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The map label for this gene is sspA [H]

Identifier: 146340492

GI number: 146340492

Start: 3727228

End: 3727905

Strand: Direct

Name: sspA [H]

Synonym: BRADO3534

Alternate gene names: 146340492

Gene position: 3727228-3727905 (Clockwise)

Preceding gene: 146340491

Following gene: 146340495

Centisome position: 49.99

GC content: 66.81

Gene sequence:

>678_bases
ATGTCGCCGGTCCTGAAGCTGATCAGCCACAAGCTCTGCCCCTATGTGCAACGCGCCGTCATTGCGCTCAGCGAGAAGGG
CGTCGCCTTCGAGCGGATCGACATCGATCTCGCCAACAAGCCCGACTGGTTCCTGAAGATCTCGCCGCTCGGCAAGGTGC
CGGTGCTGGTGGTGCCCACGCCCCAGGGCGAGGTGGCGCTGTTCGAGAGCAACGTGATCTGCGAGTACATCGAGGACACG
CAAGCAGGCGGCAAGCTGCATCCGCTGGGCGCGCTGGCCCGCGCGCAGCACCGCGCCTGGATGGAGTTCGGCTCGGCGAT
CCTCGGCGACCTCTGGGGTCTGGAGACGACGCAGGATGCTCAGGTGTTCGCGGCCAAGCGCGAGGCGCTGACGGCGAAGT
TCGCCCGCGTCGAGGCGGCGCTGGGCGAGGGGCCGTACTTCGCCGGCGCGCCGTTCAGCCTGGTCGACGCGGTGTTCGCG
CCGAGCTTCCGTTACTTCGATGTGTTCGATGCATACGGCGATCTCGGCATCTTCGCCGTGACGCCGAAGGTGCGAGCCTG
GCGCGCTCAGCTCGCGCGGCGGCCGAGCGTGAAGACGGCTGTCAGCCACGAGTATCCGCAGCTGCTGCGCGCATTCCTGA
CGCGTCATGATGCGTATTTGTTGAAGCAGGCGGCGTAG

Upstream 100 bases:

>100_bases
GGTGATCGCGCGGCCCTGATTCATGCATCTGCATTCATCTTGACAGCTTCATGCAAAGGCATCTAGTTTGATGCATCTGC
ATCAAACTGCCGAGGATGTC

Downstream 100 bases:

>100_bases
GGACTTTGTTGTCGCACCGCGTGCTCGCGCCACACCCACAGCTGTCATCGCCCGGCTTGACCGGGCGATCCAGTACTCCG
AGCCACCGATGATCAGCCGA

Product: putative glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MSPVLKLISHKLCPYVQRAVIALSEKGVAFERIDIDLANKPDWFLKISPLGKVPVLVVPTPQGEVALFESNVICEYIEDT
QAGGKLHPLGALARAQHRAWMEFGSAILGDLWGLETTQDAQVFAAKREALTAKFARVEAALGEGPYFAGAPFSLVDAVFA
PSFRYFDVFDAYGDLGIFAVTPKVRAWRAQLARRPSVKTAVSHEYPQLLRAFLTRHDAYLLKQAA

Sequences:

>Translated_225_residues
MSPVLKLISHKLCPYVQRAVIALSEKGVAFERIDIDLANKPDWFLKISPLGKVPVLVVPTPQGEVALFESNVICEYIEDT
QAGGKLHPLGALARAQHRAWMEFGSAILGDLWGLETTQDAQVFAAKREALTAKFARVEAALGEGPYFAGAPFSLVDAVFA
PSFRYFDVFDAYGDLGIFAVTPKVRAWRAQLARRPSVKTAVSHEYPQLLRAFLTRHDAYLLKQAA
>Mature_224_residues
SPVLKLISHKLCPYVQRAVIALSEKGVAFERIDIDLANKPDWFLKISPLGKVPVLVVPTPQGEVALFESNVICEYIEDTQ
AGGKLHPLGALARAQHRAWMEFGSAILGDLWGLETTQDAQVFAAKREALTAKFARVEAALGEGPYFAGAPFSLVDAVFAP
SFRYFDVFDAYGDLGIFAVTPKVRAWRAQLARRPSVKTAVSHEYPQLLRAFLTRHDAYLLKQAA

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI4758484, Length=202, Percent_Identity=28.7128712871287, Blast_Score=104, Evalue=5e-23,
Organism=Homo sapiens, GI300360534, Length=196, Percent_Identity=29.0816326530612, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI38016131, Length=200, Percent_Identity=29, Blast_Score=95, Evalue=4e-20,
Organism=Homo sapiens, GI300360569, Length=193, Percent_Identity=29.5336787564767, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI300360532, Length=200, Percent_Identity=27.5, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI300360567, Length=194, Percent_Identity=27.8350515463918, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI300360571, Length=187, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=6e-17,
Organism=Escherichia coli, GI1789624, Length=161, Percent_Identity=31.6770186335404, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144449, Length=216, Percent_Identity=26.8518518518519, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI193207701, Length=207, Percent_Identity=27.536231884058, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21355763, Length=164, Percent_Identity=33.5365853658537, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21355779, Length=175, Percent_Identity=34.8571428571429, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21355775, Length=200, Percent_Identity=27, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355765, Length=105, Percent_Identity=37.1428571428571, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24660979, Length=164, Percent_Identity=28.6585365853659, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 24800; Mature: 24669

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPVLKLISHKLCPYVQRAVIALSEKGVAFERIDIDLANKPDWFLKISPLGKVPVLVVPT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEEECCCCCCCEEEEEC
PQGEVALFESNVICEYIEDTQAGGKLHPLGALARAQHRAWMEFGSAILGDLWGLETTQDA
CCCCEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
QVFAAKREALTAKFARVEAALGEGPYFAGAPFSLVDAVFAPSFRYFDVFDAYGDLGIFAV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEHHHCCCCCEEEEE
TPKVRAWRAQLARRPSVKTAVSHEYPQLLRAFLTRHDAYLLKQAA
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPVLKLISHKLCPYVQRAVIALSEKGVAFERIDIDLANKPDWFLKISPLGKVPVLVVPT
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEEECCCCCCCEEEEEC
PQGEVALFESNVICEYIEDTQAGGKLHPLGALARAQHRAWMEFGSAILGDLWGLETTQDA
CCCCEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
QVFAAKREALTAKFARVEAALGEGPYFAGAPFSLVDAVFAPSFRYFDVFDAYGDLGIFAV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEHHHCCCCCEEEEE
TPKVRAWRAQLARRPSVKTAVSHEYPQLLRAFLTRHDAYLLKQAA
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]