Click here to switch to the map view.

The map label for this gene is prpB [C]

Identifier: 146340231

GI number: 146340231

Start: 3455714

End: 3456580

Strand: Direct

Name: prpB [C]

Synonym: BRADO3250

Alternate gene names: 146340231

Gene position: 3455714-3456580 (Clockwise)

Preceding gene: 146340230

Following gene: 146340232

Centisome position: 46.34

GC content: 67.59

Gene sequence:

>867_bases
ATGACTTTCCGTCTGCGTCGGGAGGCGCTTCGCACGATCCTCACCAGCGATCGCTGCGTGAACCCCGGCTCCGTCTACGA
CGCGATCTCGATCCGCATCGCCGAGGATCTCGGCTTTCCGCTCGGCATGTTCGGCGGCTCGGCGGCGTCGCTGGCCATTC
TCGGCGATCCCGACATCGCGCTGATCACGCTTACCGAGCTCGCCGAGCAGATGCGGCGGATGTCGCGCGCGGCGGTGCTG
CCGGTGCTGGTCGATGCCGACCACGGCTACGGCAATGCGATGAACGTCCGCCGCACGGTGCAGGAGCTGGAGGCCGCCGG
GGCCGCCGGCCTCACCATCGAGGACACGTCGCTGCCGCAGGCCTATGGCGCGGCCAAGCCGCAGCTGATCTCGTTGGATG
AAGGCGTCGGCAAGGTGAAGGCCGCGCTCGATGGCCGCGGCGATTCCTCGCTGGTGATCCTCGGGCGCACTGGCGCTGTC
TCGATCACCTCGCTCGACGACGCGATTGCTCGCGCCAGGGTCTATGAGACCTGCGGCGTCGACGGTCTTTTCTTCACCGG
CATCACGACGCGCGATCAGCTCGATGCGATCGCGGCGGCGACGAACTTGCCGATCGTGCTGGGCGGTGCGCCGGAAGAGA
TGTCCGACCTCGGCTATCTCGCCTCGCGCCGCGTCCGGATCGCGCTGCAGGGACATGCGCCGATCGCAGCCGCGACGCAG
GCGGTGTACGACACGCTGAAGGCGCTGCGTGATGGCGTGTCACCAAAACAGCTCAAGGGACTGCCGTCGGCCGAGCTGAC
GTCGCGCGTGATGCGTGAGGCCGACGTCAAGAAACGGCTGCGCGATTTTCTGGGGCTCGGATCGTGA

Upstream 100 bases:

>100_bases
CGCCGTCATTGCGAGCGAAGCGACGCAATCCAGGGCTCGGCACGCCACTCTGGATTGCTTCGTCGCTTCGCTCCTCGCGA
TGCCGAGAGAGGAGCCCGGA

Downstream 100 bases:

>100_bases
GCGATCTGACCATGGTCCATGTCATCGTGCGCGGCCGCGTTCAGGGCGTCGGCTATCGAGCCTGGGTCGAGGATCAGGCC
ATTCTGAACGGCCTCGGAGG

Product: putative carboxyvinyl-carboxyphosphonate phosphorylmutase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTFRLRREALRTILTSDRCVNPGSVYDAISIRIAEDLGFPLGMFGGSAASLAILGDPDIALITLTELAEQMRRMSRAAVL
PVLVDADHGYGNAMNVRRTVQELEAAGAAGLTIEDTSLPQAYGAAKPQLISLDEGVGKVKAALDGRGDSSLVILGRTGAV
SITSLDDAIARARVYETCGVDGLFFTGITTRDQLDAIAAATNLPIVLGGAPEEMSDLGYLASRRVRIALQGHAPIAAATQ
AVYDTLKALRDGVSPKQLKGLPSAELTSRVMREADVKKRLRDFLGLGS

Sequences:

>Translated_288_residues
MTFRLRREALRTILTSDRCVNPGSVYDAISIRIAEDLGFPLGMFGGSAASLAILGDPDIALITLTELAEQMRRMSRAAVL
PVLVDADHGYGNAMNVRRTVQELEAAGAAGLTIEDTSLPQAYGAAKPQLISLDEGVGKVKAALDGRGDSSLVILGRTGAV
SITSLDDAIARARVYETCGVDGLFFTGITTRDQLDAIAAATNLPIVLGGAPEEMSDLGYLASRRVRIALQGHAPIAAATQ
AVYDTLKALRDGVSPKQLKGLPSAELTSRVMREADVKKRLRDFLGLGS
>Mature_287_residues
TFRLRREALRTILTSDRCVNPGSVYDAISIRIAEDLGFPLGMFGGSAASLAILGDPDIALITLTELAEQMRRMSRAAVLP
VLVDADHGYGNAMNVRRTVQELEAAGAAGLTIEDTSLPQAYGAAKPQLISLDEGVGKVKAALDGRGDSSLVILGRTGAVS
ITSLDDAIARARVYETCGVDGLFFTGITTRDQLDAIAAATNLPIVLGGAPEEMSDLGYLASRRVRIALQGHAPIAAATQA
VYDTLKALRDGVSPKQLKGLPSAELTSRVMREADVKKRLRDFLGLGS

Specific function: Catalyzes the decarboxylation of oxaloacetate into pyruvate. Seems to play a role in maintaining cellular concentrations of bicarbonate and pyruvate

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Oxaloacetate decarboxylase family

Homologues:

Organism=Escherichia coli, GI1786525, Length=254, Percent_Identity=33.0708661417323, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OADC_BRASO (A4YT21)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001205279.1
- STRING:   A4YT21
- GeneID:   5117148
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO3250
- eggNOG:   COG2513
- HOGENOM:   HBG728656
- OMA:   VDADHGY
- ProtClustDB:   CLSK868955
- BioCyc:   BSP376:BRADO3250-MONOMER
- HAMAP:   MF_01299
- InterPro:   IPR013785
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =4.1.1.3

Molecular weight: Translated: 30407; Mature: 30276

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: BINDING 47-47 BINDING 156-156 BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRLRREALRTILTSDRCVNPGSVYDAISIRIAEDLGFPLGMFGGSAASLAILGDPDIA
CCCHHHHHHHHHHHHCCCCCCCCCHHHHEEEEEHHHCCCCHHHCCCCCEEEEEECCCCEE
LITLTELAEQMRRMSRAAVLPVLVDADHGYGNAMNVRRTVQELEAAGAAGLTIEDTSLPQ
EEEHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCH
AYGAAKPQLISLDEGVGKVKAALDGRGDSSLVILGRTGAVSITSLDDAIARARVYETCGV
HHCCCCCEEEECCCCCHHHHHHHCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHCCC
DGLFFTGITTRDQLDAIAAATNLPIVLGGAPEEMSDLGYLASRRVRIALQGHAPIAAATQ
CCEEEECCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHH
AVYDTLKALRDGVSPKQLKGLPSAELTSRVMREADVKKRLRDFLGLGS
HHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TFRLRREALRTILTSDRCVNPGSVYDAISIRIAEDLGFPLGMFGGSAASLAILGDPDIA
CCHHHHHHHHHHHHCCCCCCCCCHHHHEEEEEHHHCCCCHHHCCCCCEEEEEECCCCEE
LITLTELAEQMRRMSRAAVLPVLVDADHGYGNAMNVRRTVQELEAAGAAGLTIEDTSLPQ
EEEHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCH
AYGAAKPQLISLDEGVGKVKAALDGRGDSSLVILGRTGAVSITSLDDAIARARVYETCGV
HHCCCCCEEEECCCCCHHHHHHHCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHCCC
DGLFFTGITTRDQLDAIAAATNLPIVLGGAPEEMSDLGYLASRRVRIALQGHAPIAAATQ
CCEEEECCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHH
AVYDTLKALRDGVSPKQLKGLPSAELTSRVMREADVKKRLRDFLGLGS
HHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA