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The map label for this gene is leuB [H]

Identifier: 146339470

GI number: 146339470

Start: 2618692

End: 2619771

Strand: Direct

Name: leuB [H]

Synonym: BRADO2458

Alternate gene names: 146339470

Gene position: 2618692-2619771 (Clockwise)

Preceding gene: 146339469

Following gene: 146339477

Centisome position: 35.12

GC content: 66.57

Gene sequence:

>1080_bases
ATGCCCTCCACCAACGCCCTCCACATCGCAGTCCTCCCCGGCGACGGCATCGGTCCTGAAGTGATGGCGCCGGCGCTTGA
AGTATTGCGCAAGGTCGAGGCCAGGGCCGGCCTGTCGTTCCGCTTCACCGAGGCCCAGGCCGGCGCCAACAATTATCTCG
CCACCGGCGTCTCGATCGCCGAGTCGACGATCCGGCTGTGCGAGCAGGCCGATGCCATCCTGCTCGGCGCCTGCGGACTG
CCGTCGGTGCGCTATCCCGACAACACCGAGATCGCGCCGCAGATCGAGCTGCGCTTCCATTTCGATCTCTATGCCGGGGT
GCGGCCGGCGCGGCTGATCCCGGGCGTGCCGAGCCCGATCGTCGGCGCCGCCGAGCGCGGCATCGACTTCGTCGTCATCC
GCGAGTCGACCGAAGGCCTGTTCGCCTCGATGGGCAAAGGTCAGGTGACACATGAAGAGGCGCGCGAGACCCTCGTCATC
ACGCGCAGAACCTCCGAGCGGCTGTTCAACTTCTCCTTCAAGCTCGCCGAACGGCGCCGCGCGCGCGGCCGCTCCGGCAC
GTTGGGCTGTGTCGACAAGGCCAATGTGTTCAAGGCCTTCGCCTACTTTCGCAAGATCTTCGACGAGGTCGCCGAGCTGC
ATCCTGAGGTGAAGACAGAGCGGCTCTACATCGATGCCTGCGCGCTGATGCTGGTGAAGCGGCCTTGGGACTTCGACGTG
CTGGTCACCGAGAACATGTTCGGCGACATCCTGTCCGATCTCGCCGCGGGCCTCGTCGGCGGCATGGGCATGGCGCCCTC
GGCCGACATCGGCGACCGACATGCTGTGTTCCAGCCCTGCCATGGCACCGCGCCCGACATCATGGGGCAGGGCAAGGCCA
ACCCCACGGCGATGATCCTATCGGCGGCGATGATGCTCGATTGGCTGGCTGACACCCACGGCATCGAAGGCGCCGCCGAG
GCGGCGCAGCGGATCGAACAGGCGGTCGATCGGGTCTACGCCGCTGGTCTCAAGCCGTTCGAGTTCGGCGGCCATGCCGG
CACGATCGACGTCACCAAGGCGGTGCTGCAAGAGCTGTGA

Upstream 100 bases:

>100_bases
GAGCGAATCGACGAAGCAATCCAGAGTCTTAACCTTGGCCCTGGATTGCTTCGCTTCGCTCGCAATGACGGCAATAACTT
CTTCGAATTCCAAGTGAGAC

Downstream 100 bases:

>100_bases
ACCGGCGTCGGCCGTTCAGAAGATCTGAGCGGTGACAACAGCATAGGCGACGAAGATCACGCCAAGACCCCAGACCATGC
CGGGAATTTTGCCCCCGCGG

Product: putative 3-isopropylmalate dehydrogenase (leuB)

Products: NA

Alternate protein names: 3-isopropylmalate dehydrogenase; 3-IPM-DH; IMDH; IPMDH; Beta-IPM dehydrogenase; D-malate dehydrogenase [decarboxylating] [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MPSTNALHIAVLPGDGIGPEVMAPALEVLRKVEARAGLSFRFTEAQAGANNYLATGVSIAESTIRLCEQADAILLGACGL
PSVRYPDNTEIAPQIELRFHFDLYAGVRPARLIPGVPSPIVGAAERGIDFVVIRESTEGLFASMGKGQVTHEEARETLVI
TRRTSERLFNFSFKLAERRRARGRSGTLGCVDKANVFKAFAYFRKIFDEVAELHPEVKTERLYIDACALMLVKRPWDFDV
LVTENMFGDILSDLAAGLVGGMGMAPSADIGDRHAVFQPCHGTAPDIMGQGKANPTAMILSAAMMLDWLADTHGIEGAAE
AAQRIEQAVDRVYAAGLKPFEFGGHAGTIDVTKAVLQEL

Sequences:

>Translated_359_residues
MPSTNALHIAVLPGDGIGPEVMAPALEVLRKVEARAGLSFRFTEAQAGANNYLATGVSIAESTIRLCEQADAILLGACGL
PSVRYPDNTEIAPQIELRFHFDLYAGVRPARLIPGVPSPIVGAAERGIDFVVIRESTEGLFASMGKGQVTHEEARETLVI
TRRTSERLFNFSFKLAERRRARGRSGTLGCVDKANVFKAFAYFRKIFDEVAELHPEVKTERLYIDACALMLVKRPWDFDV
LVTENMFGDILSDLAAGLVGGMGMAPSADIGDRHAVFQPCHGTAPDIMGQGKANPTAMILSAAMMLDWLADTHGIEGAAE
AAQRIEQAVDRVYAAGLKPFEFGGHAGTIDVTKAVLQEL
>Mature_358_residues
PSTNALHIAVLPGDGIGPEVMAPALEVLRKVEARAGLSFRFTEAQAGANNYLATGVSIAESTIRLCEQADAILLGACGLP
SVRYPDNTEIAPQIELRFHFDLYAGVRPARLIPGVPSPIVGAAERGIDFVVIRESTEGLFASMGKGQVTHEEARETLVIT
RRTSERLFNFSFKLAERRRARGRSGTLGCVDKANVFKAFAYFRKIFDEVAELHPEVKTERLYIDACALMLVKRPWDFDVL
VTENMFGDILSDLAAGLVGGMGMAPSADIGDRHAVFQPCHGTAPDIMGQGKANPTAMILSAAMMLDWLADTHGIEGAAEA
AQRIEQAVDRVYAAGLKPFEFGGHAGTIDVTKAVLQEL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate, which decarboxylates to 4-methyl-2-oxopentanoate (2-oxoisocaproate). Also catalyzes the oxidative decarboxylation of 3-methylmal

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=359, Percent_Identity=34.8189415041783, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI28178821, Length=359, Percent_Identity=33.1476323119777, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI28178816, Length=359, Percent_Identity=33.4261838440111, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4758582, Length=307, Percent_Identity=32.5732899022801, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI28178838, Length=307, Percent_Identity=32.5732899022801, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI28178819, Length=204, Percent_Identity=36.7647058823529, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI87081683, Length=341, Percent_Identity=38.41642228739, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1788101, Length=363, Percent_Identity=35.8126721763085, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1787381, Length=396, Percent_Identity=27.7777777777778, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71986051, Length=344, Percent_Identity=34.593023255814, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17550882, Length=361, Percent_Identity=31.0249307479224, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI25144293, Length=359, Percent_Identity=30.6406685236769, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17505779, Length=359, Percent_Identity=30.08356545961, Blast_Score=141, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6322097, Length=369, Percent_Identity=35.7723577235772, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6319830, Length=368, Percent_Identity=33.1521739130435, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=364, Percent_Identity=31.3186813186813, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324291, Length=362, Percent_Identity=30.1104972375691, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24643270, Length=305, Percent_Identity=32.4590163934426, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24643268, Length=305, Percent_Identity=32.4590163934426, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24661184, Length=364, Percent_Identity=31.043956043956, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI161078635, Length=359, Percent_Identity=28.133704735376, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI161078637, Length=359, Percent_Identity=28.133704735376, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI161078633, Length=359, Percent_Identity=28.133704735376, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24650122, Length=359, Percent_Identity=28.133704735376, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI161078639, Length=357, Percent_Identity=28.2913165266106, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI281362242, Length=364, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24648872, Length=364, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI20130355, Length=278, Percent_Identity=26.978417266187, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85; =1.1.1.83 [H]

Molecular weight: Translated: 38727; Mature: 38596

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTNALHIAVLPGDGIGPEVMAPALEVLRKVEARAGLSFRFTEAQAGANNYLATGVSIA
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCEEHHHHHHH
ESTIRLCEQADAILLGACGLPSVRYPDNTEIAPQIELRFHFDLYAGVRPARLIPGVPSPI
HHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHCCCCCCCC
VGAAERGIDFVVIRESTEGLFASMGKGQVTHEEARETLVITRRTSERLFNFSFKLAERRR
HHHHHCCCEEEEEECCCCCHHHHCCCCCCCHHHHHCEEEEEECCHHHHHHHHHHHHHHHH
ARGRSGTLGCVDKANVFKAFAYFRKIFDEVAELHPEVKTERLYIDACALMLVKRPWDFDV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEE
LVTENMFGDILSDLAAGLVGGMGMAPSADIGDRHAVFQPCHGTAPDIMGQGKANPTAMIL
EEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHH
SAAMMLDWLADTHGIEGAAEAAQRIEQAVDRVYAAGLKPFEFGGHAGTIDVTKAVLQEL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHC
>Mature Secondary Structure 
PSTNALHIAVLPGDGIGPEVMAPALEVLRKVEARAGLSFRFTEAQAGANNYLATGVSIA
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCEEHHHHHHH
ESTIRLCEQADAILLGACGLPSVRYPDNTEIAPQIELRFHFDLYAGVRPARLIPGVPSPI
HHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHCCCCCCCC
VGAAERGIDFVVIRESTEGLFASMGKGQVTHEEARETLVITRRTSERLFNFSFKLAERRR
HHHHHCCCEEEEEECCCCCHHHHCCCCCCCHHHHHCEEEEEECCHHHHHHHHHHHHHHHH
ARGRSGTLGCVDKANVFKAFAYFRKIFDEVAELHPEVKTERLYIDACALMLVKRPWDFDV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEE
LVTENMFGDILSDLAAGLVGGMGMAPSADIGDRHAVFQPCHGTAPDIMGQGKANPTAMIL
EEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHH
SAAMMLDWLADTHGIEGAAEAAQRIEQAVDRVYAAGLKPFEFGGHAGTIDVTKAVLQEL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087; 10940051 [H]