Click here to switch to the map view.

The map label for this gene is fixI [H]

Identifier: 146339458

GI number: 146339458

Start: 2604145

End: 2606346

Strand: Direct

Name: fixI [H]

Synonym: BRADO2444

Alternate gene names: 146339458

Gene position: 2604145-2606346 (Clockwise)

Preceding gene: 146339457

Following gene: 146339459

Centisome position: 34.92

GC content: 68.3

Gene sequence:

>2202_bases
ATGCACGTGACGCGTGACTTCTCCCACTATGTGAAAGATCTCGGCAAGGGCCTCAAGCACATCGACCTCGCGGTCGAGGG
CGTGAGCTGCGCCGGCTGCATGGCCAAGATCGAGCGCGGCTTGTCGGCGCTGCCGGATGTGACCTTGGCGCGCGTCAATC
TCACCGACCGCCGCGTCGCGCTGGAGTGGAAGCAGGGCACGCTCGATCCGGCAAAATTCATCGATCGCCTCGCCGAGCTC
GGCTACAAGGCCTATCCGTTCGAGAAGGCGAGCGCAGAGGCCACCGAAGCCGAGGAATCGCGCTTCCTCCTGCGCTGCCT
CGGCGTCGCGGCGTTCGCGACCATGAACGTGATGATGCTGTCGATCCCGGTCTGGTCCGGCAATGTCAGCGACATGCTGC
CGGAGCAGCGCGACTTCTTCCACTGGCTGTCGGCCGTGATCGCGCTGCCGGCCGCGGCCTATGCCGGCCAGCCGTTCTTC
CGCTCGGCGTGGCGCGCCTTGAAGACCGGTAACGTCAACATGGACGTGCCGATCTGCATCGGCGTGATCCTGGCCTTGGG
CATGTCGATCGTCGAGACGTTCCATCACGCCGAGCACGCTTATTTCGATGCCGCGATCATGCTGCTGACCTTCCTCCTGG
TCGGCCGCTATCTCGATCAGAACATGCGGCGGCGGACCCGGGCGGTCGCCGGCAATCTCGCCGCGCTGAAGGCGGAGACC
GCGACCAAGTTCATCGGCCCGGATGAAATCTCCGAGGTGCCCGTGGCCGCCGTCCGCGCCGGCGATCTGGTGCTGCTGCG
CCCCGGCGAGCGTTGCGCCGTCGACGGCGTCGTGATCGAGGGCCGCTCCGAGATCGACCAGAGCCTGATCACCGGCGAGA
CGCTGTATGTGCCGGCCGAGCAGGGCACCGCGGTCTATGCCGGCTCGCTGAACATTTCCGGCACGCTGCGCGTCCGCGTC
TCGGCCGCCGCCGAGGGCACGCTGCTCGCCGAGATCACGCGCCTGCTCGACACAGCGCTGCAGGCGCGCTCGCGCTACAT
GCGGCTCGCCGATCGCGCCTCGCGGCTGTATGCGCCGGTGGTGCACGCCACCGCCGCGCTGACGGTGCTGGGCTGGGTGC
TGGCCGGCGCGAGCTGGCACGATGCCATCGTCACCGGCATCGCCGTGCTGATCATCACCTGTCCCTGCGCGCTCGGCCTT
GCCATTCCCACCGTGCAGACGGTCGCCTCCGGCGCGATGTTCAAGTCAGGCGTGCTGCTCAATGCGGGCGACGCGATCGA
GCGGCTCGCCGAGGCCGATCACGTGATCTTCGATAAGACCGGCACCTTGACCTTGCCGGAGCTCGAAGTGGTCAACGCCG
CCGACATTCCGGCGGATGTCTTCTCCGTCGCCGGCCAGCTCGCGCTGTCGAGCCATCACCCCGTGGCGGCGGCGGTCGCG
CAGGCCGCGAAGTCGCGGTCGCCGCTGATGGGCGCGGTCGAGGAGCCCGGGCAGGGCGTGCGCGCGGTGGTCGATGGCGT
CGAGCTCCGGCTTGGCCGTCCCTCCTTCTGCGACGCCGAGGCGCTGGTGGCGCAGAGCGCCAGCGTCGATCCCGAAGCGT
CCGTCGTGGCCTTCTGCCGTGGCGATCAAAAATATCTGTTCTCCGTGCGCCAGGGTTTGCGTCCCGATGCCGCGAAGATC
GTCGCCGGCCTGCAGCGCCGCGGCATCAAGGTCGAAATCCTCTCGGGCGATCGCGAGGCGGCCGTCGCCGCCGCCGCGAA
GGCGCTCGGGATCAGCGAATGGCGCGCCGGCGTCACCCCGGCCGACAAGATCGCTCGCGTCGAAGAGCTGAAGCGCGCCA
ACGTCAAGGTCATGATGGTCGGCGACGGCATGAACGACGCGCCGTCGCTCGCTGCCGCGCATGTCTCGATGTCGCCGATC
TCGGCTGCGCATCTCAGCCAGGCTACCGCCGATCTCGTCTTCCTCGGCCGTCCCTTGGCGCCGGTGCTGGCCGCGATCGA
CTATGCGCGCAAGGCGCTGCATCTGATGCGGCAAAATCTCTGGCTCGCGGTGCTCTACAATCTGTTCGCCGTGCCGCTCG
CCATCAGCGGCGTCGTCACGCCCTTGATCGCGGCCGCCGCGATGTCGGGCTCGTCGATCCTGGTGATGCTGAATGCGCTG
CGCGCCCGCAAGGTCCGCGAGGAGGCCGCGTCATGGAAGTGA

Upstream 100 bases:

>100_bases
CGCCGGGCCAGTGGGATCTCGTGCTCGAAGGTGATTCCAGCGGTCGGCGGCTGTTCCTGTCGAAGAACCGCGTGCTGCTG
AACTGAAGGACTGACTGATC

Downstream 100 bases:

>100_bases
TGATCATTCTCGTCCCGCTCGCACTCGCACTGGGCTTGGCCGGCCTGGTCGGCTTCCTCTGGTCGCTGAAGAGCGGCCAG
TACGAGGATCTCGAAGGCGC

Product: nitrogen fixation protein fixI

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 733; Mature: 733

Protein sequence:

>733_residues
MHVTRDFSHYVKDLGKGLKHIDLAVEGVSCAGCMAKIERGLSALPDVTLARVNLTDRRVALEWKQGTLDPAKFIDRLAEL
GYKAYPFEKASAEATEAEESRFLLRCLGVAAFATMNVMMLSIPVWSGNVSDMLPEQRDFFHWLSAVIALPAAAYAGQPFF
RSAWRALKTGNVNMDVPICIGVILALGMSIVETFHHAEHAYFDAAIMLLTFLLVGRYLDQNMRRRTRAVAGNLAALKAET
ATKFIGPDEISEVPVAAVRAGDLVLLRPGERCAVDGVVIEGRSEIDQSLITGETLYVPAEQGTAVYAGSLNISGTLRVRV
SAAAEGTLLAEITRLLDTALQARSRYMRLADRASRLYAPVVHATAALTVLGWVLAGASWHDAIVTGIAVLIITCPCALGL
AIPTVQTVASGAMFKSGVLLNAGDAIERLAEADHVIFDKTGTLTLPELEVVNAADIPADVFSVAGQLALSSHHPVAAAVA
QAAKSRSPLMGAVEEPGQGVRAVVDGVELRLGRPSFCDAEALVAQSASVDPEASVVAFCRGDQKYLFSVRQGLRPDAAKI
VAGLQRRGIKVEILSGDREAAVAAAAKALGISEWRAGVTPADKIARVEELKRANVKVMMVGDGMNDAPSLAAAHVSMSPI
SAAHLSQATADLVFLGRPLAPVLAAIDYARKALHLMRQNLWLAVLYNLFAVPLAISGVVTPLIAAAAMSGSSILVMLNAL
RARKVREEAASWK

Sequences:

>Translated_733_residues
MHVTRDFSHYVKDLGKGLKHIDLAVEGVSCAGCMAKIERGLSALPDVTLARVNLTDRRVALEWKQGTLDPAKFIDRLAEL
GYKAYPFEKASAEATEAEESRFLLRCLGVAAFATMNVMMLSIPVWSGNVSDMLPEQRDFFHWLSAVIALPAAAYAGQPFF
RSAWRALKTGNVNMDVPICIGVILALGMSIVETFHHAEHAYFDAAIMLLTFLLVGRYLDQNMRRRTRAVAGNLAALKAET
ATKFIGPDEISEVPVAAVRAGDLVLLRPGERCAVDGVVIEGRSEIDQSLITGETLYVPAEQGTAVYAGSLNISGTLRVRV
SAAAEGTLLAEITRLLDTALQARSRYMRLADRASRLYAPVVHATAALTVLGWVLAGASWHDAIVTGIAVLIITCPCALGL
AIPTVQTVASGAMFKSGVLLNAGDAIERLAEADHVIFDKTGTLTLPELEVVNAADIPADVFSVAGQLALSSHHPVAAAVA
QAAKSRSPLMGAVEEPGQGVRAVVDGVELRLGRPSFCDAEALVAQSASVDPEASVVAFCRGDQKYLFSVRQGLRPDAAKI
VAGLQRRGIKVEILSGDREAAVAAAAKALGISEWRAGVTPADKIARVEELKRANVKVMMVGDGMNDAPSLAAAHVSMSPI
SAAHLSQATADLVFLGRPLAPVLAAIDYARKALHLMRQNLWLAVLYNLFAVPLAISGVVTPLIAAAAMSGSSILVMLNAL
RARKVREEAASWK
>Mature_733_residues
MHVTRDFSHYVKDLGKGLKHIDLAVEGVSCAGCMAKIERGLSALPDVTLARVNLTDRRVALEWKQGTLDPAKFIDRLAEL
GYKAYPFEKASAEATEAEESRFLLRCLGVAAFATMNVMMLSIPVWSGNVSDMLPEQRDFFHWLSAVIALPAAAYAGQPFF
RSAWRALKTGNVNMDVPICIGVILALGMSIVETFHHAEHAYFDAAIMLLTFLLVGRYLDQNMRRRTRAVAGNLAALKAET
ATKFIGPDEISEVPVAAVRAGDLVLLRPGERCAVDGVVIEGRSEIDQSLITGETLYVPAEQGTAVYAGSLNISGTLRVRV
SAAAEGTLLAEITRLLDTALQARSRYMRLADRASRLYAPVVHATAALTVLGWVLAGASWHDAIVTGIAVLIITCPCALGL
AIPTVQTVASGAMFKSGVLLNAGDAIERLAEADHVIFDKTGTLTLPELEVVNAADIPADVFSVAGQLALSSHHPVAAAVA
QAAKSRSPLMGAVEEPGQGVRAVVDGVELRLGRPSFCDAEALVAQSASVDPEASVVAFCRGDQKYLFSVRQGLRPDAAKI
VAGLQRRGIKVEILSGDREAAVAAAAKALGISEWRAGVTPADKIARVEELKRANVKVMMVGDGMNDAPSLAAAHVSMSPI
SAAHLSQATADLVFLGRPLAPVLAAIDYARKALHLMRQNLWLAVLYNLFAVPLAISGVVTPLIAAAAMSGSSILVMLNAL
RARKVREEAASWK

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=816, Percent_Identity=27.5735294117647, Blast_Score=271, Evalue=2e-72,
Organism=Homo sapiens, GI115529486, Length=825, Percent_Identity=25.8181818181818, Blast_Score=257, Evalue=3e-68,
Organism=Homo sapiens, GI55743073, Length=545, Percent_Identity=29.3577981651376, Blast_Score=196, Evalue=5e-50,
Organism=Escherichia coli, GI1786691, Length=735, Percent_Identity=30.7482993197279, Blast_Score=274, Evalue=2e-74,
Organism=Escherichia coli, GI1789879, Length=715, Percent_Identity=28.8111888111888, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1786914, Length=498, Percent_Identity=26.9076305220884, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17556548, Length=804, Percent_Identity=25.6218905472637, Blast_Score=196, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6320475, Length=748, Percent_Identity=25.668449197861, Blast_Score=229, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6319772, Length=629, Percent_Identity=29.093799682035, Blast_Score=227, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6321430, Length=537, Percent_Identity=23.463687150838, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6325221, Length=558, Percent_Identity=23.4767025089606, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI221329854, Length=582, Percent_Identity=28.3505154639175, Blast_Score=192, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 77935; Mature: 77935

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS00154 ATPASE_E1_E2 ; PS01047 HMA_1 ; PS50846 HMA_2 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVTRDFSHYVKDLGKGLKHIDLAVEGVSCAGCMAKIERGLSALPDVTLARVNLTDRRVA
CCCCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEE
LEWKQGTLDPAKFIDRLAELGYKAYPFEKASAEATEAEESRFLLRCLGVAAFATMNVMML
EEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEE
SIPVWSGNVSDMLPEQRDFFHWLSAVIALPAAAYAGQPFFRSAWRALKTGNVNMDVPICI
EEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHH
GVILALGMSIVETFHHAEHAYFDAAIMLLTFLLVGRYLDQNMRRRTRAVAGNLAALKAET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ATKFIGPDEISEVPVAAVRAGDLVLLRPGERCAVDGVVIEGRSEIDQSLITGETLYVPAE
HHHCCCCCHHHHCCCHHEECCCEEEECCCCCEEECCEEECCHHHHHHHHHCCCEEEEECC
QGTAVYAGSLNISGTLRVRVSAAAEGTLLAEITRLLDTALQARSRYMRLADRASRLYAPV
CCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHATAALTVLGWVLAGASWHDAIVTGIAVLIITCPCALGLAIPTVQTVASGAMFKSGVLL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEE
NAGDAIERLAEADHVIFDKTGTLTLPELEVVNAADIPADVFSVAGQLALSSHHPVAAAVA
ECCHHHHHHHHCCCEEEECCCCEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
QAAKSRSPLMGAVEEPGQGVRAVVDGVELRLGRPSFCDAEALVAQSASVDPEASVVAFCR
HHHHCCCCCCCCCCCCCCCHHHHHCCHHEECCCCCCCCHHHHHHHCCCCCCCCCEEEEEC
GDQKYLFSVRQGLRPDAAKIVAGLQRRGIKVEILSGDREAAVAAAAKALGISEWRAGVTP
CCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCHHHHHCCCCC
ADKIARVEELKRANVKVMMVGDGMNDAPSLAAAHVSMSPISAAHLSQATADLVFLGRPLA
HHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHEECCCHH
PVLAAIDYARKALHLMRQNLWLAVLYNLFAVPLAISGVVTPLIAAAAMSGSSILVMLNAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RARKVREEAASWK
HHHHHHHHHCCCC
>Mature Secondary Structure
MHVTRDFSHYVKDLGKGLKHIDLAVEGVSCAGCMAKIERGLSALPDVTLARVNLTDRRVA
CCCCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEE
LEWKQGTLDPAKFIDRLAELGYKAYPFEKASAEATEAEESRFLLRCLGVAAFATMNVMML
EEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEE
SIPVWSGNVSDMLPEQRDFFHWLSAVIALPAAAYAGQPFFRSAWRALKTGNVNMDVPICI
EEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHH
GVILALGMSIVETFHHAEHAYFDAAIMLLTFLLVGRYLDQNMRRRTRAVAGNLAALKAET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ATKFIGPDEISEVPVAAVRAGDLVLLRPGERCAVDGVVIEGRSEIDQSLITGETLYVPAE
HHHCCCCCHHHHCCCHHEECCCEEEECCCCCEEECCEEECCHHHHHHHHHCCCEEEEECC
QGTAVYAGSLNISGTLRVRVSAAAEGTLLAEITRLLDTALQARSRYMRLADRASRLYAPV
CCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHATAALTVLGWVLAGASWHDAIVTGIAVLIITCPCALGLAIPTVQTVASGAMFKSGVLL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEE
NAGDAIERLAEADHVIFDKTGTLTLPELEVVNAADIPADVFSVAGQLALSSHHPVAAAVA
ECCHHHHHHHHCCCEEEECCCCEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
QAAKSRSPLMGAVEEPGQGVRAVVDGVELRLGRPSFCDAEALVAQSASVDPEASVVAFCR
HHHHCCCCCCCCCCCCCCCHHHHHCCHHEECCCCCCCCHHHHHHHCCCCCCCCCEEEEEC
GDQKYLFSVRQGLRPDAAKIVAGLQRRGIKVEILSGDREAAVAAAAKALGISEWRAGVTP
CCHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCHHHHHCCCCC
ADKIARVEELKRANVKVMMVGDGMNDAPSLAAAHVSMSPISAAHLSQATADLVFLGRPLA
HHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHEECCCHH
PVLAAIDYARKALHLMRQNLWLAVLYNLFAVPLAISGVVTPLIAAAAMSGSSILVMLNAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RARKVREEAASWK
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8661920; 12597275 [H]