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The map label for this gene is lpxB [H]

Identifier: 146341056

GI number: 146341056

Start: 4322120

End: 4323316

Strand: Reverse

Name: lpxB [H]

Synonym: BRADO4127

Alternate gene names: 146341056

Gene position: 4323316-4322120 (Counterclockwise)

Preceding gene: 146341057

Following gene: 146341053

Centisome position: 57.98

GC content: 68.25

Gene sequence:

>1197_bases
GTGACGCCGCAGCCGTCCGCCAGCCCGGTCGTCCGCAGGATCTGCCTCGTCGCCACCGAAGAGTCGGGTGATCGCCTCGG
CGCCCCGCTGATGAAAGCGCTCCGCCAGCGCCTCGGTGACGGCGTCGTATTCTCCGGCGTCGGCGGCCGCGGCATGATCG
GCGAGGGTCTCGTCTCGCTGTTTCCGATCGAGGAGCTCTCGATCGTCGGCTTCACCGCGGTGATCAAGCAGCTGCCAAAG
ATTCTCGGACTGATCCGGCGCACCGTGGATGCGGTCGTGGCGGCGCAGCCCGACGTGCTCGTGATCATCGACAGCCCCGA
TTTCACCCAGCGCGTGGCGAAGCGCGTTCACGCGCGCGACGCGACGATCCCGATCGTCAACTACGCAGCGCCGACGGTGT
GGGCCTGGCGTCCCGGCCGCGCGCGCGTGATGCGCGGCTATCTCGATCACGTGCTCGGCCTGTTGCCCTTTGAGCCCGAC
GCGTATCGCAGGCTCGGTGGTCCGCCCTGCACCTATGTCGGCCATCCCCTCACGGAGCAGCTTGCGACGTTGCGGCCCGA
CACGGCCGAGCAGGCGCGCCGCGATGCCAAGCCGCCGGTTCTGCTGGTGCTGCCCGGCAGCCGCCGCAGCGAGGTCGCGC
GCCACATCGCGGTGTTCGGCGAGACGCTGGCGCGGCTGCAGGCGCAAGGCGTTGTGTTCGAGGCGGTGCTGCCGACCACG
CCGCATCTGGAAGCCGCGGTCAGGGCAGGGGTTGCGAGCTGGCCGGTGAAGCCCGCTATCATCATGGGGGACGCCGAGAA
GCGTGCCGCCTTTCGCAGTGCCCGCGCCGCGCTCGCCAAGTCCGGCACGGTGACGCTCGAGCTGGCGCTGTCGGGCGTGC
CGATGGTGACCGCCTATCGCGTCGGCGATGTCGAAGCCTTCATCCTGCGCCGTCTCATCAAGGTGCAGTCTGTCATTCTC
GCCAATCTCGTGATCGGCGACAACGTCATCCCGGAATTCCTGCAGGAAGCCTGCACGGCCGACAATCTCGCCCCCGTGCT
GGTCGATCTTTTGAACGACGGCCCGATCCGCATGCGGCAGGTCGAAGCCTTTAATGGTCTCGATGACATCATGGCCACCG
GTGCGGATTCACCAAGTGTCCGCGCGGCCGACATCGTGCTCGCGACAATGCGCGGCGGCCGACACGAACCGGCCTGA

Upstream 100 bases:

>100_bases
CAGGCGTCGCCGTGGCCTCTGCGTACACGCTGCTCGCCGATCCGCAGGAGACCGTGACCGCCGCCGACCGCGCCGGCGTG
TTCATCACGGGCGTGACGGC

Downstream 100 bases:

>100_bases
TCACACGGAGCGTAGGGTGGGCAAAGGCGCTGCCGTGCTCTTTGCCCCACGAACATTCGTTGCGGCGCCGGGCCCACCAT
CTCGCTGTTCGGCTCCGACG

Product: lipid-A-disaccharide synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTPQPSASPVVRRICLVATEESGDRLGAPLMKALRQRLGDGVVFSGVGGRGMIGEGLVSLFPIEELSIVGFTAVIKQLPK
ILGLIRRTVDAVVAAQPDVLVIIDSPDFTQRVAKRVHARDATIPIVNYAAPTVWAWRPGRARVMRGYLDHVLGLLPFEPD
AYRRLGGPPCTYVGHPLTEQLATLRPDTAEQARRDAKPPVLLVLPGSRRSEVARHIAVFGETLARLQAQGVVFEAVLPTT
PHLEAAVRAGVASWPVKPAIIMGDAEKRAAFRSARAALAKSGTVTLELALSGVPMVTAYRVGDVEAFILRRLIKVQSVIL
ANLVIGDNVIPEFLQEACTADNLAPVLVDLLNDGPIRMRQVEAFNGLDDIMATGADSPSVRAADIVLATMRGGRHEPA

Sequences:

>Translated_398_residues
MTPQPSASPVVRRICLVATEESGDRLGAPLMKALRQRLGDGVVFSGVGGRGMIGEGLVSLFPIEELSIVGFTAVIKQLPK
ILGLIRRTVDAVVAAQPDVLVIIDSPDFTQRVAKRVHARDATIPIVNYAAPTVWAWRPGRARVMRGYLDHVLGLLPFEPD
AYRRLGGPPCTYVGHPLTEQLATLRPDTAEQARRDAKPPVLLVLPGSRRSEVARHIAVFGETLARLQAQGVVFEAVLPTT
PHLEAAVRAGVASWPVKPAIIMGDAEKRAAFRSARAALAKSGTVTLELALSGVPMVTAYRVGDVEAFILRRLIKVQSVIL
ANLVIGDNVIPEFLQEACTADNLAPVLVDLLNDGPIRMRQVEAFNGLDDIMATGADSPSVRAADIVLATMRGGRHEPA
>Mature_397_residues
TPQPSASPVVRRICLVATEESGDRLGAPLMKALRQRLGDGVVFSGVGGRGMIGEGLVSLFPIEELSIVGFTAVIKQLPKI
LGLIRRTVDAVVAAQPDVLVIIDSPDFTQRVAKRVHARDATIPIVNYAAPTVWAWRPGRARVMRGYLDHVLGLLPFEPDA
YRRLGGPPCTYVGHPLTEQLATLRPDTAEQARRDAKPPVLLVLPGSRRSEVARHIAVFGETLARLQAQGVVFEAVLPTTP
HLEAAVRAGVASWPVKPAIIMGDAEKRAAFRSARAALAKSGTVTLELALSGVPMVTAYRVGDVEAFILRRLIKVQSVILA
NLVIGDNVIPEFLQEACTADNLAPVLVDLLNDGPIRMRQVEAFNGLDDIMATGADSPSVRAADIVLATMRGGRHEPA

Specific function: Condensation of UDP-2,3-diacylglucosamine and 2,3- diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell [H]

COG id: COG0763

COG function: function code M; Lipid A disaccharide synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lpxB family [H]

Homologues:

Organism=Escherichia coli, GI1786379, Length=376, Percent_Identity=32.7127659574468, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003835 [H]

Pfam domain/function: PF02684 LpxB [H]

EC number: =2.4.1.182 [H]

Molecular weight: Translated: 42676; Mature: 42545

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQPSASPVVRRICLVATEESGDRLGAPLMKALRQRLGDGVVFSGVGGRGMIGEGLVSL
CCCCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHH
FPIEELSIVGFTAVIKQLPKILGLIRRTVDAVVAAQPDVLVIIDSPDFTQRVAKRVHARD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCC
ATIPIVNYAAPTVWAWRPGRARVMRGYLDHVLGLLPFEPDAYRRLGGPPCTYVGHPLTEQ
CCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHH
LATLRPDTAEQARRDAKPPVLLVLPGSRRSEVARHIAVFGETLARLQAQGVVFEAVLPTT
HHHCCCCCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
PHLEAAVRAGVASWPVKPAIIMGDAEKRAAFRSARAALAKSGTVTLELALSGVPMVTAYR
CCHHHHHHCCHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEE
VGDVEAFILRRLIKVQSVILANLVIGDNVIPEFLQEACTADNLAPVLVDLLNDGPIRMRQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEH
VEAFNGLDDIMATGADSPSVRAADIVLATMRGGRHEPA
HHHHCCHHHHHHCCCCCCCCCHHHHHEEECCCCCCCCC
>Mature Secondary Structure 
TPQPSASPVVRRICLVATEESGDRLGAPLMKALRQRLGDGVVFSGVGGRGMIGEGLVSL
CCCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHH
FPIEELSIVGFTAVIKQLPKILGLIRRTVDAVVAAQPDVLVIIDSPDFTQRVAKRVHARD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCC
ATIPIVNYAAPTVWAWRPGRARVMRGYLDHVLGLLPFEPDAYRRLGGPPCTYVGHPLTEQ
CCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHH
LATLRPDTAEQARRDAKPPVLLVLPGSRRSEVARHIAVFGETLARLQAQGVVFEAVLPTT
HHHCCCCCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
PHLEAAVRAGVASWPVKPAIIMGDAEKRAAFRSARAALAKSGTVTLELALSGVPMVTAYR
CCHHHHHHCCHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEE
VGDVEAFILRRLIKVQSVILANLVIGDNVIPEFLQEACTADNLAPVLVDLLNDGPIRMRQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEH
VEAFNGLDDIMATGADSPSVRAADIVLATMRGGRHEPA
HHHHCCHHHHHHCCCCCCCCCHHHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]