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The map label for this gene is gatA [H]

Identifier: 146339595

GI number: 146339595

Start: 2771313

End: 2772665

Strand: Direct

Name: gatA [H]

Synonym: BRADO2587

Alternate gene names: 146339595

Gene position: 2771313-2772665 (Clockwise)

Preceding gene: 146339594

Following gene: 146339597

Centisome position: 37.17

GC content: 70.36

Gene sequence:

>1353_bases
ATGACCGCTGCCAATCCGACCCTCGCCGCGCTCGCCGACGATCTCGCCGCGGGACGCACGACCTCGCGCAAGCTGGTCGA
GGACTGTCTCGTCCGCATCGCCGATCCGGCCGGCGAAGGCCAGCGCGCCTTCATCCACGTCGACAAGGACGCGGCGCTTT
CGGCCGCCGACGGCATGGACGCGCTGCGCAAGGCCAATGCCGCGCCGTCGGCGTTCGCGGGCATTCCCGTGTCGATCAAG
GACCTGTTCGACATCAAGGGTCAGGTGACCCGCGCCGGCTCGCGCGCGCTGGAGGATTCGGCGCCGGCCGAAGCGGATGC
GCCCGCGGTGGCGCGGCTGCGCAAGGCCGGCTTCGTCGTGATCGGCCGCACCAACATGACCGAGTTCGCCTATTCCGGCA
TCGGCATCAATCCGCATTACGGCACGCCGAAGAGCGCGTGGAAGCGCGAGGTCGGCTATGTGCCCGGCGGCTCGTCCTCG
GGCGCCGCGGTCTCGGTCGCCGACCGCATGGCCTATGGCGCGCTCGGCACCGATACCGGCGGCTCCTGCCGCATCCCTGC
GGCGTTCAACGGCATCACCGGCTACAAGCCGACCCAGGCGCGCGTGCCGCTCGACGGCGGCGTGCCGCTGTCGTCGACGC
TCGACAGCTTCGGCCCGCTCGCCAACACCGTGGCCTGCTGCGCGGTGCTGGACTCCGTGCTGGCTGACGAGCCGATCCGG
CCGCTGGCGTCACGTCCCGTGAAGGGGCTGCGGCTCGCGGTGCCGACGACCATCGTGCTCGACGAGCTCGATGCCGAGGT
GGCCGAAACGTTCGAGCGGGCGCTGGACACTCTCGCGAGACAGGGCGCGCTGATCGAGCGCATCGAATTTCCCGAGTTTC
TCGATGTCGGCGTCATCGGCATGAAGGGCGGCTTTGCCGCCGCCGAGAGCTACGCGTGGCATCGCTTCCTGTTGACCGCC
AAGGGCGACGTCTACGACCCCCGCGTCTCCGTGCGCATCCTGCGCGGCGAGGCGATCACAGTGCCCGACTACATCGAGAT
GCTCAACGCCCGCCGCTCCCTGGTGACGCGCGCCGCCGCGCGCATCGCGCCCTACGACGCGCTGGTGATGCCGACCACGG
CCAACGCGCCGCCGAAAATCGCCGACCTCGCCGACGACGCCGCCTTCGCGCGGGAAAACATCCGTGCGCTGCGCAACTGC
ACATTCATCAACATGATCGACGGCTGCGCCATCTCGCTGCCGGCCCATCGCCACGGCGAGGTCCCGGTCGGCCTGATGCT
GGCGCAGTCCGGCGGCAACGACCGCAAGCTGCTGGAGATCGCGGCGGGGATCGAGGGCGTCGTGCGGGGGTGA

Upstream 100 bases:

>100_bases
GGCGCTGGATGCGCACGGCAAGTCGCTGAGTTCCGCGCTGCTCGATCTCGATATTTCGGCTGATGCCGCTGACCCAAGTC
TAGACCCAAGTTAGGATGCC

Downstream 100 bases:

>100_bases
GGCGGCAGCAAGCGATCAGGGATTGTGGGGCGGGGCAGCGATCAAGGACAGTGGTGTCGCCTTGGCGGCGCTCGCCTGTG
CCTCGTCGATGAGGCCGGCT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MTAANPTLAALADDLAAGRTTSRKLVEDCLVRIADPAGEGQRAFIHVDKDAALSAADGMDALRKANAAPSAFAGIPVSIK
DLFDIKGQVTRAGSRALEDSAPAEADAPAVARLRKAGFVVIGRTNMTEFAYSGIGINPHYGTPKSAWKREVGYVPGGSSS
GAAVSVADRMAYGALGTDTGGSCRIPAAFNGITGYKPTQARVPLDGGVPLSSTLDSFGPLANTVACCAVLDSVLADEPIR
PLASRPVKGLRLAVPTTIVLDELDAEVAETFERALDTLARQGALIERIEFPEFLDVGVIGMKGGFAAAESYAWHRFLLTA
KGDVYDPRVSVRILRGEAITVPDYIEMLNARRSLVTRAAARIAPYDALVMPTTANAPPKIADLADDAAFARENIRALRNC
TFINMIDGCAISLPAHRHGEVPVGLMLAQSGGNDRKLLEIAAGIEGVVRG

Sequences:

>Translated_450_residues
MTAANPTLAALADDLAAGRTTSRKLVEDCLVRIADPAGEGQRAFIHVDKDAALSAADGMDALRKANAAPSAFAGIPVSIK
DLFDIKGQVTRAGSRALEDSAPAEADAPAVARLRKAGFVVIGRTNMTEFAYSGIGINPHYGTPKSAWKREVGYVPGGSSS
GAAVSVADRMAYGALGTDTGGSCRIPAAFNGITGYKPTQARVPLDGGVPLSSTLDSFGPLANTVACCAVLDSVLADEPIR
PLASRPVKGLRLAVPTTIVLDELDAEVAETFERALDTLARQGALIERIEFPEFLDVGVIGMKGGFAAAESYAWHRFLLTA
KGDVYDPRVSVRILRGEAITVPDYIEMLNARRSLVTRAAARIAPYDALVMPTTANAPPKIADLADDAAFARENIRALRNC
TFINMIDGCAISLPAHRHGEVPVGLMLAQSGGNDRKLLEIAAGIEGVVRG
>Mature_449_residues
TAANPTLAALADDLAAGRTTSRKLVEDCLVRIADPAGEGQRAFIHVDKDAALSAADGMDALRKANAAPSAFAGIPVSIKD
LFDIKGQVTRAGSRALEDSAPAEADAPAVARLRKAGFVVIGRTNMTEFAYSGIGINPHYGTPKSAWKREVGYVPGGSSSG
AAVSVADRMAYGALGTDTGGSCRIPAAFNGITGYKPTQARVPLDGGVPLSSTLDSFGPLANTVACCAVLDSVLADEPIRP
LASRPVKGLRLAVPTTIVLDELDAEVAETFERALDTLARQGALIERIEFPEFLDVGVIGMKGGFAAAESYAWHRFLLTAK
GDVYDPRVSVRILRGEAITVPDYIEMLNARRSLVTRAAARIAPYDALVMPTTANAPPKIADLADDAAFARENIRALRNCT
FINMIDGCAISLPAHRHGEVPVGLMLAQSGGNDRKLLEIAAGIEGVVRG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=495, Percent_Identity=25.4545454545455, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI195972892, Length=140, Percent_Identity=35.7142857142857, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI166795287, Length=133, Percent_Identity=35.3383458646617, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=421, Percent_Identity=26.603325415677, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17537465, Length=254, Percent_Identity=26.3779527559055, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=157, Percent_Identity=35.031847133758, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=152, Percent_Identity=34.8684210526316, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17556276, Length=152, Percent_Identity=34.8684210526316, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71990152, Length=193, Percent_Identity=31.6062176165803, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=156, Percent_Identity=32.6923076923077, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=383, Percent_Identity=30.0261096605744, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323950, Length=165, Percent_Identity=34.5454545454545, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648113, Length=491, Percent_Identity=27.6985743380855, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24648435, Length=138, Percent_Identity=34.7826086956522, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648437, Length=138, Percent_Identity=34.7826086956522, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45550774, Length=138, Percent_Identity=34.7826086956522, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648441, Length=138, Percent_Identity=34.7826086956522, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24648439, Length=138, Percent_Identity=34.7826086956522, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24652985, Length=185, Percent_Identity=35.6756756756757, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922090, Length=185, Percent_Identity=35.6756756756757, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24652981, Length=185, Percent_Identity=35.6756756756757, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24652983, Length=185, Percent_Identity=35.6756756756757, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 47147; Mature: 47016

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAANPTLAALADDLAAGRTTSRKLVEDCLVRIADPAGEGQRAFIHVDKDAALSAADGMD
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHCCHH
ALRKANAAPSAFAGIPVSIKDLFDIKGQVTRAGSRALEDSAPAEADAPAVARLRKAGFVV
HHHHCCCCCCHHCCCCEEHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEE
IGRTNMTEFAYSGIGINPHYGTPKSAWKREVGYVPGGSSSGAAVSVADRMAYGALGTDTG
EECCCCHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEHHHHHHHCCCCCCCC
GSCRIPAAFNGITGYKPTQARVPLDGGVPLSSTLDSFGPLANTVACCAVLDSVLADEPIR
CCEECCHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCC
PLASRPVKGLRLAVPTTIVLDELDAEVAETFERALDTLARQGALIERIEFPEFLDVGVIG
HHHHCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHCCCEEE
MKGGFAAAESYAWHRFLLTAKGDVYDPRVSVRILRGEAITVPDYIEMLNARRSLVTRAAA
ECCCCHHHHHHCEEEEEEEECCCCCCCCEEEEEEECCEEECHHHHHHHHHHHHHHHHHHH
RIAPYDALVMPTTANAPPKIADLADDAAFARENIRALRNCTFINMIDGCAISLPAHRHGE
HHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCC
VPVGLMLAQSGGNDRKLLEIAAGIEGVVRG
CCEEEEEECCCCCCHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
TAANPTLAALADDLAAGRTTSRKLVEDCLVRIADPAGEGQRAFIHVDKDAALSAADGMD
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHCCHH
ALRKANAAPSAFAGIPVSIKDLFDIKGQVTRAGSRALEDSAPAEADAPAVARLRKAGFVV
HHHHCCCCCCHHCCCCEEHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEE
IGRTNMTEFAYSGIGINPHYGTPKSAWKREVGYVPGGSSSGAAVSVADRMAYGALGTDTG
EECCCCHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEHHHHHHHCCCCCCCC
GSCRIPAAFNGITGYKPTQARVPLDGGVPLSSTLDSFGPLANTVACCAVLDSVLADEPIR
CCEECCHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCC
PLASRPVKGLRLAVPTTIVLDELDAEVAETFERALDTLARQGALIERIEFPEFLDVGVIG
HHHHCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHCCCEEE
MKGGFAAAESYAWHRFLLTAKGDVYDPRVSVRILRGEAITVPDYIEMLNARRSLVTRAAA
ECCCCHHHHHHCEEEEEEEECCCCCCCCEEEEEEECCEEECHHHHHHHHHHHHHHHHHHH
RIAPYDALVMPTTANAPPKIADLADDAAFARENIRALRNCTFINMIDGCAISLPAHRHGE
HHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCC
VPVGLMLAQSGGNDRKLLEIAAGIEGVVRG
CCEEEEEECCCCCCHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA