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The map label for this gene is dnaB [H]

Identifier: 146340279

GI number: 146340279

Start: 3497226

End: 3498728

Strand: Reverse

Name: dnaB [H]

Synonym: BRADO3302

Alternate gene names: 146340279

Gene position: 3498728-3497226 (Counterclockwise)

Preceding gene: 146340280

Following gene: 146340278

Centisome position: 46.92

GC content: 65.07

Gene sequence:

>1503_bases
TTGACCGATTCGAACGTTCTTAAGCTGGCTCCGACGGACGCCGCAACGCCGGCCTATCGCAGCGCGCCTCACAACATCGA
GGCGGAGCAGGGTCTGCTCGGCGCGATCCTCGTCAACAACGACGCGTTCTATCGCGTGTCTGATTTCCTCGAGCCGAAGC
ATTTCTACGAGCCGCTGCATCAGACGATCTTCGAGACGACGGCGAGCCTCATCCGCATGGGCAAGATCGCCACGCCCGTG
ACGTTGAAGACGTTCCTGCCCGCGGACGTCGATCTTGGCGGCATGACCGTCGGCCAGTATCTGGCGCGCCTCGCCGCCGA
GGCGACGACGATCATCAACGCGCAGGACTATGGCCGCACCATCTACGATCTGTCGTTGCGCCGTGACCTCATCGGCATCG
GCGAGGACATGGTCAACGTCGCCTATGACGCGCCGGTCGATTTCGCGCCGCGCAACCAGATCGAGGATGCCGAGCGCAGG
CTGTACGAGCTCGCCGAGTCCGGCCGCTACGACGGCGGTTTCCAGAAGTTCTCGCAGGCGCTGACCACCGCGCTCGACAT
GGCCGCGAAGGCGTTCCAGCGCGACGGCAAGCTGTCGGGCATCTCGACCGGCCTGCGCGATCTCGACGCCAAGATGGGCG
GCCTGCAGCACTCCGACCTGATCATCCTCGCCGGCCGTCCCGGCATGGGCAAGACCTCGCTCGCCACCAATATCGCCTAC
AACGTCGCGCAGGCCTATGTTCCGGAACTGCAGCCCGACGGCACCTACAAGGCGGCGCAGGGCGGCGTGGTCGGGTTCTT
CTCGTGCGAAATGAGCGGCGAACAGCTCGCTACCCGCATCATCGCCGAGCGTTCCGGCATTCCCTCGAGCCACATCCGCC
GCGGCGGCATCACCGAGGCCGATTTCGAAAAGCTGCGCGAGTGCTCGATCGAGCTGCAATCGCTGCCGTTCTATGTCGAC
GAAACCGGCGGCATCTCGATCTCGCAGGTGATGGCGCGCGCCCGCCGGCTGAAGCGGCAAAGGGGGCTCGACCTCCTGGT
GATCGACTACATCCAGCTGCTCGCCGGCTCCGGCAAGCGTGGCAGCGACAACCGCGTGCAGGAAATCACCGAGATCACCA
CCAGCCTGAAGGCGCTGGCGAAGGAGCTCAACGTTCCGATCATCGCGCTGTCGCAGCTGTCGCGTCAGGTCGAAAACCGC
GACGACAAGCGGCCGCAGCTGTCGGACCTGCGTGAATCCGGCTCGATCGAGCAGGACGCCGACGTCGTGCTGTTCGTGTA
CCGCGGCGAATACTACCTGGCTGCCAAGGAGCCGCGCCCCGGCACCGAGGAGCACCAGAAGTGGCAGACCGAAATGGAAC
TGGCGCTGGGCAAGGCCGAAGTGATCATCGGCAAGCAGCGTCACGGTCCGACCGGCACCGTCGAGCTGCACTTCGACGCC
TCGGTCACGCGCTTCGGCGACCTCGCCCATGACGGTATGCCGCCGCAGCATCACGACTATTGA

Upstream 100 bases:

>100_bases
TTACCGGACGGTGAATAGCGACCACGTTTCGCTTCCGCTTCGGACGCGACTGGCGCTTTATCCCCTCCCCTGCAAAGCTG
CTAAACACGCATCCATGGCA

Downstream 100 bases:

>100_bases
CCGTGTGCTGTTGAACAGATGCGCCGCGGCGCGTAAAAGCGCCGCATGAGTGTTCCCGAACCGAAACCCGCCGAGCCCTC
TGCGTCGGCGGCCGATGCGA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MTDSNVLKLAPTDAATPAYRSAPHNIEAEQGLLGAILVNNDAFYRVSDFLEPKHFYEPLHQTIFETTASLIRMGKIATPV
TLKTFLPADVDLGGMTVGQYLARLAAEATTIINAQDYGRTIYDLSLRRDLIGIGEDMVNVAYDAPVDFAPRNQIEDAERR
LYELAESGRYDGGFQKFSQALTTALDMAAKAFQRDGKLSGISTGLRDLDAKMGGLQHSDLIILAGRPGMGKTSLATNIAY
NVAQAYVPELQPDGTYKAAQGGVVGFFSCEMSGEQLATRIIAERSGIPSSHIRRGGITEADFEKLRECSIELQSLPFYVD
ETGGISISQVMARARRLKRQRGLDLLVIDYIQLLAGSGKRGSDNRVQEITEITTSLKALAKELNVPIIALSQLSRQVENR
DDKRPQLSDLRESGSIEQDADVVLFVYRGEYYLAAKEPRPGTEEHQKWQTEMELALGKAEVIIGKQRHGPTGTVELHFDA
SVTRFGDLAHDGMPPQHHDY

Sequences:

>Translated_500_residues
MTDSNVLKLAPTDAATPAYRSAPHNIEAEQGLLGAILVNNDAFYRVSDFLEPKHFYEPLHQTIFETTASLIRMGKIATPV
TLKTFLPADVDLGGMTVGQYLARLAAEATTIINAQDYGRTIYDLSLRRDLIGIGEDMVNVAYDAPVDFAPRNQIEDAERR
LYELAESGRYDGGFQKFSQALTTALDMAAKAFQRDGKLSGISTGLRDLDAKMGGLQHSDLIILAGRPGMGKTSLATNIAY
NVAQAYVPELQPDGTYKAAQGGVVGFFSCEMSGEQLATRIIAERSGIPSSHIRRGGITEADFEKLRECSIELQSLPFYVD
ETGGISISQVMARARRLKRQRGLDLLVIDYIQLLAGSGKRGSDNRVQEITEITTSLKALAKELNVPIIALSQLSRQVENR
DDKRPQLSDLRESGSIEQDADVVLFVYRGEYYLAAKEPRPGTEEHQKWQTEMELALGKAEVIIGKQRHGPTGTVELHFDA
SVTRFGDLAHDGMPPQHHDY
>Mature_499_residues
TDSNVLKLAPTDAATPAYRSAPHNIEAEQGLLGAILVNNDAFYRVSDFLEPKHFYEPLHQTIFETTASLIRMGKIATPVT
LKTFLPADVDLGGMTVGQYLARLAAEATTIINAQDYGRTIYDLSLRRDLIGIGEDMVNVAYDAPVDFAPRNQIEDAERRL
YELAESGRYDGGFQKFSQALTTALDMAAKAFQRDGKLSGISTGLRDLDAKMGGLQHSDLIILAGRPGMGKTSLATNIAYN
VAQAYVPELQPDGTYKAAQGGVVGFFSCEMSGEQLATRIIAERSGIPSSHIRRGGITEADFEKLRECSIELQSLPFYVDE
TGGISISQVMARARRLKRQRGLDLLVIDYIQLLAGSGKRGSDNRVQEITEITTSLKALAKELNVPIIALSQLSRQVENRD
DKRPQLSDLRESGSIEQDADVVLFVYRGEYYLAAKEPRPGTEEHQKWQTEMELALGKAEVIIGKQRHGPTGTVELHFDAS
VTRFGDLAHDGMPPQHHDY

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=492, Percent_Identity=36.3821138211382, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 55008; Mature: 54877

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSNVLKLAPTDAATPAYRSAPHNIEAEQGLLGAILVNNDAFYRVSDFLEPKHFYEPLH
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEHHHHCCHHHHHHHHH
QTIFETTASLIRMGKIATPVTLKTFLPADVDLGGMTVGQYLARLAAEATTIINAQDYGRT
HHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEECCHHHCCE
IYDLSLRRDLIGIGEDMVNVAYDAPVDFAPRNQIEDAERRLYELAESGRYDGGFQKFSQA
EEEHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
LTTALDMAAKAFQRDGKLSGISTGLRDLDAKMGGLQHSDLIILAGRPGMGKTSLATNIAY
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHH
NVAQAYVPELQPDGTYKAAQGGVVGFFSCEMSGEQLATRIIAERSGIPSSHIRRGGITEA
HHHHHHCCCCCCCCCEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHCCCCCHH
DFEKLRECSIELQSLPFYVDETGGISISQVMARARRLKRQRGLDLLVIDYIQLLAGSGKR
HHHHHHHHHCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
GSDNRVQEITEITTSLKALAKELNVPIIALSQLSRQVENRDDKRPQLSDLRESGSIEQDA
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCC
DVVLFVYRGEYYLAAKEPRPGTEEHQKWQTEMELALGKAEVIIGKQRHGPTGTVELHFDA
CEEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEECC
SVTRFGDLAHDGMPPQHHDY
CHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TDSNVLKLAPTDAATPAYRSAPHNIEAEQGLLGAILVNNDAFYRVSDFLEPKHFYEPLH
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEHHHHCCHHHHHHHHH
QTIFETTASLIRMGKIATPVTLKTFLPADVDLGGMTVGQYLARLAAEATTIINAQDYGRT
HHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEECCHHHCCE
IYDLSLRRDLIGIGEDMVNVAYDAPVDFAPRNQIEDAERRLYELAESGRYDGGFQKFSQA
EEEHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
LTTALDMAAKAFQRDGKLSGISTGLRDLDAKMGGLQHSDLIILAGRPGMGKTSLATNIAY
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHH
NVAQAYVPELQPDGTYKAAQGGVVGFFSCEMSGEQLATRIIAERSGIPSSHIRRGGITEA
HHHHHHCCCCCCCCCEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHCCCCCHH
DFEKLRECSIELQSLPFYVDETGGISISQVMARARRLKRQRGLDLLVIDYIQLLAGSGKR
HHHHHHHHHCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
GSDNRVQEITEITTSLKALAKELNVPIIALSQLSRQVENRDDKRPQLSDLRESGSIEQDA
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCC
DVVLFVYRGEYYLAAKEPRPGTEEHQKWQTEMELALGKAEVIIGKQRHGPTGTVELHFDA
CEEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEECC
SVTRFGDLAHDGMPPQHHDY
CHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA