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The map label for this gene is zraS [H]

Identifier: 146340226

GI number: 146340226

Start: 3447763

End: 3449250

Strand: Reverse

Name: zraS [H]

Synonym: BRADO3245

Alternate gene names: 146340226

Gene position: 3449250-3447763 (Counterclockwise)

Preceding gene: 146340227

Following gene: 146340225

Centisome position: 46.26

GC content: 68.21

Gene sequence:

>1488_bases
TTGCGCGCTCAGGCATCCCTTGTCATTGCGGACCAATCGATGCGCTCCCTACGCTCGCGGCTGTCGGCGCTCTGGCTCAT
GCTGGCAGTCTCCGGCGCGGCGACGGCCTATCTTCTGGTCGAGTCGTTTCAGCAATCGTCGCAGGCGCGCGTCGCGCGCG
CGCAAGATCTCGTGGTTCGCGGCTGCCGCGATATCGCAGACCGCTATCAGTTCTTCGTCGCGGGCTGGGGCGGCGCGCCG
ATCGATGATCGCATCAAGCAGGACCTCATTCCCGTGGTGCAGTCCGCGCTGGCGGCATCGCCGGGCGTCGAGGGCGGCAT
CTGGCAGGCCGAGCATGGCTCGCTTGCCTATTCCTATCCGACCTATGAGGGCACGGGCCCGAAAACCGATCTCCCCGCAG
CAGAGCTCAGCGTCATTCAGCAGGTCAATGGCGACGCCCAGCGCAGCGGCCGTCCCGCGACGACGGAAAACGCCGGACGC
TCGCAGACCCTGATCGTCCATGCCTGCCCGCTGCGCGGGCCGCTGCCGAACGTCACGGCATGGACGATGACGCGCGCGGC
CACCGCGGAGGGTCCGGCCTACGCGCATCTGCTGACTGGTCTCTCGGTGCTGGCGCTCACGCTGCTTGGCTCGGCCATCT
GGCTGGCTTTCATCCTGGCCTCGTGGTCGCGCCGTATCGCCATTATCGAGCATGCGCTTGGCGTGGCGCCCGCAGCCGCC
GACCTTCCGCAGCTGCCGCACACCGGCGCGCGCGAGCTCGATCGCCTGGTCGATGCCGTCAACGATGCCGGCCACCGGCT
CGCAGCGGAGCGCGAAAGGGCAACGGCGGCTGAGCGGCTTGCCGCCGTCGGCCGTCTCTCGGCGAACCTCGCCCACGAGA
TCCGCAACCCGATCGCGGCGATGCGATTGAAAGCCGAGAATGCGCTCGCCTCGCCCGATCCCACGCGCAAGGGCCGCGCG
CTCGAAGCGATCCTGCAACAGGTCGCGCGGCTCGATGCGTTGTTGCGTGATCTGCTCGCCATGACCCAGGCGAGCCGGCC
CGCCACGCGTGCTGTCGATCTCGCGGCCTTCCTGCACGCGACGATCGAGACGCATCGCGAGCTGGCCGCGCGTCGCGGAC
TGGAATTGCACATGCGCGCACCCTCCGCCACCGAGGCCGCCTGCTTCGATCCTGTCCAGATGCAGCGTGCGCTCGACAAC
CTGATCCTTAACGCGATCCAGAATGCGCCGGAGCGAAGCGAGATCGTCGTGGCCGCCGAGATCCAGGACGCGCGACTGCT
CTTTCGCGTCATCGACAGCGGGCCCGGCGTTGATGCCCGCATCAAGGATCGCCTGTTCGAACCCTTCGTCAGCAGCCGCG
CCGAAGGCACCGGCCTCGGCCTCGCCATCGTGCGCGAGATCGCCCGCGCCCACCATGGCGAGGTGCAACTCGCACACACT
CGTCACGGCTCCAGCTTCGAGATCTCCCTCCCATGGCCACCATCCTGA

Upstream 100 bases:

>100_bases
GCGCCACTGCCACGGTGAGCCGGTAGCGCGGGAACCATTGTTGCAGATCAGGCGTTCCTTTCCGTGATGACGTGCACCGA
TCCGTGTCCTGCGGAAGGCT

Downstream 100 bases:

>100_bases
TCGTCGACGACGATGCGGCGCTGCGCGACGGTCTCGCGGAGACGCTGACCGATCTCGGTCACGACACGCTAACGGCTGCA
TCGGGGCGCGAGGCGATCGG

Product: putative two-component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MRAQASLVIADQSMRSLRSRLSALWLMLAVSGAATAYLLVESFQQSSQARVARAQDLVVRGCRDIADRYQFFVAGWGGAP
IDDRIKQDLIPVVQSALAASPGVEGGIWQAEHGSLAYSYPTYEGTGPKTDLPAAELSVIQQVNGDAQRSGRPATTENAGR
SQTLIVHACPLRGPLPNVTAWTMTRAATAEGPAYAHLLTGLSVLALTLLGSAIWLAFILASWSRRIAIIEHALGVAPAAA
DLPQLPHTGARELDRLVDAVNDAGHRLAAERERATAAERLAAVGRLSANLAHEIRNPIAAMRLKAENALASPDPTRKGRA
LEAILQQVARLDALLRDLLAMTQASRPATRAVDLAAFLHATIETHRELAARRGLELHMRAPSATEAACFDPVQMQRALDN
LILNAIQNAPERSEIVVAAEIQDARLLFRVIDSGPGVDARIKDRLFEPFVSSRAEGTGLGLAIVREIARAHHGEVQLAHT
RHGSSFEISLPWPPS

Sequences:

>Translated_495_residues
MRAQASLVIADQSMRSLRSRLSALWLMLAVSGAATAYLLVESFQQSSQARVARAQDLVVRGCRDIADRYQFFVAGWGGAP
IDDRIKQDLIPVVQSALAASPGVEGGIWQAEHGSLAYSYPTYEGTGPKTDLPAAELSVIQQVNGDAQRSGRPATTENAGR
SQTLIVHACPLRGPLPNVTAWTMTRAATAEGPAYAHLLTGLSVLALTLLGSAIWLAFILASWSRRIAIIEHALGVAPAAA
DLPQLPHTGARELDRLVDAVNDAGHRLAAERERATAAERLAAVGRLSANLAHEIRNPIAAMRLKAENALASPDPTRKGRA
LEAILQQVARLDALLRDLLAMTQASRPATRAVDLAAFLHATIETHRELAARRGLELHMRAPSATEAACFDPVQMQRALDN
LILNAIQNAPERSEIVVAAEIQDARLLFRVIDSGPGVDARIKDRLFEPFVSSRAEGTGLGLAIVREIARAHHGEVQLAHT
RHGSSFEISLPWPPS
>Mature_495_residues
MRAQASLVIADQSMRSLRSRLSALWLMLAVSGAATAYLLVESFQQSSQARVARAQDLVVRGCRDIADRYQFFVAGWGGAP
IDDRIKQDLIPVVQSALAASPGVEGGIWQAEHGSLAYSYPTYEGTGPKTDLPAAELSVIQQVNGDAQRSGRPATTENAGR
SQTLIVHACPLRGPLPNVTAWTMTRAATAEGPAYAHLLTGLSVLALTLLGSAIWLAFILASWSRRIAIIEHALGVAPAAA
DLPQLPHTGARELDRLVDAVNDAGHRLAAERERATAAERLAAVGRLSANLAHEIRNPIAAMRLKAENALASPDPTRKGRA
LEAILQQVARLDALLRDLLAMTQASRPATRAVDLAAFLHATIETHRELAARRGLELHMRAPSATEAACFDPVQMQRALDN
LILNAIQNAPERSEIVVAAEIQDARLLFRVIDSGPGVDARIKDRLFEPFVSSRAEGTGLGLAIVREIARAHHGEVQLAHT
RHGSSFEISLPWPPS

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=216, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1790861, Length=246, Percent_Identity=32.1138211382114, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1786783, Length=262, Percent_Identity=30.9160305343511, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788549, Length=234, Percent_Identity=27.3504273504274, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790300, Length=242, Percent_Identity=30.1652892561983, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789808, Length=303, Percent_Identity=29.7029702970297, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788393, Length=257, Percent_Identity=28.4046692607004, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790346, Length=221, Percent_Identity=28.0542986425339, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082128, Length=218, Percent_Identity=27.9816513761468, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788279, Length=261, Percent_Identity=26.4367816091954, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790551, Length=300, Percent_Identity=28, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1787894, Length=224, Percent_Identity=27.2321428571429, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1786600, Length=216, Percent_Identity=25, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1786912, Length=253, Percent_Identity=26.4822134387352, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789403, Length=217, Percent_Identity=26.2672811059908, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI145693157, Length=261, Percent_Identity=24.5210727969349, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87081816, Length=332, Percent_Identity=28.0120481927711, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53181; Mature: 53181

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS50109 HIS_KIN ; PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAQASLVIADQSMRSLRSRLSALWLMLAVSGAATAYLLVESFQQSSQARVARAQDLVVR
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GCRDIADRYQFFVAGWGGAPIDDRIKQDLIPVVQSALAASPGVEGGIWQAEHGSLAYSYP
HHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCEEEECC
TYEGTGPKTDLPAAELSVIQQVNGDAQRSGRPATTENAGRSQTLIVHACPLRGPLPNVTA
CCCCCCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHH
WTMTRAATAEGPAYAHLLTGLSVLALTLLGSAIWLAFILASWSRRIAIIEHALGVAPAAA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DLPQLPHTGARELDRLVDAVNDAGHRLAAERERATAAERLAAVGRLSANLAHEIRNPIAA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRLKAENALASPDPTRKGRALEAILQQVARLDALLRDLLAMTQASRPATRAVDLAAFLHA
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TIETHRELAARRGLELHMRAPSATEAACFDPVQMQRALDNLILNAIQNAPERSEIVVAAE
HHHHHHHHHHHCCCEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
IQDARLLFRVIDSGPGVDARIKDRLFEPFVSSRAEGTGLGLAIVREIARAHHGEVQLAHT
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
RHGSSFEISLPWPPS
CCCCEEEEECCCCCC
>Mature Secondary Structure
MRAQASLVIADQSMRSLRSRLSALWLMLAVSGAATAYLLVESFQQSSQARVARAQDLVVR
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GCRDIADRYQFFVAGWGGAPIDDRIKQDLIPVVQSALAASPGVEGGIWQAEHGSLAYSYP
HHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCEEEECC
TYEGTGPKTDLPAAELSVIQQVNGDAQRSGRPATTENAGRSQTLIVHACPLRGPLPNVTA
CCCCCCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHH
WTMTRAATAEGPAYAHLLTGLSVLALTLLGSAIWLAFILASWSRRIAIIEHALGVAPAAA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DLPQLPHTGARELDRLVDAVNDAGHRLAAERERATAAERLAAVGRLSANLAHEIRNPIAA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRLKAENALASPDPTRKGRALEAILQQVARLDALLRDLLAMTQASRPATRAVDLAAFLHA
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TIETHRELAARRGLELHMRAPSATEAACFDPVQMQRALDNLILNAIQNAPERSEIVVAAE
HHHHHHHHHHHCCCEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
IQDARLLFRVIDSGPGVDARIKDRLFEPFVSSRAEGTGLGLAIVREIARAHHGEVQLAHT
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
RHGSSFEISLPWPPS
CCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]