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The map label for this gene is nylB [H]

Identifier: 146341020

GI number: 146341020

Start: 4280468

End: 4281637

Strand: Reverse

Name: nylB [H]

Synonym: BRADO4091

Alternate gene names: 146341020

Gene position: 4281637-4280468 (Counterclockwise)

Preceding gene: 146341021

Following gene: 146341019

Centisome position: 57.42

GC content: 69.06

Gene sequence:

>1170_bases
ATGTCGACCGATCCGTCTCCCTCCAGCGCCGATCTCTCGAACTGGCGCAGCGCGCCGTTCAGCCGCTGGGCCTTTCACAA
CATCCGGTCCATCCTGCCGGTGGCCGGCGTCGCGAGCGCGCCGGGCAGCGCCTGGCCGCTGCCGGCGAGCCCGGTCTCGT
TCGCGGATTTCAGCCTGAAGCTGCCGAAGGGCGGCACGCTCGATCTCGATGGTTTCCTGAGAGCGACCGCGACCGATGGG
CTGCTGATCCTGCACGACGGCCGCATCGTGTTCGAGGCTTACGACGGCGGCACCGATCGCGACACGCCGCACATCCTGAT
GTCGGCGACCAAATCGATCACCGGCCTGATGCTCGGCATTCTCGCCGGTCGCGGCGAGATCAATCTCGACGCCGAGGTCA
CGCGCTACGTGCCGGAGGTAGCCGAGACCGCGTATCAGGGCGCCACGATCCGGCAGCTGACGGACATGCGCGCCGGCATC
GTGTTCGACGCCGCGGGGCTGCAGGCCTACGCCGACGCCGCGGGCTGGGAGCCGGTGGCACCGGGCACGACGCCGAACCT
GCACGACTTCTTCGCGACGATGCGCGCGCAGGCCAGGCCGCATGGCGGGCCGTTCAGCTACGTCTCGTCCAACACCGATC
TGATCGGCTGGGCGATCGAGCGCGCCACCGGCCGCAGCTTTGCGAGCCTGGTCGGCACCCTGCTGTGGCAGCCGATGGGC
GCGAGCGGCGAGGCCTTCATCACGCTCGACCGCAAGGGCGCGCCGCGCTGCACCGGCGGCTTCTGCGCGACGTTGCGCGA
TTTCGCCCGGCTGGGCCAGCTCGTGCTGTCGGGCGGACGCCGCGGCTCGCATGCGATCATTCCGCAAGCCTGGCTCGACG
ACATCAGGCAGAACGGCGACGCCGAGGCGTGGCGCGACGGCCAATGGCGCGACAGCTTTGCAGCCATCAGCCGCAACATG
CACTACCGCGGCGGATGGTACGTCATCAACGACCAGCCGCAGCTGATGTTCGCGATGGGCATCCACGGCCAGAACCTGTT
CATCGATGCCGCCAACGCGATCGTGATCGCCAAGGTCTCATCCTGGCCGCAGCCGGTCGACGGCCAGGCGATCTGGCTGA
CGCATCAGGCGGTGGCCGAATTCACCCGCTGCATCAGGGCGGCGCGCTGA

Upstream 100 bases:

>100_bases
CTCCGGGCGGCCTGTTCGTTTTTGCATACGCGAAATGCCGGCCTTTCCCCCGCTGCCGTGAACGCCTATGTCGGACGCCT
CGCAATCCGCAGGGCTCCGC

Downstream 100 bases:

>100_bases
TCTCCGCGCGCGCGGCGAGGCGCGGCAACGTGCCGGCCTGTCCCGCCGTGAAACGCTGAGTTTCGCATCCCGCTATTTTC
ATGCACTTGCATGTTTCCGG

Product: putative beta-lactamase family protein 6-aminohexanoate-dimer hydrolase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSTDPSPSSADLSNWRSAPFSRWAFHNIRSILPVAGVASAPGSAWPLPASPVSFADFSLKLPKGGTLDLDGFLRATATDG
LLILHDGRIVFEAYDGGTDRDTPHILMSATKSITGLMLGILAGRGEINLDAEVTRYVPEVAETAYQGATIRQLTDMRAGI
VFDAAGLQAYADAAGWEPVAPGTTPNLHDFFATMRAQARPHGGPFSYVSSNTDLIGWAIERATGRSFASLVGTLLWQPMG
ASGEAFITLDRKGAPRCTGGFCATLRDFARLGQLVLSGGRRGSHAIIPQAWLDDIRQNGDAEAWRDGQWRDSFAAISRNM
HYRGGWYVINDQPQLMFAMGIHGQNLFIDAANAIVIAKVSSWPQPVDGQAIWLTHQAVAEFTRCIRAAR

Sequences:

>Translated_389_residues
MSTDPSPSSADLSNWRSAPFSRWAFHNIRSILPVAGVASAPGSAWPLPASPVSFADFSLKLPKGGTLDLDGFLRATATDG
LLILHDGRIVFEAYDGGTDRDTPHILMSATKSITGLMLGILAGRGEINLDAEVTRYVPEVAETAYQGATIRQLTDMRAGI
VFDAAGLQAYADAAGWEPVAPGTTPNLHDFFATMRAQARPHGGPFSYVSSNTDLIGWAIERATGRSFASLVGTLLWQPMG
ASGEAFITLDRKGAPRCTGGFCATLRDFARLGQLVLSGGRRGSHAIIPQAWLDDIRQNGDAEAWRDGQWRDSFAAISRNM
HYRGGWYVINDQPQLMFAMGIHGQNLFIDAANAIVIAKVSSWPQPVDGQAIWLTHQAVAEFTRCIRAAR
>Mature_388_residues
STDPSPSSADLSNWRSAPFSRWAFHNIRSILPVAGVASAPGSAWPLPASPVSFADFSLKLPKGGTLDLDGFLRATATDGL
LILHDGRIVFEAYDGGTDRDTPHILMSATKSITGLMLGILAGRGEINLDAEVTRYVPEVAETAYQGATIRQLTDMRAGIV
FDAAGLQAYADAAGWEPVAPGTTPNLHDFFATMRAQARPHGGPFSYVSSNTDLIGWAIERATGRSFASLVGTLLWQPMGA
SGEAFITLDRKGAPRCTGGFCATLRDFARLGQLVLSGGRRGSHAIIPQAWLDDIRQNGDAEAWRDGQWRDSFAAISRNMH
YRGGWYVINDQPQLMFAMGIHGQNLFIDAANAIVIAKVSSWPQPVDGQAIWLTHQAVAEFTRCIRAAR

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 41957; Mature: 41826

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDPSPSSADLSNWRSAPFSRWAFHNIRSILPVAGVASAPGSAWPLPASPVSFADFSLK
CCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEE
LPKGGTLDLDGFLRATATDGLLILHDGRIVFEAYDGGTDRDTPHILMSATKSITGLMLGI
CCCCCCCCHHHHEEEECCCCEEEEECCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
LAGRGEINLDAEVTRYVPEVAETAYQGATIRQLTDMRAGIVFDAAGLQAYADAAGWEPVA
HCCCCCEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEECCCCHHHHHCCCCCCCC
PGTTPNLHDFFATMRAQARPHGGPFSYVSSNTDLIGWAIERATGRSFASLVGTLLWQPMG
CCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEEEEHHCCHHHHHHHHHHHHCCCC
ASGEAFITLDRKGAPRCTGGFCATLRDFARLGQLVLSGGRRGSHAIIPQAWLDDIRQNGD
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC
AEAWRDGQWRDSFAAISRNMHYRGGWYVINDQPQLMFAMGIHGQNLFIDAANAIVIAKVS
CCCCCCCCCHHHHHHHHCCCEECCCEEEECCCCCEEEEEECCCCEEEEEECCEEEEEEEC
SWPQPVDGQAIWLTHQAVAEFTRCIRAAR
CCCCCCCCCEEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STDPSPSSADLSNWRSAPFSRWAFHNIRSILPVAGVASAPGSAWPLPASPVSFADFSLK
CCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEE
LPKGGTLDLDGFLRATATDGLLILHDGRIVFEAYDGGTDRDTPHILMSATKSITGLMLGI
CCCCCCCCHHHHEEEECCCCEEEEECCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
LAGRGEINLDAEVTRYVPEVAETAYQGATIRQLTDMRAGIVFDAAGLQAYADAAGWEPVA
HCCCCCEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEECCCCHHHHHCCCCCCCC
PGTTPNLHDFFATMRAQARPHGGPFSYVSSNTDLIGWAIERATGRSFASLVGTLLWQPMG
CCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEEEEHHCCHHHHHHHHHHHHCCCC
ASGEAFITLDRKGAPRCTGGFCATLRDFARLGQLVLSGGRRGSHAIIPQAWLDDIRQNGD
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC
AEAWRDGQWRDSFAAISRNMHYRGGWYVINDQPQLMFAMGIHGQNLFIDAANAIVIAKVS
CCCCCCCCCHHHHHHHHCCCEECCCEEEECCCCCEEEEEECCCCEEEEEECCEEEEEEEC
SWPQPVDGQAIWLTHQAVAEFTRCIRAAR
CCCCCCCCCEEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 2512123 [H]