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The map label for this gene is cca [H]

Identifier: 146339019

GI number: 146339019

Start: 2093882

End: 2095135

Strand: Direct

Name: cca [H]

Synonym: BRADO1968

Alternate gene names: 146339019

Gene position: 2093882-2095135 (Clockwise)

Preceding gene: 146339018

Following gene: 146339020

Centisome position: 28.08

GC content: 70.02

Gene sequence:

>1254_bases
ATGACCGGCGCGCGGGTGCTGAGCGCGCCCTGGCTCTCGTCCGGCCCGGCGGCGCGGGTGCTCGCCTTGCTGAATTGCGA
TGGCGAGGAGGCACGCGTGGTCGGCGGTGCCGTGCGCAACGCGCTGCTCGGCGTTGCGATTGGCGATATCGACATCGCCA
CCACGGCGCTGCCTCCCGAGGTGATCCGCCGCGCCAAGGCCGCCTCGATCAAGAGCGTGCCGACCGGCATCGACCACGGC
ACCATCACGCTGGTGCTCGACGGTCATCCGTTCGAGGTCACCACCTTGCGCGAGGACACCGAGACATTTGGCCGCAAGGC
GAAGGTCGCCTTCGGCCGCGACTGGGTCGGCGACGCGCACCGCCGCGACTTCACCATCAATGCGCTGTCGGTGGGGGCCG
ACGGCGTCGTGCACGATCATGTCGGCGGGCTCGCAGATATCGAGGCCCGGCGGGTGCGCTTCATCGGCGATCCCTCCCAG
CGCATCGCTGAGGATTACTTGCGCATCCTGCGCTTCTTCCGCATTCACTCGGCGTTCGGTCATGGCGAGCCCGATCGCGA
GGGCTATCTCGCCTGCATCGCCGGACGTGGCGGGCTCGCGACGCTATCGGCCGAGCGGGTGCGCATGGAGATGCTCAAGC
TGCTCGTGACCGAGGGCGCAGTCGGCGCGGTCACCGCCATGGCCGATGGCGGCCTGCTGACGTCGATCCTCGGCGGCGTC
GCCTATGTCGGCCCCTTTGCGGCAATGATCGACATCGAGCGCGAGCTTTCGCTGCCGCCGAGCCCCGTGCGCCGCCTCGC
GGCGCTCGCGGTCGCCGTCACGGAGGATGCGCGCCGTCTCGGGGCGCGGCTGAAGCTGTCCAATGCCGAGACCAAGGCGC
TCGACGGCATGGGCCACCGCTGGTGGCGCTTCGCGTCCGCCGACGAGGCGCGGGGGCGGCGGCTGCTGTATCGCCTCGGC
GAGACGGTCTATCGCGATCGCCTGATGCTCGGTTGGGCGCGCAGCGGCTGTCGTCGCGACGCGCAGCATTGGCGCGAACT
GGCGCTGTTGCCGCAGCGCTTCACCCCGCCGAAATTGCCGCTGAAAGCGGCCGATTTCATCGCGCGCGGGGTTGCGGAGG
GGCCGCTGCTCGGTCATGTGCTGACTCTCGCAGAGGATGCCTGGCTGGCTGCGGACTTTCCGCTCGACGAGGCATCGCTG
ACCGCGATCGCCGATCAGGCGGCGGCGCGCGTCAACCGCGACGGACGTTCATGA

Upstream 100 bases:

>100_bases
CAAGTCTGGTCATGGTCGCCGAATTGTCCGGCGCTCCGCCGCATTCGACCTATGTGCCCGCTCACATCGAGAACGGCCGG
CTCGTTCCGGGAGCCGAGAA

Downstream 100 bases:

>100_bases
CTTTTCTGGCCGGCTTTGCCGACATCTCTCTTCTGCAGCTCCTGCTCGTCGCGTCCGTCGCCTTATTTGCTTCCATCATC
GGTGGTCTCGCCGGCTACGG

Product: putative poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MTGARVLSAPWLSSGPAARVLALLNCDGEEARVVGGAVRNALLGVAIGDIDIATTALPPEVIRRAKAASIKSVPTGIDHG
TITLVLDGHPFEVTTLREDTETFGRKAKVAFGRDWVGDAHRRDFTINALSVGADGVVHDHVGGLADIEARRVRFIGDPSQ
RIAEDYLRILRFFRIHSAFGHGEPDREGYLACIAGRGGLATLSAERVRMEMLKLLVTEGAVGAVTAMADGGLLTSILGGV
AYVGPFAAMIDIERELSLPPSPVRRLAALAVAVTEDARRLGARLKLSNAETKALDGMGHRWWRFASADEARGRRLLYRLG
ETVYRDRLMLGWARSGCRRDAQHWRELALLPQRFTPPKLPLKAADFIARGVAEGPLLGHVLTLAEDAWLAADFPLDEASL
TAIADQAAARVNRDGRS

Sequences:

>Translated_417_residues
MTGARVLSAPWLSSGPAARVLALLNCDGEEARVVGGAVRNALLGVAIGDIDIATTALPPEVIRRAKAASIKSVPTGIDHG
TITLVLDGHPFEVTTLREDTETFGRKAKVAFGRDWVGDAHRRDFTINALSVGADGVVHDHVGGLADIEARRVRFIGDPSQ
RIAEDYLRILRFFRIHSAFGHGEPDREGYLACIAGRGGLATLSAERVRMEMLKLLVTEGAVGAVTAMADGGLLTSILGGV
AYVGPFAAMIDIERELSLPPSPVRRLAALAVAVTEDARRLGARLKLSNAETKALDGMGHRWWRFASADEARGRRLLYRLG
ETVYRDRLMLGWARSGCRRDAQHWRELALLPQRFTPPKLPLKAADFIARGVAEGPLLGHVLTLAEDAWLAADFPLDEASL
TAIADQAAARVNRDGRS
>Mature_416_residues
TGARVLSAPWLSSGPAARVLALLNCDGEEARVVGGAVRNALLGVAIGDIDIATTALPPEVIRRAKAASIKSVPTGIDHGT
ITLVLDGHPFEVTTLREDTETFGRKAKVAFGRDWVGDAHRRDFTINALSVGADGVVHDHVGGLADIEARRVRFIGDPSQR
IAEDYLRILRFFRIHSAFGHGEPDREGYLACIAGRGGLATLSAERVRMEMLKLLVTEGAVGAVTAMADGGLLTSILGGVA
YVGPFAAMIDIERELSLPPSPVRRLAALAVAVTEDARRLGARLKLSNAETKALDGMGHRWWRFASADEARGRRLLYRLGE
TVYRDRLMLGWARSGCRRDAQHWRELALLPQRFTPPKLPLKAADFIARGVAEGPLLGHVLTLAEDAWLAADFPLDEASLT
AIADQAAARVNRDGRS

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=391, Percent_Identity=32.2250639386189, Blast_Score=170, Evalue=3e-42,
Organism=Escherichia coli, GI87081691, Length=178, Percent_Identity=35.3932584269663, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=391, Percent_Identity=31.9693094629156, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI281360112, Length=276, Percent_Identity=37.6811594202899, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21357337, Length=276, Percent_Identity=37.6811594202899, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 44931; Mature: 44800

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGARVLSAPWLSSGPAARVLALLNCDGEEARVVGGAVRNALLGVAIGDIDIATTALPPE
CCCCCEEECCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHEEECCCEECCCCCCHH
VIRRAKAASIKSVPTGIDHGTITLVLDGHPFEVTTLREDTETFGRKAKVAFGRDWVGDAH
HHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEECHHHHHHCCEEEEEECCCCCCCCC
RRDFTINALSVGADGVVHDHVGGLADIEARRVRFIGDPSQRIAEDYLRILRFFRIHSAFG
CCCEEEEEEEECCCCEEHHHCCCCCCCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHC
HGEPDREGYLACIAGRGGLATLSAERVRMEMLKLLVTEGAVGAVTAMADGGLLTSILGGV
CCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEHHCCCCHHHHHHHHH
AYVGPFAAMIDIERELSLPPSPVRRLAALAVAVTEDARRLGARLKLSNAETKALDGMGHR
HHHHHHHHHEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHCCCCCH
WWRFASADEARGRRLLYRLGETVYRDRLMLGWARSGCRRDAQHWRELALLPQRFTPPKLP
HEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LKAADFIARGVAEGPLLGHVLTLAEDAWLAADFPLDEASLTAIADQAAARVNRDGRS
HHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TGARVLSAPWLSSGPAARVLALLNCDGEEARVVGGAVRNALLGVAIGDIDIATTALPPE
CCCCEEECCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHEEECCCEECCCCCCHH
VIRRAKAASIKSVPTGIDHGTITLVLDGHPFEVTTLREDTETFGRKAKVAFGRDWVGDAH
HHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEECHHHHHHCCEEEEEECCCCCCCCC
RRDFTINALSVGADGVVHDHVGGLADIEARRVRFIGDPSQRIAEDYLRILRFFRIHSAFG
CCCEEEEEEEECCCCEEHHHCCCCCCCCHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHC
HGEPDREGYLACIAGRGGLATLSAERVRMEMLKLLVTEGAVGAVTAMADGGLLTSILGGV
CCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEHHCCCCHHHHHHHHH
AYVGPFAAMIDIERELSLPPSPVRRLAALAVAVTEDARRLGARLKLSNAETKALDGMGHR
HHHHHHHHHEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHCCCCCH
WWRFASADEARGRRLLYRLGETVYRDRLMLGWARSGCRRDAQHWRELALLPQRFTPPKLP
HEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LKAADFIARGVAEGPLLGHVLTLAEDAWLAADFPLDEASLTAIADQAAARVNRDGRS
HHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA