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The map label for this gene is yhcK [H]

Identifier: 146341144

GI number: 146341144

Start: 4415959

End: 4417185

Strand: Reverse

Name: yhcK [H]

Synonym: BRADO4215

Alternate gene names: 146341144

Gene position: 4417185-4415959 (Counterclockwise)

Preceding gene: 146341145

Following gene: 146341143

Centisome position: 59.24

GC content: 68.13

Gene sequence:

>1227_bases
ATGGCACTGGACAGCCTGACGCTCGACATCGTAGCGACAATGGTCGCGGCGCTGCTTGGCGCCATGTTGCTGTACTTTGG
ACGGCAGGAGAACACCCCGGCGCTGCGCTGGTGGGGCGCGGCCTACATCCTCGGCGCGGTCGCGATCGCGCTATGGACGC
TGGTGGCCGACAGCTATGGCGAGGTCGTCTCGCTGTTCCTGAGTTCGATCGGACTGATCGCGACCGCCATGGTCTGGAAC
GCGGCGCGCGTCTTCCACGGCAAATCGCCGAGCGTGACGGGACTTGCGCTCGGCGCCATCGTGTGGATCGCCAGCGGCAT
GCTGCTCGATCCCGCGGCGCAGGCGCTGCGGCTCACCATCGGCGCCGCCATCGTCGTGATCTATGCGACCCTCACCGCGG
CGGAGCTGTGGCAGGAGCGCCGGCGCACCCTGCAGAAGCGCTGGCCCGCGGTTCTGGTGCCGGCCATGCACGGCTTCGTC
CTGATGCTGCCGATCCTGCTCGGCAACATGCTGCGCGATGCCAATGGCGCATTCGCCGCCGCCTGGGTGACGATCTTCTC
GATCGAACTGGTGCTCTATGCCGTCGGCACGGTGTTCGTCATCTTCATGCTGGTGTCCGAGCGCATCGTCTCCGCGCACA
AGCGCGCCGCGACCACCGACCCGCTCACCGGGCTGCTCAACCGGCGCGGCTTTGCGGATGCCTGCGCGCAACTGATCGAG
CGCGAGGCGCGCGCGCAGCAGCCGGTCAGCGTGCTGATCTTCGACATCGATCATTTCAAGTCGATCAACGACCGCTTCGG
TCATGCCGAGGGCGATGAGGTGCTGAAGATCTTCGCCGATGTTGTCGTGAATAATCTTCGCCTGACCGATCTGTCGGGGC
GGATCGGCGGCGAGGAGTTCGCGGCGCTCTTGCCCTGCTCGATCGAGGAGGCGATGGTCGCAGCCGAGCGTGTGCGCGAG
GCCTTCGCCGCGTCCGGCGTGGTCTGCGAGGAGAAACCCGTCGACACCACCGTGAGCATCGGCGTGGCCGGCGGGCCGGC
CGGAACCGAGCTCGACGTGCTGCTCGCCGCGGCCGACACCGCGCTCTACAAGGCCAAGCGCTCGGGCCGCAACCGCGTCG
AGGCCTCGGCGGAACTGCCGCTGTCGCTGGAGCGCTATCGCCGCCAGCGCGCGGCTGCCGCGACGGTCGCCGCATCACCG
TCCGGCCAGGCTGTCATCTCCGGCTGA

Upstream 100 bases:

>100_bases
CCCAGGCAAACCTCTTCCCAACAGACCACCGGTTAGGTTGATCGCGATAAGGCGGCGCTAACCCTTTGTTCCCGCACCGC
CGCAGCCACCGAGGTGACGC

Downstream 100 bases:

>100_bases
CGCTTCCGCTCCCCTGAGGTCTGGGCTACACACACGGTCCAACATCAGGGGAGGACGACCAGCATGATCACGGAAATCGC
CCAGATCGACGTCAAGCCCG

Product: putative diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MALDSLTLDIVATMVAALLGAMLLYFGRQENTPALRWWGAAYILGAVAIALWTLVADSYGEVVSLFLSSIGLIATAMVWN
AARVFHGKSPSVTGLALGAIVWIASGMLLDPAAQALRLTIGAAIVVIYATLTAAELWQERRRTLQKRWPAVLVPAMHGFV
LMLPILLGNMLRDANGAFAAAWVTIFSIELVLYAVGTVFVIFMLVSERIVSAHKRAATTDPLTGLLNRRGFADACAQLIE
REARAQQPVSVLIFDIDHFKSINDRFGHAEGDEVLKIFADVVVNNLRLTDLSGRIGGEEFAALLPCSIEEAMVAAERVRE
AFAASGVVCEEKPVDTTVSIGVAGGPAGTELDVLLAAADTALYKAKRSGRNRVEASAELPLSLERYRRQRAAAATVAASP
SGQAVISG

Sequences:

>Translated_408_residues
MALDSLTLDIVATMVAALLGAMLLYFGRQENTPALRWWGAAYILGAVAIALWTLVADSYGEVVSLFLSSIGLIATAMVWN
AARVFHGKSPSVTGLALGAIVWIASGMLLDPAAQALRLTIGAAIVVIYATLTAAELWQERRRTLQKRWPAVLVPAMHGFV
LMLPILLGNMLRDANGAFAAAWVTIFSIELVLYAVGTVFVIFMLVSERIVSAHKRAATTDPLTGLLNRRGFADACAQLIE
REARAQQPVSVLIFDIDHFKSINDRFGHAEGDEVLKIFADVVVNNLRLTDLSGRIGGEEFAALLPCSIEEAMVAAERVRE
AFAASGVVCEEKPVDTTVSIGVAGGPAGTELDVLLAAADTALYKAKRSGRNRVEASAELPLSLERYRRQRAAAATVAASP
SGQAVISG
>Mature_407_residues
ALDSLTLDIVATMVAALLGAMLLYFGRQENTPALRWWGAAYILGAVAIALWTLVADSYGEVVSLFLSSIGLIATAMVWNA
ARVFHGKSPSVTGLALGAIVWIASGMLLDPAAQALRLTIGAAIVVIYATLTAAELWQERRRTLQKRWPAVLVPAMHGFVL
MLPILLGNMLRDANGAFAAAWVTIFSIELVLYAVGTVFVIFMLVSERIVSAHKRAATTDPLTGLLNRRGFADACAQLIER
EARAQQPVSVLIFDIDHFKSINDRFGHAEGDEVLKIFADVVVNNLRLTDLSGRIGGEEFAALLPCSIEEAMVAAERVREA
FAASGVVCEEKPVDTTVSIGVAGGPAGTELDVLLAAADTALYKAKRSGRNRVEASAELPLSLERYRRQRAAAATVAASPS
GQAVISG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=199, Percent_Identity=38.6934673366834, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87082007, Length=184, Percent_Identity=39.1304347826087, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI145693134, Length=189, Percent_Identity=35.978835978836, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786584, Length=174, Percent_Identity=37.3563218390805, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI87081881, Length=194, Percent_Identity=36.5979381443299, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=36.4197530864198, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788381, Length=210, Percent_Identity=30.4761904761905, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=32.5, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788085, Length=179, Percent_Identity=31.2849162011173, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787541, Length=183, Percent_Identity=31.1475409836066, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081977, Length=156, Percent_Identity=31.4102564102564, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081974, Length=185, Percent_Identity=25.9459459459459, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788956, Length=156, Percent_Identity=32.6923076923077, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787056, Length=160, Percent_Identity=28.75, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620 [H]

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 43514; Mature: 43383

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDSLTLDIVATMVAALLGAMLLYFGRQENTPALRWWGAAYILGAVAIALWTLVADSYG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVVSLFLSSIGLIATAMVWNAARVFHGKSPSVTGLALGAIVWIASGMLLDPAAQALRLTI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GAAIVVIYATLTAAELWQERRRTLQKRWPAVLVPAMHGFVLMLPILLGNMLRDANGAFAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AWVTIFSIELVLYAVGTVFVIFMLVSERIVSAHKRAATTDPLTGLLNRRGFADACAQLIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHH
REARAQQPVSVLIFDIDHFKSINDRFGHAEGDEVLKIFADVVVNNLRLTDLSGRIGGEEF
HHHHHCCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHH
AALLPCSIEEAMVAAERVREAFAASGVVCEEKPVDTTVSIGVAGGPAGTELDVLLAAADT
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
ALYKAKRSGRNRVEASAELPLSLERYRRQRAAAATVAASPSGQAVISG
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECC
>Mature Secondary Structure 
ALDSLTLDIVATMVAALLGAMLLYFGRQENTPALRWWGAAYILGAVAIALWTLVADSYG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVVSLFLSSIGLIATAMVWNAARVFHGKSPSVTGLALGAIVWIASGMLLDPAAQALRLTI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GAAIVVIYATLTAAELWQERRRTLQKRWPAVLVPAMHGFVLMLPILLGNMLRDANGAFAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AWVTIFSIELVLYAVGTVFVIFMLVSERIVSAHKRAATTDPLTGLLNRRGFADACAQLIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHH
REARAQQPVSVLIFDIDHFKSINDRFGHAEGDEVLKIFADVVVNNLRLTDLSGRIGGEEF
HHHHHCCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHH
AALLPCSIEEAMVAAERVREAFAASGVVCEEKPVDTTVSIGVAGGPAGTELDVLLAAADT
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
ALYKAKRSGRNRVEASAELPLSLERYRRQRAAAATVAASPSGQAVISG
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]