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The map label for this gene is rutB [H]

Identifier: 146339416

GI number: 146339416

Start: 2560481

End: 2561167

Strand: Direct

Name: rutB [H]

Synonym: BRADO2402

Alternate gene names: 146339416

Gene position: 2560481-2561167 (Clockwise)

Preceding gene: 146339415

Following gene: 146339417

Centisome position: 34.34

GC content: 66.81

Gene sequence:

>687_bases
ATGGCGAACCTAGCTGCATCGGCAACCGCAACGATCATGGCCGAACCGGAGCCGATCGCGCTCGATCTGTCGAAGACGGC
GCTGGTCATCATCGACATGCAGCGCGACTTCATGGAGCCCGGCGGCTTTGGCGAGACGCTGGGCAATGACGTGTCGCAGC
TCGCGCGTGCCGTGCAGCCGATCGCAAACGTGCTCGCCGCGGCGCGCAAGGCCGGCATGCTGGTCATCCATACCCGCGAG
GGGCACAAGCCTGATCTCTCCGACGCGCCGCCGGCCAAGGTCGAGCGCGGCGCGCCGTCTTTGCGCATCGGCGATCCCGG
CCCGATGGGCCGCATCCTCATTCGCGGCGAGGCCGGGCACGACATCATTCCCGCGCTCTATCCCACGGACGGCGAAGTGG
TGATCGACAAGCCGGGGAAGGGCGCGTTTTACGCCACCGAGATGGGCGACGTGCTGAAGCAGCACGGCATCGAGAACCTG
CTGGTGTGCGGCGTCACCACCGAGGTCTGCGTCAACACCACCGTGCGCGAGGCCAATGACCGCGGCTATCGCTGCGTGGT
CGTCTCCGACGGCTGCGCGTCCTACTTCCCGGAGTTCCACGAGATGGGCCTGAAGATGATCAAGGCCCAGGGCGGCATCT
TCGGCTGGGTCGCGGAGTCAGCCGCGGTGCTGAAGGCCATGGTCTGA

Upstream 100 bases:

>100_bases
GTGCTTCGCGGCCGCCGGCGCTGTCTGCCCAATTGTACACAATGGCACACCGCTTGCTTCGTTGTCTGCGACGCATTCTC
GCGGGGAGGCACGCGGCGAC

Downstream 100 bases:

>100_bases
GAACCGGCCTGAGAGACGGACTGAGAGATCTGAGACGGATGAGTTCGATCATGTAGTCGCTGACGGCAAACAGGGGAGCA
AGGCGATGAGCGGAAGCGGG

Product: putative isochorismatase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MANLAASATATIMAEPEPIALDLSKTALVIIDMQRDFMEPGGFGETLGNDVSQLARAVQPIANVLAAARKAGMLVIHTRE
GHKPDLSDAPPAKVERGAPSLRIGDPGPMGRILIRGEAGHDIIPALYPTDGEVVIDKPGKGAFYATEMGDVLKQHGIENL
LVCGVTTEVCVNTTVREANDRGYRCVVVSDGCASYFPEFHEMGLKMIKAQGGIFGWVAESAAVLKAMV

Sequences:

>Translated_228_residues
MANLAASATATIMAEPEPIALDLSKTALVIIDMQRDFMEPGGFGETLGNDVSQLARAVQPIANVLAAARKAGMLVIHTRE
GHKPDLSDAPPAKVERGAPSLRIGDPGPMGRILIRGEAGHDIIPALYPTDGEVVIDKPGKGAFYATEMGDVLKQHGIENL
LVCGVTTEVCVNTTVREANDRGYRCVVVSDGCASYFPEFHEMGLKMIKAQGGIFGWVAESAAVLKAMV
>Mature_227_residues
ANLAASATATIMAEPEPIALDLSKTALVIIDMQRDFMEPGGFGETLGNDVSQLARAVQPIANVLAAARKAGMLVIHTREG
HKPDLSDAPPAKVERGAPSLRIGDPGPMGRILIRGEAGHDIIPALYPTDGEVVIDKPGKGAFYATEMGDVLKQHGIENLL
VCGVTTEVCVNTTVREANDRGYRCVVVSDGCASYFPEFHEMGLKMIKAQGGIFGWVAESAAVLKAMV

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=229, Percent_Identity=29.6943231441048, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI87081992, Length=189, Percent_Identity=26.984126984127, Blast_Score=71, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 24111; Mature: 23980

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLAASATATIMAEPEPIALDLSKTALVIIDMQRDFMEPGGFGETLGNDVSQLARAVQP
CCCCCCCCEEEEEECCCCEEEEECCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHH
IANVLAAARKAGMLVIHTREGHKPDLSDAPPAKVERGAPSLRIGDPGPMGRILIRGEAGH
HHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHCCCCCEEECCCCCCCEEEEECCCCC
DIIPALYPTDGEVVIDKPGKGAFYATEMGDVLKQHGIENLLVCGVTTEVCVNTTVREAND
CCCCEEECCCCCEEEECCCCCCEEEHHHHHHHHHCCCCEEEEECCCHHHHHCCHHHCCCC
RGYRCVVVSDGCASYFPEFHEMGLKMIKAQGGIFGWVAESAAVLKAMV
CCEEEEEEECCHHHHCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCC
>Mature Secondary Structure 
ANLAASATATIMAEPEPIALDLSKTALVIIDMQRDFMEPGGFGETLGNDVSQLARAVQP
CCCCCCCEEEEEECCCCEEEEECCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHH
IANVLAAARKAGMLVIHTREGHKPDLSDAPPAKVERGAPSLRIGDPGPMGRILIRGEAGH
HHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHCCCCCEEECCCCCCCEEEEECCCCC
DIIPALYPTDGEVVIDKPGKGAFYATEMGDVLKQHGIENLLVCGVTTEVCVNTTVREAND
CCCCEEECCCCCEEEECCCCCCEEEHHHHHHHHHCCCCEEEEECCCHHHHHCCHHHCCCC
RGYRCVVVSDGCASYFPEFHEMGLKMIKAQGGIFGWVAESAAVLKAMV
CCEEEEEEECCHHHHCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA