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The map label for this gene is rhtB [H]

Identifier: 146340699

GI number: 146340699

Start: 3913926

End: 3914546

Strand: Reverse

Name: rhtB [H]

Synonym: BRADO3750

Alternate gene names: 146340699

Gene position: 3914546-3913926 (Counterclockwise)

Preceding gene: 146340701

Following gene: 146340698

Centisome position: 52.5

GC content: 66.99

Gene sequence:

>621_bases
ATGTCCACTGCCTTCCTGCTGACCTCGCTGATCGTGGTCGCCTCGCCCGGCACCGGCGTGCTCTATACGCTCGCAGTGGC
GCTGACGCGGGGCTCACGCATGAGCGTCGCCGCCGCGTTCGGCTGCACGCTCGGCATCATCCCGGCAATGCTCGCTGCGA
TCGTCGGCCTCGCGGCGGTGCTGCACACCAGCGCGCTTGCCTTTGCTGCGCTGAAATATTGCGGTGTCGTCTATCTGCTG
TACATGGCCTGGCAGACGCTCGGCGAGCGCGGCGCACTGTCGGTCGAGGCTGGAGCCAAGAAGGGCCAGACGCGGTCAGC
CGGACGCGTGGTGCTGACCGCCTTCCTGATCAACATCCTCAATCCCAAACTGTCGATCTTCTTCCTCGCCTTCCTGCCGC
AGTTCATCGCGGCTGACGAGGCGCATCCGCTCGCCGCCATGCTGGAGCTGTCGGCCACATTCATGGCGATGACGTTCGCG
GTGTTTGTGGTCTACGGGCTGTTCGCGGCGGCGGTTCGCGACCGGGTCGTCAGCCGTCCGCGGGTGATGGCGTGGCTGCG
CCGGGCGTTCGCCGGCGGTTTTGCTTTGCTCGGGGCGCGGCTGGCGCTGAGTGAGCGGTGA

Upstream 100 bases:

>100_bases
CTATCGGTCGCCGCGTGGTGTGAAATGCTGGTGTTCGTTGTTCAAATGAGCCGGCCGGCCCCTTCAAGGCACGGCGCCGG
GTACCTCTGGGAGCCCACCG

Downstream 100 bases:

>100_bases
GGACATTGCGCGGACATGACACGCAGCGCACCGGACCATGGAGACAAGCGTAGCCCGGATGAGCGGACGCTGCAGCCCGA
AGGGCGTAAGCGGTCGCGAC

Product: RhtB family amino acid efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSTAFLLTSLIVVASPGTGVLYTLAVALTRGSRMSVAAAFGCTLGIIPAMLAAIVGLAAVLHTSALAFAALKYCGVVYLL
YMAWQTLGERGALSVEAGAKKGQTRSAGRVVLTAFLINILNPKLSIFFLAFLPQFIAADEAHPLAAMLELSATFMAMTFA
VFVVYGLFAAAVRDRVVSRPRVMAWLRRAFAGGFALLGARLALSER

Sequences:

>Translated_206_residues
MSTAFLLTSLIVVASPGTGVLYTLAVALTRGSRMSVAAAFGCTLGIIPAMLAAIVGLAAVLHTSALAFAALKYCGVVYLL
YMAWQTLGERGALSVEAGAKKGQTRSAGRVVLTAFLINILNPKLSIFFLAFLPQFIAADEAHPLAAMLELSATFMAMTFA
VFVVYGLFAAAVRDRVVSRPRVMAWLRRAFAGGFALLGARLALSER
>Mature_205_residues
STAFLLTSLIVVASPGTGVLYTLAVALTRGSRMSVAAAFGCTLGIIPAMLAAIVGLAAVLHTSALAFAALKYCGVVYLLY
MAWQTLGERGALSVEAGAKKGQTRSAGRVVLTAFLINILNPKLSIFFLAFLPQFIAADEAHPLAAMLELSATFMAMTFAV
FVVYGLFAAAVRDRVVSRPRVMAWLRRAFAGGFALLGARLALSER

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=202, Percent_Identity=31.1881188118812, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788099, Length=163, Percent_Identity=27.6073619631902, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21722; Mature: 21591

Theoretical pI: Translated: 10.93; Mature: 10.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAFLLTSLIVVASPGTGVLYTLAVALTRGSRMSVAAAFGCTLGIIPAMLAAIVGLAAV
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHTSALAFAALKYCGVVYLLYMAWQTLGERGALSVEAGAKKGQTRSAGRVVLTAFLINIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
NPKLSIFFLAFLPQFIAADEAHPLAAMLELSATFMAMTFAVFVVYGLFAAAVRDRVVSRP
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
RVMAWLRRAFAGGFALLGARLALSER
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STAFLLTSLIVVASPGTGVLYTLAVALTRGSRMSVAAAFGCTLGIIPAMLAAIVGLAAV
CHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHTSALAFAALKYCGVVYLLYMAWQTLGERGALSVEAGAKKGQTRSAGRVVLTAFLINIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
NPKLSIFFLAFLPQFIAADEAHPLAAMLELSATFMAMTFAVFVVYGLFAAAVRDRVVSRP
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
RVMAWLRRAFAGGFALLGARLALSER
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]