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The map label for this gene is glyS [H]

Identifier: 146339077

GI number: 146339077

Start: 2159654

End: 2161972

Strand: Direct

Name: glyS [H]

Synonym: BRADO2031

Alternate gene names: 146339077

Gene position: 2159654-2161972 (Clockwise)

Preceding gene: 146339076

Following gene: 146339079

Centisome position: 28.96

GC content: 65.93

Gene sequence:

>2319_bases
ATGCCCGATCTCCTTCTCGAACTGTTCTCCGAAGAAATCCCCGCGCGCATGCAGGCCAAGGCGGCCGACGATCTGCGCCG
CATGGTGACCGACAAGCTCGTCGCCGAGGGCCTGGTCTACGAGGGCGCCAAGGCATTTGCGACGCCGCGCCGGCTGGCGC
TGACTGTGCACGGCATCCCGGCGCGGCAGGCGGATCTGAAGGAAGAGCGCAAGGGGCCGCGCGTCGGCGGACCCGATGCG
GCGATCCAGGGGTTTCTCAAGGCCACGGGTCTCGCCTCGCTCGAAGAGGCGACGATCCAGCGCGATCCGAAGAAGGGCGA
TTTCTACGTCGCGCTGATCGAGAAGCCCGGCCGCGCCACGCTCGACGTGCTCGCCGAGATGCTGCCGGTGATCGTGCGCA
CCTTCCCGTGGCCGAAGTCGATGCGCTGGGGCCAGCGCTCGGAGAAGCCGGGCGCGCTGAGCTGGGTGCGGCCGCTGCAC
GCGATCACCGCGACTTTCGGGCTGGAGACCGAGGAGCCCGATGTCATAAGCTTCGCCGTCGACGGCATCGAGGCCGGGCA
GACCACCTATGGCCACCGCTTCCTGGCGCCATCGGCGTTCACCGTGCGCCGCTTCGAGGACTACGAAGCAAAACTGCTCG
ACGCCAAGGTCGTGCTCGACCCGCAGCGCCGCAAGGACACCATCGTCACCGACGCCAAGCAGCTCGCCTTCGCGCAGGGC
TATGAGCTGGTCGAGGATCCCGTCCTGCTCGACGAGGTCGCGGGCCTGGTCGAATGGCCGGTCGTGCTGATGGGCTCGTT
CGATCCGGAGTATCTGAAGGTCCCGGGCGAGGTGATCCGCGCCACCATCCGCAACAACCAGAAATGCTTCGTGGTCCGCG
ACCCCAAGACGGGCGGGCTGGCGCCAAAGTTCATCCTGACCGCCAACATCGAGGCGACCGACGGCGGCAAGACCATCATC
GCCGGCAACGAGCGCGTCATCCGCGCGCGGCTGTCGGACGCGAAGTTCTTCTACGAGACGGACCTGAAGACCAAGCTCGA
GGACCGGCTGCCGAAGTTCGAGCAGATCGTGTTCCACGAGAAGCTGGGCACGCAGGCCGCGCGCATCGCACGCATCGAGA
AGCTCGCCGCCGAGATCGCGCCGATGGTCGGCGCCGACGCCGCGAAGACCGCGCGCGCGGCGAAGCTTGCGAAAGCCGAT
CTGCTGACGGAGGTCGTCGGCGAGTTTCCGGAAGTACAGGGCCTGATGGGCAAGTACTACGCGCTCGCCCAGGGCGAGGA
CGCCTCGGTCGCCGCCGCGTGCGAGGAGCACTACAAGCCGCAGGGTCCGGGTGATCGCGTGCCGGCCGACCCGGTGAGCG
TGGCCGTGGCACTGGCGGACAAGCTCGATACGCTGGTCGGGTTCTGGGCCATCGACGAGAAGCCGACGGGCTCGAAGGAT
CCGTACGCGCTGAGGCGTGCAGCGCTGGGTGTGATCCGAATCATCTTGGAGAATGATCTCCGCGTTCATCTCGCCGATAT
CTGCGGGCCCGCGTTCTTCGATCTGGCGACGGCAATTCGCGCGCGCGAGGTCGGGAATGAAGTGGAGCAGGCGGAAAGTG
GTGAAAAGCCTGGGTTAACTCGTTTTGTTGTGAGAGTGGATGCGCAAACGTTCGGCACGCGTAGCGTCCCAGTGAAAGTT
GTCGATCTCATCTCGTTCTTCGCCGACCGTCTCAAAGTCCAGCTTCGCGAGCAAGGCGCGCGGCACGATCTTGTCGATGC
CGTCTTCGCCACATCACCCTCCCCGTGGTGGAGATCGGATGCGGCGAGGAGCGGTGCAGGCAGGCTTGATCTCATCGAGG
CAGCCGGCAGCGAAGAGGCCCTTGATCGTGAGCCGTCGCAGGAAGAGGAGATGAAAGACGATCTCCTGATGGTCGTGCGC
CGCGTCGAGGCGCTCGGAAAATTCCTCGACAGCGACGACGGCAAGAACCTGCTCGCCGGCACCAAGCGCGCCTCCAACAT
CCTCGCGATCGAGGAGAAGAAGGACAAGCGCAAGTTCGACGGCGCGCCGGATGCGGCGCTCTACAAGCTCGACGAGGAGA
AGGCGCTGGCCAGGGCGATCGGCGAGGTCGGCGCGGAAGCCGGCGCGGCCGTCGCCAAGGAGGATTTTGCCGCGGCGATG
CGCGCGATGGCCAAGCTCCGCCCGGCGGTCGATGCGTTCTTCGACAAGGTCAAGGTCAACGACGATGACGCCGCGATCCG
TGAGAACCGGCTGAAGCTGTTGAACGAGATCCGCAGCGCCACGCGCGCGGTGGCGGATTTCTCGAAGATCGAGGGCTGA

Upstream 100 bases:

>100_bases
TCCCGGCGCGGTTCACCCTGACGGTTCTCACAAGTGACGGCGAGTTGTCTCGCCGTGCTCCATCCTGTTTGCGCCTCACG
TCGCGCCAAGAGCGGTTGCA

Downstream 100 bases:

>100_bases
GCGGCCCTTGATCGCTTCGCTGCGCTCGAGATGAAGGAGGGGGAGCGACATCCCCCGCTGTCATGCCCGCGCAGGCGGGC
ATCCAGTACGCCGCCGCGTA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 772; Mature: 771

Protein sequence:

>772_residues
MPDLLLELFSEEIPARMQAKAADDLRRMVTDKLVAEGLVYEGAKAFATPRRLALTVHGIPARQADLKEERKGPRVGGPDA
AIQGFLKATGLASLEEATIQRDPKKGDFYVALIEKPGRATLDVLAEMLPVIVRTFPWPKSMRWGQRSEKPGALSWVRPLH
AITATFGLETEEPDVISFAVDGIEAGQTTYGHRFLAPSAFTVRRFEDYEAKLLDAKVVLDPQRRKDTIVTDAKQLAFAQG
YELVEDPVLLDEVAGLVEWPVVLMGSFDPEYLKVPGEVIRATIRNNQKCFVVRDPKTGGLAPKFILTANIEATDGGKTII
AGNERVIRARLSDAKFFYETDLKTKLEDRLPKFEQIVFHEKLGTQAARIARIEKLAAEIAPMVGADAAKTARAAKLAKAD
LLTEVVGEFPEVQGLMGKYYALAQGEDASVAAACEEHYKPQGPGDRVPADPVSVAVALADKLDTLVGFWAIDEKPTGSKD
PYALRRAALGVIRIILENDLRVHLADICGPAFFDLATAIRAREVGNEVEQAESGEKPGLTRFVVRVDAQTFGTRSVPVKV
VDLISFFADRLKVQLREQGARHDLVDAVFATSPSPWWRSDAARSGAGRLDLIEAAGSEEALDREPSQEEEMKDDLLMVVR
RVEALGKFLDSDDGKNLLAGTKRASNILAIEEKKDKRKFDGAPDAALYKLDEEKALARAIGEVGAEAGAAVAKEDFAAAM
RAMAKLRPAVDAFFDKVKVNDDDAAIRENRLKLLNEIRSATRAVADFSKIEG

Sequences:

>Translated_772_residues
MPDLLLELFSEEIPARMQAKAADDLRRMVTDKLVAEGLVYEGAKAFATPRRLALTVHGIPARQADLKEERKGPRVGGPDA
AIQGFLKATGLASLEEATIQRDPKKGDFYVALIEKPGRATLDVLAEMLPVIVRTFPWPKSMRWGQRSEKPGALSWVRPLH
AITATFGLETEEPDVISFAVDGIEAGQTTYGHRFLAPSAFTVRRFEDYEAKLLDAKVVLDPQRRKDTIVTDAKQLAFAQG
YELVEDPVLLDEVAGLVEWPVVLMGSFDPEYLKVPGEVIRATIRNNQKCFVVRDPKTGGLAPKFILTANIEATDGGKTII
AGNERVIRARLSDAKFFYETDLKTKLEDRLPKFEQIVFHEKLGTQAARIARIEKLAAEIAPMVGADAAKTARAAKLAKAD
LLTEVVGEFPEVQGLMGKYYALAQGEDASVAAACEEHYKPQGPGDRVPADPVSVAVALADKLDTLVGFWAIDEKPTGSKD
PYALRRAALGVIRIILENDLRVHLADICGPAFFDLATAIRAREVGNEVEQAESGEKPGLTRFVVRVDAQTFGTRSVPVKV
VDLISFFADRLKVQLREQGARHDLVDAVFATSPSPWWRSDAARSGAGRLDLIEAAGSEEALDREPSQEEEMKDDLLMVVR
RVEALGKFLDSDDGKNLLAGTKRASNILAIEEKKDKRKFDGAPDAALYKLDEEKALARAIGEVGAEAGAAVAKEDFAAAM
RAMAKLRPAVDAFFDKVKVNDDDAAIRENRLKLLNEIRSATRAVADFSKIEG
>Mature_771_residues
PDLLLELFSEEIPARMQAKAADDLRRMVTDKLVAEGLVYEGAKAFATPRRLALTVHGIPARQADLKEERKGPRVGGPDAA
IQGFLKATGLASLEEATIQRDPKKGDFYVALIEKPGRATLDVLAEMLPVIVRTFPWPKSMRWGQRSEKPGALSWVRPLHA
ITATFGLETEEPDVISFAVDGIEAGQTTYGHRFLAPSAFTVRRFEDYEAKLLDAKVVLDPQRRKDTIVTDAKQLAFAQGY
ELVEDPVLLDEVAGLVEWPVVLMGSFDPEYLKVPGEVIRATIRNNQKCFVVRDPKTGGLAPKFILTANIEATDGGKTIIA
GNERVIRARLSDAKFFYETDLKTKLEDRLPKFEQIVFHEKLGTQAARIARIEKLAAEIAPMVGADAAKTARAAKLAKADL
LTEVVGEFPEVQGLMGKYYALAQGEDASVAAACEEHYKPQGPGDRVPADPVSVAVALADKLDTLVGFWAIDEKPTGSKDP
YALRRAALGVIRIILENDLRVHLADICGPAFFDLATAIRAREVGNEVEQAESGEKPGLTRFVVRVDAQTFGTRSVPVKVV
DLISFFADRLKVQLREQGARHDLVDAVFATSPSPWWRSDAARSGAGRLDLIEAAGSEEALDREPSQEEEMKDDLLMVVRR
VEALGKFLDSDDGKNLLAGTKRASNILAIEEKKDKRKFDGAPDAALYKLDEEKALARAIGEVGAEAGAAVAKEDFAAAMR
AMAKLRPAVDAFFDKVKVNDDDAAIRENRLKLLNEIRSATRAVADFSKIEG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=777, Percent_Identity=34.1055341055341, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 84473; Mature: 84342

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLLLELFSEEIPARMQAKAADDLRRMVTDKLVAEGLVYEGAKAFATPRRLALTVHGIP
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEECCC
ARQADLKEERKGPRVGGPDAAIQGFLKATGLASLEEATIQRDPKKGDFYVALIEKPGRAT
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCCCCHH
LDVLAEMLPVIVRTFPWPKSMRWGQRSEKPGALSWVRPLHAITATFGLETEEPDVISFAV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEE
DGIEAGQTTYGHRFLAPSAFTVRRFEDYEAKLLDAKVVLDPQRRKDTIVTDAKQLAFAQG
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHC
YELVEDPVLLDEVAGLVEWPVVLMGSFDPEYLKVPGEVIRATIRNNQKCFVVRDPKTGGL
CHHHCCCHHHHHHHHHHHCCHHEECCCCHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCC
APKFILTANIEATDGGKTIIAGNERVIRARLSDAKFFYETDLKTKLEDRLPKFEQIVFHE
CCEEEEEEEEEECCCCCEEEECCCEEEEEECCCCCEEEECCCHHHHHHHCCHHHHHHHHH
KLGTQAARIARIEKLAAEIAPMVGADAAKTARAAKLAKADLLTEVVGEFPEVQGLMGKYY
HHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ALAQGEDASVAAACEEHYKPQGPGDRVPADPVSVAVALADKLDTLVGFWAIDEKPTGSKD
EEECCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCC
PYALRRAALGVIRIILENDLRVHLADICGPAFFDLATAIRAREVGNEVEQAESGEKPGLT
HHHHHHHHHHHHHHHHHCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
RFVVRVDAQTFGTRSVPVKVVDLISFFADRLKVQLREQGARHDLVDAVFATSPSPWWRSD
EEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
AARSGAGRLDLIEAAGSEEALDREPSQEEEMKDDLLMVVRRVEALGKFLDSDDGKNLLAG
HHCCCCCCEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
TKRASNILAIEEKKDKRKFDGAPDAALYKLDEEKALARAIGEVGAEAGAAVAKEDFAAAM
CHHCCCEEEEECCHHHHHCCCCCCHHEEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
RAMAKLRPAVDAFFDKVKVNDDDAAIRENRLKLLNEIRSATRAVADFSKIEG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDLLLELFSEEIPARMQAKAADDLRRMVTDKLVAEGLVYEGAKAFATPRRLALTVHGIP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEECCC
ARQADLKEERKGPRVGGPDAAIQGFLKATGLASLEEATIQRDPKKGDFYVALIEKPGRAT
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCCCCHH
LDVLAEMLPVIVRTFPWPKSMRWGQRSEKPGALSWVRPLHAITATFGLETEEPDVISFAV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEE
DGIEAGQTTYGHRFLAPSAFTVRRFEDYEAKLLDAKVVLDPQRRKDTIVTDAKQLAFAQG
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHC
YELVEDPVLLDEVAGLVEWPVVLMGSFDPEYLKVPGEVIRATIRNNQKCFVVRDPKTGGL
CHHHCCCHHHHHHHHHHHCCHHEECCCCHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCC
APKFILTANIEATDGGKTIIAGNERVIRARLSDAKFFYETDLKTKLEDRLPKFEQIVFHE
CCEEEEEEEEEECCCCCEEEECCCEEEEEECCCCCEEEECCCHHHHHHHCCHHHHHHHHH
KLGTQAARIARIEKLAAEIAPMVGADAAKTARAAKLAKADLLTEVVGEFPEVQGLMGKYY
HHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ALAQGEDASVAAACEEHYKPQGPGDRVPADPVSVAVALADKLDTLVGFWAIDEKPTGSKD
EEECCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCC
PYALRRAALGVIRIILENDLRVHLADICGPAFFDLATAIRAREVGNEVEQAESGEKPGLT
HHHHHHHHHHHHHHHHHCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
RFVVRVDAQTFGTRSVPVKVVDLISFFADRLKVQLREQGARHDLVDAVFATSPSPWWRSD
EEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
AARSGAGRLDLIEAAGSEEALDREPSQEEEMKDDLLMVVRRVEALGKFLDSDDGKNLLAG
HHCCCCCCEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
TKRASNILAIEEKKDKRKFDGAPDAALYKLDEEKALARAIGEVGAEAGAAVAKEDFAAAM
CHHCCCEEEEECCHHHHHCCCCCCHHEEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
RAMAKLRPAVDAFFDKVKVNDDDAAIRENRLKLLNEIRSATRAVADFSKIEG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA