Click here to switch to the map view.

The map label for this gene is 146341000

Identifier: 146341000

GI number: 146341000

Start: 4259205

End: 4260719

Strand: Reverse

Name: 146341000

Synonym: BRADO4071

Alternate gene names: NA

Gene position: 4260719-4259205 (Counterclockwise)

Preceding gene: 146341001

Following gene: 146340999

Centisome position: 57.14

GC content: 66.14

Gene sequence:

>1515_bases
ATGTCAATCAAACGCCCGAATGCCGAGGAAGTCGCCGATCTCGCCGCCAGCCTGCACATGAATATGACGGTCGAGGAGGC
CGCCGAATATCTGTCGCTGATGGGCGGCATGTTCGATCAATACGACATCATCGATGAACTGCCGAACCCGCTGCCGCCGG
TGAAATATCCGCGCACGCCCGGCTATAAGCCGATGCCCAAGGAGAACAAGTACAACGCCTGGGCGATCAAGACCGAGGTC
AAGGGCGCGTCCTCCGGCAAGCTCGCCGGCCGCACCGTCGTGCTGAAGGACAACGTCGCGCTCGCCGGCGTTCCGATGAT
GAACGGCTCGACCACGCTCGAAGGCTTCATCCCCGCCGCGGACGCGACCATCGTCACCCGCATCCTCGATGCCGGCGGTA
CCATCGTCGGCAAGGCCGTGTGCGAGCACTTCTGCCTCTCCGGCGGCAGCCACACCTCCGATCCGGCACCGGTGCACAAT
CCCCTGAAGATGGGCTACTCGGCCGGCGGCTCGTCCTCGGGCAGCGCCGCCCTCGTGGCGGCCGGCGAGGTCGATATGTC
CATCGGCGGCGACCAGGGCGGCTCGATCCGCATCCCCGCGTCCTATTGCGGCATCTACGGCATGAAGGCGACGCACGGCC
TCGTGCCCTACACCGGTGTGATGCCGATCGAGTCGACGATCGACCACACCGGCCCGATGACGGCCAACGTCAAGGACAAC
GCGCTGCTGCTCGAAGTGCTGGCCGGCGCCGACGGCCTCGATCCGCGGCAATACGCGCCGAAGGTCGCGGCCTACACCGA
GTCGCTCGGCAAGGGCGTCAAGGGCCTGAAGATCGGTGTGCTCAAGGAAGGCTTCAGCGCGCCGAACATGGAACAGGGCG
TGGTCAGCAAGGTCAAGGCCGGCGCCGAGCGCTTCGCCAAGATGGGCGCCTCGGTGTCGGAAGTCTCGATCCCCGAGCAC
ATGCATGCGCTCGCGGCGTGGAATCCGATCACGCTGGAAGGCTTCCTGGTGCAGATGATGCTCGGCAACGGCATGGGCTT
CAACTGGAAGGGCCTCTATGACGTCGGTCTGCTCGACGCGCATTCCGGCTGGCGCACCCGTGCGGACGATCTCTCGGTGA
CGTTGAAGCTGACCATGCTGGTCGGCCAGTGGGGCCTCGAGCATTATCGCGGCCGCTACTACGCCAAGTCGCGCAACATC
GCGATCGCCGCCAAGGCGGCCTATGACGCGGTGTTCGGCTCCTACGACCTCTTGCTGATGCCGACCTTGCCCTGCGTCGC
CACACCGATCCCGGCCAAGGACGCGCCGCTCGCCGAAGTCGTGCAGCGCGCCTTCGAGATGACCGCGACGACGAGCCCGT
TCGACGTCACCGGTCATCCGGCGATGACCCTGCCGTGCGGCCTCTCCGACGGCCTGCCGGTCGGCCTGATGCTGATCGGC
AAGGACTACGCCGAGGCCACGATCTACCAGGCGGCGGCGGCGTTCGAGGCCGACGGCGACTGGAAGACCTTCTGA

Upstream 100 bases:

>100_bases
GGTCCCGAGCTAGACGAATTCCGGCGCGGCTGCGCCGGCGCAACTGAACTGCACACTCTTAGTCTCTGCTCCAGCTTGTC
CTCACAAGAAAGGATGACTC

Downstream 100 bases:

>100_bases
TGATCACCATCACCGCGGTCATCAGGGCCAAGGCGGGACACGAGGCCACGATGCGCGACGCTCTCGTCGCCGTCGCGGCC
CATGTCGCGGCCAACGAGCC

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MSIKRPNAEEVADLAASLHMNMTVEEAAEYLSLMGGMFDQYDIIDELPNPLPPVKYPRTPGYKPMPKENKYNAWAIKTEV
KGASSGKLAGRTVVLKDNVALAGVPMMNGSTTLEGFIPAADATIVTRILDAGGTIVGKAVCEHFCLSGGSHTSDPAPVHN
PLKMGYSAGGSSSGSAALVAAGEVDMSIGGDQGGSIRIPASYCGIYGMKATHGLVPYTGVMPIESTIDHTGPMTANVKDN
ALLLEVLAGADGLDPRQYAPKVAAYTESLGKGVKGLKIGVLKEGFSAPNMEQGVVSKVKAGAERFAKMGASVSEVSIPEH
MHALAAWNPITLEGFLVQMMLGNGMGFNWKGLYDVGLLDAHSGWRTRADDLSVTLKLTMLVGQWGLEHYRGRYYAKSRNI
AIAAKAAYDAVFGSYDLLLMPTLPCVATPIPAKDAPLAEVVQRAFEMTATTSPFDVTGHPAMTLPCGLSDGLPVGLMLIG
KDYAEATIYQAAAAFEADGDWKTF

Sequences:

>Translated_504_residues
MSIKRPNAEEVADLAASLHMNMTVEEAAEYLSLMGGMFDQYDIIDELPNPLPPVKYPRTPGYKPMPKENKYNAWAIKTEV
KGASSGKLAGRTVVLKDNVALAGVPMMNGSTTLEGFIPAADATIVTRILDAGGTIVGKAVCEHFCLSGGSHTSDPAPVHN
PLKMGYSAGGSSSGSAALVAAGEVDMSIGGDQGGSIRIPASYCGIYGMKATHGLVPYTGVMPIESTIDHTGPMTANVKDN
ALLLEVLAGADGLDPRQYAPKVAAYTESLGKGVKGLKIGVLKEGFSAPNMEQGVVSKVKAGAERFAKMGASVSEVSIPEH
MHALAAWNPITLEGFLVQMMLGNGMGFNWKGLYDVGLLDAHSGWRTRADDLSVTLKLTMLVGQWGLEHYRGRYYAKSRNI
AIAAKAAYDAVFGSYDLLLMPTLPCVATPIPAKDAPLAEVVQRAFEMTATTSPFDVTGHPAMTLPCGLSDGLPVGLMLIG
KDYAEATIYQAAAAFEADGDWKTF
>Mature_503_residues
SIKRPNAEEVADLAASLHMNMTVEEAAEYLSLMGGMFDQYDIIDELPNPLPPVKYPRTPGYKPMPKENKYNAWAIKTEVK
GASSGKLAGRTVVLKDNVALAGVPMMNGSTTLEGFIPAADATIVTRILDAGGTIVGKAVCEHFCLSGGSHTSDPAPVHNP
LKMGYSAGGSSSGSAALVAAGEVDMSIGGDQGGSIRIPASYCGIYGMKATHGLVPYTGVMPIESTIDHTGPMTANVKDNA
LLLEVLAGADGLDPRQYAPKVAAYTESLGKGVKGLKIGVLKEGFSAPNMEQGVVSKVKAGAERFAKMGASVSEVSIPEHM
HALAAWNPITLEGFLVQMMLGNGMGFNWKGLYDVGLLDAHSGWRTRADDLSVTLKLTMLVGQWGLEHYRGRYYAKSRNIA
IAAKAAYDAVFGSYDLLLMPTLPCVATPIPAKDAPLAEVVQRAFEMTATTSPFDVTGHPAMTLPCGLSDGLPVGLMLIGK
DYAEATIYQAAAAFEADGDWKTF

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=440, Percent_Identity=27.2727272727273, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI195972892, Length=430, Percent_Identity=25.8139534883721, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=424, Percent_Identity=24.5283018867925, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17537465, Length=395, Percent_Identity=23.5443037974684, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=426, Percent_Identity=24.8826291079812, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6319685, Length=436, Percent_Identity=23.394495412844, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648113, Length=445, Percent_Identity=28.7640449438202, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24644968, Length=436, Percent_Identity=23.394495412844, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI161078093, Length=433, Percent_Identity=25.635103926097, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45550774, Length=170, Percent_Identity=31.1764705882353, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648435, Length=170, Percent_Identity=31.1764705882353, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648437, Length=170, Percent_Identity=31.1764705882353, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648441, Length=170, Percent_Identity=31.1764705882353, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648439, Length=170, Percent_Identity=31.1764705882353, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356731, Length=156, Percent_Identity=32.6923076923077, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 53170; Mature: 53038

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKRPNAEEVADLAASLHMNMTVEEAAEYLSLMGGMFDQYDIIDELPNPLPPVKYPRTP
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCC
GYKPMPKENKYNAWAIKTEVKGASSGKLAGRTVVLKDNVALAGVPMMNGSTTLEGFIPAA
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCEEEEEEEECCCCCCEEEEECCC
DATIVTRILDAGGTIVGKAVCEHFCLSGGSHTSDPAPVHNPLKMGYSAGGSSSGSAALVA
CHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEEEE
AGEVDMSIGGDQGGSIRIPASYCGIYGMKATHGLVPYTGVMPIESTIDHTGPMTANVKDN
ECCEEEEECCCCCCEEEECHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEECCCCCC
ALLLEVLAGADGLDPRQYAPKVAAYTESLGKGVKGLKIGVLKEGFSAPNMEQGVVSKVKA
CEEEHHHHCCCCCCHHHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHH
GAERFAKMGASVSEVSIPEHMHALAAWNPITLEGFLVQMMLGNGMGFNWKGLYDVGLLDA
HHHHHHHHCCCHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEEC
HSGWRTRADDLSVTLKLTMLVGQWGLEHYRGRYYAKSRNIAIAAKAAYDAVFGSYDLLLM
CCCCCCCCCCEEEEEEEEEHHHHHCHHHHCCEEEEECCCEEEEEHHHHHHHHCCCCEEEE
PTLPCVATPIPAKDAPLAEVVQRAFEMTATTSPFDVTGHPAMTLPCGLSDGLPVGLMLIG
CCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEE
KDYAEATIYQAAAAFEADGDWKTF
CCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SIKRPNAEEVADLAASLHMNMTVEEAAEYLSLMGGMFDQYDIIDELPNPLPPVKYPRTP
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCC
GYKPMPKENKYNAWAIKTEVKGASSGKLAGRTVVLKDNVALAGVPMMNGSTTLEGFIPAA
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCEEEEEEEECCCCCCEEEEECCC
DATIVTRILDAGGTIVGKAVCEHFCLSGGSHTSDPAPVHNPLKMGYSAGGSSSGSAALVA
CHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEEEE
AGEVDMSIGGDQGGSIRIPASYCGIYGMKATHGLVPYTGVMPIESTIDHTGPMTANVKDN
ECCEEEEECCCCCCEEEECHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEECCCCCC
ALLLEVLAGADGLDPRQYAPKVAAYTESLGKGVKGLKIGVLKEGFSAPNMEQGVVSKVKA
CEEEHHHHCCCCCCHHHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHH
GAERFAKMGASVSEVSIPEHMHALAAWNPITLEGFLVQMMLGNGMGFNWKGLYDVGLLDA
HHHHHHHHCCCHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEEC
HSGWRTRADDLSVTLKLTMLVGQWGLEHYRGRYYAKSRNIAIAAKAAYDAVFGSYDLLLM
CCCCCCCCCCEEEEEEEEEHHHHHCHHHHCCEEEEECCCEEEEEHHHHHHHHCCCCEEEE
PTLPCVATPIPAKDAPLAEVVQRAFEMTATTSPFDVTGHPAMTLPCGLSDGLPVGLMLIG
CCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEE
KDYAEATIYQAAAAFEADGDWKTF
CCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2013568 [H]