Click here to switch to the map view.

The map label for this gene is usf [H]

Identifier: 146339035

GI number: 146339035

Start: 2110648

End: 2111322

Strand: Direct

Name: usf [H]

Synonym: BRADO1986

Alternate gene names: 146339035

Gene position: 2110648-2111322 (Clockwise)

Preceding gene: 146339034

Following gene: 146339036

Centisome position: 28.31

GC content: 67.56

Gene sequence:

>675_bases
ATGGGCACACTCGTCAGCTTCAAGCGGCCCGACGGCCAGGAAGCCAAGGGCTATCTCGCCAAGGCGGCCAAGGCCGATGC
GCCCGGCGTCGTCGTCATTCAGGAATGGTGGGGCCTGCAGGACCAGATCAAGGGCATGGCCGACCGCTTCGCGCTGGCCG
GCTTCAACGCCCTGGCGCCCGACCTCTACAAGGGCATGGTGGTGCCGTATCACGACACGGACGCGGCGGGCCGCGAGATG
AACTCGCTCGACTTCATGGATGCGACGACGCAGACCGTGCGCGGCGCCGCGCAATATCTGGCGCGCAACGGCGCCAAGGT
CGGCCTGACCGGCTTCTGCCTCGGCGGCGCCGTGACGATCATCGGCGCCACCAAGATCGACGAGCTCGCGGCCGGCGTTG
TGTTCTATGGCATTCCGCCGGAGCAGGCGGCGAAGCCCGCCGACGTCAAGATCCCGCTGCAGGCGCACTTCGCCAATAAG
GACGATTGGTGCACGCCGGCGCTGGTGGATGGCTTCGAGGCGGCGATGAAGGCGGCCGGCAAGTCGCTCGAGCTGTATCG
CTACGACGCCGAGCATGCCTTCGTGAACGAGCAGCGCCTGACCGTCCATGATCGTGCGGCCGCCGAGCTGGCCTGGGGCC
GCGCCACGGCGTTCTTCCACAAGCATCTCGGATGA

Upstream 100 bases:

>100_bases
CCCACGACGCGATCGCCATGCTGCCAGGCCAGTCGCGCCTGTGATGTATTTGCGCTAAACCGCAGTCCCAAGTCCCGATC
AGAAACTGCGGAGGATCATC

Downstream 100 bases:

>100_bases
GGTGAGGGGAGCGGCTCGTCGCCCTTGCCGTCGCACCGCGCGGCGTCACACCGCGCGGGCGGTCTTCTCGGCGGCTTCGT
CCGGCAGGGGCTCGGCATCA

Product: putative carboxymethylenebutenolidase

Products: oxohex-2-enedioate [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MGTLVSFKRPDGQEAKGYLAKAAKADAPGVVVIQEWWGLQDQIKGMADRFALAGFNALAPDLYKGMVVPYHDTDAAGREM
NSLDFMDATTQTVRGAAQYLARNGAKVGLTGFCLGGAVTIIGATKIDELAAGVVFYGIPPEQAAKPADVKIPLQAHFANK
DDWCTPALVDGFEAAMKAAGKSLELYRYDAEHAFVNEQRLTVHDRAAAELAWGRATAFFHKHLG

Sequences:

>Translated_224_residues
MGTLVSFKRPDGQEAKGYLAKAAKADAPGVVVIQEWWGLQDQIKGMADRFALAGFNALAPDLYKGMVVPYHDTDAAGREM
NSLDFMDATTQTVRGAAQYLARNGAKVGLTGFCLGGAVTIIGATKIDELAAGVVFYGIPPEQAAKPADVKIPLQAHFANK
DDWCTPALVDGFEAAMKAAGKSLELYRYDAEHAFVNEQRLTVHDRAAAELAWGRATAFFHKHLG
>Mature_223_residues
GTLVSFKRPDGQEAKGYLAKAAKADAPGVVVIQEWWGLQDQIKGMADRFALAGFNALAPDLYKGMVVPYHDTDAAGREMN
SLDFMDATTQTVRGAAQYLARNGAKVGLTGFCLGGAVTIIGATKIDELAAGVVFYGIPPEQAAKPADVKIPLQAHFANKD
DWCTPALVDGFEAAMKAAGKSLELYRYDAEHAFVNEQRLTVHDRAAAELAWGRATAFFHKHLG

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994982, Length=243, Percent_Identity=27.9835390946502, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: 3.1.1.45 [C]

Molecular weight: Translated: 24059; Mature: 23927

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTLVSFKRPDGQEAKGYLAKAAKADAPGVVVIQEWWGLQDQIKGMADRFALAGFNALAP
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHCCHHHCH
DLYKGMVVPYHDTDAAGREMNSLDFMDATTQTVRGAAQYLARNGAKVGLTGFCLGGAVTI
HHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCEEEE
IGATKIDELAAGVVFYGIPPEQAAKPADVKIPLQAHFANKDDWCTPALVDGFEAAMKAAG
EECCHHHHHHCCEEEECCCHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
KSLELYRYDAEHAFVNEQRLTVHDRAAAELAWGRATAFFHKHLG
CCEEEEEECCCHHEECCCEEEEEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GTLVSFKRPDGQEAKGYLAKAAKADAPGVVVIQEWWGLQDQIKGMADRFALAGFNALAP
CCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHCCHHHCH
DLYKGMVVPYHDTDAAGREMNSLDFMDATTQTVRGAAQYLARNGAKVGLTGFCLGGAVTI
HHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCEEEE
IGATKIDELAAGVVFYGIPPEQAAKPADVKIPLQAHFANKDDWCTPALVDGFEAAMKAAG
EECCHHHHHHCCEEEECCCHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
KSLELYRYDAEHAFVNEQRLTVHDRAAAELAWGRATAFFHKHLG
CCEEEEEECCCHHEECCCEEEEEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-carboxymethylenebut-2-en-4-olide; H2O [C]

Specific reaction: 4-carboxymethylenebut-2-en-4-olide + H2O = 4 oxohex-2-enedioate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592443 [H]