BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is livF [H]

Identifier: 146339292

GI number: 146339292

Start: 2416760

End: 2417467

Strand: Direct

Name: livF [H]

Synonym: BRADO2268

Alternate gene names: 146339292

Gene position: 2416760-2417467 (Clockwise)

Preceding gene: 146339291

Following gene: 146339293

Centisome position: 32.41

GC content: 67.23

Gene sequence:

>708_bases
ATGGCGCTGCTCGAAATTCAGGGGCTGACCGCCTTCTACCGCGACTTCCAGGCGCTGTTCGGCGTCGACCTCATGCTTGA
GGCGGGCGAGACGATTGCGATCGTCGGCGCCAACGGCGCCGGCAAGTCGACTTTGATGCGCTCGATCTCCGGCGTGCTGC
GCAACGGCGCCGATCAGATCGTGTTCGACGGACGTCCGATCGGCGCGCTCGCTGCGCCTGACGTCGTGGCGCTCGGCGTC
GCGATGGTGCCGGAGGGACGAAAACTGTTTCCCTCGCTGTCGGTGGAAGAGAACCTGCTGATCGGACGCTACGGCCGCGC
GACGCCGGGACCGTGGTCGCTCGAGACGATCTACCGGCTGTTCCCGGTGCTGAAGGAGCGGCGCCACACCCCGGGCACCG
CGCTGTCCGGCGGCCAGCAGCAGATGGTGGCGATCGGACGGGCACTGATGTCGAACCCGCGCGTGCTGCTGTGCGATGAG
CTGAGCCTCGGCCTCGCGCCGATCATCATCAAGGATATCTATGCGGCGTTCCCGGAGATCCGCGCCAGCGGCGCCTCGAT
CGTCATCGTCGAGCAGGACATCGGCCAGGCGATGAAGGTCGCCGATCGGCTGTACTGCATGATGGAGGGCCGCGTGACCC
TGAGCGGCCGGCCTGCCGAGCTCAGCCGCGAGGCGATCCACGCGGCGTATTTCGGAGCACACGCATGA

Upstream 100 bases:

>100_bases
TACTCGTGCTCAATTTCGGCAAGGTCATCGGCATCGGCAAGCCCGAGGAGATCATGGCCTCGAGCGCGGTGCGCGAGATC
TATCTGGGGATCGAGGTCTG

Downstream 100 bases:

>100_bases
GCTGGGCAGACACCATCCTGCAGGGCGTGCTGCTCGGCGGCCTCTACGCGTTGTTCGCTGCAGGTCTTTCGCTGGTGTTC
GGCATCATGCGGCTGGTGAA

Product: high-affinity branched-chain amino acid ABC transporter ATP-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein F [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MALLEIQGLTAFYRDFQALFGVDLMLEAGETIAIVGANGAGKSTLMRSISGVLRNGADQIVFDGRPIGALAAPDVVALGV
AMVPEGRKLFPSLSVEENLLIGRYGRATPGPWSLETIYRLFPVLKERRHTPGTALSGGQQQMVAIGRALMSNPRVLLCDE
LSLGLAPIIIKDIYAAFPEIRASGASIVIVEQDIGQAMKVADRLYCMMEGRVTLSGRPAELSREAIHAAYFGAHA

Sequences:

>Translated_235_residues
MALLEIQGLTAFYRDFQALFGVDLMLEAGETIAIVGANGAGKSTLMRSISGVLRNGADQIVFDGRPIGALAAPDVVALGV
AMVPEGRKLFPSLSVEENLLIGRYGRATPGPWSLETIYRLFPVLKERRHTPGTALSGGQQQMVAIGRALMSNPRVLLCDE
LSLGLAPIIIKDIYAAFPEIRASGASIVIVEQDIGQAMKVADRLYCMMEGRVTLSGRPAELSREAIHAAYFGAHA
>Mature_234_residues
ALLEIQGLTAFYRDFQALFGVDLMLEAGETIAIVGANGAGKSTLMRSISGVLRNGADQIVFDGRPIGALAAPDVVALGVA
MVPEGRKLFPSLSVEENLLIGRYGRATPGPWSLETIYRLFPVLKERRHTPGTALSGGQQQMVAIGRALMSNPRVLLCDEL
SLGLAPIIIKDIYAAFPEIRASGASIVIVEQDIGQAMKVADRLYCMMEGRVTLSGRPAELSREAIHAAYFGAHA

Specific function: Component of the leucine-specific transport system [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI148612844, Length=233, Percent_Identity=30.4721030042918, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI255708477, Length=233, Percent_Identity=30.4721030042918, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI4505771, Length=227, Percent_Identity=29.0748898678414, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI9961250, Length=227, Percent_Identity=29.0748898678414, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI9961252, Length=227, Percent_Identity=29.0748898678414, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI42741659, Length=216, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI27881501, Length=231, Percent_Identity=25.974025974026, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI30795238, Length=231, Percent_Identity=25.974025974026, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI27477115, Length=202, Percent_Identity=26.2376237623762, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI105990541, Length=199, Percent_Identity=26.1306532663317, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI31657092, Length=220, Percent_Identity=25, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI9955963, Length=237, Percent_Identity=27.8481012658228, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI45446740, Length=221, Percent_Identity=25.3393665158371, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI47078218, Length=221, Percent_Identity=25.3393665158371, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI153792144, Length=210, Percent_Identity=25.2380952380952, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI150417984, Length=227, Percent_Identity=25.1101321585903, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI87082268, Length=232, Percent_Identity=44.3965517241379, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1787792, Length=216, Percent_Identity=32.8703703703704, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787029, Length=220, Percent_Identity=32.2727272727273, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1789672, Length=224, Percent_Identity=29.9107142857143, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1787089, Length=225, Percent_Identity=33.7777777777778, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789593, Length=241, Percent_Identity=28.2157676348548, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1790190, Length=223, Percent_Identity=30.0448430493274, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787370, Length=227, Percent_Identity=29.9559471365639, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1789991, Length=243, Percent_Identity=27.1604938271605, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI48995001, Length=221, Percent_Identity=30.7692307692308, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1790544, Length=252, Percent_Identity=29.3650793650794, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1786398, Length=219, Percent_Identity=28.310502283105, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI87081791, Length=222, Percent_Identity=30.6306306306306, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788897, Length=220, Percent_Identity=31.3636363636364, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1789864, Length=249, Percent_Identity=24.8995983935743, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788472, Length=238, Percent_Identity=26.890756302521, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1786872, Length=217, Percent_Identity=30.4147465437788, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788225, Length=233, Percent_Identity=29.6137339055794, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081834, Length=232, Percent_Identity=30.6034482758621, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789032, Length=228, Percent_Identity=28.5087719298246, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790162, Length=225, Percent_Identity=30.2222222222222, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1788450, Length=222, Percent_Identity=28.8288288288288, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787712, Length=226, Percent_Identity=28.7610619469027, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790467, Length=227, Percent_Identity=30.8370044052863, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790739, Length=244, Percent_Identity=28.6885245901639, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1790525, Length=244, Percent_Identity=26.6393442622951, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788210, Length=203, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788761, Length=228, Percent_Identity=27.6315789473684, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1787164, Length=217, Percent_Identity=29.4930875576037, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI87081709, Length=230, Percent_Identity=26.5217391304348, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082267, Length=229, Percent_Identity=31.0043668122271, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081782, Length=229, Percent_Identity=30.1310043668122, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789873, Length=205, Percent_Identity=29.7560975609756, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI48994997, Length=231, Percent_Identity=30.7359307359307, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1787105, Length=226, Percent_Identity=28.7610619469027, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1786703, Length=208, Percent_Identity=30.2884615384615, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1786253, Length=237, Percent_Identity=27.8481012658228, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786563, Length=213, Percent_Identity=29.1079812206573, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787143, Length=244, Percent_Identity=27.4590163934426, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788165, Length=237, Percent_Identity=25.7383966244726, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788644, Length=234, Percent_Identity=29.0598290598291, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786319, Length=199, Percent_Identity=28.1407035175879, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786803, Length=233, Percent_Identity=27.0386266094421, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787112, Length=215, Percent_Identity=28.8372093023256, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI48994883, Length=250, Percent_Identity=25.2, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1786654, Length=193, Percent_Identity=27.979274611399, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1788540, Length=236, Percent_Identity=27.9661016949153, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI193209708, Length=232, Percent_Identity=31.0344827586207, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71984940, Length=227, Percent_Identity=29.9559471365639, Blast_Score=80, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17558664, Length=233, Percent_Identity=29.6137339055794, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193202349, Length=223, Percent_Identity=28.2511210762332, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17543740, Length=152, Percent_Identity=32.8947368421053, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17541710, Length=230, Percent_Identity=28.695652173913, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193211017, Length=218, Percent_Identity=28.4403669724771, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71996809, Length=218, Percent_Identity=28.4403669724771, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193211015, Length=218, Percent_Identity=28.4403669724771, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115533608, Length=210, Percent_Identity=26.1904761904762, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17510237, Length=220, Percent_Identity=25, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71983510, Length=210, Percent_Identity=24.7619047619048, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71983516, Length=210, Percent_Identity=24.7619047619048, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71983523, Length=210, Percent_Identity=24.7619047619048, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323959, Length=219, Percent_Identity=25.1141552511416, Blast_Score=62, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24641565, Length=217, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI221512771, Length=216, Percent_Identity=23.6111111111111, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920532, Length=212, Percent_Identity=27.8301886792453, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24580930, Length=212, Percent_Identity=27.8301886792453, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653245, Length=217, Percent_Identity=29.4930875576037, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281363262, Length=217, Percent_Identity=29.4930875576037, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI85726357, Length=226, Percent_Identity=28.3185840707965, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI85816269, Length=211, Percent_Identity=28.436018957346, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24580553, Length=211, Percent_Identity=28.436018957346, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI85724864, Length=213, Percent_Identity=28.6384976525822, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24580555, Length=213, Percent_Identity=28.6384976525822, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574744, Length=205, Percent_Identity=28.780487804878, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17136592, Length=209, Percent_Identity=25.3588516746411, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17136662, Length=219, Percent_Identity=26.4840182648402, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI161076684, Length=199, Percent_Identity=28.643216080402, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24581617, Length=199, Percent_Identity=28.643216080402, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24581615, Length=199, Percent_Identity=28.643216080402, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 25124; Mature: 24992

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLEIQGLTAFYRDFQALFGVDLMLEAGETIAIVGANGAGKSTLMRSISGVLRNGADQI
CCEEEECHHHHHHHHHHHHHCHHHEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEE
VFDGRPIGALAAPDVVALGVAMVPEGRKLFPSLSVEENLLIGRYGRATPGPWSLETIYRL
EECCCCCCCCCCCHHHHHHHEECCCCCHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHH
FPVLKERRHTPGTALSGGQQQMVAIGRALMSNPRVLLCDELSLGLAPIIIKDIYAAFPEI
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
RASGASIVIVEQDIGQAMKVADRLYCMMEGRVTLSGRPAELSREAIHAAYFGAHA
HCCCCEEEEEEHHHHHHHHHHHHHHHEECCEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALLEIQGLTAFYRDFQALFGVDLMLEAGETIAIVGANGAGKSTLMRSISGVLRNGADQI
CEEEECHHHHHHHHHHHHHCHHHEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEE
VFDGRPIGALAAPDVVALGVAMVPEGRKLFPSLSVEENLLIGRYGRATPGPWSLETIYRL
EECCCCCCCCCCCHHHHHHHEECCCCCHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHHH
FPVLKERRHTPGTALSGGQQQMVAIGRALMSNPRVLLCDELSLGLAPIIIKDIYAAFPEI
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
RASGASIVIVEQDIGQAMKVADRLYCMMEGRVTLSGRPAELSREAIHAAYFGAHA
HCCCCEEEEEEHHHHHHHHHHHHHHHEECCEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]