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The map label for this gene is dapE [H]

Identifier: 146339693

GI number: 146339693

Start: 2889220

End: 2890623

Strand: Direct

Name: dapE [H]

Synonym: BRADO2689

Alternate gene names: 146339693

Gene position: 2889220-2890623 (Clockwise)

Preceding gene: 146339692

Following gene: 146339694

Centisome position: 38.75

GC content: 63.96

Gene sequence:

>1404_bases
ATGGCCAATGCGCAGCTTCAATCCGTTCTCGACCACATCGATGCCGATTTCGACAACAGCCTGGACCGTCTGTTCCAGCT
GATCCGCATCAAGTCCATCTCGGCCGATCCGGCCTTTACCGACGATTGCAAGGCGGCGGCCGATCATCTCGCCAAGGATC
TCGCAACGCTCGGCTTCGCCACCGAGGTGCGTCCGACGGCAGGACACCCGGCCGTGGTGGGAAAGAGCAACGGTCATGGC
GCGGCCAAACCGCACGTGCTGTTCTATGGCCATTATGACGTACAGCCGGTCGATCCGCTCGATCTCTGGCACCGTCCGCC
GTTCGAGCCCGTCGTCACCGATCATGCCGACGGCCGCAAGATCATCGTCGCGCGCGGCGCCGAGGACGACAAGGGCCAGC
TGATGACCTTCGTCGAGGCCTGCCGCGCCTGGAAGAAGGTGACGGGATCGCTGCCGCTCGGCATCACCATGCTGATCGAG
GGCGAGGAGGAGGTCGGCTCGAAGAACTTCGTGCCGTTCCTCGAGGCCAACAAGGATGAGTTCAAAGCCGACTTCGCGCT
CGTCTGCGACACCGGCATGTGGGACCCGAACACGCCGGCGATCACGACATCGCTGCGCGGTCTTCTCTATGAAGAGCTCA
AGATCAAGGCCGCCAATCGCGACTTGCATTCGGGCGTGTTCGGTGGCGGCGCGCGCAATCCGATCCGGGTGCTGACCGAG
GTGCTCGGCGGTCTGTTCGACGCCGACGGCCGCATCACCATTCCCGGCTTCTACGACGGCGTGAAGGACGTGCCGCCGGA
AGTCCTCGAGCAGTGGAAGAAGCTGAACCTGACGCCGGAGAGCTTCCTCAAGCCGATCGGGTTGTCGATCCCCGCCGGCG
AGAAGGATCGCCTGCTGATCGAGCAGATCTCGTCGCGCCCGACCTGCGACATCAACGGCATCTGGGGCGGCTATATCGGC
GAGGGCTCGAAGACTGTCATTCCGTCGCTGGCCCATGCGAAGGTTTCATTCCGCCTCGTCGAAGGCCAGGACCCGTTGAA
GATCCGCAAGGCGTTCCGCGACTACGTATCGGCGCGCATCCCCGGCGACTGCTCGGTCGAGTTCGGCGATCACTCGGCGG
GTGCCGCGATCGCGCTCGACTGGAGCATGAAGCCGCTCGCCGCCGCCAAGCAGGCGCTGACCGAGGAGTGGGGCAAGGAG
ACCATCCTGATGGGCTCAGGTGCCTCGATCCCGATCGTCACCGACTTCAAGAAGACGCTCGGCCTCGACTCGCTCCTGGT
CGGTTTCGGTCTCGACGACGACAACATCCATTCGCCGAACGAGAAGTACGACCTCAAGAGCTACCACAAGGGCATCCGCT
CCTGGGCCCGCATCCTCGCAGCGCTGGCGGAGATGCCGCGTTAG

Upstream 100 bases:

>100_bases
TCTTCGCCAGCGTGATGCTCGCGGCATCACGCTGGTCCGGACCGCGCAACGGGGCTACAAGCAAGCCAACGCTCGTCACT
TGCGAAGGGATCCCGTGGCC

Downstream 100 bases:

>100_bases
CCAAGGAACTCTCGCGGCCGCGTCTTGAAGACTGCCAGTCGGTCGCTGACGATGTTGTGAGGGGGTGCGACAGAGAAGCC
CTTTCTCGCCCGGTCAGGGT

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MANAQLQSVLDHIDADFDNSLDRLFQLIRIKSISADPAFTDDCKAAADHLAKDLATLGFATEVRPTAGHPAVVGKSNGHG
AAKPHVLFYGHYDVQPVDPLDLWHRPPFEPVVTDHADGRKIIVARGAEDDKGQLMTFVEACRAWKKVTGSLPLGITMLIE
GEEEVGSKNFVPFLEANKDEFKADFALVCDTGMWDPNTPAITTSLRGLLYEELKIKAANRDLHSGVFGGGARNPIRVLTE
VLGGLFDADGRITIPGFYDGVKDVPPEVLEQWKKLNLTPESFLKPIGLSIPAGEKDRLLIEQISSRPTCDINGIWGGYIG
EGSKTVIPSLAHAKVSFRLVEGQDPLKIRKAFRDYVSARIPGDCSVEFGDHSAGAAIALDWSMKPLAAAKQALTEEWGKE
TILMGSGASIPIVTDFKKTLGLDSLLVGFGLDDDNIHSPNEKYDLKSYHKGIRSWARILAALAEMPR

Sequences:

>Translated_467_residues
MANAQLQSVLDHIDADFDNSLDRLFQLIRIKSISADPAFTDDCKAAADHLAKDLATLGFATEVRPTAGHPAVVGKSNGHG
AAKPHVLFYGHYDVQPVDPLDLWHRPPFEPVVTDHADGRKIIVARGAEDDKGQLMTFVEACRAWKKVTGSLPLGITMLIE
GEEEVGSKNFVPFLEANKDEFKADFALVCDTGMWDPNTPAITTSLRGLLYEELKIKAANRDLHSGVFGGGARNPIRVLTE
VLGGLFDADGRITIPGFYDGVKDVPPEVLEQWKKLNLTPESFLKPIGLSIPAGEKDRLLIEQISSRPTCDINGIWGGYIG
EGSKTVIPSLAHAKVSFRLVEGQDPLKIRKAFRDYVSARIPGDCSVEFGDHSAGAAIALDWSMKPLAAAKQALTEEWGKE
TILMGSGASIPIVTDFKKTLGLDSLLVGFGLDDDNIHSPNEKYDLKSYHKGIRSWARILAALAEMPR
>Mature_466_residues
ANAQLQSVLDHIDADFDNSLDRLFQLIRIKSISADPAFTDDCKAAADHLAKDLATLGFATEVRPTAGHPAVVGKSNGHGA
AKPHVLFYGHYDVQPVDPLDLWHRPPFEPVVTDHADGRKIIVARGAEDDKGQLMTFVEACRAWKKVTGSLPLGITMLIEG
EEEVGSKNFVPFLEANKDEFKADFALVCDTGMWDPNTPAITTSLRGLLYEELKIKAANRDLHSGVFGGGARNPIRVLTEV
LGGLFDADGRITIPGFYDGVKDVPPEVLEQWKKLNLTPESFLKPIGLSIPAGEKDRLLIEQISSRPTCDINGIWGGYIGE
GSKTVIPSLAHAKVSFRLVEGQDPLKIRKAFRDYVSARIPGDCSVEFGDHSAGAAIALDWSMKPLAAAKQALTEEWGKET
ILMGSGASIPIVTDFKKTLGLDSLLVGFGLDDDNIHSPNEKYDLKSYHKGIRSWARILAALAEMPR

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=480, Percent_Identity=29.375, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI21071039, Length=483, Percent_Identity=29.6066252587992, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI271398277, Length=322, Percent_Identity=30.1242236024845, Blast_Score=148, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17563146, Length=476, Percent_Identity=31.5126050420168, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI193211059, Length=474, Percent_Identity=28.2700421940928, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321159, Length=482, Percent_Identity=29.253112033195, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6319758, Length=451, Percent_Identity=24.1685144124169, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24585880, Length=486, Percent_Identity=30.8641975308642, Blast_Score=199, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 50856; Mature: 50725

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANAQLQSVLDHIDADFDNSLDRLFQLIRIKSISADPAFTDDCKAAADHLAKDLATLGFA
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
TEVRPTAGHPAVVGKSNGHGAAKPHVLFYGHYDVQPVDPLDLWHRPPFEPVVTDHADGRK
CCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCE
IIVARGAEDDKGQLMTFVEACRAWKKVTGSLPLGITMLIEGEEEVGSKNFVPFLEANKDE
EEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHCCCCCCCEEEECCCHH
FKADFALVCDTGMWDPNTPAITTSLRGLLYEELKIKAANRDLHSGVFGGGARNPIRVLTE
HCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHH
VLGGLFDADGRITIPGFYDGVKDVPPEVLEQWKKLNLTPESFLKPIGLSIPAGEKDRLLI
HHHHHCCCCCCEECCCHHCCHHCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHH
EQISSRPTCDINGIWGGYIGEGSKTVIPSLAHAKVSFRLVEGQDPLKIRKAFRDYVSARI
HHHCCCCCEEECCCCCCCCCCCCCCCCCCHHCCEEEEEEECCCCHHHHHHHHHHHHHCCC
PGDCSVEFGDHSAGAAIALDWSMKPLAAAKQALTEEWGKETILMGSGASIPIVTDFKKTL
CCCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEECHHHHH
GLDSLLVGFGLDDDNIHSPNEKYDLKSYHKGIRSWARILAALAEMPR
CHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANAQLQSVLDHIDADFDNSLDRLFQLIRIKSISADPAFTDDCKAAADHLAKDLATLGFA
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
TEVRPTAGHPAVVGKSNGHGAAKPHVLFYGHYDVQPVDPLDLWHRPPFEPVVTDHADGRK
CCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCE
IIVARGAEDDKGQLMTFVEACRAWKKVTGSLPLGITMLIEGEEEVGSKNFVPFLEANKDE
EEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHCCCCCCCEEEECCCHH
FKADFALVCDTGMWDPNTPAITTSLRGLLYEELKIKAANRDLHSGVFGGGARNPIRVLTE
HCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHH
VLGGLFDADGRITIPGFYDGVKDVPPEVLEQWKKLNLTPESFLKPIGLSIPAGEKDRLLI
HHHHHCCCCCCEECCCHHCCHHCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHH
EQISSRPTCDINGIWGGYIGEGSKTVIPSLAHAKVSFRLVEGQDPLKIRKAFRDYVSARI
HHHCCCCCEEECCCCCCCCCCCCCCCCCCHHCCEEEEEEECCCCHHHHHHHHHHHHHCCC
PGDCSVEFGDHSAGAAIALDWSMKPLAAAKQALTEEWGKETILMGSGASIPIVTDFKKTL
CCCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEECHHHHH
GLDSLLVGFGLDDDNIHSPNEKYDLKSYHKGIRSWARILAALAEMPR
CHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA