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The map label for this gene is nuoB [H]

Identifier: 146341114

GI number: 146341114

Start: 4386911

End: 4387492

Strand: Reverse

Name: nuoB [H]

Synonym: BRADO4185

Alternate gene names: 146341114

Gene position: 4387492-4386911 (Counterclockwise)

Preceding gene: 146341115

Following gene: 146341113

Centisome position: 58.84

GC content: 65.29

Gene sequence:

>582_bases
TTGAGCCCGACTCCGTCCTCCGGTCCGGTGATCGCACCGGCCCCGAAGGGCATTCTCGATCCCGCGACCGGCAGGCCTGT
TGGTGCCCACGACCCGTATTTCCTCGAGGTCAAGCACGAGCTGTCGGACAAGGGATTCTTCGTTGCCACCGCTGACGACC
TCATCACCTGGGCCCGCACGGGCTCGCTGATGTGGATGACGTTCGGCCTGGCCTGCTGCGCCGTCGAGATGATGCAGGTC
TCGATGCCGCGCTACGACGTCGAGCGCTTCGGCTTCGCGCCGCGCGCCTCGCCGCGCCAGTCCGACGTGATGATCGTCGC
GGGCACCCTGACCAACAAGATGGCGCCGGCGCTGCGCAAGGTCTACGACCAGATGCCGGAGCCGCGCTACGTCATCTCGA
TGGGCTCCTGCGCCAATGGCGGCGGCTACTACCACTATTCCTATTCGGTGGTCCGCGGCTGCGATCGCATCGTTCCCATT
GACATCTATGTGCCCGGCTGTCCGCCGACCGCGGAAGCGCTGCTGTACGGTGTTCTGCTGCTGCAGAAGAAGATCCGTCG
CACCGGCACCATTGAACGCTGA

Upstream 100 bases:

>100_bases
GCAAGCTCGGCGCCGCCGGCTTCTGGTCGATGCTGGTGTTCCTGGCCGTGCTGACGGTCGGGTTTGCCTACGAATGGAAG
AAAGGCGCGCTTGAATGGGA

Downstream 100 bases:

>100_bases
AGGTTTTCGCTATGGACGACGGCAGGCTCGACGCCCTTGGGCAGACGATCGTGAGCGCGCTTCCGGGCGCGGCGCTCGGC
CACTCGGTCGCCTTCAATCA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MSPTPSSGPVIAPAPKGILDPATGRPVGAHDPYFLEVKHELSDKGFFVATADDLITWARTGSLMWMTFGLACCAVEMMQV
SMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPI
DIYVPGCPPTAEALLYGVLLLQKKIRRTGTIER

Sequences:

>Translated_193_residues
MSPTPSSGPVIAPAPKGILDPATGRPVGAHDPYFLEVKHELSDKGFFVATADDLITWARTGSLMWMTFGLACCAVEMMQV
SMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPI
DIYVPGCPPTAEALLYGVLLLQKKIRRTGTIER
>Mature_192_residues
SPTPSSGPVIAPAPKGILDPATGRPVGAHDPYFLEVKHELSDKGFFVATADDLITWARTGSLMWMTFGLACCAVEMMQVS
MPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPID
IYVPGCPPTAEALLYGVLLLQKKIRRTGTIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=152, Percent_Identity=77.6315789473684, Blast_Score=250, Evalue=6e-67,
Organism=Escherichia coli, GI1788624, Length=168, Percent_Identity=46.4285714285714, Blast_Score=159, Evalue=8e-41,
Organism=Escherichia coli, GI1789074, Length=126, Percent_Identity=42.8571428571429, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=42.6229508196721, Blast_Score=101, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17509685, Length=174, Percent_Identity=63.7931034482759, Blast_Score=226, Evalue=5e-60,
Organism=Drosophila melanogaster, GI18859983, Length=170, Percent_Identity=68.2352941176471, Blast_Score=248, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24642371, Length=170, Percent_Identity=68.2352941176471, Blast_Score=248, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24651058, Length=144, Percent_Identity=76.3888888888889, Blast_Score=242, Evalue=1e-64,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 21140; Mature: 21009

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTPSSGPVIAPAPKGILDPATGRPVGAHDPYFLEVKHELSDKGFFVATADDLITWART
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHC
GSLMWMTFGLACCAVEMMQVSMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRK
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHH
VYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPIDIYVPGCPPTAEALLYGVLL
HHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHH
LQKKIRRTGTIER
HHHHHHHCCCCCC
>Mature Secondary Structure 
SPTPSSGPVIAPAPKGILDPATGRPVGAHDPYFLEVKHELSDKGFFVATADDLITWART
CCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHC
GSLMWMTFGLACCAVEMMQVSMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRK
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHH
VYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPIDIYVPGCPPTAEALLYGVLL
HHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHH
LQKKIRRTGTIER
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]