Click here to switch to the map view.

The map label for this gene is queA [H]

Identifier: 146340922

GI number: 146340922

Start: 4184011

End: 4185105

Strand: Reverse

Name: queA [H]

Synonym: BRADO3993

Alternate gene names: 146340922

Gene position: 4185105-4184011 (Counterclockwise)

Preceding gene: 146340924

Following gene: 146340920

Centisome position: 56.13

GC content: 69.04

Gene sequence:

>1095_bases
ATGCGCACCGACCTGTTCGATTTCCATCTTCCGCCCGAGAACATCGCGCTGCGCCCGGCGAGCCCGCGCGAGGCGGCGCG
CATGCTCGTGGTGCAGGGCGATGGCGTGCTGCGCGACCGCACCATCGGCGATCTGCCGGACTGGCTGGAGCCGGGCGACC
AACTCGTCGTCAACGACACCAAGGTGATCGCAGCCCAGCTGTCCGGCCGCCGCATCAGCCGCGAGACCGAGGCCAAGATC
GAGGCGACCCTGATCAAGCGGCTCGACGGCTCGCGCTGGCAGGCGCTGGTCCGGCCGGCCAAGAAGCTCTTAGCCGGCGA
CACGATCCGCTTCGGCAATGAGGGCAAGGTCTGCCTGCTCGGCCATCTCGACGCGACCGTCGAGGCCAAGGGCGAGGAGG
GCGAGGTGACGCTGTCCTTCGTGTTCCACGGCCCGGCGCTCGATCAGGCGATCGCCGATCTCGGCCGGCCGCCGCTGCCG
CCCTACATCGCCGGCAAGCGGCCGGCCGACGAGCAGGACGCGCAGGACTATCAGACCATGTTCGCGGCCAAGGAGGGCGC
GGTGGCCGCGCCCACCGCCGGGTTGCACTTTACGCCCGCGCTGGAGCAGCGCCTGCGCGCGCGCGGCGTCGGGTTGCAGC
GCGTCACCCTGCATGTCGGCGCGGGAACGTTTCTCCCGGTCAAGGTCGAGGACACCGAAGGCCACCGCATGCACGCGGAG
TGGGGCAGCCTCACGCCGGAGACCGCCGCAGCGCTGAACCAGGCCAAAGCCGAAGGCGGCCGTATCGTCGCAGTCGGCAC
CACGTCGATGCGACTGCTGGAAAGCGCCGCGCTGCCCGACGGCACCATCGCGCCGTTCGAGGCGGAGACCTCGATCTTCA
TCACGCCGGGCTATCGCTTCCGAGCGGTCGACATCCTGATGACCAACTTCCATCTGCCGCGCTCGACCCTGTTCATGCTG
GTCTCCGCCTTTGCCGGCCTGGAGACGATGCAGGCGGCGTATGCGCATGCGATCGAGAGCGGCTACCGCTTCTATTCCTA
TGGCGATGCGTGCTTGTTGTTCCGGCAACCGGACGAGCGCGTGGTCGAGCCGTAG

Upstream 100 bases:

>100_bases
GCAAGGGCGGCGAAGCCCTCGATACGTTCATCGGTTGACCCGCCCGGCCGATGAGCGCCGTCACACTGAAAATGTTGAGC
CAAGAGCTGAGCCAAGAGCC

Downstream 100 bases:

>100_bases
GGCGCAAAATCGTAGGGTGGGCAAAGCGGCGCCGAAGGCGACGCGTGCCCACCGTCGATCAGCGCGTTGCGCGGCGAGAC
GGTGGGCACGGCGCCACTGC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MRTDLFDFHLPPENIALRPASPREAARMLVVQGDGVLRDRTIGDLPDWLEPGDQLVVNDTKVIAAQLSGRRISRETEAKI
EATLIKRLDGSRWQALVRPAKKLLAGDTIRFGNEGKVCLLGHLDATVEAKGEEGEVTLSFVFHGPALDQAIADLGRPPLP
PYIAGKRPADEQDAQDYQTMFAAKEGAVAAPTAGLHFTPALEQRLRARGVGLQRVTLHVGAGTFLPVKVEDTEGHRMHAE
WGSLTPETAAALNQAKAEGGRIVAVGTTSMRLLESAALPDGTIAPFEAETSIFITPGYRFRAVDILMTNFHLPRSTLFML
VSAFAGLETMQAAYAHAIESGYRFYSYGDACLLFRQPDERVVEP

Sequences:

>Translated_364_residues
MRTDLFDFHLPPENIALRPASPREAARMLVVQGDGVLRDRTIGDLPDWLEPGDQLVVNDTKVIAAQLSGRRISRETEAKI
EATLIKRLDGSRWQALVRPAKKLLAGDTIRFGNEGKVCLLGHLDATVEAKGEEGEVTLSFVFHGPALDQAIADLGRPPLP
PYIAGKRPADEQDAQDYQTMFAAKEGAVAAPTAGLHFTPALEQRLRARGVGLQRVTLHVGAGTFLPVKVEDTEGHRMHAE
WGSLTPETAAALNQAKAEGGRIVAVGTTSMRLLESAALPDGTIAPFEAETSIFITPGYRFRAVDILMTNFHLPRSTLFML
VSAFAGLETMQAAYAHAIESGYRFYSYGDACLLFRQPDERVVEP
>Mature_364_residues
MRTDLFDFHLPPENIALRPASPREAARMLVVQGDGVLRDRTIGDLPDWLEPGDQLVVNDTKVIAAQLSGRRISRETEAKI
EATLIKRLDGSRWQALVRPAKKLLAGDTIRFGNEGKVCLLGHLDATVEAKGEEGEVTLSFVFHGPALDQAIADLGRPPLP
PYIAGKRPADEQDAQDYQTMFAAKEGAVAAPTAGLHFTPALEQRLRARGVGLQRVTLHVGAGTFLPVKVEDTEGHRMHAE
WGSLTPETAAALNQAKAEGGRIVAVGTTSMRLLESAALPDGTIAPFEAETSIFITPGYRFRAVDILMTNFHLPRSTLFML
VSAFAGLETMQAAYAHAIESGYRFYSYGDACLLFRQPDERVVEP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=370, Percent_Identity=45.4054054054054, Blast_Score=268, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39650; Mature: 39650

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTDLFDFHLPPENIALRPASPREAARMLVVQGDGVLRDRTIGDLPDWLEPGDQLVVNDT
CCCCEEEEECCCCCEEECCCCHHHHEEEEEEECCCEEECCCCCCCCHHHCCCCEEEEECC
KVIAAQLSGRRISRETEAKIEATLIKRLDGSRWQALVRPAKKLLAGDTIRFGNEGKVCLL
EEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEE
GHLDATVEAKGEEGEVTLSFVFHGPALDQAIADLGRPPLPPYIAGKRPADEQDAQDYQTM
EECCCEEEECCCCCCEEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
FAAKEGAVAAPTAGLHFTPALEQRLRARGVGLQRVTLHVGAGTFLPVKVEDTEGHRMHAE
HHHCCCCCCCCCCCCEECHHHHHHHHHHCCCEEEEEEEECCCEEEEEEEECCCCCEEECC
WGSLTPETAAALNQAKAEGGRIVAVGTTSMRLLESAALPDGTIAPFEAETSIFITPGYRF
CCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCE
RAVDILMTNFHLPRSTLFMLVSAFAGLETMQAAYAHAIESGYRFYSYGDACLLFRQPDER
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEECCCHH
VVEP
CCCC
>Mature Secondary Structure
MRTDLFDFHLPPENIALRPASPREAARMLVVQGDGVLRDRTIGDLPDWLEPGDQLVVNDT
CCCCEEEEECCCCCEEECCCCHHHHEEEEEEECCCEEECCCCCCCCHHHCCCCEEEEECC
KVIAAQLSGRRISRETEAKIEATLIKRLDGSRWQALVRPAKKLLAGDTIRFGNEGKVCLL
EEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEE
GHLDATVEAKGEEGEVTLSFVFHGPALDQAIADLGRPPLPPYIAGKRPADEQDAQDYQTM
EECCCEEEECCCCCCEEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
FAAKEGAVAAPTAGLHFTPALEQRLRARGVGLQRVTLHVGAGTFLPVKVEDTEGHRMHAE
HHHCCCCCCCCCCCCEECHHHHHHHHHHCCCEEEEEEEECCCEEEEEEEECCCCCEEECC
WGSLTPETAAALNQAKAEGGRIVAVGTTSMRLLESAALPDGTIAPFEAETSIFITPGYRF
CCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCE
RAVDILMTNFHLPRSTLFMLVSAFAGLETMQAAYAHAIESGYRFYSYGDACLLFRQPDER
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEECCCHH
VVEP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA