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The map label for this gene is pyrG [H]

Identifier: 146341032

GI number: 146341032

Start: 4292696

End: 4294327

Strand: Reverse

Name: pyrG [H]

Synonym: BRADO4103

Alternate gene names: 146341032

Gene position: 4294327-4292696 (Counterclockwise)

Preceding gene: 146341033

Following gene: 146341031

Centisome position: 57.59

GC content: 65.38

Gene sequence:

>1632_bases
ATGGCGCGGTACATCTTCATCACCGGCGGCGTGGTTTCCTCGCTCGGAAAGGGTCTGGCTTCAGCCGCGTTAGGCGCGCT
GCTGCAGGCCCGCGGCTACAAGGTCCGGCTTCGCAAGCTCGACCCCTATCTCAACCTCGATCCAGGCACGATGTCGCCGT
ATCAGCACGGCGAAGTGTTCGTGACCGATGACGGCGCGGAGACCGATCTCGATCTCGGCCATTACGAACGCTTCACCGGG
CGGCCCGCGACCAAGGCCGACAACATCACCACGGGGCGCATCTACCAGGACATCCTGACCAAGGAGCGCCGCGGCGATTA
TCTCGGAGCGACCATCCAGGTCGTCCCGCATGTGACCAATGCGATCAAGGATTTCGTGCTGTCGGGCAATGACGAGTACG
ACTTCGTGCTGGTCGAGATCGGCGGCACCGTCGGCGACATCGAGGGCCTGCCGTTCTTCGAGGCGATCCGCCAACTCGGC
AACGAGCTGCCGCGCCACCACGCGATCTACATTCATCTCACGCTGCTGCCGTACATCCCGAGCGCCGGCGAATTGAAGAC
CAAGCCGACACAGCATTCGGTCAAGGAGCTGCGCTCGATCGGCATCCAGCCGGACATCCTGCTGTGCCGCACCGATCGCG
AGATCCCGAAGGAGGAGCGGCGCAAGCTCGGCCTGTTCTGCAACGTGCGCGAGAGCGCCGTGATCGAGGCGCGCGACGTC
GACAACATCTACGCCGTGCCGGAGGCCTATCACGCCGCCGGCCTCGACGACGAGGTGCTGGCCGCGTTCGGGATCGAGCC
GCGGATTCCGCCGGCGCTTGCGAGCTGGCACACGATCAACGAGCGCGTCCGTAATCCGGAAGGCGACGTGACGATCGCGA
TCGTCGGCAAGTACACGGGCATGAAGGATGCCTACAAGTCGCTGATCGAAGCGCTGTCCCATGGCGGCATCGCCAACAAG
GTCCGCGTCAAGCTCGACTGGATCGAGAGCGAGGTGTTCGAGAACGAGGACCCCGCGCCGTTCCTGGAGCACGTCAACGG
CATCCTGGTGCCCGGCGGCTTCGGCCAGCGCGGCGCGGAAGGCAAGATCCGCGCGGCGCAGTTCGCCAGGGAGCGCGACG
TGCCGTATTTCGGCATCTGTTTCGGCATGCAGATGGCGGTGATCGAAGCAGCGCGCAATCTCGTCGGAATCACCGAGGCA
AACTCGACCGAGTTCGGCCCGACCAAGGAGCCGCTGGTCGGTCTGATGACCGAGTGGCTGCGCGGCAACGAACTGGAGAA
GCGCTCGCAGAGCGGCGATCTCGGCGGCACGATGCGGCTCGGCGCCTATCCCGCGACGCTCAAGCGCGGCAGCCGCGTGT
CCGAGGTCTATGGCGGCGCCACCGAGATCTCGGAGCGGCACCGCCATCGCTACGAGGTCAACACCGCCTACAAGGACCGG
CTCGAGCAGCATGGCTTGAAGTTCTCCGGCCTGTCGCCCGACGGCGTGCTGCCGGAGATCGTGGAGTACGAGGACCATCC
CTGGTTCATCGGCGTCCAGTTCCACCCCGAGCTGAAGTCGCGCCCGTTCGAGCCGCACCCGCTGTTCGCATCGTTCATCC
AGGCGGCGATGGTGCAGAGCCGGCTGGTGTGA

Upstream 100 bases:

>100_bases
CATTCATGTTTTGACGTAACCCACAGGCCCCCGACATCGTTGCGGGCTGGTCACGATTCGTTTTGGCGAATCGAGCGCGC
GGCATTAAAGGTAGGGTCCC

Downstream 100 bases:

>100_bases
TCGGTTGCTGCCCCCGGGCAAGCCGTCATTGCGAGCGAAGCGAAGCAATCCAGAGTGCTTGCGCAGCCCTGGATTGCTTC
GTCGCTGACGCTCCTCGCAA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNLDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTG
RPATKADNITTGRIYQDILTKERRGDYLGATIQVVPHVTNAIKDFVLSGNDEYDFVLVEIGGTVGDIEGLPFFEAIRQLG
NELPRHHAIYIHLTLLPYIPSAGELKTKPTQHSVKELRSIGIQPDILLCRTDREIPKEERRKLGLFCNVRESAVIEARDV
DNIYAVPEAYHAAGLDDEVLAAFGIEPRIPPALASWHTINERVRNPEGDVTIAIVGKYTGMKDAYKSLIEALSHGGIANK
VRVKLDWIESEVFENEDPAPFLEHVNGILVPGGFGQRGAEGKIRAAQFARERDVPYFGICFGMQMAVIEAARNLVGITEA
NSTEFGPTKEPLVGLMTEWLRGNELEKRSQSGDLGGTMRLGAYPATLKRGSRVSEVYGGATEISERHRHRYEVNTAYKDR
LEQHGLKFSGLSPDGVLPEIVEYEDHPWFIGVQFHPELKSRPFEPHPLFASFIQAAMVQSRLV

Sequences:

>Translated_543_residues
MARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNLDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTG
RPATKADNITTGRIYQDILTKERRGDYLGATIQVVPHVTNAIKDFVLSGNDEYDFVLVEIGGTVGDIEGLPFFEAIRQLG
NELPRHHAIYIHLTLLPYIPSAGELKTKPTQHSVKELRSIGIQPDILLCRTDREIPKEERRKLGLFCNVRESAVIEARDV
DNIYAVPEAYHAAGLDDEVLAAFGIEPRIPPALASWHTINERVRNPEGDVTIAIVGKYTGMKDAYKSLIEALSHGGIANK
VRVKLDWIESEVFENEDPAPFLEHVNGILVPGGFGQRGAEGKIRAAQFARERDVPYFGICFGMQMAVIEAARNLVGITEA
NSTEFGPTKEPLVGLMTEWLRGNELEKRSQSGDLGGTMRLGAYPATLKRGSRVSEVYGGATEISERHRHRYEVNTAYKDR
LEQHGLKFSGLSPDGVLPEIVEYEDHPWFIGVQFHPELKSRPFEPHPLFASFIQAAMVQSRLV
>Mature_542_residues
ARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNLDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGR
PATKADNITTGRIYQDILTKERRGDYLGATIQVVPHVTNAIKDFVLSGNDEYDFVLVEIGGTVGDIEGLPFFEAIRQLGN
ELPRHHAIYIHLTLLPYIPSAGELKTKPTQHSVKELRSIGIQPDILLCRTDREIPKEERRKLGLFCNVRESAVIEARDVD
NIYAVPEAYHAAGLDDEVLAAFGIEPRIPPALASWHTINERVRNPEGDVTIAIVGKYTGMKDAYKSLIEALSHGGIANKV
RVKLDWIESEVFENEDPAPFLEHVNGILVPGGFGQRGAEGKIRAAQFARERDVPYFGICFGMQMAVIEAARNLVGITEAN
STEFGPTKEPLVGLMTEWLRGNELEKRSQSGDLGGTMRLGAYPATLKRGSRVSEVYGGATEISERHRHRYEVNTAYKDRL
EQHGLKFSGLSPDGVLPEIVEYEDHPWFIGVQFHPELKSRPFEPHPLFASFIQAAMVQSRLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=557, Percent_Identity=43.8061041292639, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI28559085, Length=560, Percent_Identity=42.8571428571429, Blast_Score=460, Evalue=1e-129,
Organism=Homo sapiens, GI28559083, Length=560, Percent_Identity=42.8571428571429, Blast_Score=460, Evalue=1e-129,
Organism=Homo sapiens, GI221316689, Length=560, Percent_Identity=42.8571428571429, Blast_Score=460, Evalue=1e-129,
Organism=Escherichia coli, GI1789142, Length=536, Percent_Identity=57.4626865671642, Blast_Score=604, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI25148299, Length=612, Percent_Identity=36.7647058823529, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6322563, Length=570, Percent_Identity=40.3508771929825, Blast_Score=431, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6319432, Length=567, Percent_Identity=40.3880070546737, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24664469, Length=558, Percent_Identity=43.5483870967742, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI21357815, Length=504, Percent_Identity=42.6587301587302, Blast_Score=395, Evalue=1e-110,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60228; Mature: 60097

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNLDPGTMSPYQHGEVF
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGRPATKADNITTGRIYQDILTKERRGDYLGATIQVVPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
AIKDFVLSGNDEYDFVLVEIGGTVGDIEGLPFFEAIRQLGNELPRHHAIYIHLTLLPYIP
HHHHHHHCCCCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCC
SAGELKTKPTQHSVKELRSIGIQPDILLCRTDREIPKEERRKLGLFCNVRESAVIEARDV
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHCCEEEECCCCCEEEECCC
DNIYAVPEAYHAAGLDDEVLAAFGIEPRIPPALASWHTINERVRNPEGDVTIAIVGKYTG
CCEEECCCHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
MKDAYKSLIEALSHGGIANKVRVKLDWIESEVFENEDPAPFLEHVNGILVPGGFGQRGAE
HHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHCCCCCCHHHHHCCCEEECCCCCCCCCC
GKIRAAQFARERDVPYFGICFGMQMAVIEAARNLVGITEANSTEFGPTKEPLVGLMTEWL
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHH
RGNELEKRSQSGDLGGTMRLGAYPATLKRGSRVSEVYGGATEISERHRHRYEVNTAYKDR
CCCHHHHHHCCCCCCCEEEECCCHHHHHCCCHHHHHHCCHHHHHHHHHHHEECCHHHHHH
LEQHGLKFSGLSPDGVLPEIVEYEDHPWFIGVQFHPELKSRPFEPHPLFASFIQAAMVQS
HHHCCCEECCCCCCCCCCHHHCCCCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHH
RLV
HCC
>Mature Secondary Structure 
ARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNLDPGTMSPYQHGEVF
CEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGRPATKADNITTGRIYQDILTKERRGDYLGATIQVVPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
AIKDFVLSGNDEYDFVLVEIGGTVGDIEGLPFFEAIRQLGNELPRHHAIYIHLTLLPYIP
HHHHHHHCCCCCCCEEEEEECCCEECCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCC
SAGELKTKPTQHSVKELRSIGIQPDILLCRTDREIPKEERRKLGLFCNVRESAVIEARDV
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHCCEEEECCCCCEEEECCC
DNIYAVPEAYHAAGLDDEVLAAFGIEPRIPPALASWHTINERVRNPEGDVTIAIVGKYTG
CCEEECCCHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
MKDAYKSLIEALSHGGIANKVRVKLDWIESEVFENEDPAPFLEHVNGILVPGGFGQRGAE
HHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHCCCCCCHHHHHCCCEEECCCCCCCCCC
GKIRAAQFARERDVPYFGICFGMQMAVIEAARNLVGITEANSTEFGPTKEPLVGLMTEWL
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHH
RGNELEKRSQSGDLGGTMRLGAYPATLKRGSRVSEVYGGATEISERHRHRYEVNTAYKDR
CCCHHHHHHCCCCCCCEEEECCCHHHHHCCCHHHHHHCCHHHHHHHHHHHEECCHHHHHH
LEQHGLKFSGLSPDGVLPEIVEYEDHPWFIGVQFHPELKSRPFEPHPLFASFIQAAMVQS
HHHCCCEECCCCCCCCCCHHHCCCCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHH
RLV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]