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The map label for this gene is hisI

Identifier: 146339980

GI number: 146339980

Start: 3186452

End: 3186844

Strand: Direct

Name: hisI

Synonym: BRADO2985

Alternate gene names: 146339980

Gene position: 3186452-3186844 (Clockwise)

Preceding gene: 146339979

Following gene: 146339981

Centisome position: 42.73

GC content: 65.39

Gene sequence:

>393_bases
TTGGCCTTCCTCCCGAAATTCGATTCGGCAGGTCTCGTCACCTGCGTGACCACCGACGCCGTGAGCGGCGACGTGCTGAT
GGTCGCGCACATGAACGACGAGGCGCTGCGCAAGACCATCGCGACCGGCGAGGCCTGGTATTTCAGCCGCTCGCGCAATG
CGCTGTGGCGCAAGGGCGAGAGCTCGGGCCAGACCCAGCGCGTGGTCGAGATGCGCACCGATTGCGACCAGGATGCGGTG
TGGTTGCGTGTCGAGCAGCGCGGCGCAGCCTGTCACACCGGCCGGCATTCCTGCTTCTATCGCAAGGTCGAGATGGCGGG
CGAGGGCGGGGCACGGCTTGTTTTCGTCGATGCCGACCGGCTGTTCGATCCGGCTGCTGTGTATCGCAAATAG

Upstream 100 bases:

>100_bases
GGTGCGCTCGCCGCAACGCTGAGCGCAGCATATTTCGGCTGTTACAGGCCTCGATCAGAGAAACTCCGTGTCTTCATCCT
CAGCTGAGCGCGAGGAGGGA

Downstream 100 bases:

>100_bases
GTGGCCCAGAGAGCCACGCGCGTGCGGGCCGGTGGGTCATAGGCCTGCATCCGGAGATTTCCGGTCTGGTTCATTGGCCT
TGTCAGGCCTGTCAAATTAA

Product: phosphoribosyl-AMP cyclohydrolase

Products: NA

Alternate protein names: PRA-CH

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MAFLPKFDSAGLVTCVTTDAVSGDVLMVAHMNDEALRKTIATGEAWYFSRSRNALWRKGESSGQTQRVVEMRTDCDQDAV
WLRVEQRGAACHTGRHSCFYRKVEMAGEGGARLVFVDADRLFDPAAVYRK

Sequences:

>Translated_130_residues
MAFLPKFDSAGLVTCVTTDAVSGDVLMVAHMNDEALRKTIATGEAWYFSRSRNALWRKGESSGQTQRVVEMRTDCDQDAV
WLRVEQRGAACHTGRHSCFYRKVEMAGEGGARLVFVDADRLFDPAAVYRK
>Mature_129_residues
AFLPKFDSAGLVTCVTTDAVSGDVLMVAHMNDEALRKTIATGEAWYFSRSRNALWRKGESSGQTQRVVEMRTDCDQDAVW
LRVEQRGAACHTGRHSCFYRKVEMAGEGGARLVFVDADRLFDPAAVYRK

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PRA-CH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=89, Percent_Identity=44.9438202247191, Blast_Score=90, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6319819, Length=90, Percent_Identity=38.8888888888889, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS3_BRASO (A4YSB5)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001205028.1
- ProteinModelPortal:   A4YSB5
- SMR:   A4YSB5
- STRING:   A4YSB5
- GeneID:   5120073
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO2985
- eggNOG:   COG0139
- HOGENOM:   HBG294308
- OMA:   LWLKGES
- ProtClustDB:   PRK00051
- BioCyc:   BSP376:BRADO2985-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01021
- InterPro:   IPR002496
- ProDom:   PD002610

Pfam domain/function: PF01502 PRA-CH

EC number: =3.5.4.19

Molecular weight: Translated: 14524; Mature: 14392

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFLPKFDSAGLVTCVTTDAVSGDVLMVAHMNDEALRKTIATGEAWYFSRSRNALWRKGE
CCCCCCCCCCCEEEEEEECCCCCCEEEEEECCHHHHHHHHCCCCEEEEECCCCCEECCCC
SSGQTQRVVEMRTDCDQDAVWLRVEQRGAACHTGRHSCFYRKVEMAGEGGARLVFVDADR
CCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCEEEEEEHHH
LFDPAAVYRK
CCCCHHHCCC
>Mature Secondary Structure 
AFLPKFDSAGLVTCVTTDAVSGDVLMVAHMNDEALRKTIATGEAWYFSRSRNALWRKGE
CCCCCCCCCCEEEEEEECCCCCCEEEEEECCHHHHHHHHCCCCEEEEECCCCCEECCCC
SSGQTQRVVEMRTDCDQDAVWLRVEQRGAACHTGRHSCFYRKVEMAGEGGARLVFVDADR
CCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCEEEEEEHHH
LFDPAAVYRK
CCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA