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The map label for this gene is nifS [H]

Identifier: 146340497

GI number: 146340497

Start: 3731738

End: 3732901

Strand: Direct

Name: nifS [H]

Synonym: BRADO3539

Alternate gene names: 146340497

Gene position: 3731738-3732901 (Clockwise)

Preceding gene: 146340496

Following gene: 146340498

Centisome position: 50.05

GC content: 68.13

Gene sequence:

>1164_bases
ATGCGCGATCGCGTCTATCTCGATTGGAATGCCACCACGCCGCTGCGTCCCGAGGCGCGCGCGGCGATGGTTGCGGCGTT
CGACCTCGTCGGCAATCCGTCGTCGGTGCATGACGAGGGCCGCAAGGCACGGCGGGTGATGGAGGAGGCCCGCCCAGTGA
TCGCCCGCGGTCTTGGCACGGTGGCGCGCCGGGTCGTGTTCACCTCCGGCGGCACGGAGGCCAACACGCTGGCCCTGAGC
CCAGGGATGAAGACGCCGGCCGGACCGGTGGAGCGGCTCCTGGTATCGGCCGTGGAACATGCCTCGGTCCTGGCCGGCGG
CCGGTTCGCGCCGGCGGCGATTGAGACGATCCCGGTGGATCGGTCCGGCCTGGTCGACCCCGGCCGGCTCGAAGCGCTCC
TGGCCGGTGGTCCGCCGGCGCTGGTTTCGGTCATGGCCGCCAACAATGAGACCGGCGTGCTTCAACCGGTTTCGCAAGTC
GCTGAGATCGTTCGCCGGGCCGGCGGCCTGCTGCACGTCGACGCGATCCAGGCGTTCGGCAAAATTCCGCTCGATATCAA
CCAGATGGGCGCAAATCTTGTGAGCATTTCCGCGCACAAGATCGGTGGCCCCAAAGGGAGCGGCGCGCTGGTGCTGGGCG
ATGCTGTGGATGACGTCATCCCGGTGCTGCGGGGCGGCGGGCAGGAGCGTGGGCGGCGGGCGGGAACCGAGAATCTTGCC
GGGATCGCCGGGTTTGGCGCTGCGGCAGGCGCGGCTCTGACCGCCCTGGAGGCCGATCTTCCGCGGGTTACGGCGCTCCG
GGATCGCCTGGAGGCAGGTCTCCGCCAGACGGCTGGCGTTACGGTGTTCTCATCCGATGCGCCGCGGCTGCCCAATACCA
CTCTGTTCACGGCGCCCGGGATGAAGGCCGAGACCGCCGTGATCGGCTTCGATCTCGAAGGTATTTCAGTATCTTCGGGC
TCGGCCTGCTCTTCGGGCAAGGTCCAACCCTCTCACGTGCTGGAGGCGATGGGTGTCGGTCCTGCAGCCGCCCCGGGAGC
GGTGCGCCTCAGTCTGGGCTGGTCCACGACGTCGGCGGAGGTCGATCGGGCCGTCGAGGCTTGGCGAAAGCTGTCGATGA
CCCTACTTAAGGGACGTGGCGAAACAGCGCTTGAGCAATTCTAA

Upstream 100 bases:

>100_bases
TGAAAATGGCGACCACCGCGCCGGTCAGGCCGGTTCAGCAACCGGCTTGAGGCGCAGTTCCGATTGGCGTGGTCGCGCAA
GGTGGTAAGTAAGAGAGGCC

Downstream 100 bases:

>100_bases
GGGCGTGATTTCGTCTGCGAAACGTCGTAAGACGGTTCCGTAAGATGTCCACCGCGGTCCTTGAAACCGCGAGCGGAGGA
TGGAATGCCAGCCGTACAAG

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MRDRVYLDWNATTPLRPEARAAMVAAFDLVGNPSSVHDEGRKARRVMEEARPVIARGLGTVARRVVFTSGGTEANTLALS
PGMKTPAGPVERLLVSAVEHASVLAGGRFAPAAIETIPVDRSGLVDPGRLEALLAGGPPALVSVMAANNETGVLQPVSQV
AEIVRRAGGLLHVDAIQAFGKIPLDINQMGANLVSISAHKIGGPKGSGALVLGDAVDDVIPVLRGGGQERGRRAGTENLA
GIAGFGAAAGAALTALEADLPRVTALRDRLEAGLRQTAGVTVFSSDAPRLPNTTLFTAPGMKAETAVIGFDLEGISVSSG
SACSSGKVQPSHVLEAMGVGPAAAPGAVRLSLGWSTTSAEVDRAVEAWRKLSMTLLKGRGETALEQF

Sequences:

>Translated_387_residues
MRDRVYLDWNATTPLRPEARAAMVAAFDLVGNPSSVHDEGRKARRVMEEARPVIARGLGTVARRVVFTSGGTEANTLALS
PGMKTPAGPVERLLVSAVEHASVLAGGRFAPAAIETIPVDRSGLVDPGRLEALLAGGPPALVSVMAANNETGVLQPVSQV
AEIVRRAGGLLHVDAIQAFGKIPLDINQMGANLVSISAHKIGGPKGSGALVLGDAVDDVIPVLRGGGQERGRRAGTENLA
GIAGFGAAAGAALTALEADLPRVTALRDRLEAGLRQTAGVTVFSSDAPRLPNTTLFTAPGMKAETAVIGFDLEGISVSSG
SACSSGKVQPSHVLEAMGVGPAAAPGAVRLSLGWSTTSAEVDRAVEAWRKLSMTLLKGRGETALEQF
>Mature_387_residues
MRDRVYLDWNATTPLRPEARAAMVAAFDLVGNPSSVHDEGRKARRVMEEARPVIARGLGTVARRVVFTSGGTEANTLALS
PGMKTPAGPVERLLVSAVEHASVLAGGRFAPAAIETIPVDRSGLVDPGRLEALLAGGPPALVSVMAANNETGVLQPVSQV
AEIVRRAGGLLHVDAIQAFGKIPLDINQMGANLVSISAHKIGGPKGSGALVLGDAVDDVIPVLRGGGQERGRRAGTENLA
GIAGFGAAAGAALTALEADLPRVTALRDRLEAGLRQTAGVTVFSSDAPRLPNTTLFTAPGMKAETAVIGFDLEGISVSSG
SACSSGKVQPSHVLEAMGVGPAAAPGAVRLSLGWSTTSAEVDRAVEAWRKLSMTLLKGRGETALEQF

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=369, Percent_Identity=36.5853658536585, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=36.5617433414044, Blast_Score=190, Evalue=2e-48,
Organism=Escherichia coli, GI48994898, Length=377, Percent_Identity=35.2785145888594, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1787970, Length=377, Percent_Identity=23.6074270557029, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=371, Percent_Identity=35.8490566037736, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17533177, Length=330, Percent_Identity=32.7272727272727, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6319831, Length=369, Percent_Identity=36.0433604336043, Blast_Score=217, Evalue=3e-57,
Organism=Drosophila melanogaster, GI20129463, Length=382, Percent_Identity=34.8167539267016, Blast_Score=196, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 39712; Mature: 39712

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDRVYLDWNATTPLRPEARAAMVAAFDLVGNPSSVHDEGRKARRVMEEARPVIARGLGT
CCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VARRVVFTSGGTEANTLALSPGMKTPAGPVERLLVSAVEHASVLAGGRFAPAAIETIPVD
HHHHEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCC
RSGLVDPGRLEALLAGGPPALVSVMAANNETGVLQPVSQVAEIVRRAGGLLHVDAIQAFG
CCCCCCCHHHHEEECCCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEHHHHHHC
KIPLDINQMGANLVSISAHKIGGPKGSGALVLGDAVDDVIPVLRGGGQERGRRAGTENLA
CCCCCHHHHCCCEEEEEHHHCCCCCCCCCEEECCHHHHHHHHHHCCCHHHHHHCCCCCCC
GIAGFGAAAGAALTALEADLPRVTALRDRLEAGLRQTAGVTVFSSDAPRLPNTTLFTAPG
CHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEECCC
MKAETAVIGFDLEGISVSSGSACSSGKVQPSHVLEAMGVGPAAAPGAVRLSLGWSTTSAE
CCCCEEEEEEECCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCHHH
VDRAVEAWRKLSMTLLKGRGETALEQF
HHHHHHHHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MRDRVYLDWNATTPLRPEARAAMVAAFDLVGNPSSVHDEGRKARRVMEEARPVIARGLGT
CCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VARRVVFTSGGTEANTLALSPGMKTPAGPVERLLVSAVEHASVLAGGRFAPAAIETIPVD
HHHHEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCC
RSGLVDPGRLEALLAGGPPALVSVMAANNETGVLQPVSQVAEIVRRAGGLLHVDAIQAFG
CCCCCCCHHHHEEECCCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEHHHHHHC
KIPLDINQMGANLVSISAHKIGGPKGSGALVLGDAVDDVIPVLRGGGQERGRRAGTENLA
CCCCCHHHHCCCEEEEEHHHCCCCCCCCCEEECCHHHHHHHHHHCCCHHHHHHCCCCCCC
GIAGFGAAAGAALTALEADLPRVTALRDRLEAGLRQTAGVTVFSSDAPRLPNTTLFTAPG
CHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEECCC
MKAETAVIGFDLEGISVSSGSACSSGKVQPSHVLEAMGVGPAAAPGAVRLSLGWSTTSAE
CCCCEEEEEEECCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCHHH
VDRAVEAWRKLSMTLLKGRGETALEQF
HHHHHHHHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA