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The map label for this gene is nuoE [H]

Identifier: 146341110

GI number: 146341110

Start: 4383498

End: 4384109

Strand: Reverse

Name: nuoE [H]

Synonym: BRADO4181

Alternate gene names: 146341110

Gene position: 4384109-4383498 (Counterclockwise)

Preceding gene: 146341111

Following gene: 146341109

Centisome position: 58.8

GC content: 65.03

Gene sequence:

>612_bases
ATGTCCGTCCGCCGTCTTGCCCCGAAGGAAGTCCAGCCCGCGAGCTTCGCGTTCACGGATGACAACCTTGCCTTCGCCAA
GGCTCAGATCGCCAAATACCCGGAAGGCCGCCAGGCGTCGGCGGTGATCGCGATCCTGTGGCGCGCGCAGGAGCAGAACG
AGGGCTGGGTCTCGGAAGCCGCGATCCGCGTCGTCGCCGACATGCTGGGCATGCCCTATATCCGCGTGCTGGAGATCGCG
ACTTTCTACACGATGTTCCAGCTGCAGCCGGTCGGCAAGAAGGCGCATGTCCAGGTCTGCGGAACCACGCCGTGCCGCCT
GCGCGGTGCCGAGGAACTGATCGAGGTCTGCAAGCACCGCATCCATCACGATCCGTTCCACCTCTCCAAGGATGGCGACT
TCAGCTGGGAAGAGGTGGAGTGCCTGGGCGCCTGTGTGAATGCGCCGATGGTGCAGGTGTGGAAGGATACCTATGAGGAC
CTGACGCCGGAAAGCTTCGGCAAGGTGCTCGACGGCTTCGCGACCGGCAACCTGCCGACGCCGGGTCCGCAGAACGGCCG
CCAGTTCTCGGCGCCGGCGGGCGGACCGACCACGCTGAAGGAGAAGACATGA

Upstream 100 bases:

>100_bases
GCTATAACGTGTTCGGAACTGCAAGTGCCTTCTGGCAGAATCGCGTCGCTGCGCTCAATGCAGGTGCCGCGCGCCGCATG
CAATGAAAGACTAGAGAGCC

Downstream 100 bases:

>100_bases
GCCGCCATCGCACCCGCGTTGCGGCCCTGAGCCAAGAGGGCGCCGCAATGAAGAACTGGCGCTACGTGGCCCTGCACACG
GTTGCGGCGGCCGCTTTCAT

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 1; NDH-1 subunit E 1 [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MSVRRLAPKEVQPASFAFTDDNLAFAKAQIAKYPEGRQASAVIAILWRAQEQNEGWVSEAAIRVVADMLGMPYIRVLEIA
TFYTMFQLQPVGKKAHVQVCGTTPCRLRGAEELIEVCKHRIHHDPFHLSKDGDFSWEEVECLGACVNAPMVQVWKDTYED
LTPESFGKVLDGFATGNLPTPGPQNGRQFSAPAGGPTTLKEKT

Sequences:

>Translated_203_residues
MSVRRLAPKEVQPASFAFTDDNLAFAKAQIAKYPEGRQASAVIAILWRAQEQNEGWVSEAAIRVVADMLGMPYIRVLEIA
TFYTMFQLQPVGKKAHVQVCGTTPCRLRGAEELIEVCKHRIHHDPFHLSKDGDFSWEEVECLGACVNAPMVQVWKDTYED
LTPESFGKVLDGFATGNLPTPGPQNGRQFSAPAGGPTTLKEKT
>Mature_202_residues
SVRRLAPKEVQPASFAFTDDNLAFAKAQIAKYPEGRQASAVIAILWRAQEQNEGWVSEAAIRVVADMLGMPYIRVLEIAT
FYTMFQLQPVGKKAHVQVCGTTPCRLRGAEELIEVCKHRIHHDPFHLSKDGDFSWEEVECLGACVNAPMVQVWKDTYEDL
TPESFGKVLDGFATGNLPTPGPQNGRQFSAPAGGPTTLKEKT

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=201, Percent_Identity=43.7810945273632, Blast_Score=171, Evalue=4e-43,
Organism=Escherichia coli, GI1788621, Length=162, Percent_Identity=29.6296296296296, Blast_Score=79, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17561328, Length=186, Percent_Identity=47.8494623655914, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI18859877, Length=200, Percent_Identity=44.5, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI21355353, Length=186, Percent_Identity=41.3978494623656, Blast_Score=146, Evalue=9e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 22434; Mature: 22303

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRRLAPKEVQPASFAFTDDNLAFAKAQIAKYPEGRQASAVIAILWRAQEQNEGWVSEA
CCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHEECCCCCCCCHHHHH
AIRVVADMLGMPYIRVLEIATFYTMFQLQPVGKKAHVQVCGTTPCRLRGAEELIEVCKHR
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHCCCHHHHHHHHHHH
IHHDPFHLSKDGDFSWEEVECLGACVNAPMVQVWKDTYEDLTPESFGKVLDGFATGNLPT
HCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
PGPQNGRQFSAPAGGPTTLKEKT
CCCCCCCEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SVRRLAPKEVQPASFAFTDDNLAFAKAQIAKYPEGRQASAVIAILWRAQEQNEGWVSEA
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHEECCCCCCCCHHHHH
AIRVVADMLGMPYIRVLEIATFYTMFQLQPVGKKAHVQVCGTTPCRLRGAEELIEVCKHR
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHCCCHHHHHHHHHHH
IHHDPFHLSKDGDFSWEEVECLGACVNAPMVQVWKDTYEDLTPESFGKVLDGFATGNLPT
HCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
PGPQNGRQFSAPAGGPTTLKEKT
CCCCCCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]