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The map label for this gene is ispE [H]

Identifier: 146339068

GI number: 146339068

Start: 2153479

End: 2154381

Strand: Direct

Name: ispE [H]

Synonym: BRADO2022

Alternate gene names: 146339068

Gene position: 2153479-2154381 (Clockwise)

Preceding gene: 146339067

Following gene: 146339070

Centisome position: 28.88

GC content: 70.99

Gene sequence:

>903_bases
ATGTTGGCGTCCACGGTCACCGGCGCGCTGCAGGAGCAGGGCCGCGCCAAGGTCAATCTCACGCTGCGCGTCGTCGGCCG
CCGCGCCGACGGCTATCATGATCTCGAAAGCGTGGTGGCGTTCGCCGACTGCGCCGACCAGCTCACGCTGGCGCCGAGCC
CGGAGCTCACGCTGACCACCACCGGGCCGCTGGCGGACGCGTGCGGCGACACTTCGGACAATCTCGTGCTCAAGGCGGCG
CGCCTGCTCGCTGAGGCCGTTCCCGGCCTGACGCTCGGCGCCTTCACTCTGGAGAAGGTGCTGCCGGTGGCCGCCGGCAT
CGGCGGCGGCTCGGCCGACGCGGCGGCCGCGCTGCGGCTGCTGGCGAGGCTGAACGGTCTGTCCCTCGATGATCCGCGCC
TGCAGGCGGTGGCGCTGAAGACCGGTGCCGACGTGCCGGTCTGCGTGCCCTCGCGCGCCTGCACCATGACCGGCGTCGGC
GAGAACCTGCAGCCGCTGGCGTTGCCCGTCCTGCCGTGCGTGATGATCAACCCGCGCGTGCCGGTCGCGACCAAGGACGT
GTTCCAGGCGCTCGGGCTGAAGCCGGGCGATCTGCTGGTCGGGGTGAGCGACGTGCTGGCGGCGCCAGCGTGGCCGAAGG
CTGGCGCGTCGATTGGCGATTGGGTCGCGGCGCTCGACCAGGTGAAGAACGATCTCGAGCCCCCGGCGCTGAAGGTGCAG
CCGATCATCGGTACCGTGCTCGATGCGCTGCGTGCGAGCAACGGAGTGCTGCTGGCGCGGATGTCCGGCTCCGGCGCGAC
GTGCTTTGCGATCTATGGCGGCGATGCTGATGCGAAGATCGCGGGTGCCGCGATCGCAGCGGCGCATCCCGAATGGTGGG
TGCATGCGGGCACGCTGAGCTAA

Upstream 100 bases:

>100_bases
GATTTTGATGAAGGTGTGGTGCTGCCGGCCACGTTCGTTGCGGCCTCCGGCTGACGGCGTGAAGGCATCCACCACGGACT
AATTAGGGGGCATTGCGCCG

Downstream 100 bases:

>100_bases
CTAATTAATCGCCGCGAAACGACTATCCAATACAGCGCCCTCGCCCCTTGTGGGAGAGGGCATGTATGACAGATCAACGA
GCTCGCTTGGGTGAGGGGTC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MLASTVTGALQEQGRAKVNLTLRVVGRRADGYHDLESVVAFADCADQLTLAPSPELTLTTTGPLADACGDTSDNLVLKAA
RLLAEAVPGLTLGAFTLEKVLPVAAGIGGGSADAAAALRLLARLNGLSLDDPRLQAVALKTGADVPVCVPSRACTMTGVG
ENLQPLALPVLPCVMINPRVPVATKDVFQALGLKPGDLLVGVSDVLAAPAWPKAGASIGDWVAALDQVKNDLEPPALKVQ
PIIGTVLDALRASNGVLLARMSGSGATCFAIYGGDADAKIAGAAIAAAHPEWWVHAGTLS

Sequences:

>Translated_300_residues
MLASTVTGALQEQGRAKVNLTLRVVGRRADGYHDLESVVAFADCADQLTLAPSPELTLTTTGPLADACGDTSDNLVLKAA
RLLAEAVPGLTLGAFTLEKVLPVAAGIGGGSADAAAALRLLARLNGLSLDDPRLQAVALKTGADVPVCVPSRACTMTGVG
ENLQPLALPVLPCVMINPRVPVATKDVFQALGLKPGDLLVGVSDVLAAPAWPKAGASIGDWVAALDQVKNDLEPPALKVQ
PIIGTVLDALRASNGVLLARMSGSGATCFAIYGGDADAKIAGAAIAAAHPEWWVHAGTLS
>Mature_300_residues
MLASTVTGALQEQGRAKVNLTLRVVGRRADGYHDLESVVAFADCADQLTLAPSPELTLTTTGPLADACGDTSDNLVLKAA
RLLAEAVPGLTLGAFTLEKVLPVAAGIGGGSADAAAALRLLARLNGLSLDDPRLQAVALKTGADVPVCVPSRACTMTGVG
ENLQPLALPVLPCVMINPRVPVATKDVFQALGLKPGDLLVGVSDVLAAPAWPKAGASIGDWVAALDQVKNDLEPPALKVQ
PIIGTVLDALRASNGVLLARMSGSGATCFAIYGGDADAKIAGAAIAAAHPEWWVHAGTLS

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=285, Percent_Identity=29.4736842105263, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 30438; Mature: 30438

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLASTVTGALQEQGRAKVNLTLRVVGRRADGYHDLESVVAFADCADQLTLAPSPELTLTT
CCCCHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCEEECCCCCEEEEE
TGPLADACGDTSDNLVLKAARLLAEAVPGLTLGAFTLEKVLPVAAGIGGGSADAAAALRL
CCCCHHHCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LARLNGLSLDDPRLQAVALKTGADVPVCVPSRACTMTGVGENLQPLALPVLPCVMINPRV
HHHHCCCCCCCCCEEEEEEECCCCCEEECCCCCEEECCCCCCCCHHHHHHHCEEEECCCC
PVATKDVFQALGLKPGDLLVGVSDVLAAPAWPKAGASIGDWVAALDQVKNDLEPPALKVQ
CCCHHHHHHHHCCCCCCEEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEH
PIIGTVLDALRASNGVLLARMSGSGATCFAIYGGDADAKIAGAAIAAAHPEWWVHAGTLS
HHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHEEEECCCCCEEEECCCC
>Mature Secondary Structure
MLASTVTGALQEQGRAKVNLTLRVVGRRADGYHDLESVVAFADCADQLTLAPSPELTLTT
CCCCHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCEEECCCCCEEEEE
TGPLADACGDTSDNLVLKAARLLAEAVPGLTLGAFTLEKVLPVAAGIGGGSADAAAALRL
CCCCHHHCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LARLNGLSLDDPRLQAVALKTGADVPVCVPSRACTMTGVGENLQPLALPVLPCVMINPRV
HHHHCCCCCCCCCEEEEEEECCCCCEEECCCCCEEECCCCCCCCHHHHHHHCEEEECCCC
PVATKDVFQALGLKPGDLLVGVSDVLAAPAWPKAGASIGDWVAALDQVKNDLEPPALKVQ
CCCHHHHHHHHCCCCCCEEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEH
PIIGTVLDALRASNGVLLARMSGSGATCFAIYGGDADAKIAGAAIAAAHPEWWVHAGTLS
HHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHEEEECCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA