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The map label for this gene is prfB [H]

Identifier: 146340475

GI number: 146340475

Start: 3703620

End: 3704591

Strand: Direct

Name: prfB [H]

Synonym: BRADO3516

Alternate gene names: 146340475

Gene position: 3703620-3704591 (Clockwise)

Preceding gene: 146340473

Following gene: 146340476

Centisome position: 49.67

GC content: 65.33

Gene sequence:

>972_bases
ATGCAGGAGCGGACCTCGCTGGAGGATTCGCTGACCGGCATTGGCAAGGTCGAGCGTGAGCTCAGCGACAACATCGAGAT
GATCGAGCTCGGCGAGGCCGAGAACGACGCCGGTGTGGTCGCGGAGGCCGAGAACGCGCTCAAGGAGCTGAAGAAGGAGG
TCGCCCGGCGCGAGCTCGAGGCTCTGCTGTCCGGCGAGGCCGACAAGTTCGACACCTATCTCGAGGTTCACGCCGGGGCC
GGTGGCACCGAGAGCCAGGACTGGGCCTCGATGCTGCTGCGCATGTACACGCGCTGGGCCGAGACTCACGGCTTCAAGGT
CGAGTATCTCGAAGAGTCGCTTGGCGAAGAGGCCGGCATCAAGTCGGCGACCATCCAGATCTCCGGCCACAATGCCTATG
GCTGGCTGAAGACGGAAGCCGGCGTGCATCGCCTGGTGCGAATCTCGCCGTTCGATTCGAACGCACGCCGCCACACCTCG
TTCTCGTCGGTGCAGATCTTCCCGGTCATCGACGACTCGATCAAGATCGACATCAAGGAGTCCGACGTCCGCGTCGATAC
CATGCGGTCGGGCGGCGCCGGCGGTCAGCACGTCAACAAGACCGAATCGGCGGTGCGTCTGACGCATATCCCGACCGGTG
TCGCGGTGGTCTGCCAGGCCGGCCGCTCGCAGCACAAGAACCGTGCCCAGGCCTGGGACATGTTGCGTGCGCGCCTCTAC
GAGATCGAGCTGAAGAAGCGCGAGGAGAAGGCGGCCGCGGACCAGGCCGCCAAGACCGAGATCGGCTGGGGCCACCAGAT
CCGCTCCTATGTGCTGCAGCCCTATCAGATGGTGAAGGACCTGCGCACGGGCGTGCAGACCTCCGACACTTCGGGCGTGC
TCAACGGCGATCTCGACGAGTTCATGGCGGCCACGCTGGCCCAGCGCGCCTTCGGCACCTCGCCGGGAGCCGTGATCGAG
GATGTGGACTAG

Upstream 100 bases:

>100_bases
CATCTTTGACGTCGAGACATCGACGGCAAGGCTGGCCGAGCTGAACAAGCTCGCTGAAGATCCCAATCTCTGGAACGACC
CCCAGAAGGCGCAACGGCTG

Downstream 100 bases:

>100_bases
CCGGTGGCGCGCGTCGCGTTCATCGGACTCGGCCGCATGGGCCATGGCATGGCCGGACGCTATCTCGACGCCGGCCATGC
GGTCGCGGTCTGGAATCGCA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MQERTSLEDSLTGIGKVERELSDNIEMIELGEAENDAGVVAEAENALKELKKEVARRELEALLSGEADKFDTYLEVHAGA
GGTESQDWASMLLRMYTRWAETHGFKVEYLEESLGEEAGIKSATIQISGHNAYGWLKTEAGVHRLVRISPFDSNARRHTS
FSSVQIFPVIDDSIKIDIKESDVRVDTMRSGGAGGQHVNKTESAVRLTHIPTGVAVVCQAGRSQHKNRAQAWDMLRARLY
EIELKKREEKAAADQAAKTEIGWGHQIRSYVLQPYQMVKDLRTGVQTSDTSGVLNGDLDEFMAATLAQRAFGTSPGAVIE
DVD

Sequences:

>Translated_323_residues
MQERTSLEDSLTGIGKVERELSDNIEMIELGEAENDAGVVAEAENALKELKKEVARRELEALLSGEADKFDTYLEVHAGA
GGTESQDWASMLLRMYTRWAETHGFKVEYLEESLGEEAGIKSATIQISGHNAYGWLKTEAGVHRLVRISPFDSNARRHTS
FSSVQIFPVIDDSIKIDIKESDVRVDTMRSGGAGGQHVNKTESAVRLTHIPTGVAVVCQAGRSQHKNRAQAWDMLRARLY
EIELKKREEKAAADQAAKTEIGWGHQIRSYVLQPYQMVKDLRTGVQTSDTSGVLNGDLDEFMAATLAQRAFGTSPGAVIE
DVD
>Mature_323_residues
MQERTSLEDSLTGIGKVERELSDNIEMIELGEAENDAGVVAEAENALKELKKEVARRELEALLSGEADKFDTYLEVHAGA
GGTESQDWASMLLRMYTRWAETHGFKVEYLEESLGEEAGIKSATIQISGHNAYGWLKTEAGVHRLVRISPFDSNARRHTS
FSSVQIFPVIDDSIKIDIKESDVRVDTMRSGGAGGQHVNKTESAVRLTHIPTGVAVVCQAGRSQHKNRAQAWDMLRARLY
EIELKKREEKAAADQAAKTEIGWGHQIRSYVLQPYQMVKDLRTGVQTSDTSGVLNGDLDEFMAATLAQRAFGTSPGAVIE
DVD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=271, Percent_Identity=38.3763837638376, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI34577120, Length=262, Percent_Identity=38.5496183206107, Blast_Score=141, Evalue=7e-34,
Organism=Homo sapiens, GI166795305, Length=174, Percent_Identity=39.6551724137931, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI2367172, Length=305, Percent_Identity=54.0983606557377, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1787462, Length=235, Percent_Identity=42.1276595744681, Blast_Score=181, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI17542784, Length=203, Percent_Identity=34.9753694581281, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=285, Percent_Identity=39.2982456140351, Blast_Score=151, Evalue=1e-37,
Organism=Drosophila melanogaster, GI19921226, Length=300, Percent_Identity=34.6666666666667, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 35636; Mature: 35636

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQERTSLEDSLTGIGKVERELSDNIEMIELGEAENDAGVVAEAENALKELKKEVARRELE
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
ALLSGEADKFDTYLEVHAGAGGTESQDWASMLLRMYTRWAETHGFKVEYLEESLGEEAGI
HHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHCCC
KSATIQISGHNAYGWLKTEAGVHRLVRISPFDSNARRHTSFSSVQIFPVIDDSIKIDIKE
CEEEEEEECCCCCEEEECHHCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEC
SDVRVDTMRSGGAGGQHVNKTESAVRLTHIPTGVAVVCQAGRSQHKNRAQAWDMLRARLY
CCEEEEECCCCCCCCCCCCCHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIELKKREEKAAADQAAKTEIGWGHQIRSYVLQPYQMVKDLRTGVQTSDTSGVLNGDLDE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
FMAATLAQRAFGTSPGAVIEDVD
HHHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure
MQERTSLEDSLTGIGKVERELSDNIEMIELGEAENDAGVVAEAENALKELKKEVARRELE
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
ALLSGEADKFDTYLEVHAGAGGTESQDWASMLLRMYTRWAETHGFKVEYLEESLGEEAGI
HHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHCCC
KSATIQISGHNAYGWLKTEAGVHRLVRISPFDSNARRHTSFSSVQIFPVIDDSIKIDIKE
CEEEEEEECCCCCEEEECHHCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEEEEC
SDVRVDTMRSGGAGGQHVNKTESAVRLTHIPTGVAVVCQAGRSQHKNRAQAWDMLRARLY
CCEEEEECCCCCCCCCCCCCHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIELKKREEKAAADQAAKTEIGWGHQIRSYVLQPYQMVKDLRTGVQTSDTSGVLNGDLDE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
FMAATLAQRAFGTSPGAVIEDVD
HHHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA