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The map label for this gene is lipB

Identifier: 146340240

GI number: 146340240

Start: 3463056

End: 3463778

Strand: Reverse

Name: lipB

Synonym: BRADO3259

Alternate gene names: 146340240

Gene position: 3463778-3463056 (Counterclockwise)

Preceding gene: 146340241

Following gene: 146340238

Centisome position: 46.45

GC content: 68.6

Gene sequence:

>723_bases
ATGGTTAACGGCCGCGAAACGCTCGACCTCACCCCCTTCGCCGGCCCTCCGGGCAATGTGGCGGCGTGGCGCATCTCCGA
TGCGCTGGTCGACTATGCCGAGGCGACGGCGTTCATGGAGGCCCGTGCTGCGGCCATCGCGGCCCATCAGGCCGAGGAGC
TGGTCTGGCTGCTCGAGCATCCGCCGGTCTACACCTCCGGCACCTCGGGCAAGCCCGAGGATCTGCGCGATCCGCGCTTC
CCTTTCATCGCCACCGGGCGCGGCGGCCAGGTCACCTATCACGGCCCCGGCCAGCGCGTGGCCTATGTCATGCTCGACCT
CAAGCGTCGCCGGCCCGACGTGCGGGCCTATGTCGCGGCGCTGGAGGAGACCATCATTCGCACGCTCGACGCGTTCAACG
TGCGCGGCGAGCGGCGGGAGGACCGCGTCGGCGTCTGGGTGAAACGGCCCGACAAGGGCGAAGGCCATGAAGACAAGATC
GCCGCGATCGGCGTGAGGCTCAAACGCTGGGTGACGTTTCACGGCATCGCCATCAATGTCGAGCCGGACCTGTCGCACTT
CCAGGCGATCGTGCCCTGCGGCGTGACCGATCCGCGCTACGGCGTCACCAGCCTGGTCGATCTCGGCCTGCCCGTGACCA
TGGCCGATGTCGATGTGGCGCTCCGGCAGGCGTTCGAGAGCGTGTTCGGCGCGACCCGTGCGATCCTGCCGGAAACCGCA
TGA

Upstream 100 bases:

>100_bases
ACCATCAGGTCGACTGTCACTTTATCGAGGGTTGACACTGCGGGAGCACCGAAGTCACTGGATTCCGTTACCCCAGACAT
CACCATGTTGTGGTTAGCCA

Downstream 100 bases:

>100_bases
AGTCAGGCGGCGCCGCGCTGCAGGGGCACGTCGAGCCTGGCGGCAATATATCGACGCTCCTGGCTGAGGCCGCCGAAGCC
ATAGGCATCCGCCTTGACCT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MVNGRETLDLTPFAGPPGNVAAWRISDALVDYAEATAFMEARAAAIAAHQAEELVWLLEHPPVYTSGTSGKPEDLRDPRF
PFIATGRGGQVTYHGPGQRVAYVMLDLKRRRPDVRAYVAALEETIIRTLDAFNVRGERREDRVGVWVKRPDKGEGHEDKI
AAIGVRLKRWVTFHGIAINVEPDLSHFQAIVPCGVTDPRYGVTSLVDLGLPVTMADVDVALRQAFESVFGATRAILPETA

Sequences:

>Translated_240_residues
MVNGRETLDLTPFAGPPGNVAAWRISDALVDYAEATAFMEARAAAIAAHQAEELVWLLEHPPVYTSGTSGKPEDLRDPRF
PFIATGRGGQVTYHGPGQRVAYVMLDLKRRRPDVRAYVAALEETIIRTLDAFNVRGERREDRVGVWVKRPDKGEGHEDKI
AAIGVRLKRWVTFHGIAINVEPDLSHFQAIVPCGVTDPRYGVTSLVDLGLPVTMADVDVALRQAFESVFGATRAILPETA
>Mature_240_residues
MVNGRETLDLTPFAGPPGNVAAWRISDALVDYAEATAFMEARAAAIAAHQAEELVWLLEHPPVYTSGTSGKPEDLRDPRF
PFIATGRGGQVTYHGPGQRVAYVMLDLKRRRPDVRAYVAALEETIIRTLDAFNVRGERREDRVGVWVKRPDKGEGHEDKI
AAIGVRLKRWVTFHGIAINVEPDLSHFQAIVPCGVTDPRYGVTSLVDLGLPVTMADVDVALRQAFESVFGATRAILPETA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=179, Percent_Identity=37.9888268156425, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI87081767, Length=141, Percent_Identity=47.5177304964539, Blast_Score=134, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6323268, Length=179, Percent_Identity=32.9608938547486, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21355483, Length=182, Percent_Identity=39.010989010989, Blast_Score=107, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_BRASO (A4YT30)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001205288.1
- ProteinModelPortal:   A4YT30
- SMR:   A4YT30
- STRING:   A4YT30
- GeneID:   5118461
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO3259
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- ProtClustDB:   PRK14341
- BioCyc:   BSP376:BRADO3259-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 26353; Mature: 26353

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNGRETLDLTPFAGPPGNVAAWRISDALVDYAEATAFMEARAAAIAAHQAEELVWLLEH
CCCCCCCEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
PPVYTSGTSGKPEDLRDPRFPFIATGRGGQVTYHGPGQRVAYVMLDLKRRRPDVRAYVAA
CCEEECCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCEEEHHEEEHHHCCCHHHHHHHH
LEETIIRTLDAFNVRGERREDRVGVWVKRPDKGEGHEDKIAAIGVRLKRWVTFHGIAINV
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHEEEEEEEEEEE
EPDLSHFQAIVPCGVTDPRYGVTSLVDLGLPVTMADVDVALRQAFESVFGATRAILPETA
CCCHHHHHEEECCCCCCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVNGRETLDLTPFAGPPGNVAAWRISDALVDYAEATAFMEARAAAIAAHQAEELVWLLEH
CCCCCCCEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
PPVYTSGTSGKPEDLRDPRFPFIATGRGGQVTYHGPGQRVAYVMLDLKRRRPDVRAYVAA
CCEEECCCCCCCCCCCCCCCCEEEECCCCEEEECCCCCEEEHHEEEHHHCCCHHHHHHHH
LEETIIRTLDAFNVRGERREDRVGVWVKRPDKGEGHEDKIAAIGVRLKRWVTFHGIAINV
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHEEEEEEEEEEE
EPDLSHFQAIVPCGVTDPRYGVTSLVDLGLPVTMADVDVALRQAFESVFGATRAILPETA
CCCHHHHHEEECCCCCCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA