGGTACTATGCTGGCGCAACCA
AGACGAGTTGGGAGGACGAC

Downstream 100 bases:

>100_bases
GATCGTTGTCTCGCACGAGGAAAAGCCGCGGCGTGGGCCGCGGCTTTTCTGTTTCCTGCAATCGTTGCGCTCAGTCGCGA
TCGACGCCAATGCCGACGCT

Product: putative methylisocitrate lyase or carboxyvinyl-carboxyphosphonate phosphorylmutase

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTVTTADKRAAFRKLHESGCFVLPNPFDAGTAKMLQHLGFKAIASTSAGFAWSIGKADTHTMLDEMLAHLAAVSTAVDIP
VNADFEGGFAEAPEGVAANVARAVETGVAGLSIEDSTGDKDHPLFARDLAVERIRAARQAIDANGGDVLLTGRCEAFLWG
KPDLGLVTDRLAAYAEAGADCLYAPGIKSVEEITAVVKAVAPKPVNLLIGSSGLSLMQAEDLGVRRISVGGSLARIAWAG
FMRAAKEMAEQGTFTELANGYPGGELNKIFG

Sequences:

>Translated_271_residues
MTVTTADKRAAFRKLHESGCFVLPNPFDAGTAKMLQHLGFKAIASTSAGFAWSIGKADTHTMLDEMLAHLAAVSTAVDIP
VNADFEGGFAEAPEGVAANVARAVETGVAGLSIEDSTGDKDHPLFARDLAVERIRAARQAIDANGGDVLLTGRCEAFLWG
KPDLGLVTDRLAAYAEAGADCLYAPGIKSVEEITAVVKAVAPKPVNLLIGSSGLSLMQAEDLGVRRISVGGSLARIAWAG
FMRAAKEMAEQGTFTELANGYPGGELNKIFG
>Mature_270_residues
TVTTADKRAAFRKLHESGCFVLPNPFDAGTAKMLQHLGFKAIASTSAGFAWSIGKADTHTMLDEMLAHLAAVSTAVDIPV
NADFEGGFAEAPEGVAANVARAVETGVAGLSIEDSTGDKDHPLFARDLAVERIRAARQAIDANGGDVLLTGRCEAFLWGK
PDLGLVTDRLAAYAEAGADCLYAPGIKSVEEITAVVKAVAPKPVNLLIGSSGLSLMQAEDLGVRRISVGGSLARIAWAGF
MRAAKEMAEQGTFTELANGYPGGELNKIFG

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 28276; Mature: 28145

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTTADKRAAFRKLHESGCFVLPNPFDAGTAKMLQHLGFKAIASTSAGFAWSIGKADTH
CCCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHCCHHHHHCCCCCCEEECCCCHHH
TMLDEMLAHLAAVSTAVDIPVNADFEGGFAEAPEGVAANVARAVETGVAGLSIEDSTGDK
HHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCC
DHPLFARDLAVERIRAARQAIDANGGDVLLTGRCEAFLWGKPDLGLVTDRLAAYAEAGAD
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCC
CLYAPGIKSVEEITAVVKAVAPKPVNLLIGSSGLSLMQAEDLGVRRISVGGSLARIAWAG
EEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCEEEEECCCHHHHHHHHH
FMRAAKEMAEQGTFTELANGYPGGELNKIFG
HHHHHHHHHHCCCHHHHHCCCCCCCHHHCCC
>Mature Secondary Structure 
TVTTADKRAAFRKLHESGCFVLPNPFDAGTAKMLQHLGFKAIASTSAGFAWSIGKADTH
CCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHCCHHHHHCCCCCCEEECCCCHHH
TMLDEMLAHLAAVSTAVDIPVNADFEGGFAEAPEGVAANVARAVETGVAGLSIEDSTGDK
HHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCC
DHPLFARDLAVERIRAARQAIDANGGDVLLTGRCEAFLWGKPDLGLVTDRLAAYAEAGAD
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCC
CLYAPGIKSVEEITAVVKAVAPKPVNLLIGSSGLSLMQAEDLGVRRISVGGSLARIAWAG
EEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCEEEEECCCHHHHHHHHH
FMRAAKEMAEQGTFTELANGYPGGELNKIFG
HHHHHHHHHHCCCHHHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]